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Type: Article
Published: 2026-02-23
Page range: 265-285
Abstract views: 26
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A review of Rheosmittia Brundin, 1986 (Diptera: Chironomidae) from China based on DNA barcodes and morphology

Engineering Research Center of Environmental DNA and Ecological Water Health Assessment; Shanghai Ocean University; Shanghai 201306; China. Shanghai Universities Key Laboratory of Marine Animal Taxonomy and Evolution; Shanghai Ocean University; Shanghai 201306; China.
Engineering Research Center of Environmental DNA and Ecological Water Health Assessment; Shanghai Ocean University; Shanghai 201306; China. Shanghai Universities Key Laboratory of Marine Animal Taxonomy and Evolution; Shanghai Ocean University; Shanghai 201306; China.
Engineering Research Center of Environmental DNA and Ecological Water Health Assessment; Shanghai Ocean University; Shanghai 201306; China. Shanghai Universities Key Laboratory of Marine Animal Taxonomy and Evolution; Shanghai Ocean University; Shanghai 201306; China.
Engineering Research Center of Environmental DNA and Ecological Water Health Assessment; Shanghai Ocean University; Shanghai 201306; China. Shanghai Universities Key Laboratory of Marine Animal Taxonomy and Evolution; Shanghai Ocean University; Shanghai 201306; China.
Engineering Research Center of Environmental DNA and Ecological Water Health Assessment; Shanghai Ocean University; Shanghai 201306; China. Shanghai Universities Key Laboratory of Marine Animal Taxonomy and Evolution; Shanghai Ocean University; Shanghai 201306; China.
Diptera Orthocladiinae taxonomy female larva key

Abstract

Rheosmittia Brundin, 1986 (Diptera: Chironomidae) from China is reviewed based on DNA barcodes and morphology. Two new species, Rheosmittia motuoensis Zhang & Lin, sp. nov. from Xizang and Rheosmittia wangi Zhang & Lin, sp. nov. from Yunnan, as well as an unidentified larva from Zhejiang, are described and illustrated. The first new species is characterized by its distinctive body coloration, smaller AR, and inferior volsella positioned at the midlength of the gonocoxite, while the second new species is distinguished by slightly curved gonostylus and longer costa extension. Given the distinctive morphology of the gonostylus in R. wangi within the genus, generic diagnosis is amended. A key to adult males of extant Rheosmittia is presented.

 

