Abstract
Two new species of the gekkonid lizard genus Hemiphyllodactylus are described based on specimens collected from Champasak Province in southern Laos and Houaphanh Province in northern Laos. Phylogenetic analyses recover H. indosobrinus sp. nov. from Champasak Province as the sister lineage to the Thai endemic H. flaviventris. Hemiphyllodactylus indosobrinus sp. nov. can be diagnosed by having 15 supralabial scales, eight chin scales, 4‑5‑5‑4 subdigital lamellae on Fingers II–V, 4‑5‑5‑5 subdigital lamellae on Toes II–V, and 18 continuous precloacofemoral pores. Phylogenetic analyses recover H. serpispecus sp. nov. from Houaphanh Province to be most closely related to the Vietnamese endemic H. banaensis. Hemiphyllodactylus serpispecus sp. nov. differs from congeners by having seven chin scales; 3‑4‑4‑4 subdigital lamellae on Fingers II–V; 3‑4‑4‑5 subdigital lamellae on Toes II–V; and 11 continuous precloacofemoral pores. The recognition of these two new species doubles the number of Hemiphyllodactylus known from Laos.
References
Bain, R.H. & Hurley, M.M. (2011) A biogeographic synthesis of the amphibians and reptiles of Indochina. Bulletin of the American Museum of Natural History, 360, 1–138.
Beddome, R.H. (1870) Descriptions of some new Lizards from the Madras Presidency, with 2 Plates. Madras Monthly Journal of Medical Science, 1, 30–35.
Bleeker, P. (1860) Reptilien van Agam. Natuurkundig Tijdschrift voor Nederlandsch Indie, 20, 325–329.
Boulenger, G.A. (1903) XLI.—Descriptions of new lizards in the collection of the British Museum. Annals and Magazine of Natural History, Series 7, 12, 429–435.
https://doi.org/10.1080/00222930308678877
Cobos, A., Grismer, L.L., Wood Jr., P.L., Quah, E.S.H., Anuar, S. & Muin, M.A. (2016) Phylogenetic relationships of geckos of the Hemiphyllodactylus harterti group, a new species from Penang Island, Peninsular Malaysia, and a likely case of true cryptic speciation. Zootaxa, 4107 (3), 367–380.
http://doi.org/10.11646/zootaxa.4107.3.5
Duméril, A.M.C. & Bibron, G. (1836) Erpetologie Générale ou Histoire Naturelle Complete des Reptiles. Vol. 3. Roret, Paris, 528 pp.
https://doi.org/10.5962/bhl.title.45973
Erixon, P., Svennblad, B., Britton, T. & Oxelman, B. (2003) Reliability of Bayesian posterior probabilities and bootstrap frequencies in phylogenetics. Systematic Biology, 52, 665–673.
Gamble, T., Greenbaum, E., Jackman, T.R., Russell, A.P. & Bauer, A.M. (2012) Repeated origin and loss of adhesive toepads in geckos. PLoS ONE, 7, e39429.
https://doi.org/10.1371/journal.pone.0039429
Grismer, L.L., Wood Jr., P.L., Anuar, S., Muin, M.A., Quah, E.S.H., McGuire, J.A., Brown, R.M., Tri, N.V. & Pham, T.H. (2013) Integrative taxonomy uncovers high levels of cryptic species diversity in Hemiphyllodactylus Bleeker, 1860 (Squamata: Gekkonidae) and the description of a new species from Peninsular Malaysia. Zoological Journal of the Linnean Society, 169, 849–880.
https://doi.org/10.1111/zoj.12064
Grismer, L.L., Riyanto, A., Iskandar, D.T. & McGuire, J.A. (2014a) A new species of Hemiphyllodactylus Bleeker, 1860 (Squamata: Gekkonidae) from Pulau Enggano, southwestern Sumatra, Indonesia. Zootaxa, 3821 (4), 485–495.
https://doi.org/10.11646/zootaxa.3821.4.7
Grismer, L.L., Wood Jr., P.L. & Cota, M. (2014b) A new species of Hemiphyllodactylus Bleeker, 1860 (Squamata: Gekkonidae) from northwestern Thailand. Zootaxa, 3760 (1), 67–78.
https://doi.org/10.11646/zootaxa.3760.1.4
Grismer, L.L., Wood Jr., P.L., Anuar, S., Quah, E.S.H., Muin, M.A., Chan, K.O., Sumarli, A.X. & Loredo, A.I. (2015) Repeated evolution of sympatric, palaeoendemic species in closely related, co‐distributed lineages of Hemiphyllodactylus Bleeker, 1860 (Squamata: Gekkonidae) across a sky‐island archipelago in Peninsular Malaysia. Zoological Journal of the Linnean Society, 174, 859–876.
https://doi.org/10.1111/zoj.12254
Grismer, L.L., Wood Jr., P.L., Thura, M.K., Zin, T., Quah, E.S., Murdoch, M.L., Grismer, M.S., Li, A., Kyaw, H. & Lwin, N. (2017) Phylogenetic taxonomy of Hemiphyllodactylus Bleeker, 1860 (Squamata: Gekkonidae) with descriptions of three new species from Myanmar. Journal of Natural History, 52, 881–915.
