Abstract
Nymphs of the genus Ephemera are usually found in sandy/muddy habitats but have not been collected from purely muddy sediments in China. However, a tentative sampling in a small wetland surrounded by agricultural fields revealed a new species Ephemera aureola sp. nov. The reared adults and collected nymphs demonstrate that this species exhibits mixed characters of the subgenera Ephemera and Sinephemera. Remarkably, the new species possesses straight stripes on abdominal terga VI–X, while its sterna have oblique streaks. The male genitalia have titillators, and the wings are almost uniformly golden. The nymphs have a shallow median ridge and setal tuft on tergum I, while its frons has a shallowly concave anterior margin. There is only one diagnostic feature separating subgenus Ephemera.
References
Bae Y. J. 1995. Ephemera separigata, a new species of Ephemeridae (Insecta: Ephemeroptera) from Korea. Korean Journal of Systematic Zoology 11(2): 159–166.
Bauernfeind E. & Soldán T. 2012. The Mayflies of Europe (Ephemeroptera). Apollo Books, Ollerup, 781 pp. https://doi.org/10.14411/eje.2013.036
Blouin M. A., Hudson P. & Chriscinske M. 2004. Habitat selection by two species of burrowing mayfly nymphs in the Les Cheneaux Islands region of northern Lake Huron. Journal of Freshwater Ecology 19(3): 507–514. https://doi.org/10.1080/02705060.2004.9664926
Eaton A. E. 1871. A monograph on the Ephemeridae. Transactions of the Entomological Society of London 1–6: 1–164. https://doi.org/10.1111/j.1365-2311.1871.tb01484.x
Fairchild G. W., Faulds A. M. & Saunders L. L. 1999. Pp. 423–446. In: D. P. Batzer, R. B. Rader and S. A. Wissinger (eds.): Constructed marshes in southeast Pennsylvania: invertebrate food web structure. Invertebrates in Freshwater Wetlands of North America: Ecology and Management. John Wiley & Sons, Inc. New York, NY, USA.
Fischer S., Cristina Marinone M., Soledad Fontanarrosa M., Nieves M. & Schweigmann N. 2000. Urban rain pools: seasonal dynamics and entomofauna in a park of Buenos Aires. Hydrobiologia 441: 45–53.
Folmer O., Black M., Hoeh W., Lutz R. & Vrijenhoek R. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3(5): 294–299.
Hrivniak Ľ. U. B. O. Š., Sroka P., Godunko R. J. & Žurovcová M. 2017. Mayflies of the genus Epeorus Eaton, 1881 sl (Ephemeroptera: Heptageniidae) from the Caucasus Mountains: a new species of Caucasiron Kluge, 1997 from Georgia and Turkey. Zootaxa 4341 (3): 353–374.
Hsu Y.–C. 1937. The mayflies of China. Peking Natural history Bulletin 11(4): 433–440.
Humphreys R. K. & Ruxton G. D. 2018. A review of thanatosis (death feigning) as an anti-predator behaviour. Behavioral Ecology and Sociobiology 72: 1–16. https://doi.org/10.1007/s00265-017-2436-8
Hwang J. M., Bae Y. J. & McCafferty W. 2008. A checklist of the Ephemeridae (Ephemeroptera). pp. 58–172, In: F. R. Hauer and A. Welch (eds.): International Advances in the Ecology, Zoogeography, and Systematics of Mayflies and Stoneflies. University of California Press, Berkeley, California. https://doi.org/10.1525/california/9780520098688.003.0012
Jacobus L. M., Macadam C. R. & Sartori M. 2019. Mayflies (Ephemeroptera) and their contributions to ecosystem services. Insects 10(170): 1–26. https://doi.org/10.3390/insects10060170
Johnson B. R., Tarter D. C. & Hutchens J. J. 2000. Life history and trophic basis of production of the mayfly Callibaetis fluctuans (Walsh) (Ephemeroptera: Baetidae) in a mitigated wetland, West Virginia, USA. Wetlands 20(2): 397–405.
Kang S.-C. & Yang C.-T. 1995. Ephemerellidae of Taiwan (Insecta, Ephemeroptera). Bulletin of National Museum of Natural Science 5: 95–116.
Kimura M. 1980. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16: 111–120. https://doi.org/10.1007/bf01731581
Kluge N. J. 2004. The Phylogenetic System of Ephemeroptera. Kluwer Academic Publishers. 456 pp.
