Skip to main content Skip to main navigation menu Skip to site footer
Type: Article
Published: 2008-08-20
Page range: 57–67
Abstract views: 36
PDF downloaded: 1

Molecular Identification of Delphinids and Finless Porpoise (Cetacea) from the Arabian Sea and Bay of Bengal

Central Marine Fisheries Research Institute, PB No. 1603, Cochin 682 018, Kerala, India. 1Current address: Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar – 751 002, India
Central Marine Fisheries Research Institute, PB No. 1603, Cochin 682 018, Kerala, India
Central Marine Fisheries Research Institute, PB No. 1603, Cochin 682 018, Kerala, India
Madras Research Centre of CMFRI,75, Santhome High Road, Chennai 600 028
Central Marine Fisheries Research Institute, PB No. 1603, Cochin 682 018, Kerala, India
Central Marine Fisheries Research Institute, PB No. 1603, Cochin 682 018, Kerala, India
Marine Studies Section, Centre for Environment & Water-RI, King Fahd University of Petroleum & Minerals, Dhahran 31261, Saudi Arabia
AP 454, 22nd Street Thiruvalluvar Kudiyiruppu Anna Nagar, Chennai 600040, India
Central Marine Fisheries Research Institute, PB No. 1603, Cochin 682 018, Kerala, India
Export Inspection Agency, Marol, Andheri (East), Mumbai 400 093, India
Mammalia Delphinids finless porpoise mitochondrial DNA molecular taxonomy haplotypes

Abstract

The exact number of extant delphinid species from seas around India is still debated and the lack of adequate field keys and reliable inventory has resulted in misidentification of several species. As a part of a project to develop a molecular taxonomy of cetaceans from this region, partial sequences of mtDNA cytochrome b were generated from accidentally caught/stranded delphinids and finless porpoise. Species were identified by phylogenetic reconstruction of sample sequences with the reference sequences available in portals GenBank (NCBI) and the web-based program DNA Surveillance. A comparison was made with the homologous sequences of corresponding species from other seas of the world. Our molecular investigations allowed us to identify five species of cetaceans from Indian coasts, including Delphinus capensis, previously reported as D. delphis. We detected unique haplotypes in Indo pacific humpbacked dolphin (Sousa chinensis; n = 2) and finless porpoise (Neophocaena phocaenoides; n = 12) from Indian coast. On the other hand, some haplotypes were shared with other regional populations in spinner dolphin (Stenella longirostris; n = 16) and bottlenose dolphin (Tursiops aduncus; n = 3). Common dolphins (Delphinus capensis; n = 2) had both unique and shared haplotypes including one highly divergent sequence.

References

  1. Amaral, A.R., Sequeira, M., Cedeira, J.M. & Coelho, M.M. (2007) New insights on population genetic structure of Delphinus delphis from the northeast Atlantic and phylogenetic relationships within the genus inferred from two mitochondrial markers. Marine Biology, 151(5), 1967–1976.

    Arnason, U. & Gullberg, A. (1996) Cytochrome b nucleotide sequences and the identification of five primary lineages of extant cetaceans. Molecular Biology and Evolution, 13, 407–417.

    Baker C.S., Dalebout, M.L., Lavery, S. & Howard, A.R. (2003) www. DNA surveillance: applied molecular taxonomy for species conservation and discovery. TRENDS in Ecology and Evolution, 18(6), 271–272.

    Baker, C.S., Ross, H. Lavery, S., Rodrigo, A. & Dalebout, M.L. (2004) Progress on an applied molecular taxonomy of cetacean species. In: Reeves, R.R., Perrin, W.F., Taylor, B.L., Baker, C.S. & Mesnick, S.L. (Eds), Report of the Workshop on Shortcomings of Cetacean Taxonomy in Relation to Needs of Conservation and Management, La Jolla, California, pp 91.

    Caballero, S., Trujillo, F., Vianna, J.A., Barrios-Garrido, H., Montiel, M.G. Beltran-Pedreros, S., Marmontel, M., Santos, M.C., Rossi-Santos, M., Santos, F.R. & Baker, C.S. (2007) Taxonomic status of the genus Sotalia: species level ranking for 'tucuxi' (Sotalia fluviatilis) and 'costero' (Sotalia guianensis) dolphins. Marine Mammal Science, 23 (2), 358–386.

    Dalebout, M.L., Baker, C.S., Steel, D., Robertson, K.M., Chivers, S.J., Perrin, W.F., Mead, J.G., Grace, R.V. & Schofield, T.D. Jr. (2007) A divergent mtDNA lineage among Mesoplodon beaked whales: molecular evidence for a new species in the tropical Pacific?. Marine Mammal Science, 23(4): 954–966.

    Dizon, A., Baker, C.S., Cipriano, F., Lento, G., Palsboll, P. & Reeves, R. (2000) Molecular genetic identification of whales, dolphins, and porpoises: Proceedings of a workshop on the forensic use of molecular techniques to identify wildlife products in the market place. NOAA-TM-NMFS-SWFSC-286, 1–51.

    Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98.

    Hamilton, H., Caballero, S., Collins, A.G. & Brownell, R.L. Jr. (2001) Evolution of river dolphins. Proceedings of the Royal Society, London, B, Biological Sciences, 268 (1466), 549–556.

