Abstract
Two species of Cantellius from Taiwan were identified from the elephant skin coral, Pachyseris speciosa Dana, 1846. Cantellius sextus was found on the underside of the coral plate and a new species, C. hoegi sp. nov., on the upper surface. The microdistribution indicates the different requirements of the two species. In the present study, both arthropodal and opercular plate characters of these two species were investigated using SEM techniques. Morphologically, C. hoegi is close to C. pallidus. However, the tergal scutal margins of C. pallidus are curved, whilst those of C. hoegi are straight, and its spur is narrower than that of C. pallidus. The apex of the scutum and tergum of C. hoegi forms a honeycomb-like surface, most probably the ‘imprint’ of the chitinous primordial valves. The separation of C. hoegi from C. pallidus is also supported by molecular analyses of three mitochondrial gene sequences (COI, 16S, and 12S rRNA). Cantellius hoegi differs from C. pallidus and C. sextus by >5% in all three mitochondrial genes (COI, 16S, and 12S rRNA) sequenced. The values of sequence divergence in 12S are comparable to the inter-specific divergence reported for other Cantellius and Savignium species. Interestingly, C. hoegi is not the sister group of C. pallidus, a morphologically similar species, in the present study. Further studies should focus on molecular phylogenetic analysis of Cantellius species.References
Achituv, Y. (2001) Cantellius alphonsei n. sp., a new coral-inhabiting barnacle (Cirripedia, Pyrgomatinae) of Montipora. Crustaceana, 74, 217–226.
Achituv, Y. (2004) Coral-Inhabiting Barnacles (Cirripedia; Balanomorpha; Pyrgomatinae) from the Kermadec Islands and Niue Island, New Zealand. New Zealand Journal of Marine and Freshwater Research, 8, 43–49.
Achituv, Y. & Newman, W.A. (2002) The barnacles of Astreopora (Cirripedia, Pyrgomatini/Scleractinia, Acroporidae); organization plans, host specificity, species-richness, and geographic range. Journal of Natural History [in press]
Achituv, Y. & Hoeksema, B. (2003) Cantellius cardenae n. sp., (Cirripedia: Pyrgomatinae) from Acropora brueggmanni (Anthozoa: Acroporidae), a case of species specificity in a generalist genus. Zoologische Mededelingen Leiden, 77, 1–8.
Achituv, Y. & Langsam, Y. (2004) Coral-inhabiting barnacles (Cirripedia; Thoracica; Balanomorpha; Pyrgomatinae) from east of 150° West. Journal of Natural History, 39, 1705–1717.
Broch, H. (1931) Papers from Dr Th. Mortensn's Pacific expedition 1914–1916 LVI Indomalayan Cirripedia. Videnskabelige Meddelser fra Dansk Naturhistorisk Forening i Kjobenhaven, 91, 1–146.
Chan, B.K.K., Tsang, L.M. & Chu, K.H. (2007a) Morphological and genetic differentiation of Tetraclita squamosa (Crustacea, Cirripedia) in East Asia and description of a new species of Tetraclita. Zoological Scripta, 36, 79–91.
Chan, B.K.K., Tsang, L.M. & Chu, K.H. (2007b) Cryptic diversity of Tetraclita squamosa complex (Crustacea, Cirripedia) in Asia: description of a new species from Singapore. Zoological Studies, 46, 46–56.
Chan, B.K.K., Høeg, J.T. & Garm, A. (2008a) Setal morphology and setation patterns of barnacle cirri: adaptations and implications for thoracican evolution. Journal of Zoology (London), 275, 294–306.
Chan, B.K.K., Hsu, C.-H. & Southward, A. J. (2008b) Morphological variation and biogeography of an insular intertidal barnacle Hexechamaesipho pilsbryi (Crustacea: Cirripedia) in the western Pacific. Bulletin of Marine Science, 83, 315–328.
Crandall, K.A. & Fitzpatrick, J. F. (1996) Crayfish molecular systematics: using a combination of procedures to estimate phylogeny. Systematics Biology, 45, 1–26.
Darwin, C. (1851) A monograph on the sub-class Cirripedia with figures of all the species. The Lepadidae. Ray Society, London, 400 pp.
