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Issue: Vol. 4941 No. 2: 8 Mar. 2021
Type: Article
Published: 2021-03-08
DOI: 10.11646/zootaxa.4941.2.5
Page range: 239–258
Abstract views: 766
PDF downloaded: 17

Description of a new species of Bush frog (Anura: Rhacophoridae: Raorchestes) from northwestern Yunnan, China

State Key Laboratory of Genetic Resources and Evolution State, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China Kunming College of Life Science, University of the Chinese Academy of Sciences, Kunming 650204, China
Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming, China
State Key Laboratory of Genetic Resources and Evolution State, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China Kunming College of Life Science, University of the Chinese Academy of Sciences, Kunming 650204, China
Zhejiang Forest Resource Monitoring Center, Hangzhou 310020
Gongshan Administrative suboffice of Gaoligongshan National Nature Reserve
State Key Laboratory of Genetic Resources and Evolution State, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China
Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming, China
State Key Laboratory of Genetic Resources and Evolution State, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China
Amphibia Rhacophoridae Raorchestes northwestern Yunnan new species Raorchestes dulongensis sp. nov.

Abstract

Approximately half of the species in speciose genus Raorchestes were described during the past 10 years, yet only 11 species are known from Southeast Asia and southern China (SEA-SC), adjacent Himalayas, and northeastern India. Field work in northwestern Yunnan province, China resulted in the discovery of one new species in the genus based on morphological and molecular analyses. The new species is diagnosed by small size with 15.0–19.0 mm SVL in adult males (n=3); tongue pyriform, notched posteriorly; rudimentary webbing between toes; fingers and toes with narrow lateral dermal fringes; tibiotarsal articulation reaching anterior of the eye when hindlimb is stretched along the side of the body; relative finger lengths: I < II < IV < III, relative toe lengths: I < II < V < III < IV; inner metatarsal tubercle oval, outer metatarsal tubercle absent; finger discs and toe discs greyish or orange; flank near the crotch with a distinct black region between two creamy white patches, and the thigh having a similar black patch near the groin, proximal to another creamy white patch; a distinct “) (”-shaped dark marking on the back; male with external single subgular vocal sac; nuptial pad absent. A phylogenetic tree was reconstructed based on the mitochondrial genes for 16S rRNA and ND1. The results indicated that these individuals form a monophyletic group, and show high genetic divergence to their closest relatives within the genus (uncorrected p-distances > 3.2%) by distance of 16S comparable to the divergence between recognized Raorchestes species. This study further enriches the diversity of rhacophorids, especially in northwestern Yunnan.

 

References

  1. Abraham, R.K., Pyron, R.A., Ansil, B.R., Zachariah, A. & Zachariah, A. (2013) Two novel genera and one new species of treefrog (Anura: Rhacophoridae) highlight cryptic diversity in the Western Ghats of India. Zootaxa, 3640 (2), 177–189.

    https://doi.org/10.11646/zootaxa.3640.2.3

    Al-Razi, H., Maria, M. & Muzaffar, S.B. (2020) A new species of cryptic Bush frog (Anura, Rhacophoridae, Raorchestes) from northeastern Bangladesh. Zookeys, 927, 127–151.

    https://doi.org/10.3897/zookeys.927.48733

    Anderson, J. (1878) Reptilia and amphibia[M]//Anderson J. Anatomical and Zoological Researches: Comprising an Account of the Zoological Results of the Two Expeditions to Western Yunnan in 1868 and 1875. Vol 1. Bernard Quaritch. London, pp. 703–860 + 969–975 (index).

    https://doi.org/10.5962/bhl.title.50434

    Annandale, N. (1912) Zoological results of the Abor Expedition, 1911–1912. I. Amphibia. Records of the Indian Museum, 8, 7–36.

    https://doi.org/10.5962/bhl.part.1186

    Biju, S.D. & Bossuyt, F. (2009) Systematics and phylogeny of Philautus. Gistel, 1848 (Anura, Rhacophoridae) in the Western Ghats of India, with descriptions of 12 new species. Zoological Journal of the Linnean Society, 155 (2), 374–444.

    https://doi.org/10.1111/j.1096-3642.2008.00466.x

    Biju, S.D., Shouche, Y., Dubois, A., Dutta, S.K. & Bossuyt, F. (2010) A ground-dwelling rhacophorid frog from the highest mountain peak of the Western Ghats of India. Current Science, 98, 1119–1125.

    Bossuyt, F. & Dubois, A. (2001) A review of the frog genus Philautus Gistel, 1848 (Amphibia, Anura, Ranidae, Rhacophorinae). Zeylanica, 6 (1), 1–112.

