Abstract
Wagnerinus frugivorus sp. nov. (Ceutorhynchinae: Ceutorhynchini) is described from Hokkaido, northern Japan, based on morphological and biological characters. Morphologically, this new species closely resembles Wagnerinus carinulatus (Faust) from the Russian Far East and W. costatus (Hustache) from Japan and Korea in having an emarginate anterior margin of the rostrum, sternite VIII diminished to a pair of small sclerites, and longitudinal rows of endophallic sclerites. However, it is distinctive enough to be distinguished from W. carinulatus and W. costatus mainly by the more conspicuous mucrones of mid and hind tibiae, deeper concavities of ventrites I and II, and larger paired prominences of ventrite V. Also, W. frugivorus clearly differs from W. costatus in terms of host plant utilization, the former feeds on the seed capsules of Weigela middendorffiana (Caprifoliaceae) as larvae, while the latter utilizes midge galls on the axillary buds of Weigela species. In addition to general taxonomic information, we provide a 1366-bp fragment of the mitochondrial cytochrome oxidase subunit I gene from the holotype as a DNA barcode of W. frugivorus and discuss the importance of DNA barcoding combined with species descriptions.References
Ball, S.L., Hebert, P.D.N., Burian, S.K. & Webb, J.M. (2005) Biological identifications of mayflies (Ephemeroptera) using DNA barcodes. Journal of the North American Benthological Society, 24, 508–524.
Blaxter, M. (2003) Counting angels with DNA. Nature, 421, 122–124.
Brower, A.V.Z. (2006) Problems with DNA barcodes for species delimitation: ‘ten species’ of Astraptes fulgerator reassessed (Lepidoptera: Hesperiidae). Systematics and Biodiversity, 4, 127–132.
Brown, J.W., Miller, S.E. & Horak, M. (2003) Studies on New Guinea moths. 2. Description of a new species of Xenothictis Meyrick (Lepidoptera: Tortricidae: Archipini). Proceedings of the Entomological Society Washington, 105, 1043–1050.
Burns, J.M., Janzen, D.H., Hajibabaei, M., Hallwachs, W. & Hebert, P.D.N. (2006) DNA barcodes of closely related (but morphologically and ecologically distinct) species of Skipper Butterflies (Hesperiidae) can differ by only one to three nucleotides. Journal of the Lepidopterists’ Society, 61, 138–153.
Cognato, A.I. (2006) Standard percent DNA sequence difference for insects does not predict species boundaries. Journal of Economic Entomology, 99, 1037–1045.
Colonnelli, E. (1984) Notes sur quelques Ceutorhynchinae de l’Afrique tropicale (Coleoptera, Curculionidae). Annales Historico-naturales Musei Nationalis Hungarici, 76, 208–238.
Colonnelli, E. (2004) Catalogue of Ceutorhynchinae of the World, with a Key to Genera (Insecta: Coleoptera: Curculionidae). Argania editio, Barcelona.
Cruickshank, R.H., Johnson, K.P., Smith, V.S., Adams, R.J., Clayton, D.H. & Page, R.D.M. (2001) Phylogenetic analysis of partial sequences of elongation factor 1α identifies major groups of lice (Insecta: Phthiraptera). Molecular Phylogenetics and Evolution, 19, 202–215.
Dasmahapatra, K.K. & Mallet, J. (2006) DNA barcodes: recent successes and future prospects. Heredity, 97, 254–55.
DeSalle, R. (2006) Species discovery versus species identification in DNA barcoding efforts: response to Rubinoff. Conservation Biology, 20, 1545–1547.
Ebach, M.C. & Holdrege, C. (2005a) DNA barcoding is no substitute for taxonomy. Nature, 434, 697.
Ebach, M.C. & Holdrege, C. (2005b) More taxonomy, not DNA barcoding. BioScience, 55, 823–824.
Faust, J. (1887) Curculioniden aus dem Amur-Gebiet. Deutsche entomologische Zeitschrift, 31, 161–180.
