Abstract
We diagnose and describe two Songthela and one Vinathela species of the primitively segmented spiders, Liphistiidae, as new to science based on morphological characters and molecular data: Songthela pyriformis sp. nov. (male, female), Songthela shuyuan sp. nov. (male, female), Vinathela nenglianggu sp. nov. (male, female), which were collected at Mt Yuelu in Changsha, Hunan Province, China. To facilitate future identification of our species, we also provide the GenBank accession numbers of the DNA barcode gene, cytochrome c oxidase subunit I (COI) for all type specimens and the evidence of genetic distances based on COI for three new species.
References
Bristowe, W.S. (1975) A family of living fossil spiders. Endeavour, 34, 115–117.
https://doi.org/10.1016/0160-9327(75)90130-1
Brower, A.V.Z. (2010) Alleviating the taxonomic impediment of DNA barcoding and setting a bad precedent: names for ten species of ‘Astraptes fulgerator’ (Lepidoptera: Hesperiidae: Eudaminae) with DNA-based diagnoses. Systematics and Biodiversity, 8, 485–491.
https://doi.org/10.1080/14772000.2010.534512
Chen, Z.F., Zhang, Z.H. & Zhu, C.D. (1981) A new species of genus Heptathela. Journal of Hangzhou University, Natural Science Edition, 8, 305–308.
Cook, L.G., Edwards, R.D., Crisp, M.D. & Hardy, N.B. (2010) Need morphology always be required for new species descriptions? Invertebrate Systematics, 24, 322–326.
https://doi.org/10.1071/IS10011
Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3, 294–299.
Haupt, J. (1983) Vergleichende Morphologie der Genitalorgane und Phylogenie der liphistiomorphen Webspinnen (Araneae: Mesothelae). I. Revision der bisher bekannten Arten. Zeitschrift für Zoologische Systematik und Evolutionsforschung, 21, 275–293.
https://doi.org/10.1111/j.1439-0469.1983.tb00296.x
Haupt, J. (2003) The Mesothelae –monograph of an exceptional group of spiders (Araneae: Mesothelae) (Morphology, behaviour, ecology, taxonomy, distribution and phylogeny). Zoological, 154, 1–102.
Kishida, K. (1923) Heptathela, a new genus of liphistiid spiders. Annotationes Zoologicae Japonenses, 10, 235–242.
Ono, H. (1999) Spiders of the genus Heptathela (Araneae, Liphistiidae) from Vietnam, with notes on their natural history. Journal of Arachnology, 27, 37–43.
Ono, H. (2000) Zoogeographic and taxonomic notes on spiders of the subfamily Heptathelinae (Araneae, Mesothelae, Liphistiidae). Memoirs of the National Science Museum Tokyo, Series A, Zoology, 33, 145–151.
Planas, E. & Ribera, C. (2015) Description of six new species of Loxosceles (Araneae: Sicariidae) endemic to the Canary Islands and the utility of DNA barcoding for their fast and accurate identification. Zoological Journal of the Linnean Society, 174, 47–73.
https://doi.org/10.1111/zoj.12226
Schiödte, J.C. (1849) Om en afvigende Slægt af Spindlernes Orden. Naturhistorisk Tidsskrift, 2, 617–624.
Schwendinger, P.J. & Ono, H. (2011) On two Heptathela species from southern Vietnam, with a discussion of copulatory organs and systematics of the Liphistiidae (Araneae: Mesothelae). Revue suisse de Zoologie, 118, 599–637.
https://doi.org/10.5962/bhl.part.117818
Song, D.X. & Haupt, J. (1984) Comparative morphology and phylogeny of liphistiomorph spiders (Araneae: Mesothelae). 2. Revision of new Chinese heptathelid species. Verhandlungen des Naturwissenschaftlichen Vereins in Hamburg, 27, 443–451.
Song, D.X. & Hu, J.Y. (1997) On a new species of the genus Heptathela (Araneae: Liphistiidae) from Hong Kong, China. Chinese Journal of Zoology, 32 (3), 1–3.
Tamura, K., Stecher, G., Peterson, D., Filipski, A., Kumar, S. (2013) MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30 (12), 2725–2729.
https://doi.org/10.1093/molbev/mst197
Wang, J.F. & Ye, H.Q. (1983) A new species of the spider family Heptathelidae from China. Acta Zootaxonomica Sinica, 8, 146–148.
World Spider Catalog (2019) World Spider Catalog. Version 20.0. Natural History Museum, Bern. http://wsc.nmbe.ch (accessed on 31 August 2019)
Xu, X., Liu, F.X., Cheng, R.C., Chen, J., Xu, X., Zhang, Z.S., Ono, H., Pham, D.S., Norma-Rashid, Y., Arnedo, M.A., Kuntner, M. & Li, D. (2015a) Extant primitively segmented spiders have recently diversified from an ancient lineage. Proceedings of the Royal Society of London, B: Biological Sciences, 282, 20142486.
https://doi.org/10.1098/rspb.2014.2486
Xu, X., Liu, F.X., Chen, J., Ono, H., Li, D. & Kuntner, M. (2015b) A genus-level taxonomic review of primitively segmented spiders (Mesothelae, Liphistiidae). ZooKeys, 488, 121–151.
https://doi.org/10.3897/zookeys.488.8726
Xu, X., Liu, F.X., Chen, J., Li, D. & Kuntner, M. (2015c) Integrative taxonomy of the primitively segmented spider genus Ganthela (Araneae: Mesothelae: Liphistiidae): DNA barcoding gap agrees with morphology. Zoological Journal of the Linnean Society, 175 (2), 288–306.
https://doi.org/10.1111/zoj.12280
Xu, X., Liu, F.X., Chen, J., Ono, H., Li, D. & Kuntner, M. (2016) Pre-Pleistocene geological events shaping diversification and distribution of primitively segmented spiders on East Asian Margins. Journal of Biogeography, 43, 1004–1019.
https://doi.org/10.1111/jbi.12687
Xu, X., Liu, F., Ono, H., Chen, J., Kuntner, M. & Li, D. (2017) Targeted sampling in Ryukyus facilitates species delimitation of the primitively segmented spider genus Ryuthela (Araneae: Mesothelae: Liphistiidae). Zoological Journal of the Linnean Society, 181, 867–909.
https://doi.org/10.1093/zoolinnean/zlx024
Yin, C.M. (2001) A new species of the genus Heptathela and its variant type from China (Araneae: Liphistiidae). Acta Zootaxonomica Sinica, 26, 297–300.
Yin, C.M., Tang, G. & Xu, X. (2003) Two new species of the genus Heptathela from China (Araneae: Liphistiidae). Acta Arachnologica Sinica, 12, 1–5.
Yu, H., Li, F. & Zhang, J.S. (2018) A new species of the genus Songthela from Guizhou Province, China (Araneae: Mesothelae: Liphistiidae). Turkish Journal of Zoology, 42 (6), 715–721.
https://doi.org/10.3906/zoo-1807-39