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Type: Article
Published: 2019-10-01
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A new species of Megophrys Kuhl & Van Hasselt (Amphibia: Megophryidae) from Borneo allied to M. nasuta (Schlegel, 1858)

Graduate School of Global Environmental Studies, Kyoto University, Yoshida-Honmachi, Sakyo-ku, Kyoto, 606-8501, JAPAN Study Group of Wildlife and Habitat Conservation (Green Community), Department Biology, Faculty of Mathematic and Natural Sciences, Semarang State University, Semarang, Central Java, INDONESIA
Museum Zoologicum Bogoriense, Research Centre for Biology, Indonesian Institute of Sciences, Gd. Widyasatwaloka, Jl. Raya Jakarta-Bogor Km 46, Cibinong, West Java, INDONESIA
Graduate School of Human and Environmental Studies, Kyoto University, Sakyo-ku, Kyoto 606–8501, JAPAN
School of Life Science and Technology, Institut Teknologi Bandung, Labtex XI Building, 10 Jalan Ganesa, Bandung 40132, West Java, INDONESIA
Museum Zoologicum Bogoriense, Research Centre for Biology, Indonesian Institute of Sciences, Gd. Widyasatwaloka, Jl. Raya Jakarta-Bogor Km 46, Cibinong, West Java, INDONESIA
Faculty of Education, Aichi University of Education, Kariya, Aichi 448-8542, JAPAN
Amphibia Megophrys kalimantanensis sp. nov. molecular phylogeny new species taxonomy Southeast Asia

Abstract

Within Sundaland, the species diversity of the genus Megophrys is richest in Borneo, with some species restricted to the northern highlands of the island. On the basis of molecular, morphological and acoustic evidence, we describe a new species of large-sized Megophrys, once confused with M. nasuta. The new species Megophrys kalimantanensis sp. nov. can be differentiated from all its congeners by large genetic distance more than 6.11% in the mitochondrial 16S rRNA gene, much shorter rostral and orbital appendages, and unique advertisement call. This new species has been recorded from medium to high altitudes of mountain forest in southern and northernmost Borneo.

 

References

  1. Berry, P.Y. (1975) The Amphibia Fauna of Peninsular Malaysia. Tropical Press, Kuala Lumpur, x + 130 pp.

    Bioacoustics Research Program (2014) Raven Pro: Interactive sound analysis software. Version 1.5. Computer software. The Cornell Lab of Ornithology, Ithaca, New York. Available from: http://www.birds.cornell.edu/raven (accessed 20 August 2019.

    Boulenger, G.A. (1882) Catalogue of the Batrachia Salientia s. Ecaudata in the Collection of the British Museum. 2nd Edition. Taylor and Francis, London, xvi + 588 pp.

    https://doi.org/10.5962/bhl.title.20903

    Boulenger, G.A. (1885) Description of a new frog of the genus Megalophrys. Proceedings of the scientific meeting of the Zoological Society of London, 53 (4), 850–850.

    https://doi.org/10.1111/j.1096-3642.1885.tb02927.x

    Boulenger, G.A. (1899) Description of three new reptiles and a new batrachian from Mount Kinabalu, North Borneo. Annals and Magazine of Natural History, Series 7, 4 (24), 451−454.

    https://doi.org/10.1080/00222939908678228

    Boulenger, G.A. (1903) Report on the batrachians and reptiles. In: Annandale, N. & Robinson, H.C. (Ed.), Fasciculi Malayenses. Zoology. Part I. Anthropological and Zoological Results of an Expedition to Perak and the Siamese Malay States 1901–1902. Longmans, Green & Co, London, pp. 131–176.

    https://doi.org/10.5962/bhl.title.98554

    Brown, R.M., Siler, C.D., Diesmos, A.C. & Alcala, A.C. (2009) Philippine frogs of the genus Leptobrachium (Anura; Megophryidae): Phylogeny-based species delimitation, taxonomic review, and description of three new species. Herpetological Monographs, 23, 1–44.

    https://doi.org/10.1655/09-037.1

    Chen, J.-M., Zhou, W.-W., Poryakov, N.A., Stuart, B.L., Brown, R.F., Lathrop, A., Wang, Y.-Y., Yuan, Z.-Y., Jiang, K., Hou, M., Chen, H.-M., Suwannapoom, C., Nguyen S.N., Duong, T.V., Papenfuss, T.J., Murphy, R.W., Zhang, Y.P. & Che, J. (2017) A novel multilocus phylogenetic estimation reveals unrecognized diversity in Asian horned toads, genus Megophrys sensu lato (Anura, Megophryidae). Molecular Phylogenetics and Evolution, 106, 28–43.

    https://doi.org/10.1016/j.ympev.2016.09.004

    Das, I. (2006) Crocker Range National Park, Sabah, as refuge for Bornoe’s montane herpetofauna. Amphibian and Reptile Conservation, 4, 311.

