Skip to main content Skip to main navigation menu Skip to site footer
Type: Article
Published: 2019-12-11
Page range: 531–545
Abstract views: 91
PDF downloaded: 2

A new species of Ischnocnema Reinhardt and Lütken, 1862 (Anura: Brachycephalidae) of the I. lactea species series from southeastern Brazil

Instituto de Biociências, UNESP – Universidade Estadual Paulista, Câmpus Rio Claro, Departamento de Zoologia e Centro de Aquicultura (CAUNESP), Cx. Postal 199, 13506-900, Rio Claro, SP, Brazil
Instituto de Ciências Biológicas, UFMG – Universidade Federal de Minas Gerais, Departamento de Zoologia, Laboratório de Herpetologia, Avenida Antônio Carlos, 6627, Pampulha, 31270-910, Belo Horizonte, MG, Brazil
Museu de Zoologia da Universidade de São Paulo, Avenida Nazaré 481, São Paulo, SP, CEP 04263-000, Brazil
Instituto de Ciências Biológicas, UFMG – Universidade Federal de Minas Gerais, Departamento de Zoologia, Laboratório de Herpetologia, Avenida Antônio Carlos, 6627, Pampulha, 31270-910, Belo Horizonte, MG, Brazil
Atlantic Rainforest Bioacoustics Brachycephaloidea Integrative taxonomy Molecular phylogeny

Abstract

We describe a new species of Ischnocnema from the Serra da Bocaina mountain range, state of São Paulo, southeastern Brazil, based on morphological, bioacoustic, and mtDNA data. The new species is retrieved with high support values within the I. lactea species series as the sister species of I. spanios. Ischnocnema bocaina sp. nov. is characterized by its medium size (18.6–19.0 mm), a smooth venter, a rounded snout in dorsal view and acuminate in lateral view, a slightly expanded subgular, single vocal sac, a round and whitish, poorly-developed glandular-appearing nuptial pad on the dorsal surface of the thumb, and a nonpulsed advertisement call with 9 to 18 notes. We raise to 38 the number of Ischnocnema species, the 12th described in the past 10 years.

References

  1. Berneck, B.V.M., Targino, M. & Garcia, P.C.A. (2013) Rediscovery and re-description of Ischnocnema nigriventris (Lutz, 1925) (Anura: Terrarana: Brachycephalidae). Zootaxa, 3694 (2), 131–142.

    https://doi.org/10.11646/zootaxa.3694.2.2

    Bioacoustics Research Program (2011) Raven Pro: Interactive Sound Analysis. The Cornell Lab of Ornithology, Ithaca, New York. [program]

    Bokermann, W.C.A. (1975 “1974”) Três espécies novas de Eleutherodactylus do sudeste da Bahia, Brasil (Anura, Leptodactylidae). Revista Brasileira De Biologia, 34, 11–18.

    Brown, S.D.J., Collins, R.A., Boyer, S., Lefort, M.C., Malumbres-Olarte, J., Vink, C.J. & Cruickshank, R.H. (2012) Spider: An R package for the analysis of species identity and evolution, with particular reference to DNA barcoding. Molecular Ecology Resources, 12, 562–565.

    https://doi.org/10.1111/j.1755-0998.2011.03108.x

    Canedo, C. & Haddad, C.F.B. (2012) Phylogenetic relationships within anuran clade Terrarana, with emphasis on the placement of Brazilian Atlantic rainforest frogs genus Ischnocnema (Anura: Brachycephalidae). Molecular Phylogenetics and Evolution, 65, 610–620.

    https://doi.org/10.1016/j.ympev.2012.07.016

    Castanho, L.M. & Haddad, C.F.B. (2000) New Species of Eleutherodactylus (Amphibia : Leptodactylidae) from Guaraqueçaba. Atlantic Forest of Brazil. Copeia 2000, 777–781.

    https://doi.org/10.1643/0045-8511(2000)000[0777:NSOEAL]2.0.CO;2

    Cochran, D.M. (1948) A new subspecies of frog from Itatiaya, Brazil. American Museum Novitates, 1375, 1–3.

