Skip to main content Skip to main navigation menu Skip to site footer
Responsive image
Issue: Vol. 4894 No. 3: 10 Dec. 2020
Type: Article
Published: 2020-12-10
DOI: 10.11646/zootaxa.4894.3.5
Page range: 387–402
Abstract views: 214
PDF downloaded: 8

A new species of Limnonectes (Amphibia: Anura: Dicroglossidae) from Vietnam

Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, 10072 Hanoi, Vietnam.
Phu Yen University, 18 Tran Phu Road, Phu Yen Province, Vietnam.
Faculty of Environmental Sciences, University of Science, Vietnam National University, Hanoi, 334 Nguyen Trai Road, Hanoi, Vietnam. Central Institute for Natural Resources and Environmental Studies, Vietnam National University, Hanoi, 19 Le Thanh Tong, Hanoi, Vietnam. Department of Herpetology, American Museum of Natural History, Central Park West at 79th Street, New York 10024, USA.
Central Institute for Natural Resources and Environmental Studies, Vietnam National University, Hanoi, 19 Le Thanh Tong, Hanoi, Vietnam. Faculty of Biology, University of Science, Vietnam National University, Hanoi, 334 Nguyen Trai Road, Hanoi, Vietnam.
Asian Turtle Program - Indo-Myanmar Conservation, To Huu Street, Hanoi, Vietnam.
AG Zoologischer Garten Köln, Riehler Strasse 173, D-50735 Cologne, Germany. Institute of Zoology, University of Cologne, Zülpicher Strasse 47b, D-50674 Cologne, Germany.
Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, 10072 Hanoi, Vietnam. Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, 10072 Hanoi, Vietnam.
Amphibia Limnonectes phuyenensis sp. nov. molecular phylogeny taxonomy Phu Yen Province

Abstract

A new species of Limnonectes from Phu Yen Province, South-central Vietnam is described based on morphological and molecular differences. Morphologically, the new species is distinguishable from its congeners on the basis of a combination of the following diagnostic characters: SVL 49.8–77.9 mm in males, 40.8–61.8 mm in females; males with moderately enlarged head (HL/SVL 0.47, HW/SVL 0.48); head slightly broader than long (HL/HW 0.97 in males, 0.95 in females); vomerine teeth present; external vocal sacs absent; rostral length short (RL/SVL 0.15); tympanum invisible; dorsal surface of head, body and flanks with ridges and tubercles; dorsal surface of tibia and foot distinctly tuberculate, covered with moderately dense, small, low tubercles; supratympanic fold present; dorsolateral fold absent; Finger I with nuptial pad, composed of minute spines on dorsal surface and medial edge in males; toes webbed to distal of terminal phalanx, webbing formula I0–0II0–0III0–0IV0-–0V0; in life, dorsum yellowish brown with dark brown markings; ventral surface white with brown markings. In phylogenetic analyses, the new species from Vietnam and Limnonectes fastigatus as well as L. kiziriani form a clade, which is sister to L. fragilis from China, although the nodal support values for the set of relationships are insignificant from all analyses.

 

References

  1. Aowphol, A., Rujirawan, A., Taksintum, W., Chuaynkern, Y. & Stuart, B.L. (2015) A new caruncle-breeding Limnonectes (Anura: Dicroglossidae) from northeastern Thailand. Zootaxa, 3956 (2), 258–270.

    https://doi.org/10.11646/zootaxa.3956.2.6

    Borah, M.M., Bordoloi, S., Purkayastha, J., Das, M., Dubois, A. & Ohler, A. (2013) Limnonectes (Taylorana) medogensis (Fei, Ye & Huang, 1997) from Arunachal Pradesh (India), and on the identity of some diminutive ranoid frogs (Anura: Dicroglossidae, Occidozygidae). Herpetozoa, 26, 39–48.

    Bourret, R. (1942) Les Batraciens de l’Indochine. Institut Océanographique de l’Indochine, Hanoi, 517 pp.

    Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) JModelTest 2: More models, new heuristics and parallel computing. Nature Methods, 9, 772.

    https://doi.org/10.1038/nmeth.2109

    Emerson, S., Inger, R. & Iskandar, D. (2000) Molecular systematics and biogeography of the fanged frogs of Southeast Asia. Molecular Phylogenetics and Evolution, 16, 131–142.

    https://doi.org/10.1006/mpev.2000.0778

    Fei, L., Hu, S., Ye, C. & Huang, Y. (2009) Fauna Sinica. Amphibia. Volume 3. Anura. Science Press, Beijing, 887pp.

