Abstract
Four new fossil species from two families of Acalyptratae (Diptera), all preserved in Baltic amber (Eocene, 48–34 Ma), are described: Acartophthalmites bicolor sp. nov., A. scotopteryx sp. nov., A. striatus sp. nov. (all Clusiomitidae), as well as Protanthomyza chelicerata sp. nov. (Protanthomyzidae). All new species are diagnosed, illustrated, and their relationships are discussed. Updated identification keys are provided. Based on newly acquired morphological data, the relationships of the subfamily Protanthomyzinae (formerly within Anthomyzidae) are revised. Since this fossil taxon is recognized as the sister group of Anthomyzidae (s. str.) (= without Protanthomyzinae) +Opomyzidae,, this group is elevated to the rank of a family, Protanthomyzidae stat. nov. The addition of four new species contributes to the knowledge of the diversity of Acalyptratae flies in the Eocene Baltic amber forest, even though they belong to extinct families.
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