    Duellman, W.E. (1970) The Hylid of Middle America. University of Kansas, Lawrence, 444 pp.

    https://doi.org/10.2307/1442281

    Duellman, W.E. & Lehr, E. (2009) Terrestrial-Breeding Frogs (Strabomantidae) in Peru. Natur und Tier - Verlag GmbH, Münster, 382 pp.

    Fouquet, A., Gilles, A., Vences, M., Marty, C., Blanc, M. & Gemmell, N.J. (2007) Underestimation of Species Richness in Neotropical Frogs Revealed by mtDNA Analyses. PLoS ONE, 2, 1–10.

    https://doi.org/10.1371/journal.pone.0001109

    Frost, D.R. (2018) Amphibian Species of the World: an Online Reference. American Museum of Natural History, New York. Available from: http://research.amnh.org/herpetology/amphibia/index.html (accessed 18 December 2018)

    Garcia, P.C.A. (1996) Nova espécie de Eleutherodactylus Duméril & Bibron, 1891 (Amphibia, Anura, Leptodactylidae) do Estado de Santa Catarina, Brasil. Biociências, 4, 57–68.

    Garcia, P.C.A., Vinciprova, G. & Haddad, C.F.B. (2003) the Taxonomic Status of Hyla Pulchella Joaquini (Anura: Hylidae) With Description of Its Tadpole and Vocalization. Herpetologica, 59, 350–363.

    https://doi.org/10.1655/01-54

    Gehara, M., Barth, A., Oliveira, E.F.de., Costa, M.A., Haddad, C.F.B. & Vences, M. (2017) Model-based analyses reveal insular population diversification and cryptic frog species in the Ischnocnema parva complex in the Atlantic forest of Brazil. Molecular Phylogenetics and Evolution, 112, 68–78.

    https://doi.org/10.1016/j.ympev.2017.04.007

    Gehara, M., Canedo, C., Haddad, C.F.B. & Vences, M. (2013) From widespread to microendemic: Molecular and acoustic analyses show that Ischnocnema guentheri (Amphibia: Brachycephalidae) is endemic to Rio de Janeiro, Brazil. Conservation Genetics, 14, 973–982.

    https://doi.org/10.1007/s10592-013-0488-5

    Guindon, S., Dufayard, J.F., Lefort, V., Anisimova, M., Hordijk, W. & Gascuel, O. (2010) New algorithms and methods to estimate maximum-likelihood phylogenies: Assessing the performance of PhyML 3.0. Systematic Biology, 59, 307–321.

    https://doi.org/10.1093/sysbio/syq010

    Günther, A. (1858) On the Systematic Arrangement of the Tailless Batrachians And The Structure of Rhinophrynus dorsalis. Proceedings of the Zoological Society of London, 26, 339–352.

    https://doi.org/10.1111/j.1469-7998.1858.tb06387.x

    Hedges, S.B., Duellman, W.E. & Heinicke, M.P. (2008) New World direct-developing frogs (Anura: Terrarana): Molecular phylogeny, classification, biogeography, and conservation. Zootaxa, 1737, 1–182.

    https://doi.org/10.11646/zootaxa.1737.1.1

    Hepp, F. & Canedo, C. (2013) Advertisement and aggressive calls of Ischnocnema oea (Heyer, 1984) (Anura, Brachycephalidae). Zootaxa, 3710 (2), 197–199.

    https://doi.org/10.11646/zootaxa.3710.2.6

    Heyer, W.R. (1984) Variation, systematics, and zoogeography of Eleutherodactylus guentheri and closely related species (Amphibia: Anura: Leptodactylidae). Smith Contributions to Zoolology, 402, 15342.

    https://doi.org/10.5479/si.00810282.402

    Heyer, W.R. (1985) New species of frogs from Boracéia, São Paulo, Brazil. Proceedings of the Biological Society of Washington, 98, 657–671.