    Fei, L., Ye, C. & Jiang, J. (2012) Colored Atlas of Chinese Amphibians and Their Distributions. Sichuan Publishing House of Science & Technology, Sichuan, 619 pp.

    Fitzinger, L.J.F.J. (1843) Systema Reptilium. Fasciculus Primus. Braumüller et Seidel Bibliopolas, Wien, 120 pp.

    Frost, D.R. (2020) Amphibian Species of the World: an on-line reference. Version 6.0. American Museum of Natural History, New York. Available from https://amphibiansoftheworld.amnh.org/Amphibia/ (accessed on 10 April 2020)

    Glaw, F. & Vences, M. (2007) A field guide to the amphibians and reptiles of Madagascar. 3rd Edition. FroschVerlag, Cologne, 496 pp.

    Inger, R.F. (1947) Preliminary survey of the amphibians of the Riukiu islands. Fieldiana: Zoology, 32, 297–352.

    https://doi.org/10.5962/bhl.title.2991

    Inger, R.F. & Stuart, B.L. (2010) Systematics of Limnonectes (Taylorana) Dubois. Current Herpetology, 29, 51–68.

    https://doi.org/10.3105/018.029.0201

    Lambertz, M., Hartmann, T., Walsh, P., Geissler, P. & McLeod, D.S. (2014) Anatomy, histology, and systematic implications of the head ornamentation in the males of four species of Limnonectes (Anura: Dicroglossidae). Zoological Journal of the Linnean Society, 172, 117–132.

    https://doi.org/10.1111/zoj.12171

    Le, M., Raxworthy, C.J., McCord, W.P. & Mertz, L. (2006) A molecular phylogeny of tortoises (Testudines: Testudinidae) based on mitochondrial and nuclear genes. Molecular Phylogenetics and Evolution, 40, 517−531.

    https://doi.org/10.1016/j.ympev.2006.03.003

    Luu, V.Q., Nguyen, T.Q., Pham, C.T., Dang, K.N., Vu, T.N., Miskovic, S., Bonkowski, M. & Ziegler, T. (2014) No end in sight? Further new records of amphibians and reptiles from Phong Nha–Ke Bang National Park, Quang Binh Province, Vietnam. Biodiversity Journal, 4, 285–300.

    Matsui, M., Panha, S., Khonsue, W. & Kuraishi, N. (2010) Two new species of the “kuhlii” complex of the genus Limnonectes from Thailand (Anura: Dicroglossidae). Zootaxa, 2615 (1), 1–22.

    https://doi.org/10.11646/zootaxa.2615.1.1

    McLeod, D.S. (2008) A new species of big-headed, fanged dicroglossine frog (genus Limnonectes) from Thailand. Zootaxa, 1807 (1), 26–46.

    https://doi.org/10.11646/zootaxa.1807.1.2

    Mcleod, D.S. (2010) Of least concern? Systematics of a cryptic species complex: Limnonectes kuhlii (Amphibia: Anura: Dicroglossidae). Molecular Phylogenetics and Evolution, 56, 991–1000.

    https://doi.org/10.1016/j.ympev.2010.04.004

    McLeod, D.S., Kelly, J.K. & Barley, A. (2012) Same-same but different: Another new species of the Limnonectes kuhlii complex from Thailand (Anura: Dicroglossidae). Russian Journal of Herpetology, 19, 261–274.