References

  1. Andersen, T., Sæther, O., Cranston, P. & Epler, J. (2013) The larvae of Orthocladiinae (Diptera: Chironomidae) of the Holarctic region–Keys and diagnoses. Insect Systematics & Evolution, 66, 189–385.
  2. Brundin, L. (1956) Zur Systematik der Orthocladiinae (Dipt., Chironomidae). Report of the Institute of Freshwater Research, Drottningholm. Vol. 37. Fishery Board of Sweden, Lund, 181 pp.
  3. Caldwell, B.A. (1996) Two new Nearctic species of small Orthocladiinae (Diptera: Chironomidae) with notes on ecology. Hydrobiologia, 328, 1–7. https://doi.org/10.1007/BF00016897
  4. Caspers, N. & Reiss, F. (1989) Die Chironomidae der Türkei. Teil I: Podonominae, Diamesinae, Prodiamesinae, Orthocladiinae (Diptera, Nematocera, Chironomidae). Entomofauna, Zeitschrift Für Entomologie, 10, 105–160.
  5. Cranston, P. & Sæther, O. (1986) Rheosmittia (Diptera: Chironomidae): a generic validation and revision of the western Palaearctic species. Journal of Natural History, 20, 31–51. https://doi.org/10.1080/00222938600770041
  6. Cranston, P., Oliver, D. & Sæther, O. (1989) The adult males of Orthocladiinae (Diptera: Chironomidae) of the Holarctic region—Keys and diagnoses. Entomologica Scandinavica Supplement, 34, 165–352.
  7. Cranston, P.S. & Tang, H. (2024) An identification guide to the genera of aquatic larval Chironomidae (Diptera) of south-east Asia. Zootaxa, 5497 (2), 151–193. https://doi.org/10.11646/zootaxa.5497.2.1
  8. de Waard, J., Ivanova, N., Hajibabaei, M. & Hebert, P. (2008) Assembling DNA barcodes: analytical protocols. In: Cristofre, M. (Ed.), Methods in Molecular Biology: Environmental Genetics. Humana Press Inc., Ottawa, pp. 275–293. https://doi.org/10.1007/978-1-59745-548-0_15
  9. Doitteau, G. & Nel, A. (2007) Chironomid midges from early Eocene amber of France (Diptera: Chironomidae). Zootaxa, 1404 (1), 1–66. https://doi.org/10.11646/zootaxa.1404.1.1
  10. Edgar, R.C. (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research, 32, 1792–1797. https://doi.org/10.1093/nar/gkh340
  11. Fasbender, A., Spring, R. & Hayford, B. (2025) Description of Parakiefferiella ferringtoni with discussion of relationships within the Parakiefferiella group (Diptera, Chironomidae, Orthocladiinae). CHIRONOMUS Journal of Chironomidae Research, 40, 73–90. https://doi.org/10.5324/cjcr.v0i40.6300
  12. Lin, X.-L., Mo, L.-D., Bu, W.-J. & Wang, X.-H. (2021) The first comprehensive DNA barcode reference library of Chinese Tanytarsus (Diptera: Chironomidae) for environmental DNA metabarcoding. Diversity and Distributions, 27, 1932–1941. https://doi.org/10.1111/ddi.13209
  13. Lin, X., Stur, E. & Ekrem, T. (2015) Exploring genetic divergence in a species-rich insect genus using 2790 DNA barcodes. PLoS ONE, 10, e0138993. https://doi.org/10.1371/journal.pone.0138993
  14. Orendt, C. & Bendt, T. (2021) Orthocladiinae sensu lato (Orthocladiinae, Prodiamesinae, Diamesinae, Podonominae, Buchonomyiinae, Telmatogetoninae) (Diptera: Chironomidae). Keys to Central European larvae with respect to macroscopic characters. Deutsche Gesellschaft für Limnologie eV, Essen, 144 pp.
  15. Ratnasingham, S. & Hebert, P.D. (2007) bold: The Barcode of Life Data System (http://www.barcodinglife.org). Molecular Ecology Notes, 7, 355–364. https://doi.org/10.1111/j.1471-8286.2007.01678.x
  16. Sæther, O. (2006) Japanese Pseudosmittia Edwards (Diptera: Chironomidae). Zootaxa, 1198 (1), 21–51. https://doi.org/10.11646/zootaxa.1198.1.2
  17. Sæther, O.A. (1969) Some Nearctic Podonominae, Diamesinae, and Orthocladiinae (Diptera: Chironomidae). Bulletin of the Fisheries Research Board of Canada, 170, 1–154.
  18. Sæther, O.A. (1980) Glossary of chironomid morphology terminology (Diptera: Chironomidae). Entomologica Scandinavica Supplement, 14, 1–51.
  19. Sasa, M. & Suzuki, H. (2000) Studies on the chironomid midges collected on Yakushima Island, Southwestern Japan. Tropical medicine, 42, 53–134.
  20. Song, C., Lin, X.L., Wang, Q. & Wang, X.H. (2018) DNA barcodes successfully delimit morphospecies in a superdiverse insect genus. Zoologica Scripta, 47, 311–324. https://doi.org/10.1111/zsc.12284
  21. Stasiukynas, L., Havelka, J., da Silva, F.L., Torres Jimenez, M.F., Podënas, S. & Lekoveckaitë, A. (2025) COI Insights into Diversity and Species Delimitation of Immature Stages of Non-Biting Midges (Diptera: Chironomidae). Insects, 16, 174. https://doi.org/10.3390/insects16020174
  22. Tamura, K., Stecher, G. & Kumar, S. (2021) MEGA11: molecular evolutionary genetics analysis version 11. Molecular Biology and Evolution, 38, 3022–3027. https://doi.org/10.1093/molbev/msab120
  23. Wang, H., Xu, H.-F., Li, C.-H., Zhang, H.-X., Zhang, Y.-P., Sun, B.-J. & Lin, X.-L. (2024) Towards a Comprehensive DNA Barcode Library of Stenochironomus Kieffer, 1919 (Diptera: Chironomidae) from China. Diversity, 16, 257. https://doi.org/10.3390/d16050257
  24. Xu, H.-F., Xiao, X.-R., Zhang, Z.-C., Li, Y.-F. & Lin, X.-L. (2025) Mitogenomic Insights into Orthocladiinae (Diptera: Chironomidae): Structural Diversity and Phylogenetic Implications. Biology, 14, 1178. https://doi.org/10.3390/biology14091178
  25. Yao, Y., Chen, J.-Y., Gong, X.-L., Li, C.-H., Liu, Z. & Lin, X.-L. (2025) Species Delimitation and Cryptic Diversity in Rheotanytarsus Thienemann & Bause, 1913 (Diptera: Chironomidae) Based on DNA Barcoding. Insects, 16, 370. https://doi.org/10.3390/insects16040370

How to Cite

Zhang, Z.-C., Chen, Y.-Z., Zhang, Y., Yao, Y. & Lin, X.-L. (2026) A review of Rheosmittia Brundin, 1986 (Diptera: Chironomidae) from China based on DNA barcodes and morphology. Zootaxa, 5760 (2), 265–285. https://doi.org/10.11646/zootaxa.5760.2.6