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Type: Article
Published: 2019-02-28
Page range: 67–82
Abstract views: 112
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A new species of Leptobrachella (Anura: Megophryidae) from southern Guangxi, China

Key Laboratory of Beibu Gulf Environment Change and Resources Utilization of Ministry of Education, Nanning Normal University, Nanning 530001, China Guangxi Key Laboratory of Earth Surface Processes and Intelligent Simulation, Nanning Normal University, Nanning 530001, China
Natural History Museum of Guangxi, Nanning 530012, China
Natural History Museum of Guangxi, Nanning 530012, China
Natural History Museum of Guangxi, Nanning 530012, China
Amphibia Acoustics Leptobrachella shangsiensis sp. nov. morphology Shiwandashan National Nature Reserve

Abstract

This study describes a new member of the genus Leptobrachella, Leptobrachella shangsiensis sp. nov., based on morphological, acoustic and molecular analyses from southern Guangxi, China. L. shangsiensis sp. nov. differs from its congeners by a combination of the following characters: (1) SVL 24.9–29.4 mm in 11 males and 30.8–35.9 mm in 5 females, (2) black supratympanic fold with dark reddish rim, (3) ventrolateral glands distinct, forming a broken line, (4) ventral surface yellowish creamy-white with marble texture, (5) iris copper in the upper and silver in the lower fifth and (6) toe webbing basal and narrow lateral fringes. The male advertisement call of L. shangsiensis sp. nov. consists of one note per call, lacking an introductory note, with a mean dominant frequency of 5.5–6.5 kHz at the ambient temperature of 21.5ºC. Uncorrected sequence divergences between L. shangsiensis sp. nov. and all homologous DNA sequences available for the 16S rRNA gene were found to be > 5.9%. L. shangsiensis sp. nov. occurs in an evergreen forest in Shiwandashan National Nature Reserve located at an elevation of 450–550 m.

 

References

  1. Altschul, S.F., Madden, T.L, Schäffer, A.A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D.J. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research, 25, 3389–3402.

    https://doi.org/10.1093/nar/25.17.3389

    AmphibiaChina (2018) The database of Chinese amphibians. Kunming Institute of Zoology (CAS), Kunming, Yunnan, China. Availabe from: http://www.amphibiachina.org/ (accessed October 24, 2018)

    Anderson, J. (1871) A list of the reptilian accession to the Indian Museum, Calcutta, from 1865 to 1870, with a description of some new species. Journal of the Asiatic Society of Bengal, 40, 12–39.

    Boulenger, G.A. (1893) Concluding report on the reptiles and batrachians obtained in Burma by Signor L. Fea, dealing with the collection made in Pegu and the Karin Hills in 1887–88. Annali del Museuo Civico di Genoa, 13, 304–347.

    Boulenger, G.A. (1900) Descriptions of new batrachians and reptiles from the Larut Hills, Perak. Annals and Magazine of Natural History, Series 7, 6, 186–194.

    https://doi.org/10.1080/00222930008678356

    Boulenger, G.A. (1908) A revision of the Oriental pelobatid batrachians (genus Megalophrys). Proceedings of the Zoological Society of London, 1908, 407–430.

    https://doi.org/10.1111/j.1096-3642.1908.tb01852.x

    Chen, J.M., Poyarkov, N.A. Jr., Suwannapoom, C., Lathrop, A., Wu, Y.H., Zhou, W.W., Yuan, Z.Y., Jin, J.Q., Chen, H.M., Liu, H.Q., Nguyen, T.Q., Nguyen, S.N., Duong, T.V., Eto, K., Nishikawa, K., Matsui, M., Orlov, N.L., Stuart, B.L., Brown, R.M., Rowley, J.J.L., Murphy, R.W., Wang, Y.Y. & Che, J. (2018) Large-scale phylogenetic analyses provide insights into unrecognized diversity and historical biogeography of Asian leaf-litter frogs, genus Leptolalax (Anura: Megophryidae). Molecular Phylogenetics and Evolution, 124, 162–171.

    https://doi.org/10.1016/j.ympev.2018.02.020

    Das, I., Tron, R.K.L., Rangad, D. & Hooroo, R.N.K. (2010) A new species of Leptolalax (Anura: Megophryidae) from the sacred groves of Mawphlang, Meghalaya, north-eastern India. Zootaxa, 2339, 44–56.

    https://doi.org/10.11646/zootaxa.2339.1.2

    Dehling, J.M. (2012a) Redescription of Leptolalax gracilis (Günther, 1872) from Borneo and taxonomic status of two populations of Leptolalax (Anura: Megophryidae) from Peninsular Malaysia. Zootaxa, 3328, 20–34.

    Dehling, J.M. (2012b) Eine neue art der gattung Leptolalax (Anura: Megophryidae) vom Gunung Benom, West Malaysia, A new species of the genus Leptolalax (Anura: Megophryidae) from Gunung Benom, Peninsular Malaysia. Sauria, 34, 9–21.

