Skip to main content Skip to main navigation menu Skip to site footer
Type: Article
Published: 2003-05-04
Page range: 1–16
Abstract views: 38
PDF downloaded: 8

Molecular taxonomy of two sympatric sibling species of the pollenbeetle genus Meligethes (Coleoptera: Nitidulidae)

Dipartimento di Biologia Animale e dell’Uomo, Università degli Studi di Roma “La Sapienza”, Viale dell’Università 32, I-00185 Rome (Italy)
Dipartimento di Biologia Animale e dell’Uomo, Università degli Studi di Roma “La Sapienza”, Viale dell’Università 32, I-00185 Rome (Italy)
Dipartimento di Biologia Animale e dell’Uomo, Università degli Studi di Roma “La Sapienza”, Viale dell’Università 32, I-00185 Rome (Italy)
Dipartimento di Biologia Animale e dell’Uomo, Università degli Studi di Roma “La Sapienza”, Viale dell’Università 32, I-00185 Rome (Italy)
Coleoptera Nitidulidae Meligethinae Meligethes molecular taxonomy resurrected species

Abstract

A molecular analysis was performed, combined with field data on insect/host-plant associations, in order to clarify taxonomic relationships within the W-Palaearctic Meligethes aeneus complex (Coleoptera, Nitidulidae, Meligethinae). The analysis was focused on the specific distinction of M. aeneus (Fabricius, 1775), and M. gracilis C.Brisout de Barneville, 1863, with special regard to populations occurring in sympatry. The genetic divergence between the two examined taxa, based on comparison between mtDNA sequence data, was congruent with distance values usually scored between related, but evidently separated, insect species. Genetic data and ecological evidence clearly show that Meligethes aeneus and M. gracilis, erroneously synonymized in a recent paper by a Russian author, are distinct biological species that frequently occur in syntopy in Western Europe.

References

  1. Audisio, P. (1993) Coleoptera Nitidulidae - Kateretidae. Fauna d'Italia, vol. 32, Calderini Editore, Bologna, XVI + 971 pp.

    Audisio, P., Belfiore, C., De Biase, A. & Antonini, G. (2001a) Identification of Meligethes matronalis Audisio and Spornraft, 1990 and M. subaeneus Sturm, 1845, based on morphometric and bionomic characters (Coleoptera: Nitidulidae). European Journal of Entomology, 98, 87-97.

    Audisio, P. & De Biase, A. (1999) Bionomic and morphological evidence of a new southern European species of the Meligethes viridescens complex (Coleoptera: Nitidulidae). Folia Heyrovskyana, 7(2), 99-113.

    Audisio, P., De Biase, A. & Antonini, G. (in press) A new exceptional Meligethes of the M. aeneus species-group from Western Alps and an updated key to identification of M. aeneus and allied species (Coleoptera: Nitidulidae: Meligethinae). Insect Systematics and Evolution.

    Audisio, P., De Biase, A., Antonini, G., Belfiore, C. & Oliverio, M. (2001b) Morphological, molecular, and ecological evidence of a new Euro-Anatolian species of the Meligethes coracinus complex (Coleoptera: Nitidulidae). Insect Systematics and Evolution, 31, 361-385.

    Audisio, P., De Biase, A., Romanelli, P., Angelici, M.C., Ketmaier V. & De Matthaeis, E. (1999a) Molecular re-examination of the taxonomy of the Meligethes viridescens species-complex (Coleoptera: Nitidulidae). Biochemical Systematics and Ecology, 28, 1-13.

    Audisio, P., Jelínek, J., Mariotti, A. & De Biase, A. (2000) The Coleoptera Nitidulidae and Kateretidae from Anatolian, Caucasian and Middle East regions. Biogeographia, Lavori della Società italiana di Biogeografia, 21, 241-354.

    Audisio, P., Jelínek, J. & Stevanovic, M. (1999b) Morphological and biogeographical reexamination of the Meligethes squamosus species complex (Coleoptera: Nitidulidae: Meligethinae). Folia Heyrovskyana, 7(1), 61-71.

    Bonfield, J., Beal, K., Jordan, M., Cheng, Y. & Staden, R. (1999-2002) The Staden Package Manual. Medical Research Council, Laboratory of Molecular Biology, Cambridge, U.K.

    Brower, A.V.Z. (1994) Rapid morphological radiation and convergence among races of the butterfly Heliconius erato inferred from patterns of mitochondrial DNA evolution. Proceedings of the National Academy of Sciences of the United States of America, 91, 64691-6495.

