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Type: Article
Published: 2020-05-01
Page range: 282–290
Abstract views: 79
PDF downloaded: 3

DNA barcodes and morphology confirm a new species of Rheocricotopus (Psilocricotopus) orientalis group (Diptera: Chironomidae)

College of Life Sciences, Nankai University, Tianjin, 300071, P.R. China
Key Laboratory of Aquatic Ecology and Aquaculture of Tianjin, College of Fishery, Tianjin Agricultural University, Tianjin, 300384, P.R. China
Key Laboratory of Aquatic Ecology and Aquaculture of Tianjin, College of Fishery, Tianjin Agricultural University, Tianjin, 300384, P.R. China
College of Life Sciences, Nankai University, Tianjin, 300071, P.R. China
College of Life Sciences, Nankai University, Tianjin, 300071, P.R. China
Diptera Taxonomy COI Orthocladiinae key China

Abstract

Morphology and DNA barcodes confirm a new chironomid species within the Rheocricotopus (Psilocricotopus) orientalis group (Diptera: Chironomidae). Rheocricotopus (Psilocricotopus) kongi Lin et Wang sp. n. is described and illustrated based on adult male from Hainan, China. Key to adult males of the R. orientalis group is given.

 

References

  1. Anderson, A.M., Stur, E. & Ekrem, T. (2013) Molecular and morphological methods reveal cryptic diversity and three new species of Nearctic Micropsectra (Diptera: Chironomidae). Freshwater Science, 32, 892–921.

    https://doi.org/10.1899/12-026.1

    Ashe, P. & O’Connor, J. (2012) A World Catalogue of Chironomidae (Diptera). Part 2. Orthocladiinae. Irish Biogeographical Society and National Museum of Ireland. Dublin. xvi,

    Edgar, R.C. (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research, 32, 1792–1797.

    https://doi.org/10.1093/nar/gkh340

    Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3, 294–299.

    Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35, 1547–1549.

    https://doi.org/10.1093/molbev/msy096

    Lin, X.L., Stur, E. & Ekrem, T. (2015) Exploring genetic divergence in a species-rich insect genus using 2790 DNA Barcodes. PloS One, 10, e0138993.

    https://doi.org/10.1371/journal.pone.0138993

    Lin, X.L., Stur, E. & Ekrem, T. (2018) DNA barcodes and morphology reveal unrecognized species of Chironomidae (Diptera). Insect Systematics & Evolution, 49, 329–398.

    https://doi.org/10.1163/1876312X-00002172

    Lin, X.L., Yu, H.J., Zhang, R.L. & Wang, X.H. (2019) Polypedilum (Cerobregma) heberti sp. n. (Diptera: Chironomidae) from Gaoligong Mountains, Yunnan, China. Zootaxa, 4571 (2), 255–262.

    https://doi.org/10.11646/zootaxa.4571.2.5

    Moubayed-Breil, J. & Ashe, P. (2019) Rheocricotopus (s. str.) costai sp. n. AND R. (s. str.) pyrenaeus sp. n., TWO RELICT SPECIES FROM GLACIAL RHEOCRENES AND STREAMS IN CORSICA AND THE EASTERN PYRENEES (DIPTERA: CHIRONOMIDAE, ORTHOCLADIINAE). Trakya University Journal of Natural Sciences, 20, 35–46.

    https://doi.org/10.23902/trkjnat.493928

    Ratnasingham, S. & Hebert, P.D.N. (2007) BOLD: The Barcode of Life Data System (www.barcodinglife.org). Molecular Ecology Notes, 7, 355–364.

    https://doi.org/10.1111/j.1471-8286.2006.01678.x

    Sæther, O.A. (1969) Some Nearctic Podonominae, Diamesinae, and Orthocladiinae (Diptera: Chironomidae). Bulletin of the Fisheries Research Board of Canada, 170, 1–154.

    Sæther, O.A. (1980) Glossary of chironomid morphology terminology (Diptera: Chironomidae). Entomologica Scandinavica, Supplement 14, 1–51.

    Song, C., Lin, X.L., Wang, Q. & Wang, X.H. (2018) DNA barcodes successfully delimit morphospecies in a superdiverse insect genus. Zoologica Scripta, 47, 311–324.

    https://doi.org/10.1111/zsc.12284

    Wang, X.H. (1995) Rheocricotopus (R.) orientalis a new species from China (Diptera: Chironomidae). Aquatic Insects, 17, 37–40.

    https://doi.org/10.1080/01650429509361567

    Wang, X.H. & Sæther, O.A. (2001) Two new species of the orientalis group of Rheocricotopus (Psilocricotopus) from China (Diptera: Chironomidae). Hydrobiologia, 444, 237–240.

    https://doi.org/10.1023/A:1017548204492

    Yan, C.C. & Wang, X.H. (2004) A new species of the orientalis group of Rheocricotopus from China (Diptera: Chironomidae). Acta Zootaxonomica Sinica, 29, 560–562.