    McLeod, D.S., Kurlbaum, S. & Hoang, N.V. (2015) More of the same: a diminutive new species of the Limnonectes kuhlii complex from northern Vietnam (Anura: Dicroglossidae). Zootaxa, 3947 (2), 201–214.

    https://doi.org/10.11646/zootaxa.3947.2.4

    Nguyen, L.T., Schmidt, H.A., von Haeseler, A. & Bui, M.Q. (2015) IQ-TREE: A fast and effective stochastic algorithm for estimating maximum likelihood phylogenies. Molecular Biology and Evolution, 32, 268–274.

    https://doi.org/10.1093/molbev/msu300

    Ohler, A. & Dubois, A. (1999) The identity of Elachyglossa gyldenstolpei Andersson, 1916 (Amphibia, Ranidae), with comments on some aspects of statistical support to taxonomy. Zoologica Scripta, 23, 269–279.

    https://doi.org/10.1046/j.1463-6409.1999.00002.x

    Palumbi, S.R., Martin, A., Romano, S., McMillan, W.O., Stice, L. & Grabowski, G. (1991) The simple fool’s guide to PCR. Department of Zoology and Kewalo Marine Laboratory, Hawaii, 94 pp.

    Pham, C.T., Le, M.D, Ngo, H.T., Ziegler, T. & Nguyen, T.Q. (2018) A new species of Limnonectes (Amphibia: Anura: Dicroglossidae) from Vietnam. Zootaxa, 4508, 115–130.

    https://doi.org/10.11646/zootaxa.4508.1.7

    Pham, C.T., Le, M.D, Nguyen, T.T., Ziegler, T., Wu, Z.J. & Nguyen, T.Q. (2017) A new species of Limnonectes (Amphibia: Anura: Dicroglossidae) from Vietnam. Zootaxa, 4269 (4), 545–558.

    https://doi.org/10.11646/zootaxa.4269.4.8

    Phimmachak, S., Richards, S.J., Sivongxay, N., Seateun, S., Chuaynkern, Y., Makchai, S., Som, H.E. & Stuart, B.L. (2019) A new caruncle-bearing fanged frog (Limnonectes, Dicroglossidae) from Laos and Thailand. ZooKeys, 846, 133−156.

    https://doi.org/10.3897/zookeys.846.33200

    Phimmachak, S., Sivongxay, N., Seateun, S., Yodthong, S., Rujirawan, A., Neang, T., Aowphol, A. & Stuart, B.L. (2018) A new Limnonectes (Anura: Dicroglossidae) from southern Laos. Zootaxa, 4375 (3), 325–340.

    https://doi.org/10.11646/zootaxa.4375.3.2

    Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61, 539−542.

    https://doi.org/10.1093/sysbio/sys029

    Simmons, J.E. (2002) Herpetological collecting and collections management. Revised edition. Society for the Study of Amphibians and Reptiles. Herpetological Circular, 31, 1–153.

    Stuart, B.L., Schoen, S.N., Nelson, E.E.M., Maher, H., Neang, T., Rowley, J.J.L. & McLeod D.S. (2020) A new fanged frog in the Limnonectes kuhlii complex (Anura: Dicroglossidae) from northeastern Cambodia. Zootaxa. [in press]

    Stuart, B.L., Sok, K. & Neang, T. (2006) A collection of amphibians and reptiles from hilly eastern Cambodia. Raffles Bulletin of Zoology, 54, 129–155.

    Suwannapoom, C., Yuan, Z., Chen, J.M., Hou, M., Zhao, H., Wang, L., Nguyen, T.Q., Murphy, R.W., Sullivan, S., McLeod, D.S. & Che, J. (2016) Taxonomic revision of the Chinese Limnonectes (Anura, Dicroglossidae) with the description of a new species from China and Myanmar. Zootaxa, 4093 (4), 181–200. [Erratum, 4137 (4), 599–600.]

    https://doi.org/10.11646/zootaxa.4137.4.13

    Swofford, D.L. (2001) PAUP*. Phylogenetic Analysis Using Parsimony (* and Other Methods), version 4. Sinauer Associates, Sunderland, Massachusetts. [software]

    Taylor, E.H. (1962) The amphibian fauna of Thailand. University of Kansas Science Bulletin, 43, 265–599.

    https://doi.org/10.5962/bhl.part.13347

    Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. & Higgins, D.G. (1997) The ClustalX windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25, 4876−4882.

    https://doi.org/10.1093/nar/25.24.4876

    Yang, D. (1983) A new species of the genus Platymantis (Amphibia: Ranidae). Acta Herpetologica Sinica, 2, 53–56.

    Ye, C.Y., Fei, L., Xie, F. & Jiang, J.P. (2007) A new Ranidae species from China–Limnonectes bannanensis (Ranidae: Anura). Zoological Research, 28, 545–550.