Gilbert, M.T.P., Moore, W., Melchior, L. & Worobey, M. (2007) DNA extraction from dry museum beetles without conferring external morphological damage. PLoS ONE 2(3), e272. doi:10.1371/journal.pone.0000272
Greenstone, M.H., Rowley, D.L., Heimbach, U., Lundgren, J.G., Pfannenstiel, R.S. & Rehner, S.A. (2005) Barcoding generalist predators by polymerase chain reaction: carabids and spiders. Molecular Ecology, 14, 3247–3266.
Grimaldi, D. & Engel, M.S. (2005) Evolution of the Insects, Cambridge University Press, New York.
Gompert, Z., Nice, C.C., Fordyce J.A., Forister M.L. & Shapiro A.M. (2006) Identifying units for conservation using molecular systematics: the cautionary tale of the Karner blue butterfly. Molecular Ecology, 15, 1759–1768.
Hajibabaei, M., Janzen, D.H., Burns, J.M., Hallwachs, W. & Hebert, P.D.N. (2006a) DNA barcodes distinguish species of tropical Lepidoptera. Proceedings of the National Academy of Sciences of the United States of America, 103, 968–971.
Hajibabaei, M., Singer, G.A.C. & Hickey, D.A. (2006b) Benchmarking DNA barcodes: an assessment using available primate sequences. Genome, 49, 851–854.
Hajibabaei, M., Smith, M.S., Janzen, F.H., Rodriguez, J.J., Whitfield, J.B. & Hebert, P.D.N. (2006c) A minimalist barcode can identify a specimen whose DNA is degraded. Molecular Ecology Notes, 6, 959–964.
Hebert, P.D.N., Cywinska, A., Ball, S.L. & DeWaard, J.R. (2003a) Biological identifications through DNA barcodes. Proceedings of the Royal Society London B, 270, 313–321.
Hebert, P.D.N., Ratnasingham, S. & deWaard, J.R. (2003b) Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proceedings of the Royal Society London B, 270 (Supplement), S96–S99.
Hebert, P.D.N., Penton, E.H., Burns, J.M., Janzen, D.H. & Hallwachs, W. (2004) Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator. Proceedings of the National Academy of Sciences of the United States of America, 101, 14812–14817.
Hustache, A. (1916) Synopsis des Ceuthorrhynchini du Japon. Annales de la Société Entomologique de France, 85, 107–144.
Hurst, G.D.D. & Jiggins, F.M. (2005) Problems with mitochondrial DNA as a marker in population, phylogeographic and phylogenetic studies: the effects of inherited symbionts. Proceedings of the Royal Society London B, 272, 1525–1534.
Janzen, D.H. (2004) Now is the time. Philosophical Transactions of the Royal Society B: Biological Sciences, 359, 731–732.
Kato, T., Yoshitake, H. & Ito, M. (2006) Phylogenetic position of the genus Wagnerinus Korotyaev (Coleoptera: Curculionidae) associated with galls induced by Asphondylia baca Monzen (Diptera: Cecidomyiidae). In: Ozaki, K., Yukawa, J., Ohgushi, T. & Price, P. W. (Eds), Galling Arthropods and Their Associates: Ecology and Evolution. Springer-Verlag, Tokyo, pp. 297–305.
Knölke, S., Erlacher, S., Hausmann, A., Miller, M.A. & Segerer, A.H. (2005) A procedure for combined genitalia dissection and DNA extraction in Lepidoptera. Insect Systematics and Evolution, 35, 401–409.
Korotyaev, B.A. (1980) Materials to the knowledge of Ceutorhynchinae (Coleoptera, Curculionidae) of Mongolia and the USSR. Nasekomie Mongolii, 7, 107–282. (In Russian)
Korotyaev, B.A. (1981) New and little-known weevils of the subfamily Ceutorhynchinae (Coleoptera, Curculionidae) from the Palearctic, Indo-Malayan, and Australian regions. Entomologicheskoe obozrenie, 60, 126–159. (In Russian)
Meier, R., Shiyang, K., Vaidya, G. & Ng, P. K. L (2006) DNA barcoding and taxonomy in Diptera: a tale of high intraspecific variability and low identification success. Systematic Biology, 55, 715–728.