    Boulenger, G.A. (1906) Description of two Indian frogs. Journal & Proceedings of Asiatic Society of Bengal, New Series, 2 (9), 385–386.

    Boulenger, G.A. (1919) Descriptions of three new batrachians from the Garo Hills, Assam. Records of the Indian Museum, 16, 207–208.

    https://doi.org/10.5962/bhl.part.25921

    Chan, K.O., Grismer, L.L. & Brown, R.M. (2018) Comprehensive multi-locus phylogeny of Old World tree frogs (Anura: Rhacophoridae) reveals taxonomic uncertainties and potential cases of over-and underestimation of species diversity. Molecular Phylogenetics and Evolution, 127, 1010–1019.

    https://doi.org/10.1016/j.ympev.2018.07.005

    Che, J., Jiang, K., Yan, F. & Zhang, Y.P. (2020) Amphibians and Reptiles in Tibet—Diversity and Evolution. Science Press, Beijing, 353pp.

    Chen, J.M., Prendini, E., Wu, Y.H., Zhang, B.L., Suwannapoom, C., Chen, H.M., Jin, J.Q., Lemmon, E.M., Lemmon, A.R., Stuart, B.L., Raxworthy, C.J., Murphy, R.W., Yuan, Z.Y. & Che, J. (2020) An integrative phylogenomic approach illuminates the evolutionary history of Old World tree frogs (Anura: Rhacophoridae). Molecular Phylogenetics and Evolution, 145, 106724.

    https://doi.org/10.1016/j.ympev.2019.106724

    Chen, Z., Pan, X., Xiao, H. & Yang, J. (2012) A new cyprinid species, Placocheilus dulongensis, from the upper Irrawaddy system in northwestern Yunnan, China. Zoologischer Anzeiger-A Journal of Comparative Zoology, 251 (3), 215–222.

    https://doi.org/10.1016/j.jcz.2011.09.002

    Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature Methods, 9 (8), 772.

    https://doi.org/10.1038/nmeth.2109

    Edgar, R.C. (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research, 32 (5), 1792–1797.

    https://doi.org/10.1093/nar/gkh340

    Fei, L., Hu, S.Q., Ye, C. & Huang, Y.Z. (2009) Fauna Sinica. Amphibia. Vol. Anura. Science Press, Beijing, 957 pp.

    Fei, L., Ye, C.Y. & Jiang, J.P. (2012) Colored Atlas of Chinese Amphibians and Their Distributions. Sichuan Publishing House of Science & Technology, Chengdu, 340 pp.

    Fouquet, A., Gilles, A., Vences, M., Marty, C., Blanc, M. & Gemmell, N.J. (2007) Underestimation of species richness in Neotropical frogs revealed by mtDNA analyses. PLoS One, 2 (10), e1109.

    https://doi.org/10.1371/journal.pone.0001109

    Frost, D.R. (2020) Amphibian species of the world: an Online Reference. Version 6.0. American Museum of Natural History, New York. Electronic Database accessible: http://research.amnh.org/herpetology/amphibia/index.html (accessed 14 July 2020)

    Jiang, D., Jiang, K., Ren, J., Wu, J. & Li, J. (2019) Resurrection of the genus Leptomantis, with description of a new genus to the family Rhacophoridae (Amphibia: Anura). Asian Herpetological Research, 10 (1), 1–12.

    Jiang, K., Yan, F., Wang, K., Zou, D.H., Cheng, L.I. & Che, J. (2016) A new genus and species of treefrog from Medog, southeastern Tibet, China (Anura, Rhacophoridae). Zoological Research, 37 (1), 15–20.

    Kocher, T.D., Thomas, W.K., Meyer, A., Edwards, S.V., Pääbo, S., Villablanca, F.X. & Wilson, A.C. (1989) Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers. Proceedings of the National Academy of Sciences of the USA, 86 (16), 6196–6200.

    https://doi.org/10.1073/pnas.86.16.6196

    Kou, Z.T. (1990) A new species of genus Philautus (Amphibia: Rhacophoridae) from Yunnan, China. In: From Water onto Land. China Forestry Press, Beijing, pp. 210–212.

