Abstract
We describe a new species of mud turtle of the genus Kinosternon from the Pacific Coastal Plain of the Mexican states Sinaloa and Nayarit. The new species shares morphological characters with the recently described Kinosternon vogti, which are unique to these two turtles and separate them from the other species of the genus. The new species differs from K. vogti by skin coloration, size, and the scutellation of both carapace and plastron. We also present a molecular phylogeny of the family Kinosternidae based on two mitochondrial and four nuclear loci. Our results show that the new species is most closely related to K. vogti, and together they form the sister group to the K. hirtipes and K. integrum species groups of Kinosternon.
References
Agassiz, L. (1857) Contributions to the Natural History of the United States of America. Vol. 1. Little, Brown & Co., Boston, lii + 452 pp.
https://doi.org/10.5962/bhl.title.12644
Berry, F.J. & Berry, C.M. (1984) A re-analysis of geographic variation and systematics in the Yellow Mud Turtle, Kinosternon flavescens (Agassiz). Annals of Carnegie Museum of Natural History, 53 (7), 185–206.
[https://www.biodiversitylibrary.org/item/216357#page/203/mode/1up]
Berry, J.F. & Iverson, J.B. (1980) A new species of mud turtle, genus Kinosternon, from Oaxaca, Mexico. Journal of Herpetology, 14 (4), 313–320.
https://doi.org/10.2307/1563684
Berry, J.F. & Legler, J.M. (1980) A new turtle (genus Kinosternon) from northwestern Mexico. Contributions in Science, Natural History Museum of Los Angeles County, 325, 1–12.
[https://www.biodiversitylibrary.org/item/214422#page/29/mode/1up]
Berry, J.F., Seidel, M.F. & Iverson, J.B. (1997) A new species of mud turtle (genus Kinosternon) from Jalisco and Colima, Mexico, with notes on its natural history. Chelonian Conservation and Biology, 2 (3), 329–337.
Bonnaterre, P.J. (1789) Tableau encyclopédique et méthodique des trois règnes de la nature. Erpétologie. Panckoucke, Paris, xxviii + 71 pp.
https://doi.org/10.5962/bhl.title.11660
Chávez-Avila, S.M., Casas-Andreu, G., García-Aguayo, A., Cifuentes-Lemus, J.L. & Cupul-Magaña, F.G. (2015) Anfibios y reptiles del estado de Jalisco. Análisis espacial, distribución y conservación. Universidad de Guadalajara, Puerto Vallarta, 101 pp. ISBN: 978-607-742-318-8. [http://www.cuc.udg.mx/sites/default/files/publicaciones/2015%20-%20Anfibios%20y%20reptiles%20del%20estado%20de%20Jalisco.pdf]
Cupul-Magaña, F.G. & Rubio-Delgado, A. (2003) Distribución de dos especies de tortugas dulceacuícolas, Kinosternon chimalhuaca y Chelydra serpentina (Testudines), en Puerto Vallarta, Jalisco; México. Boletín de la Sociedad Herpetológica Mexicana, 11 (1), 49–50.
Duméril, A.M.C. & Duméril, A.H.A. (1851) Catalogue méthodique de la collection des reptiles du Muséum d’Histoire Naturelle de Paris. Gide et Baudry/Roret, Paris, 224 pp.
Edwards, T., Karl, A., Augh, M., Rosen, P., Meléndez-Torres, C. & Murphy, R.W. (2016) The desert tortoise trichotomy: Mexico hosts a third new sister-species of tortoise in the Gopherus morafkai-G. agassizii group. Zookeys, 562, 131–158.
https://doi.org/10.3897/zookeys.562.6124
Flores-Villela, O., Smith, H.M. & Chiszar, D. (2004) The history of herpetological exploration in Mexico. Bonner Zoologische Beiträge, 3 (4), 311–335.
García, A. (2006) Using ecological niche modelling to identify diversity hotspots for the herpetofauna of Pacific lowlands and adjacent interior valleys of Mexico. Biological Conservation, 130 (1), 25–46.
https://doi.org/10.1016/j.biocon.2005.11.030
Garman, S. (1891) On a tortoise found in Florida and Cuba, Cinosternon baurii. Bulletin of Essex Institute, Salem, Massachusetts, 23, 141–144
Gilmore, C.W. (1923) A new fossil turtle, Kinosternon arizonense, from Arizona. Proceedings of the United States National Museum, 62 (2451), 1–8.
https://doi.org/10.5479/si.00963801.62-2451.1
Goldman, E.A. (1951) Biological investigations in Mexico. Smithsonian Miscellaneous Collections, 115, 1–476.
https://www.biodiversitylibrary.org/item/80431#page/7/mode/1up
Gray, J.E. (1831a) Synopsis Reptilium or short descriptions of the species of reptiles. Part I. Cataphracta, tortoises, crocodiles, and enaliosaurians. Treuttel, Wurz & Co., London, 85 pp.
https://doi.org/10.5962/bhl.title.4697
Gray, J.E. (1831b) A synopsis of the species of Class Reptilia. In: Griffith, E & E. Pidgeon: The animal kingdom arranged in conformity with its organisation by the Baron Cuvier with additional descriptions of all the species hither named, and of many before noticed. Vol. 9. Whittaker, Treacher and Co., London, 481 + 110 pp.