    Das, I. (2007) Amphibians and Reptiles of Brunei, A Pocket Guide. Natural History Publications, Kota Kinabalu (Borneo), vii + 93 pp.

    Das, I., Jankowski, A., Makmor, M.I.B. & Haas, A. (2007) Species diversity, elevational distribution and reproductive modes in an amphibian community at the Matang Range, Sarawak (Borneo). Mitteilungen des hamburger Zoologischen Museums und Instituts, 104, 141174.

    Dehling, J.M. (2015) A new species of Rhacophorus (Anura, Rhacophoridae) from Gunung Kinabalu, Borneo. Salamandra, 51, 1–11.

    Dehling, J.M., Matsui, M. & Yambun, P. (2016) A new small montane species of Philautus (Amphibia, Anura, Rhacophoridae) from Gunung Kinabalu, Sabah, Malaysia (Borneo). Salamandra, 52, 77–90.

    Delorme, M., Dubois, A., Grosjean, S. & Ohler, A. (2006) Une nouvelle ergotaxinomie des Megophryidae (Amphibia, Anura). Alytes, 24, 6−21.

    Dubois, A. & Ohler, A. (1998) A new species of Leptobrachium (Vibrissaphora) from northern Vietnam, with a review of the taxonomy of the genus Leptobrachium (Pelobatidae, Megophryinae). Dumerilia, 4 (1), 1–32.

    Edeling, A.C.J. (1864) Description d’une nouvelle espèce du genre Megalophrys, le Megalophrys chysii. Natuurkundig Tijdschrift voor Nederlandsch Indië, 27, 265–267.

    Eprilurahman, R., Yudha, D.S., Kusuma, K.I. & Surya, P.A. (2012) Kenakeragaman Amfibi di Kalimantan. In: Susandarini, R., Purnomo, Sancayaningsih, R.P., Astuti, I.P., Sari, R., Irawati, Yudha, D.S., Eprilurahman, R., Trijoko, Hadisusanto, S., Hanifa, B.F., Khoirizzaad, I., Huda, M., Bait, M., Surya, P.A., Ambar, W.R. & Putra, Y.R. (Eds.), Flora dan Fauna Kalimantan, Dokumentasi Hasil Tim Peneliti Ekspedisi Katulistiwa. Fakultas Biologi Universitas Gajah Mada, Yogyakarta, pp. i–viii + 1–155.

    Eto, K., Matsui, M. & Nishikawa, K. (2015) Description of a new species of the genus Leptobrachella (Amphibia, Anura, Megophryidae) from Borneo. Current Herpetology, 34 (2), 128–139.

    https://doi.org/10.5358/hsj.34.128

    Eto, K., Matsui, M. & Nishikawa, K. (2016) A new highland species of dwarf litter frog genus Leptobrachella (Amphibia, Anura, Megophryidae) from Sarawak. Raffles Bulletin of Zoology, 64, 194–203.

    Eto, K., Matsui, M., Hamidy, A., Munir, M. & Iskandar, D.T. (2018) Two new species of the genus Leptobrachella (Amphibia: Anura: Megophryidae) from Kalimantan, Indonesia. Current Herpetology, 37 (2), 95–105.

    https://doi.org/10.5358/hsj.37.95

    Foquet, A., Gilles, A., Vences, M., Marty, C., Blanc, M. & Gemmel, N.J. (2007) Underestimation of species richness in Neotropical frog revealed by mtDNA analyses. PLoSONE, 2 (10), 1–10.

    https://doi.org/10.1371/journal.pone.0001109

    Frost, D.R. (2019) Amphibian Species of the World, an Online Reference. Version 6.0. Electronic Database, American Museum of Natural History, New York, USA. Available from: http//research.amnh.org/herpetology/amphibia/index.html (accessed 2 May 2019)

    Frost, D.R., Grant, T., Faivovich, J., Bain, R.H., Haas, A., Haddad, C.F.B., de Sá, R.O., Channing, A., Wilkinson, M., Donnellan, S.C., Raxworthy, C.J., Campbell, J.A. Blotto, B.L., Moler, P.E., Drewes, R.C., Nussbaum, R.A., Lynch, J.D., Green, D.M. & Wheeler, W.C. (2006) The amphibian tree of life. Bulletin of the American Museum of Natural History, 297, 1–370.

    https://doi.org/10.1206/0003-0090(2006)297[0001:TATOL]2.0.CO;2

    Gosner, K.L. (1960) A simplified table for staging anuran embryos and larvae with notes on identification. Herpetologica, 16 (3), 183–190.