    Kuramoto, M. & Joshy, S.H. (2003) Two new species of the genus Philautus (Anura: Rhacophoridae) from the Western Ghats, southwestern India. Current Herpetology, 22 (2), 51–60.

    https://doi.org/10.5358/hsj.22.51

    Li, J.T., Che, J., Bain, R.H., Zhao, E.M. & Zhang, Y.P. (2008) Molecular phylogeny of Rhacophoridae (Anura): A framework of taxonomic reassignment of species within the genera Aquixalus, Chiromantis, Rhacophorus, and Philautus. Molecular Phylogenetics and Evolution, 48 (1), 302–312.

    https://doi.org/10.1016/j.ympev.2008.03.023

    Li, J.T., Che, J., Murphy, R.W., Zhao, H., Zhao, E.M., Rao, D.Q. & Zhang, Y.P. (2009) New insights to the molecular phylogenetics and generic assessment in the Rhacophoridae (Amphibia: Anura) based on five nuclear and three mitochondrial genes, with comments on the evolution of reproduction. Molecular Phylogenetics and Evolution, 53 (2), 509–522.

    https://doi.org/10.1016/j.ympev.2009.06.023

    Mathew, R. & Sen, N. (2009) Studies on little known amphibians of Northeast India. Records of the Zoological Survey of India, Occasional Papers, 293, 1–64.

    Meegaskumbura, M., Meegaskumbura, S., Bowatte, G., Manamendra-Arachchi, K., Pethiyagoda, R., Hanken, J. & Schneider, C. (2011) Taruga (Anura: Rhacophoridae), a new genus of foam-nesting tree frogs endemic to Sri Lanka. Ceylon Journal of Science (Biological Sciences), 39 (2), 75–94.

    https://doi.org/10.4038/cjsbs.v39i2.2995

    Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. Gateway Computing Environments Workshop (GCE), 2010, 1–8.

    https://doi.org/10.1109/GCE.2010.5676129

    Myers, N., Mittermeier, R.A., Mittermeier, C.G., Da Fonseca, G.A. & Kent, J. (2000) Biodiversity hotspots for conservation priorities. Nature, 403 (6772), 853–858.

    https://doi.org/10.1038/35002501

    Nguyen, T.T., Matsui, M. & Duc, H.M. (2014) A new tree frog of the genus Kurixalus (Anura: Rhacophoridae) from Vietnam. Current Herpetology, 33 (2), 101–111.

    https://doi.org/10.5358/hsj.33.101

    Padhye, A.D., Sayyed, A., Jadhav, A. & Dahanukar, N. (2013) Raorchestes ghatei, a new species of shrub frog (Anura: Rhacophoridae) from the Western Ghats of Maharashtra, India. Journal of Threatened Taxa, 5 (15), 4913–4931.

    https://doi.org/10.11609/JoTT.o3702.4913-31

    Pillai, R.S. & Chanda, S.K. (1973) Philautus shillongensis, a new frog (Ranidae) from Meghalaya, India. Proceedings of the Indian Academy of Sciences B, 78 (1), 30–36.

    Posada, D. (2008) jModelTest: phylogenetic model averaging. Molecular Biology and Evolution, 25 (7), 1253–1256.

    https://doi.org/10.1093/molbev/msn083

    Rambaut, A. & Drummond, A.J. (2009) Tracer. Version 1.5. Available from: http://tree.bio.ed.ac.uk/software/tracer/ (accessed 25 January 2021)

    Roelants, K., Gower, D.J., Wilkinson, M., Loader, S.P., Biju, S.D., Guillaume, K., Moriau, L. & Bossuyt, F. (2007) Global patterns of diversification in the history of modern amphibians. Proceedings of the National Academy of Sciences of the USA, 104 (3), 887–892.

    https://doi.org/10.1073/pnas.0608378104

    Sambrook, J. Fritsch, E.F. & Maniatis, T. (1989) Molecular cloning: a laboratory manual. 2nd Edition. Cold Spring Harbor Laboratory Press, 1626 pp.

    Sarkar, A.K. & Ray, S. (2006) Amphibia. In: Alfred, J.R.B. (Ed.), Fauna of Arunachal Pradesh. State Fauna. Series 13. Part 1. Zoological Survey of India, Kolkata, pp. 303–307.

    Sarkar, A.K. & Sanyal, D.P. (1985) Amphibia. Records of the Zoological Survey of India, 81, 285–295.