Hardy, L.M. & McDiarmid, R.W. (1969) The amphibians and reptiles of Sinaloa, Mexico. University of Kansas Publications, Museum of Natural History, 18 (3), 39–252.
https://doi.org/10.5962/bhl.part.19990
Hartweg, N. (1934) Description of a new kinosternid from Yucatan. Occasional Papers of the Museum of Zoology, University of Michigan, 277, 1–2.
https://deepblue.lib.umich.edu/handle/2027.42/56716
Hartweg, N. (1938) Kinosternon flavescens stejnegeri, a new turtle from northern Mexico. Occasional Papers of the Museum of Zoology, University of Michigan, 371, 1–5.
Hulse, A.C. (1976) Growth and morphometrics of Kinosternon sonoriense (Reptilia, Testudines, Kinosternidae). Journal of Herpetology, 10 (4), 341–348.
https://doi.org/10.2307/1563072
Ippi, S. & Flores, V. (2001) Las tortugas neotropicales y sus áreas de endemismo. Acta Zoológica Mexicana, 84, 49–63.
Iverson, J.B. (1979a) Another inexpensive turtle trap. Herpetological Review, 10 (2), 55.
Iverson, J.B. (1979b) A taxonomic reappraisal of the yellow mud turtle, Kinosternon flavescens (Testudines: Kinosternidae). Copeia, 1979 (2), 212–225.
https://doi.org/10.2307/1443406
Iverson, J.B. (1981) Biosystematics of the Kinosternon hirtipes group (Testudines, Kinosternidae). Tulane Studies of Zoology and Botany, 23 (1), 1–74.
[https://www.biodiversitylibrary.org/item/30378#page/17/mode/1up]
Iverson, J.B. (1989) The Arizona mud turtle, Kinosternon flavescens arizonense (Kinosternidae), in Arizona and Sonora. The Southwestern Naturalist, 34 (3), 356–368.
https://doi.org/10.2307/3672164
Iverson, J.B., Le, M. & Ingram, C. (2013) Molecular phylogenetics of the mud and musk turtle family Kinosternidae. Molecular Phylogenetics and Evolution, 69 (3), 929–939.
https://doi.org/10.1016/j.ympev.2013.06.011
Katoh, K. & Standley, D.M. (2013) MAFFT Multiple Sequence Alignment Software Version 7: Improvements in performance and usability, Molecular Biology and Evolution, 30 (4), 772–780.
https://doi.org/10.1093/molbev/mst010
Lanfear, R., Calcott, B., Ho, S.Y.W. & Guindon, S. (2012) PartitionFinder: Combined selection of partitioning schemes and substitution models for phylogenetic analyses. Molecular Biology and Evolution, 29 (6), 1695–1701.
https://doi.org/10.1093/molbev/mss020
LeConte, J. (1854) Description of four new species of Kinosternum. Proceedings of the Academy of Natural Sciences of Philadelphia, 7, 180–190.
[https://www.biodiversitylibrary.org/item/17735#page/202/mode/1up]
Legler, J. (1965) A new species of turtle, genus Kinosternon, from Central America. University of Kansas Publications, Museum of Natural History, 15 (13), 615–625.
https://doi.org/10.5962/bhl.part.7292
Legler, J. & Vogt, R.C. (2013) Turtles of Mexico: Land and Freshwater Forms. University of California Press, Berkeley, 402 pp.
https://doi.org/10.1525/9780520956896
Linnaeus, C. (1766) Systema naturæ per regna tria naturæ, secundum classes, ordines, genera, species, cum characteribus, differentiis, synonymis, locis. Tomus I. Editio duodecima, reformata. Laurentii Salvii, Stockholm, Holmiae, 532 pp.
https://doi.org/10.5962/bhl.title.68927
López-Luna, M.A., Cupul-Magaña, F.G., Escobedo-Galván, A.H., González-Hernández, A.J., Centenero-Alcalá, E., Rangel-Mendoza, J.A., Ramírez-Ramírez, M.M. & Cazares-Hernández, E. (2018) A distinctive new species of Mud Turtle from Western México. Chelonian Conservation and Biology, 17 (1), 2–13.
https://doi.org/10.2744/CCB-1292.1
Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. 2010 Gateway Computing. Environments Workshop GCE, 2010, 1–8.
https://doi.org/10.1109/GCE.2010.5676129
Murphy, R.W., Berry, K.H., Edwards, T., Leviton, A.E., Lathrop, A. & Riedle, J.D. (2011) The dazed and confused identity of Agassiz’s land tortoise, Gopherus agassizii (Testudines, Testudinidae) with the description of a new species, and its consequences for conservation. ZooKeys, 113, 39–71.