    Heyer, W.R., Rand, A.S., Cruz, C.A.G. Da, Peixoto, O.L. & Nelson, C.E. (1990) Frogs of Boraceia. Arquivos de Zoologia, 31, 235–410.

    Hurvich, C. & Tsai, C. (1989) Regression and time series model selection in small samples. Biometrika, 76, 297–307.

    https://doi.org/10.1093/biomet/76.2.297

    Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: Improvements in performance and usability. Molecular Biology and Evolution, 30, 772–780.

    https://doi.org/10.1093/molbev/mst010

    Köhler, J., Jansen, M., Rodríguez, A., Kok, P.J.R., Toledo, L.F., Emmrich, M., Glaw, F., Haddad, C.F.B., Rödel, M.O. & Vences, M. (2017) 4251 Zootaxa The use of bioacoustics in anuran taxonomy: Theory, terminology, methods and recommendations for best practice. Zootaxa, 4251 (1), 1–124.

    https://doi.org/10.11646/zootaxa.4251.1.1

    Lanfear, R., Frandsen, P.B., Wright, A.M., Senfeld, T. & Calcott, B. (2017) Partitionfinder 2: New methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses. Molecular Biology and Evolution, 34, 772–773.

    https://doi.org/10.1093/molbev/msw260

    Langone, J.A. & Segalla, M.V. (1996) Una nueva especie de Eleutherodactylus del Estado de Paraná, Brasil. Comunicaciones Zoologicas del Museo de Historia Natural de Montevideo, 12, 1–5.

    Ligges, U., Krey, S., Mersman, O. & Schnackenberg, S. (2013) tuneR: Analysis of music. Available from: http://r-forge.r-project.org/projects/tuner (accessed 21 October 2019)

    Lutz, A. (1925) Batraciens du Brésil. Comptes Rendus et Mémoires Hebdomadaires des Séances de la Société de Biologie et des ses Filiales, 22, 211–214.

    Lutz, B. (1958) Anfíbios novos e raros das Serras Costeiras do Brasil: Eleutherodactylus venancioi n.sp., E. hoehnei n.sp., Holoaden bradei n.sp. e H. lüderwaldti Mir. Rib., 1920. Memórias do Instituto Oswaldo Cruz, 56, 372–389.

    https://doi.org/10.1590/S0074-02761958000200002

    Lynch, J.D. & Duellman, W.E. (1997) Frogs of the Genus Eleutherodactylus in Western Ecuador Biogeography. Special Publication of the Natural History Museum, The University of Kansas, 23, 1–236.

    Lyra, M.L., Haddad, C.F.B. & de Azeredo-Espin, A.M.L. (2017) Meeting the challenge of DNA barcoding Neotropical amphibians: polymerase chain reaction optimization and new COI primers. Molecular Ecology Resources, 17, 966–980.

    https://doi.org/10.1111/1755-0998.12648

    Martins, I.A. & Haddad, C.F.B. (2010) A new species of Ischnocnema from highlands of the Atlantic forest, Southeastern Brazil (Terrarana, Brachycephalidae). Zootaxa, 2617 (1), 55–65.

    https://doi.org/10.11646/zootaxa.2617.1.2

    Miranda-Ribeiro, A. (1923) Basanitia lactea (Um novo batracchio das collecções do Museu Paulista). Revista do Museu Paulista, 13, 851–852.

    Miranda-Ribeiro, A. (1926) Notas para servirem ao estudo dos Gymnobatrachios (Anura) brasileiros. Arquivos do Museu Nacional, 27, 1–227.

