Abstract
A new spined loach of the genus Cobitis Linnaeus is described from the Buquan River, located in Guangxi Zhuang Autonomous Region, China. The species differs from other members of Cobitis in China by a combination of morphological characters, none of them unique: body depth 14.3‒18.4% SL; lamina circularis long and knife-shaped; Gambetta zones present on the dorsolateral sides of the body (L1‒L5); 8‒11 large transverse elongated blotches on L5; 9‒12 large transverse elongated blotches on L1; maxillary barbels longer than eye diameter; 4‒5 narrow rows of dark spots on the caudal fin; caudal fin with 14 branched rays; a rectangular spot slightly smaller than eye diameter on the upper side of caudal fin base; and caudal-peduncle depth 79.5‒93.1% its length.
References
Bănărescu, P. (1990) Zoogeography of Freshwaters: General Distribution and Dispersal of Freshwater Animals. Aula-Verlag, Wiesbaden, 688 pp.
Bohlen, J. & Harant, R. (2010) Microcobitis, a new genus name for Cobitis misgurnoides (Teleostei: Cobitidae). Ichthyological Exploration of Freshwaters, 21, 295–300.
Chen, J.X. & Zhu, S.Q. (1984) Phylogenetic relationships of the subfamilies in the loach family Cobitidae (Pisces). Acta Zootaxonomica Sinica, 9 (2), 201–208.
Chen, Y.F. & Chen, Y.X. (2005) Secondary sexual characters, pigmentary zones of Gambetta and taxonomical revision the genus Cobitis from China (Pisces: Cobitidae: Cobitinae). Acta Zootaxonomica Sinica, 30 (4), 647–658.
Chen, Y.X. & Chen, Y.F. (2011) Two new species of cobitid fish (Teleostei, Cobitidae) from the River Nanliu and River Beiliu, China. Folia Zoologica, 60 (2), 143–152.
https://doi.org/10.25225/fozo.v60.i2.a8.2011
Chen, Y.X. & Chen, Y.F. (2013) Three new species of cobitid fish (Teleostei, Cobitidae) from the River Xinjiang and the River Le’anjiang, tributaries of the Lake Poyang of China, with remarks on their classification. Folia Zoologica, 62 (2), 83–95.
https://doi.org/10.25225/fozo.v62.i2.a1.2013
Chen, Y.X. & Chen, Y.F. (2016) A new species of the genus Cobitis (Cypriniformes: Cobitidae) from the Northeast China. Zoological Systematics, 41 (4), 379–391.
Chen, Y.X., Chen, Y.F. & He, D.K. (2013) A new species of spined loach (Osteichthyes, Cobitidae) from the Pearl River, Guangxi of China. Acta Zootaxonomica Sinica, 38 (2), 377–387.
Chen, Y., He, D., Chen, H. & Chen, Y. (2017) Taxonomic study of the genus Niwaella (cypriniformes: cobitidae) from east china, with description of four new species. Zoological Systematics, 42 (4), 490–507.
Chen, Y.X., Sui, X.Y., He, D.K. & Chen, Y.F. (2015) Three new species of cobitid fishes genus Cobitis (Teleostei, Cobitidae) from the River Pearl basin of China. Folia Zoologica, 64 (1), 1–16.
Chen, Y.X., Sui, X.Y., Liang, N. & Chen, Y.F. (2016) Two new species of the genus Cobitis Linneaus (Teleostei: Cobitidae) from southern China. Chinese Journal of Oceanology and Limnology, 34 (3), 517–525.
https://doi.org/10.1007/s00343-016-4277-0
Freyhof, J. & Serov, D.V. (2000) Cobitis laoensis from Vietnam with notes on the southern distribution limits of Indochinese Cobitis (Teleostei: Cobitidae). Folia Zoologica, 49 (Supplement 1), 205–214.
Gambetta, L. (1934) Sulla variabilita del cobite fluviale (Cobitis taenia L.) e sul rapporto numerico dei sessi. Boll Mus Zool Anat Comp R Univ Torino, 44, 297–324.