https://doi.org/10.3897/zookeys.113.1353
Rambaut, A., Suchard, M.A., Xie, D. & Drummond, A.J. (2014) Tracer v1.6, Available from http://beast.bio.ed.ac.uk/Tracer (accessed 28 August 2020)
Rambaut, A. & Drummond, A.J. (2014) TreeAnnotator. Version 1.8.2. Available from https://beast.community/treeannotator (accessed 28 August 2020)
Ronquist, F., Teslenko, M., Mark, P. van der, Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61 (3), 539–542.
https://doi.org/10.1093/sysbio/sys029
Schmidt, K.P. (1947) A new kinosternid turtle from Colombia. Fieldiana: Zoology, 31 (13), 109–112.
https://doi.org/10.5962/bhl.title.3075
Spinks, P.Q., Thomson, R., Müge, G. & Shaffer, H.B. (2014) Multilocus phylogeny of the New-World Mud Turtles (Kinosternidae) supports the traditional classification of the group. Molecular Phylogenetics and Evolution, 76, 254–260.
https://doi.org/10.1016/j.ympev.2014.03.025
Stejneger, L.H. (1925) New species and subspecies of American turtles. Journal of Washington Academy of Sciences, 15 (20), 462–463.
[https://www.biodiversitylibrary.org/item/123269#page/552/mode/1up]
Turtle Taxonomy Working Group [Rhodin, A.G.J., Iverson, J.B., Bour, R. Fritz, U., Georges, A., Shaffer, H.B. & van Dijk, P.P.] (2017) Turtles of the World: Annotated Checklist and Atlas of Taxonomy, Synonymy, Distribution, and Conservation Status. 8th Edition. In: Rhodin, A.G.J., Iverson, J.B., van Dijk, P.P., Saumure, R.A., Buhlmann, K.A., Pritchard, P.C.H. & Mittermeier, R.A. (Eds.), Conservation Biology of Freshwater Turtles and Tortoises: A Compilation Project of the IUCN/SSC Tortoise and Freshwater Turtle Specialist Group. Chelonian Research Monographs, 7, pp. 1–292.
https://doi.org/10.3854/crm.7.checklist.atlas.v8.2017
Underwood, W. & Anthony, R. (2020) AVMA Guidelines for the Euthanasia of Animals: 2020 Edition. Retrieved 30 March 2020, 2020-01. Available from https://www.avma.org/sites/default/files/2020-01/2020-Euthanasia-Final-1-17-20.pdf (accessed 28 August 2020)
Vaidya, G., Lohman, D.J. & Meier, R. (2011) SequenceMatrix: concatenation software for the fast assembly of multi-gene datasets with character set and codon information. Cladistics, 27 (2), 171–180.
https://doi.org/10.1111/j.1096-0031.2010.00329.x
Van Denburgh, J. (1897) Reptiles from Sonora, Sinaloa and Jalisco, Mexico, with a description of a new species of Sceloporus. Proceedings of the Academy of Natural Sciences of Philadelphia, 49, 460–64.
https://www.jstor.org/stable/4062303
Wagler, J.G. (1830) Natürliches System der Amphibien, mit vorangehender Classification der Säugetiere und Vögel. Ein Beitrag zur vergleichenden Zoologie. Cotta, München, Stuttgart, and Tübingen, 354 pp.
https://doi.org/10.5962/bhl.title.58730
Webb, R.G. (2001) The Jalisco Mud Turtle, Kinosternon chimalhuaca, in northern Nayarit, Mexico. Herpetological Review, 32 (2), 87–88.
Wiegmann, A.F.A. (1834) Herpetologia Mexicana, seu descriptio amphibiorum novae hispaniae, quae itineribus comitis de Sack, Ferdinandi Deppe et Chr. Guil. Schiede im Museum Zoologicum Berolinense Pervenerunt. Pars prima, saurorum species. Berlin, Lüderitz, iv + 54 pp.
https://doi.org/10.5962/bhl.title.119131
Woolrich-Piña, G.A., Ponce-Campos, P., Loc-Barragán, J.A., Ramírez-Silva, J.P., Mata-Silva, V., Johnson, J.D. & Wilson, L.D. (2016) The herpetofauna of Nayarit, Mexico: composition, distribution, and conservation. Mesoamerican Herpetology, 3 (2), 376–448.
Zarza, E., Reynoso, V.H. & Emerson, B.C. (2011) Discordant patterns of geographic variation between mitochondrial and microsatellite markers in the Mexican black iguana (Ctenosaura pectinata) in a contact zone. Journal of Biogeography, 38 (7), 1394–1405.
https://doi.org/10.1111/j.1365-2699.2011.02485.x
Zarza, E., Reynoso, V.H., Faria, C.M. & Emerson, B.C. (2019) Introgressive hybridization in a Spiny-Tailed Iguana, Ctenosaura pectinata, and its implications for taxonomy and conservation. PeerJ, 7, e6744.
https://doi.org/10.7717/peerj.6744