Lei Z.-M. & Zhou C.-F. 2024. The subgenus Sinephemera Kluge, 2004 in China (Ephemeroptera: Ephemeridae: Ephemera Linnaeus, 1758). Zootaxa 5517(1): 1–68. https://doi.org/10.11646/zootaxa.5517.1.1
Lei Z.-M., Li M.-Y., Deng M.-H. & Zhou C.-F. 2024. First description of the Chinese mayfly Ephemera lota Navás, 1934 (Ephemeroptera: Ephemeridae) based on imagines with designation of the species neotype. Aquatic Insects 45(3): 319–334. https://doi.org/10.1080/01650424.2023.2291385
Li M.-Y., Deng M.-H. & Zhou C.-F. 2023. The exact morphology of the species Ephemera pieli Navás, 1934 from Eastern China (Ephemeroptera: Ephemeridae). Zootaxa 5271(2): 345–354. http://dx.doi.org/10.11646/zootaxa.5271.2.8
Linnaeus C. 1758. Systema Naturae per regna tria Naturae, secundum classes, ordines, genera, species, cum Characteribus, Differentiis, Synonymis, Locis. Vol. 1. 10th Edition. Impensis Direct. Laurentii Salvii, holmiae, 824 pp. https://doi.org/10.5962/bhl.title.542
Matsumura S. 1911. Erster Beitrag zur Insecten-Fauna von Sachalin. Journal of the College of Agriculture (Tohoku Imperial University, Sapporo) 4(1): 1–145.
McCafferty W. P. 1975. The burrowing mayflies (Ephemeroptera: Ephemeroidea) of the United States. Transactions of the American Entomological Society 101: 447–504.
McLachlan R. 1894. XLVI.—On two small collections of neuroptera from Tachien-lu, in the province of Szechuen, Western China, on the frontier of Tibet. Journal of Natural History 13(77): 421–436. https://doi.org/10.1080/00222939408677723
Navás L. 1917. Nevropteres de l'Indo-Chine (2e serie). Revue illustree d'Entomologie (Rennes) 7: 8–17.
Navás L. 1934. Névroptères et insectes voisins. Chine et pays environnants. 6e Série. Notes d'Entomologie Chinoise (Musée Heude) 1(14): 1–10.
Navás L. 1935. Décadas de insectos nuevos. Década 27. Brotéria (Ciências Naturais) 4(3): 97–107.
Pictet F. J. 1843–1845. Histoire naturelle generale et particuliere des insectes neuropteres. Famille des ephemerines. Chez J. Kessmann et Ab. Cherbuliz, geneva, 300 pp. https://doi.org/10.5962/bhl.title.48625
Sun J. Z. & Zhou C.-F. 2016. The nymph, habitat, and status of Eatonigenia in China (Ephemeroptera: Ephemeridae). Zootaxa 4193(2): 381–389. https://doi.org/10.11646/zootaxa.4193.2.12
Tamura K., Stecher G. & Kumar S. 2021. MEGA 11: Molecular Evolutionary Genetics Analysis Version 11. Molecular Biology and Evolution 38(7): 3022–3027. https://doi.org/10.1093/molbev/msab120
Ulmer G. 1924. Einige alte und neue Ephemeropteren. Konowia 3(1–2): 23–37.
Wakimura K., Takemon Y., Ishiwata S.-I., Tanida K., Abbas E. M., Inai K. & Kato M. 2020. A reference collection of Japanese aquatic macroinvertebrates. Ecological Genetics and Genomics 17: 100065. https://doi.org/10.1016/j.egg.2020.100065
Walsh B. D. 1863. Observations on certain N.A. Neuroptera, by H. Hagen, M.D., of Koenigsberg, Prussia; translated from the original French ms., and published by permission of the author, with notes and descriptions of about twenty new N.A. sp. of Pseudoneuroptera. Proceedings of the Entomological Society of Philadelphia 2: 167–272.
Wang L., Li B., Jiang J. & Tong X.-L. 2022. The complete mitochondrial genome of Ephemera serica (Ephemeroptera: Ephemeridae) and phylogenetic analysis. Mitochondrial DNA (Part B) 7(3): 461–463. https://doi.org/10.1080/23802359.2022.2044401
You D.-S. & Gui H. 1995. Economic Insect Fauna of China. Fasc. 48. Ephemeroptera. Beijing: Science Press, 152 pp. (In Chinese)