    Irwin, D.M., Kocher, T.D. & Wilson, A.C. (1991) Evolution of the Cytochrome b gene of mammals. Journal of Molecular Evolution, 32, 128–144.

    Jefferson, T.A. & Waerebeek, K.V. (2002) The taxonomic status of the nominal dolphin species Delphinus Tropicalis Van Bree, 1971. Marine Mammal Science, 18(4), 787–818.

    Kumaran, P.L. (2002) Marine mammal research in India – a review and critique of the methods. Current Science, 83(10), 1210–1220.

    Leduc, R.G., Perrin, W.F. & Dizon, A.E. (1999) Phylogenetic relationships among the delphinid cetaceans based on full cytochrome b sequences. Marine Mammal Science, 15(3), 619–648.

    Milinkovitch, M.C., Leduc, R., Tiedemann, R. & Dizon, A. (2002) Applications of molecular data in cetacean taxonomy and population genetics with special emphasis on defining species boundaries. In Evans, P.G.H. & Raga, J.A. (Eds) Marine Mammals: Biology and Conservation, Kluwer Academic/Plenum Publishers, London, UK, pp 325–359.

    Mirol, P.M., Mascheretti, S. & Searle, J.B. (2000) Multiple nuclear pseudogenes of mitochondrial cytochrome b in Ctenomys (Caviomorpha, Rodentia) with either great similarity to or high divergence from the true mitochondrial sequence. Heredity, 84(5), 538–547.

    Palumbi, S.R. (1996) Nucleic acids II: the Polymerase Chain Reaction. In: Hills, D. & Moritz, C. & Mable, B.K. (Eds.), Molecular Systematics. Sinaeur Associates, Sunderland, MA., pp 205–248.

    Perrin, W. F. (1990) Subspecies of Stenella longirostris (Mammalia: Cetacea, Delphinidae). Proceedings of the Biological Society, Washington, 103(2), 453–463.

    Perrin, W.F., Dolar, M.L.L. & Robineau, D. (1999) Spinner dolphins (Stenella longirostris) of the western Pacific and Southeast Asia: pelagic and shallow water forms. Marine Mammal Science, 15, 1029–1053.

    Perrin, W.F., Wursig, B. & Thewissen, J.G.M. (2002) Encyclopedia of marine mammals. Academic Press, San Diego, California.

    Reeves, R.R., Perrin, W.F., Taylor, B.L. Baker, C.S. & Mesnick, S.L. (2004) Report of the Workshop on Shortcomings of Cetacean Taxonomy in Relation to Needs of Conservation and Management. La Jolla, California, 91 pp.

    Rice, D.W. (1998) Marine mammals of the world. NOAA Technical Report NMFSSSRF-711, 15pp.

    Rosel, P.E., Dizon, A.E. & Heyning, J.E. (1994) Genetic analysis of sympatric morphotypes of common dolphins (genus Delphinus). Marine Biology, 119, 159–167.

    Rosel, P.E., Dizon, A.E. & Haygood, M.G. (1995b) Variability of the mitochondrial control region in populations of the harbour porpoise, Phocoena phocoena, on interoceanic and regional scales. Canadian Journal of Fisheries and Aquatic Sciences, 52, 1210–1219.

    Rosel, P.E, France, S.C., Wang, J.Y. & Kocher, T.D. (1999) Genetic structure of harbour porpoise Phocaena phocoena populations in the northwest Atlantic based on mitochondrial and nuclear markers. Molecular Ecology, 8, S41–S54.

    Ross, H.A., Lento, G.M., Dalebout, M.L., Goode, M., Ewing, G., Mclaren, P., Rodrigo, A.G., Lavery, S. & Baker, C.S. (2003) DNA Surveillance: Web-based molecular identification of whales, dolphins and porpoises. Journal of Heredity, 94(2), 111 – 114.

    Sambrook, E., Fritsch, F. & Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbour Press, Cold Spring Harbour, New York.

    Sathasivam, K. (2004) Marine Mammals of India, University Press, Hyderabad, xxiii, 184 pp.

    Shirakihara, M., Yoshida, H. & Shirakihara, K. (2003) Indo-Pacific bottlenose dolphins Tursiops aduncus in Amakusa, western Kyushu, Japan. Fisheries Science, 69, 654–656.

    Sutaria, D., & Jefferson, T.A. (2004) Records of Indo-Pacific Humpback dolphins (Sousa chinensis, Osbeck, 1765) Along the Coasts of India and Sri Lanka: An Overview. Aquatic mammals, 30(1), 125–136.

    Thompson, J.D., Higgins, D.G. & Gibson, T.J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weigh matrix choice. Nucleic Acids Research, 22, 4673–4680.

    Yousuf, K.S.S.M., Anoop, A.K., Anoop, B., Afsal, V.V., Vivekanandan, E., Kumarran, R.P., Rajagopalan, M., Krishnakumar, P.K., & Jayasankar, P. (2008) Observations on incidental catch of cetaceans in three landing centres along the Indian coast. Journal of Marine Biological Association UK 2 Biodiversity records, online publication (Ref no. 6254) pp.6.