Monaghan, M.T., Balke, M., Gregory T.R. & Vogler, A.P. (2005) DNA-based species delineation in tropical beetles using mitochondrial and nuclear markers. Philosophical Transactions of the Royal Society B, 360, 1925–1933.
Monzen, K. (1937) On some new gall midges. Kontyu, 11, 180–194. (In Japanese)
Moritz, C. & Cicero, C. (2004) DNA barcoding: promise and pitfalls. PLoS Biology, 2, 1529–1531.
Ohba, H. (1993) Caprifoliaceae. In: Iwatsuki, K., Boufford, D. E. & Ohba, H. (Eds.), Flora of Japan IIIa. Kodansha, Tokyo, pp. 420–448.
Rubinoff, D. (2006) Utility of mitochondrial DNA barcodes in species conservation. Conservation Biology, 20, 1026–1033.
Savolainen, V., Cowan, R.S., Vogler, A.P., Roderick, G.K. & Lane, R. (2005) Towards writing the encyclopaedia of life: an introduction to DNA barcoding. Philosophical Transactions of the Royal Society B, 360, 1805–1811.
Scheffer, S.J., Lewis, M.L. & Joshi, R.C. (2006) DNA barcoding applied to invasive leafminers (Diptera: Agromyzidae) in the Philippines. Annals of the Entomological Society of America, 99, 204–210.
Schindel, D.E. & Miller, S.E. (2005) DNA barcoding a useful tool for taxonomists. Nature, 435, 17.
Simon, C., Frati, F., Beckenbach, A., Crespi, B., Liu, H. & Flook, P. (1994) Evolution, weighting and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Annals of the Entomological Society of America, 87, 651-701.
Smith, M.A., Woodley, N.E., Janzen, D.H., Hallwachs, W. & Hebert, P.D.N. (2006) DNA barcodes reveal cryptic host-specificity within the presumed polyphagous members of a genus of parasitoid flies (Diptera: Tachinidae). Proceedings of the National Academy of Sciences of the United States of America, 103, 3657–3662.
Sperling, F. (2003) DNA barcoding: Deus ex Machina. Newsletter of the Biological Survey of Canada (Terrestrial Arthropods), Opinion Page, 22, 50–53.
Sugiura, S., Yamazaki, K. & Fukasawa, Y. (2004) Weevil parasitism of ambrosia galls. Annals of the Entomological Society of America, 97, 184–193.
Tuda, M., Fukatsu, T. & Shimada, M. (1995) Species differentiation of bruchid beetles (Coleoptera: Bruchidae) analyzed by mitochondrial DNA polymorphism. Applied Entomology and Zoology, 30, 377-380.
Tuda, M., Wasano, N., Kondo, N., Horng, S-B., Chou, L-Y. & Tateishi, Y. (2004) Habitat-related mtDNA polymorphism in the stored-bean pest Callosobruchus chinensis (Coleoptera: Bruchidae). Bulletin of Entomological Research, 94, 75-80.
Uechi, N., Yukawa, J. & Yamaguchi, D. (2004) Host alternation by gall midges of the genus Asphondylia (Diptera: Cecidomyiidae). Bishop Museum Bulletin in Entomology, 12, 53–66.
Yonekura, K. & Kajita, T. (2003) BG Plants. Available from: http://bean.bio.chiba-u.jp/bgplants/ylist_main.html (10 January 2007).
Will, K.W. & Rubinoff, D. (2004) Myth of the molecule: DNA barcodes for species cannot replace morphology for identification and classification. Cladistics, 20, 47–55.
Will, K.W., Mishler, B.D. & Wheeler, Q.D. (2005) The perils of DNA barcoding and the need for integrative taxonomy. Systematic Biology, 54, 844–851.