    Graves, E.E. (1981) The Minangkabau response to Dutch colonial rule in the nineteenth century. Cornell Southeast Asia Program Publication, New York, vii + 147 pp.

    Haas, A. & Das, I. (2011) Describing east Malaysian tadpole diversity: status and recommendation for standard and procedures associated with larval amphibian description and documentation. Bonner Zoologische Monographien, 57, 29–46.

    Hamidy, A., Matsui, M., Shimada, T., Nishikawa, K., Yambun, P., Sudin, A., Kusrini, M.D. & Kurniati, H. (2011) Morphological and genetic discordance in two species of Bornean Leptobrachium (Amphibia, Anura, Megophryidae). Molecular Phylogenetic and Evolution, 61, 904913.

    https://doi.org/10.1016/j.ympev.2011.08.020

    Hamidy, A., Matsui, M., Nishikawa, K. & Belabut, D.M. (2012) Detection of cryptic taxa in Leptobrachium nigrops (Amphibia, Anura, Megophryidae), with description of two new species. Zootaxa, 3398 (1), 2239.

    https://doi.org/10.11646/zootaxa.3398.1

    Hamidy, A. & Kurniati, H. (2015) A new species of tree frog genus Rhacophorus from Sumatra, Indonesia (Amphibia, Anura). Zootaxa, 3947 (1), 49–66.

    https://doi.org/10.11646/zootaxa.3947.1.3

    Hamidy, A., Munir, M., Mumpuni, M., Rahmania, M. & Kholik, A.A. (2018) Detection cryptic taxa in the genus Leptophryne (Fitzinger, 1843) (Amphibia; Bufonidae) and the description of a new species from Java, Indonesia. Zootaxa, 4450 (4), 427−444.

    https://doi.org/10.11646/zootaxa.4450.4.2

    Hasan, M., Islam, M.M., Khan, M.R., Igawa, T., Alam, M.S., Djong, H.T., Kurniawan, N., Joshy, H., Sen,Y.H., Belabut, D.M., Kurabayashi, A., Kuramoto, M. & Sumida, M. (2014) Genetic divergences of South and Southeast Asian frogs: a case study of several taxa based on 16S ribosomal RNA gene data with notes on the generic name Fejervarya. Turkey Journal of Zoology, 38, 389−411.

    https://doi.org/10.3906/zoo-1308-36

    Hammer, O., Harper, D.A.T & Ryan, P.D. (2001) PAST: Paleontological Statistics Software Package for Education and Data Analysis. Palaeontologia Electronica, 4, 1−9.

    Howard, S.D. (2012) Ecology and conservation of poorly known herpetofaunal diversity in Borneo. Unpublished thesis, National University of Singapore, Singapore, xiii + 233 pp.

    Inger, R.F. (1954) The systematics and zoogeography of Philippine Amphibia. Fieldiana Zoology, 33 (4), 183–531.

    https://doi.org/10.5962/bhl.title.5571

    Inger, R.F. (1966) The systematics and zoogeography of the Amphibia of Borneo. Fieldiana Zoology, 52, 1–402.

    https://doi.org/10.5962/bhl.title.3147

    Inger, R.F. (1989) Four new species of frogs from Borneo. Malayan Nature Journal, 42, 229–243.

    Inger, R.F. & Stuebing, R.B. (1992) The montane amphibian fauna of north-western Borneo. Malayan Nature Journal, 46, 4151.

    Inger, R.F., Stuebing, R.B. & Tan, F.L. (1995) New species and new records of anurans from Borneo. The Raffles Bulletin of Zoology, 43, 115−131.

    Inger, R.F. & Tan, F.L. (1996) Checklist of the frogs of Borneo. The Raffles Bulletin of Zoology, 44, 551−574.

    Inger, R.F., Boeadi & Taufik, A. (1996) New species of Ranid frogs (Amphibia: Anura) from Central Kalimantan, Borneo. Raffles Bulletin of Zoology, 44 (1), 363–369.

    Iskandar, D.T. (1978) A new species of Barbourula: First record of a Discoglossid anuran in Borneo. Copeia, 4, 564–566.

    https://doi.org/10.2307/1443681

    Iskandar, D.T. (2004) The amphibians and reptiles of Malinau region, Bulungan research forest, East Kalimantan, annotated checklist with note on ecological preferences of the species and local utilization. Center for International Forestry Research (CIFOR), Bogor, v + 27 pp.