    Clary, D.O. & Wolstenholme, D.R. (1985) The mitochondrial DNA molecule of Drosophila yakuba: nucleotide sequence, gene organization, and genetic code. Journal of Molecular Evolution, 22, 252-271.

    De Bruijn, M.H.L. (1983) Drosophila melanogaster mitochondrial DNA: a novel organization and genetic code. Nature, 304, 234-241.

    DeSalle, R., Freedman, T., Prager, E.M. & Wilson, A.C. (1987) Tempo and mode of sequence evolution in mitochondrial DNA of Hawaiian Drosophila. Journal of Molecular Evolution, 26, 157-164.

    Easton, A. M. (1959) A new Nearctic species of Meligethes (Col., Nitidulidae). Entomologist’s Monthly Magazine, 95, 58-61.

    Funk D.J., 1999. Molecular systematics of cytochrome oxidase I and 16S from Neochlamisus leaf beetles and the importance of sampling. Molecular Biology and Evolution, 16(1), 67-82.

    Fukatsu, T. (1999) Acetone preservation: a practical technique for molecular analysis. Molecular Ecology, 8, 1935-1945.

    Jelínek, J. (1982) New palaearctic species of the genus Meligethes (Coleoptera, Nitidulidae). Acta entomologica bohemoslovaca, 79, 289-300.

    Juan, C., Oromì, P. & Hewitt, G.M. (1995) Mitochondrial DNA phylogeny and sequential colonization of Canary Islands by darkling beetles of the genus Pimelia (Tenebrionidae). Proceedings of the Royal Society of London B, 261, 173-180.

    Judd, W.S., Sanders, R.W. & Donoghue, M.J. (1994) Angiosperm family pairs: preliminary cladistic analyses. Harward Papers of Botany, 5, 1-51.

    Kimura, M. (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16, 111-120.

    Kirejtshuk, A. G. (1979) The Nitidulid-beetles of the subfamily Meligethinae (Coleoptera, Nitidulidae) of the SSSR fauna. (In russian). Ph.D. Dissertation Thesis Abstract, Zoological Institute of the Russian Academy of Sciences, 1979, 1-24.

    Kirejtshuk, A. G. (1997) New Palaearctic nitidulid beetles, with notes on synonymy and systematic position of some species (Coleoptera: Nitidulidae). Zoosystematica Rossica, 6, 255-268.

    Kumar, S., Tamura, K., Jakobsen, I.B. & Nei, M. (2001) MEGA2: Molecular Evolutionary Genetics Analysis software, Arizona State University, Tempe, Arizona, USA.

    Liu, H. & Beckenbach, A.T. (1992) Evolution on the mitochondrial cytochrome oxidase II gene among 10 orders on insects. Molecular Phylogenetic and Evolution, 1, 41-52.

    Lunt, D.H., Zhang, D.-X., Szymura, J.M. & Hewitt, G.M. (1996) The insect cytochrome oxidase I gene: evolutionary patterns and conserved primers for phylogenetic studies. Insect Molecular Biology, 5, 153-165.

    Maddison, W.R. & Maddison, D.R. (1992) MacClade: Analysis of Phylogeny and Character Evolution. Version 3. Sinauer Associates Inc., Sunderland, Massachusetts, XI+398 pp.

    Mardulyn, P., Termonia, A. & Milinkovitch, M.C. (2003) Structure and evolution of the mitochondrial control region of leaf beetles (Coleoptera: Chrysomelidae): A hierarchical analysis of nucleotide sequence variation. Journal of Molecular Evolution, 56, 38-45.

    Rozas, J. & Rozas, R. (1999) DnaSP version 3: an integrated program for molecular population genetics and molecular evolution analysis. Bioinformatics, 15, 174-175.

    Saiki, R. K., Scharf, S., Faloona, F., Mullis, K. B., Horn, G. T., Erlich, H. A. & Arnheim, N. (1985) Enzymatic amplification of b-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science (Washington, DC), 230, 1350-1354.

    Saitou, N. & Nei, M. (1987) The neighbour-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4, 406-425.

    Sambrook, J. & Russell, D. (2000) Molecular Cloning. A Laboratory Manual. Third edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 999 pp.

    Simon, C., Frati, F., Beckenbach, A., Crespi, B., Liu, H. & Flook, P. (1994) Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Annals of the Entomological Society of America, 87(6), 651- 701.

    Vogler, A.P., DeSalle, R., Assman, T., Knisley, C.B. & Schultz, T.D. (1993). Molecular population genetics of the endangered tiger beetle, Cicindela dorsalis (Coleoptera: Cicindelidae). Annals of the Entomological Society of America, 86, 142-152.