    Padial, J.M., Grant, T. & Frost, D.R. (2014) Molecular systematics of terraranas (Anura: Brachycephaloidea) with an assessment of the effects of alignment and optimality criteria. Zootaxa, 3825 (1), 1–132.

    https://doi.org/10.11646/zootaxa.3825.1.1

    Palumbi, S.R., Martin, A.P., Kessing, B.D. & McMillan, W.O. (1991) Detecting population structure using mitochondrial DNA. In: Hoelzel, A.R. (Ed.), Genetic Ecology of Whales and Dolphins. International Whaling Comission, Cambridge, pp. 203–215.

    Paradis, E., Claude, J. & Strimmer, K. (2004) APE: Analyses of phylogenetics and evolution in R language. Bioinformatics, 20, 289–290.

    https://doi.org/10.1093/bioinformatics/btg412

    Pombal, Jr.J.P. & da Cruz, C.A.G. (1999) Redescrição de Eleutherodactylus bolbodactylus (A. Lutz, 1925) e a posição taxonômica de E. gehrti (Miranda-Ribeiro, 1926) (Anura, Leptodactylidae). Boletim do Museu Nacional, Nova Série, Zoologia, 404, 1–10.

    R Core Team (2018) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna. [program]

    Reinhardt, J.T. & Lütken, C.F. (1862 “1861”) Bidrag til Kundskab om Brasiliens Padder og Krybdyr. Förste Afdeling: Padderne og Öglerne. Videnskabelige Meddelelser fra Dansk Naturhistorisk Forening i Kjøbenhavn, 2, 143–242.

    Ronquist, F., Teslenko, M., Van Der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) Mrbayes 3.2: Efficient bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61, 539–542.

    https://doi.org/10.1093/sysbio/sys029

    Silva-Soares, T., Malagoli, L.R., Cruz, C.A.G. & Caramaschi, U. (2018) On the taxonomy and natural history of the secretive Ischnocnema lactea Miranda-Ribeiro, 1923) (Anura: Brachycephaloidea: Brachycephalidae). Zootaxa, 4444 (2), 137–153.

    https://doi.org/10.11646/zootaxa.4444.2.3

    Stamatakis, A. (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics, 30, 1312–1313.

    https://doi.org/10.1093/bioinformatics/btu033

    Sueur, J., Aubin, T. & Simonis, C. (2008) Seewave , a free modular tool for sound analysis and synthesis. Bioacoustics, 18, 213–226.

    https://doi.org/10.1080/09524622.2008.9753600

    Targino, M. & de Carvalho-e-Silva, S.P. (2008) Redescrição de Ischnocnema holti (Amphibia: Anura: Brachycephalidae). Revista Brasileira de Zoologia, 25, 716–723.

    https://doi.org/10.1590/S0101-81752008000400017

    Targino, M., Costa, P.N. & de Carvalho-e-Silva, S.P. (2009) Two New Species of the Ischnocnema lactea Species Series from Itatiaia Highlands, Southeastern Brazil (Amphibia, Anura, Brachycephalidae). South American Journal of Herpetology, 4, 139–150.

    https://doi.org/10.2994/057.004.0205

    Taucce, P.P.G., Canedo, C. & Haddad, C.F.B. (2018a) Two new species of Ischnocnema (Anura : Brachycephalidae) from Southeastern Brazil and their phylogenetic position within the I . guentheri series. Herpetological Monographs, 32, 1–21.

    https://doi.org/10.1655/HERPMONOGRAPHS-D-16-00014.1

    Taucce, P.P.G., Canedo, C., Parreiras, J.S., Drummond, L.O., Nogueira-Costa, P. & Haddad, C.F.B. (2018b) Molecular phylogeny of Ischnocnema (Anura: Brachycephalidae) with the redefinition of its series and the description of two new species. Molecular Phylogenetics and Evolution, 128, 123–146.

    https://doi.org/10.1016/j.ympev.2018.06.042

    Vences, M., Thomas, M., van der Meijden, A., Chiari, Y. & Vieites, D.R. (2005) Comparative performance of the 16S rRNA gene in DNA barcoding of amphibians. Frontiers in zoology, 2, 5.

    https://doi.org/10.1186/1742-9994-2-5