    Seshadri, K.S., Gururaja, K.V. & Aravind, N.A. (2012) A new species of Raorchestes (Amphibia: Anura: Rhacophoridae) from mid-elevation evergreen forests of the southern Western Ghats, India. Zootaxa, 3410 (1), 19–34.

    https://doi.org/10.11646/zootaxa.3410.1.2

    Smith, M.A. (1924) 10. New Tree—Frogs from Indo-China and the Malay Peninsula. In: Proceedings of the Zoological Society of London. Vol. 94. No. 1. Blackwell Publishing Ltd., Oxford, pp. 225–234.

    https://doi.org/10.1111/j.1096-3642.1924.tb01499.x

    Stamatakis, A. (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics, 30 (9), 1312–1313.

    https://doi.org/10.1093/bioinformatics/btu033

    Sun, Y.B. (2018) FasParser2: a graphical platform for batch manipulation of tremendous amount of sequence data. Bioinformatics, 34 (14), 2493–2495.

    https://doi.org/10.1093/bioinformatics/bty126

    Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30 (12), 2725–2729.

    https://doi.org/10.1093/molbev/mst197

    Vijayakumar, S.P., Dinesh, K.P., Prabhu, M.V. & Shanker, K. (2014) Lineage delimitation and description of nine new species of bush frogs (Anura: Raorchestes, Rhacophoridae) from the Western Ghats Escarpment. Zootaxa, 3893 (4), 451–488.

    https://doi.org/10.11646/zootaxa.3893.4.1

    Vijayakumar, S.P., Menezes, R.C., Jayarajan, A. & Shanker, K. (2016) Glaciations, gradients, and geography: multiple drivers of diversification of bush frogs in the Western Ghats escarpment. Proceedings of the Royal Society B: Biological Sciences, 283 (1836), 20161011.

    https://doi.org/10.1098/rspb.2016.1011

    Wu, C., Yang, Z., Liu, C.X. & Zong, C. (2017) Molpa: A newly recorded genus (Orthoptera: Tettigoniidae: Phaneropterinae) from China. Zootaxa, 4365 (5), 585–589.

    https://doi.org/10.11646/zootaxa.4365.5.5

    Wu, S.P., Huang, C.C., Tsai, C.L., Lin, T.E., Jhang, J.J. & Wu, S.H. (2016) Systematic revision of the Taiwanese genus Kurixalus members with a description of two new endemic species (Anura, Rhacophoridae). ZooKeys, 557, 121–153.

    https://doi.org/10.3897/zookeys.557.6131

    Wu, Y.H., Suwannapoom, C., Xu, K., Chen, J.M., Jin, J.Q., Chen, H.M., Murphy, R.W. & Che, J. (2019) A new species of the genus Raorchestes (Anura: Rhacophoridae) from Yunnan Province, China. Zoological Research, 40 (6), 558–563.

    https://doi.org/10.24272/j.issn.2095-8137.2019.066

    Yang, D.T. & Li, S.M. (1978) s.n. In: Yang, D.T., Su, C.Y. & Li, S.M. (Eds.), Amphibians and Reptiles of Gaoligongshan, Kunming, 8, pp. 37–38.

    Yu, G., Liu, S., Hou, M., Li, S. & Yang, J. (2019) Extension in distribution of Raorchestes parvulus (Boulenger, 1893) (Anura: Rhacophoridae) to China. Zootaxa, 4577 (2), 381–391.

    https://doi.org/10.11646/zootaxa.4577.2.10

    Yu, G., Rao, D., Yang, J. & Zhang, M. (2008) Phylogenetic relationships among Rhacophorinae (Rhacophoridae, Anura, Amphibia), with an emphasis on the Chinese species. Zoological Journal of the Linnean Society, 153 (4), 733–749.

    https://doi.org/10.1111/j.1096-3642.2008.00404.x

    Yu, G., Rao, D., Zhang, M. & Yang, J. (2009) Re-examination of the phylogeny of Rhacophoridae (Anura) based on mitochondrial and nuclear DNA. Molecular Phylogenetics and Evolution, 50 (3), 571–579.

    https://doi.org/10.1016/j.ympev.2008.11.023

    Yu, G., Zhang, M. & Yang, J. (2010) A species boundary within the Chinese Kurixalus odontotarsus species group (Anura: Rhacophoridae): New insights from molecular evidence. Molecular Phylogenetics and Evolution, 56 (3), 942–950.

    https://doi.org/10.1016/j.ympev.2010.05.008

    Zachariah, A., Dinesh, K.P., Kunhikrishnan, E., Das, S., Raju, D.V., Radhakrishnan, C., Palot, M.J. & Kalesh, S. (2011) Nine new species of frogs of the genus Raorchestes (Amphibia: Anura: Rhacophoridae) from southern Western Ghats, India. Biosystematica, 5 (1), 25–48.

    Zhang, Y.Q., Li, L.Z. & Yin, Z.W. (2019) Fifteen new species and a new country record of Labomimus Sharp from China, with a checklist of world species (Coleoptera: Staphylinidae: Pselaphinae). Zootaxa, 4554 (2), 497–531.

    https://doi.org/10.11646/zootaxa.4554.2.7