Huelsenbeck, J.P. & Ronquist, F. (2001) MrBayes: Bayesian inference of phylogeny. Bioinformatics, 17, 754–755.
https://doi.org/10.1093/bioinformatics/17.8.754
Kim, K.Y., Lee, S.Y., Bang, I.C. & Nam, Y.K. (2008) Complete mitogenome sequence of an endangered freshwater fish, Iksookimia choii (Teleostei; Cypriniformes; Cobitidae). Mitochondrial DNA, 19, 438–445.
Kottelat, M. (1984) A review of the species of Indochinese fresh-water fishes described by H. E. Sauvage. Bulletin du Museum National d'Histoire Naturelle, Série 4: Section A: Zoologie, Biologie et Écologie Animales, 6 (3), 791–822.
Kottelat, M. (2012) Conspectus Cobitidum: an inventory of the loaches of the world (Teleostei: Cypriniformes: Cobitoidei). Raffles Bulletin of Zoology, 26 (Supplement), 1–199.
Kottelat, M. & Freyhof, J. (2007) Handbook of European freshwater fishes. Publications Kottelat, Cornol, 646 pp.
Kumar, S., Stecher, G. & Tamura, K. (2016) MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Molecular Biology and Evolution, 33 (7), 1870.
Perdices, A., Bohlen, J., Šlechtová, V. & Doadrio, I. (2016) Molecular evidence for multiple origins of the european spined loaches (teleostei, cobitidae). Plos One, 11 (1), e0144628.
https://doi.org/10.1371/journal.pone.0144628
Perdices, A. & Doadrio, I. (2001) The molecular systematics and biogeography of the European cobitids based on mitochondrial DNA sequences. Molecular Phylogenetics and Evolution, 19 (3), 468–478.
https://doi.org/10.1006/mpev.2000.0900
Posada, D. & Crandall, K.A. (1998) MODELTEST: testing the model of DNA substitution. Bioinformatics, 14, 817–818.
https://doi.org/10.1093/bioinformatics/14.9.817
Sauvage, H.E. (1878) Note sur quelques poissons d'espèces nouvelles provenant des eaux douces de l'Indo-Chine. Bulletin de la Société philomathique de Paris, 7 (2), 233–242.
Sambrook, J. & Russell, D.W. (2001) Molecular Cloning: a Laboratory Manual. Cold Spring Harbor Press, New York, 2100 pp.
Šlechtová, V., Bohlen, J. & Perdices, A. (2008) Molecular phylogeny of the freshwater fish family Cobitidae (Cyriniformes: Teleostei): delimitation of genera, mitochondrial infrogression and evolution of sexual dimorphism. Molecular Biology & Evolution, 47, 812–831.
Stamatakis, A. (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics, 30 (9), 1312–1313.
https://doi.org/10.1093/bioinformatics/btu033
Son, Y.M. & He, S.P. (2005) Cobitis zhejiangensis, a new species from the Ling River, China (Teleostei: Cobitidae). Korean Journal of Ichthyology, 17 (4), 236–240.
Takeda, R. & Fujie, K. (1945) Distribution of some color pattern types Cobitis taenia. Zoology Magnetism, 56, 1–5.
Tang, Q., Liu H., Mayden, R. & Xiong, B. (2006) Comparison of evolutionary rates in the mitochondrial dna cytochrome b gene and control region and their implications for phylogeny of the cobitoidea (teleostei : cypriniformes). Molecular Phylogenetics & Evolution, 39 (2), 347–357.
https://doi.org/10.1016/j.ympev.2005.08.007
Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. & Higgins, D.G. (1997) The clustal X windown interface: flexible strategies for multiple sequences alignment aided by quality analysis tools. Nucleic Acids Research, 25 (25), 4876–4882.
https://doi.org/10.1093/nar/25.24.4876
Xiao, W.H., Zhang, Y.P. & Liu, H.Z. (2001) Molecular systematics of Xenocyprinae (Teleostei: Cyprinidae): taxonomy, biogeography, and coevolution of a special group restricted in East Asia. Molecular Phylogenetics and Evolution, 18 (2), 163–173.