    Iskandar, D.T., Bickford, D.P. & Arifin, U. (2011) A new Ingerana (Anura, Dicroglossidae) with no external tympanum from Borneo, Indonesia. Raffles Bulletin of Zoology, Singapore, 59 (2), 213–218.

    Köhler, J., Jansen, M., Rodrĺguez, A., Kok, P.J.R., Toledo, L.P., Emmrich, M., Glaw, F., Haddad, C.F.B., Rödel, M.-O. & Vences, M. (2017) The use of bioacoustics in anuran taxonomy: theory, terminology, methods and recommendations for best practice. Zootaxa, 4251 (1), 1–124.

    https://doi.org/10.11646/zootaxa.4251.1.1

    Kurabayashi, A., Matsui, M., Belabut, D.M., Yong, H.-S., Ahmad, N., Sudin, A., Kuramoto, M., Hamidy, A. & Sumida, M. (2011) From Antartica or Asia? New colonization scenario for Australian-New Guinean narrow mouth toads suggested from the findings on a myserious genus Gastrophrynoides. BMC Evolutionary Biology, 11, 175.

    https://doi.org/10.1186/1471-2148-11-175

    Lathrop, A. (1997) Taxonomic review of the Megophryid frogs (Anura: Pelobatidae). Asiatic Herpetological Research, 7, 68–79.

    https://doi.org/10.5962/bhl.part.18857

    Mahony, S., Foley, N.M., Biju, S.D. & Teeling, E.C. (2017) Evolutionary history of the Asian horned frogs (Megophryinae), Integrative approaches to time tree dating in the absence of fossil record. Molecular Biology and Evolution, 34 (3), 744–771.

    https://doi.org/10.1093/molbev/msw267

    Malkmus, R. & Matsui, M. (1997) Megophrys kobayashii, ein neuer pelobatider frosch vom Mount Kinabalu, Sauria, 19, 31–37.

    Malkmus, R., Manthey, U., Vogel, G., Hoffmann, P. & Kosuch, J. (2002) Amphibians and Reptiles of Mount Kinabalu (North Borneo). A.R.G. Gantner Verlag Kommandit, Ruggell, Berlin, 424 pp.

    Manthey, U. & Grossmann, W. (1997) Amphibien und Repetilien Südostasiens. Natur und Tier, Verlag, Berlin, 512 pp.

    Matsui, M. (2006) Anuran inventory in Sabahpast and future. Current Herpetology, 25, 114.

    https://doi.org/10.3105/1345-5834(2006)25[1:AIISAF]2.0.CO;2

    Matsui, M., Hamidy, A., Murphy, R.W., Khonsue, W., Yambun, P., Shimada, T., Norhayatii, A., Daicus, B.M. & Jiang, J.-P. (2010) Phylogenetic relationships of Megophryid frogs of the genus Leptobrachium (Amphibia, Anura) as revealed by mtDNA gene sequences. Molecular Phylogenetic and Evolution, 56, 259–272.

    https://doi.org/10.1016/j.ympev.2010.03.014

    Matsui, M., Nishikawa, K. & Yambun, P. (2014) A new Leptolalax from mountains of Sabah, Borneo (Amphibia, Anura, Megophryidae). Zootaxa, 3753 (5), 440–452.

    https://doi.org/10.11646/zootaxa.3753.5.3

    Matsui, M. (2015) A new species of Limnonectes from the border of East Kalimantan and Sarawak, Borneo Island (Anura, Dicroglossidae). Current Herpetology, 34 (2), 120–127.

    https://doi.org/10.5358/hsj.34.120

    Matsui, M., Eto, K., Nishikawa, K., Hamidy, A., Belabut, D., Ahmad, N., Panha, S., Khonsue, W. & Grismer, L.L. (2017a) Mitochondrial phylogeny of Leptolalax from Malay Peninsula and Leptobrachella (Anura, Megophryidae). Current Herpetology, 36 (2), 1–11.

    https://doi.org/10.5358/hsj.36.11

    Matsui, M., Nishikawa, K., Eto, K. & bin Hossman, M.Y. (2017b) A New Species of Pelophryne from Western Sarawak, Malaysian Borneo (Anura, Bufonidae). Zoological Science, 34 (4), 345−350.

    https://doi.org/10.2108/zs170008

    McDiarmid, R.W. & Altig, R. (1999) Tadpoles, the biology of anuran larvae. The University of Chicago Press, Chicago, xiv + 444 pp.