https://doi.org/10.1080/00222933.2017.1367045
Guo, W., Zhou, K., Yan, J. & Li, P. (2015) A new species of Hemiphyllodactylus Bleeker, 1860 (Squamata: Gekkonidae) from western Yunnan, China. Zootaxa, 3974 (3), 377–390.
https://doi.org/10.11646/zootaxa.3974.3.5
Heinicke, M.P., Greenbaum, E., Jackman, T.R. & Bauer, A.M. (2011) Phylogeny of a trans-Wallacean radiation (Squamata, Gekkonidae, Gehyra) supports a single early colonization of Australia. Zoologica Scripta, 40, 584–602.
https://doi.org/10.1111/j.1463-6409.2011.00495.x
Katoh, K. & Standley, D.M. (2013) MAFFT Multiple Sequence Alignment software version 7: improvements in performance and useability. Molecular Biology and Evolution, 30, 772–780.
https://doi.org/10.1093/molbev/mst010
Köhler, G. (2012) Color Catalogue for Field Biologists. Herpeton, Offenbach, 300 color swatches, 49 pp.
Lanfear, R., Calcott, B., Ho, S.Y.W. & Guindon, S. (2012) PartitionFinder: combined selection of partitioning schemes and substitution models for phylogenetic analyses. Molecular Biology and Evolution, 29, 1695–1701.
https://doi.org/10.1093/molbev/mss020
Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. 2010 Gateway Computing Environments Workshop (GCE), New Orleans, Louisiana, 14 November 2010, 1–8.
https://doi.org/10.1109/GCE.2010.5676129
Ngo, T. V., Grismer, L.L., Thai, P.H. & Wood Jr., P.L. (2014) A new species of Hemiphyllodactylus Bleeker, 1860 (Squamata: Gekkonidae) from Ba Na–Nui Chua Nature Reserve, Central Vietnam. Zootaxa, 3760 (4), 539–552.
https://doi.org/10.11646/zootaxa.3760.4.3
Nguyen, T.Q., Lehmann, T., Le, M.D., Duong, H.T., Bonkowski, M. & Ziegler, T. (2013) A new species of Hemiphyllodactylus (Reptilia: Gekkonidae) from northern Vietnam. Zootaxa, 3736 (1), 89–98.
https://doi.org/10.11646/zootaxa.3736.1.5
Nguyen, T.Q., Botov, A., Le, M.D., Nophaseud, L., Zug, G., Bonkowski, M. & Ziegler, T. (2014) A new species of Hemiphyllodactylus (Reptilia: Gekkonidae) from northern Laos. Zootaxa, 3827 (1), 45–56.
https://doi.org/10.11646/zootaxa.3827.1.4
Rambaut A., Suchard, M.A., Xie, D. & Drummond, A.J. (2014) Tracer. Version 1.6. Available from: http://tree.bio.ed.ac.uk/software/tracer/ (accessed 9 July 2018)
Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model spaces. Systematic Biology, 61, 1–4.
https://doi.org/10.1093/sysbio/sys029
Sukprasert, A., Sutthiwises, S., Lauhachinda, V. & Taksintum, W. (2018) Two new species of Hemiphyllodactylus Bleeker (Squamata: Gekkonidae) from Thailand. Zootaxa, 4369 (3), 363–376.
https://doi.org/10.11646/zootaxa.4369.3.4
Swofford, D.L. (2002) PAUP*: (Phylogenetic Analysis Using Parsimony* and other methods). Version 4.0b10. Sinauer Associates, Sunderland, Massachusetts, USA. Available from: http://paup.phylosolutions.com (accessed 9 July 2018)
Yan, J., Lin, Y., Guo, W., Li, P. & Zhou, K. (2016) A new species of Hemiphyllodactylus Bleeker, 1860 (Squamata: Gekkonidae) from Guizhou, China. Zootaxa, 4117 (4), 543–554.
https://doi.org/10.11646/zootaxa.4117.4.6
Zhou, K.-Y. & Liu, Y.-Z. (1981) Three new subspecies of Hemiphyllodactylus yunnanensis (Boulenger) from China (Lacertiformes: Gekkonidae). Ota, H. (translator). 1996. In: Zhou, K.-Y., Liu, Y.-Z. & Yang, G.-P. (Eds.), Smithsonian Herpetological Information Service, 110, pp. 2–6.
https://doi.org/10.5479/si.23317515.110.1
Zug, G.R. (2010) Speciation and dispersal in a low diversity taxon: the slender geckos Hemiphyllodactylus (Reptilia, Gekkonidae). Smithsonian Contributions to Zoology, 631, 1–70.
https://doi.org/10.5479/si.00810282.631
Zug, G.R., Watling, D., Alefaio, T., Alefaio, S. & Ludescher, C. (2003) A new gecko (Reptilia: Squamata: genus Lepidodactylus) from Tuvalu, south-central Pacific. Proceedings of the Biological Society of Washington, 116, 38–46.