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Type: Article
Published: 2003-04-09
Page range: 1–10
Abstract views: 34
PDF downloaded: 5

SEM and systematic studies of Steinernema abbasi Elawad et al., 1997, and S. riobrave Cabanillas et al., 1994 (Rhabditida: Steinernematidae)

Entomology and Nematology Department, University of Florida, Gainesville, FL 32611-0620, USA
Entomology and Nematology Department, University of Florida, Gainesville, FL 32611-0620, USA
Nematoda Entomopathogenic nematodes D2/D3 ITS morphology S. abbasi S. riobrave systematics

Abstract

SEM studies of the two nematodes Steinernema abbasi and S. riobrave revealed that they have two horn-like structures on the labial region similar to those of S. bicornutum, S. ceratophorum, S. pakistanense, and S. thermophilium. The structures were not mentioned in the original descriptions. In addition , S. riobrave has the twelfth pair of genital papillae at the edge of the cloaca. The papillae at this position were reported only in S. scapterisci. The formula of the lateral field pattern of S. abbasi is 2, 6, 8, 7, 6, 2, and that of S. riobrave is 2, 7, 8, 6, 2. Phylogenetic trees from sequences of D2/D3, and ITS regions of Steinernema species show that the four species S. abbasi, S. bicornutum, S. ceratophorum, and S. riobrave comprise a monophyletic group. These relationships support the present morphological studies.

References

  1. Adams, B.J., & Nguyen, K.B. (2002) Taxonomy and systematics. In: Gaugler, R. (ed) Entomopathogenic Nematology. CABI Publishing, Oxon, UK, pp. 1-33.

    Nguyen, K.B. & Smart, G.C. Jr. (1992) Addendum to the morphology of Steinernema scapterisci. Journal of Nematology, 24, 478-481.

    Nguyen, K.B. & Smart, G.C. Jr. (1995) Scanning electron microscope studies of Steinernema glaseri (Nematoda: Steinernematidae). Nematologica, 41, 183-190.

    Nguyen, K.B. & Smart, G.C. Jr. (1997) Scanning electron microscope studies of spicules and gubernacula of Steinernema spp. (Nemata: Steinernematidae). Nematologica, 43, 465-480.

    Nguyen, K.B., Maruniak, J. & Adams, B.J. (2001) The diagnostic and phylogenetic utility of the rDNA internal transcribed spacer sequences of Steinernema. Journal of Nematology, 33, 73-82.

    Stock, S.P., Campbell, J.F. & Nadler, S.A. (2001) Phylogeny of Steinernema Travassos, 1927 (Cephalobina: Steinernematidae) inferred from ribosomal DNA sequences and morphological characters. Journal of Parasitology, 87, 877-889.

    Swofford, D.L. (2001) PAUP*. Phylogenetic Analysis Using Parsimony. (*and other methods), vers 4.0b8. Sunderland, MA: Sinauer Associates.