    Miracle, M.E.G., van Den Hoek Ostende, L.W. & Artntzen, J.W. (2007) Type specimens of amphibians in the National Museum of Natural History, Leiden, The Netherlands. Zootaxa, 1482 (1), 25−68.

    https://doi.org/10.11646/zootaxa.1482.1.2

    Munir, M., Hamidy, A., Farajallah, A. & Smith, E.N. (2018) A new Megophrys Kuhl and van Hasselt (Amphibia: Megophryidae) from southwestern Sumatra, Indonesia. Zootaxa, 4442 (3), 389−412.

    https://doi.org/10.11646/zootaxa.4442.3.3

    Oberhummer, E., Barten, C., Schweizer, M., Das, I., Haas, A. & Hertwig, S.T. (2014) Description of the tadpoles of three rare species of megophryid frogs (Amphibia: Anura: Megophryidae) from Gunung Mulu, Sarawak, Malaysia. Zootaxa, 3835 (1), 59−79.

    https://doi.org/10.11646/zootaxa.3835.1.3

    Pistorius, A.W.P.V. (1871) Studien over de Inlandsche huishoudin in de Padangsche bovenlanden. Joh. Noman en Zoon, Zalt-Bommel, iv + 240 pp.

    Rueden, C.T., Schindelin, J., Hiner, M.C., DeZonia, B.E., Walter, A.E., Arena, E.T. & Eliceiri, K.W. (2017) ImageJ2: ImageJ for the next generation of scientific image data. BMC Bioinfomatics, 18 (1), 529

    https://doi.org/10.1186/s12859-017-1934-z

    Schlegel, H. (1858) Handleiding tot de Beoefening der Dierkunde. Vol. 2. Koninklijke Militaire Akademie, Breda, xx + 664 pp.

    https://doi.org/10.5962/bhl.title.11648

    Shimada, T., Matsui, M., Nishikawa, K. & Eto, K. (2015) A new species of Meristogenys (Anura, Ranidae) from Sarawak, Borneo. Zoological Science, 32 (5), 474–484.

    https://doi.org/10.2108/zs140289

    Smith, M.A. (1926) The function of the “funnel” mouth of the tadpoles of Megalophrys, with a note on M. aceras Boulenger. Proceeding of Zoological Society London, 96 (4), 983–988.

    https://doi.org/10.1111/j.1096-3642.1926.tb02229.x

    Taylor, E.H. (1920) Philippine Amphibia. The Philippine Journal of Science, 16 (3), 213–359.

    https://doi.org/10.5962/bhl.part.4751

    Taylor, E.H. (1962) The amphibian fauna of Thailand. The University of Kansas Science Bulletin, 42, 265−599.

    https://doi.org/10.5962/bhl.part.13347

    van Kampen, P.N. (1923) The Amphibia of the Indo−Australian Archipelago. E.J. Brill, Leiden, xii + 304 pp.

    https://doi.org/10.5962/bhl.title.12187

    Veith, M., Wulffraat, S., Kosusch, J., Hallmann, G., Henkel, H.-W., Sound, P., Samsu, Rudhimanto, L. & Iskandar, D.T. (2004) Amphibians of the Kayan Mentaran National Park (East Kalimantan, Indonesia), estimating overall and local species richness. Tropical Zoology, 17 (1), 113.

    https://doi.org/10.1080/03946975.2004.10531197

    Vences, M., Thomas, M., van der Meijden, A., Chiari, Y. & Vieites, D.R. (2005) Comparative performance of the 16S rRNA gene in DNA barcoding of amphibians. Frontiers in Zoology, 2, 5.

    https://doi.org/10.1186/1742-9994-2-5

    Waser, L.E., Schweizer, M., Haas, A., Das, I., Jankowski, A., Min, P.Y. & Hertwig, S.T. (2017) From a lost world: an integrative phylogenetic analysis of Ansonia Stoliczka, 1870 (Lissamphibia: Anura: Bufonidae), with the description of a new species. Organisms, Diversity and Evolution, 17 (1), 287–303.

    https://doi.org/10.1007/s13127-016-0294-2

    Wildenhues, M., Rauhaus, A., Bach, R., Karbe, D., van der Straten, K., Herwig, S.T. & Ziegler, T. (2012) Husbandry, captive breeding, larval development and stages of the Malayan horned frog Megophrys nasuta (Schlegel, 1858) (Amphibia: Anura: Megophryidae). Amphibian and Reptile Conservation, 5 (3), 15–28.

    Zug, G.R. (2015) Morphology and systematics of Kalophrynus interlineatuspleurostigma populations (Anura, Microhylidae, Kalophryinae) and a taxonomy of the genus Kalophrynus Tschudi, Asian sticky frogs. Proceedings of the California Academy of Sciences, 62, 135–190.