Darwin, C. (1854) A monograph on the sub-class Cirripedia with figures of all the species. The Balanidae the verrucidae etc. Ray Society, London, 684 pp.
Farris, J.S., Kallersjo, M., Kluge, A.G. & Bult, C. (1994) Testing significance of incongruence. Cladistics, 10, 315–319.
Folmer, O., Black, M., Hoeh, W., Lutz, R.A. & Vrijenhoek, R.C. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3, 294–299.
Hiro, F. (1935) A study of cirripeds associated with corals occurring in Tanabe Bay. Records of Oceanographic Works in Japan, 7, 1–28.
Hiro, F. (1938) Studies on animals inhabiting reef corals II. Cirripeds of the genera Creusia and Pyrgoma. Palao Tropical Biological Station Studies, 3, 391–416.
Huelsenbeck, J.P. & Ronquist, F. (2001) MRBAYES: Bayesian inference of phylogeny. Bioinformatics, 17, 754–755.
Kocher, T.D., Thomas, W.K., Meyer, A., Edwards, S.V., Pääbo, S., Villablanca, F.X., Wilson, A.C. (1989) Dynamic of mitochondrial evolution in animals: amplification and sequencing with conserve primers. Proceedings of National Academy of Sciences USA, 86, 6196–6200.
Mokady, O. & Brickner, I. (2001) Host-associated speciation in a coral-inhabiting barnacle. Molecular Biology and Evolution, 18, 975–981.
Mokady, O., Rozenblatt, S., Graur, D. & Loya, Y. (1994) Coral-host specificity of Red Sea Lithophaga bivalves: Interspecific and intraspecific variation in 12S mitochondrial ribosomal RNA. Molecular Marine Biology and Biotechnology, 3, 158–164.
Mokady, O., Loya, Y., Achituv, Y., Geffen, E., Rozenblatt, S. & Brickner, I. (1999) Speciation versus phenotypic plasticity in coral inhabiting barnacles: Darwin’s observations in an ecological context. Journal of Molecular Evolution, 49, 367–375.
Newman W. A. (1987) Evolution of cirripededs and their major groups. In: Southward A.J. (Ed.), Barnacle Biology. Balkema, Rotterdam, pp. 3–42.
Newman, W.A., Zullo V.A. & Withers T.H. (1969) Cirripedia. In: Moore R.C. (Ed.), Treatise on Invertebrate paleontology Part R Arthropoda 4. University of Kansas & Geological Society of America. Lawrence, Kansas, pp. R628–R295.
Ogawa, K. & Matsuzaki, K. (1992) An assay on host specificity, systematic taxonomy and evolution of the coral-barnacles. Bulletin of the Biogeographical Society of Japan, 47, 1–16.
Posada, D. & Crandall, K.A. (1998) MODELTEST: testing the model of DNA substitution. Bioinformatics, 14, 817–818.
Ross, A. & Newman, W.A. (1973) Revision of the coral-inhabiting barnacles (Cirripedia: Balanidae). Transactions of the San Diego Society of Natural History, 17, 137–173.
Ross, A. & Newman, W.A. (2000) Coral barnacles: Coenozoic decline and extinction in the Atlantic/East Pacific versus diversification in the Indo Pacific. Proceedings of the 9th International Coral Reef Symposium. Bali Indonesia.
Simon, C., Frati, F., Beckenbach, A., Crespi, B., Liu, H. & Flook, P. (1994) Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Annuals of the Entomological Society of America, 87, 652–70.
Soong, K.Y. & Chang, K.H. (1983) The coral-inhabiting barnacles (Crustacea: Thoracica: Pyrgomatidae) from southern most coast of Taiwan. Bulletin of the Institute Zoology Academia Sinica, 22, 243–253.
Swofford, D.L. (2000). PAUP*: phylogenetic analysis using parsimony (*and other methods), ver 4. Sinauer, Sunderland, MA.
Thompson, J.D., Higgins, D.G. & Gibson, T.J. (1994) Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Proceedings of the National Academy of Science of the USA, 22, 4673–4680.
Veron, J.E.N. (2000) Corals of the World, Vol. 2. Australian Institute of Marine Research, Townsville, pp. 224–232.