    Dehling, J.M. & Matsui, M. (2013) A new species of Leptolalax (Anura: Megophryidae) from Gunung Mulu National Park, Sarawak, East Malaysia (Borneo). Zootaxa, 3670, 33–44.
    http://dx.doi.org/10.11646/zootaxa.3670.1.2

    Dring, J.C.M. (1983) Frogs of the genus Leptobrachella (Pelobatidae). Amphibia-Reptilia, 4, 89–102.

    https://doi.org/10.1163/156853883X00012

    Dubois, A. (1983) Note preliminaire sur le genre Leptolalax Dubois, 1980 (Amphibiens, Anoures), avec diagnose d’une espece novelle du Vietnam. Alytes, 2, 147–153.

    Dubois, A. (1987 “1986”) Miscellanea taxinomica batrachologia (I). Alytes, 5, 7–95.

    Duong, T.V., Do, D.T., Ngo, C.D., Nguyen, T.Q. & Poyarkov, N.A. Jr. (2018) A new species of the genus Leptolalax (Anura: Megophryidae) from southern Vietnam. Zoological Research/Dōngwùxué yánjiū, 39, 181–196.

    Eto, K., Matsui, M., Hamidy, A., Munir, M. & Iskandar, D.T. (2018) Two new species of the genus Leptobrachella (Amphibia: Anura: Megophryidae) from Kalimantan, Indonesia. Current Herpetology, 37, 95–105.

    https://doi.org/10.5358/hsj.37.95

    Eto, K., Matsui, M. & Nishikawa, K. (2015) Description of a new species of the genus Leptobrachella (Amphibia, Anura, Megophryidae) from Borneo. Current Herpetology, 34, 128–139.

    https://doi.org/10.5358/hsj.34.128

    Eto, K., Matsui, M. & Nishikawa, K. (2016) A new highland species of dwarf litter frog genus Leptobrachella (Amphibia: Anura: Megophryidae) from Sarawak. Raffles Bulletin of Zoology, 64, 194–203.

    Fei, L., Hu, S., Ye, C. & Huang, Y. (2009) Fauna Sinica. Amphibia. Vol. 2. Anura. Science Press, Beijing, 957 pp.

    Fei, L., Ye, C. & Huang, Y. (1991 “1990”) Key to Chinese Amphibia. Chongqing Branch Science and Technology Literature Press, Chongquing, 364 pp.

    Fei, L., Ye, C. & Jiang, J. (2010) Colored Atlas of Chinese Amphibians. Sichuan Publishing Group. Sichuan Publishing House of Science & Technology, Sichuan, 519 pp.

    Frost, D.R. (2018) Amphibian Species of the World: an Online Reference. Version 6.0. American Museum of Natural History, New York, USA. Available from: http://research.amnh.org/herpetology/amphibia/index.html (accessed 24 October 2018)

    Grismer, L.L., Grismer, J.L. & Youmans, T.M. (2004) A new species of Leptolalax (Anura: Megophryidae) from Pulau Tioman, West Malaysia. Asiatic Herpetological Research, 10, 8–10.

    Günther, A. (1872) On the reptiles and amphibians of Borneo. Proceedings of the scientific meetings of the Zoological Society of London, 1872, 586–600.

    Günther, A.C.L.G. (1895) The reptiles and batrachians of the Natuna Islands. Novitates Zoologicae, 2, 499–502.

    Hou, Y., Zhang, M., Hu, F., Li, S., Shi, S., Chen, J., Mo, X.Y. & Wang, B. (2018) A new species of the genus Leptolalax (Anura, Megophryidae) from Hunan, China. Zootaxa, 4444 (3), 247–266.

    http://dx.doi.org/10.11646/zootaxa.4444.3.2

    Humtsoe, L.N., Bordoloi, S., Ohler, A. & Dubois, A. (2008) Rediscovery of a long known species, Ixalus lateralis Anderson, 1871. Zootaxa, 1921, 24–34.

    Inger, R.F., Lakim, M., Biun, A. & Yambun, P. (1997) A new species of Leptolalax (Anura: Megophryidae) from Borneo. Asiatic Herpetological Research, 7, 48–50.

    https://doi.org/10.5962/bhl.part.18855

    Inger, R.F., Orlov, N. & Darevsky, I. (1999) Frogs of Vietnam: a report on new collections. Fieldiana Zoology, 92, 1–46.

    Inger, R.F. & Stuebing, R.B. (1992 "1991") A new species of frog of the genus Leptobrachella Smith (Anura: Pelobatidae), with a key to the species from Borneo. Raffles Bulletin of Zoology, 39, 99–103.

    Inger, R.F., Stuebing, R.B. & Tan, F.L. (1995) New species and new records of anurans from Borneo. Raffles Bulletin of Zoology, 43, 115–132.

    Inger, R.F. & Stuebing, R.B. (2005) A Field Guide to the Frogs of Borneo. 2nd Edition. Natural History Publications, Kota Kinabalu, 201 pp.

    https://doi.org/10.2307/1447418

    Jiang, J., Xie, F., Fei, L., Ye, C. & Zheng, M. (2002) Mating calls of six forms of pelobatid in Wawu Mountain national forest park, Sichuan, China (Anura: Pelobatidae). Zoological Research, 23, 89–94.

    Jiang, K., Yan, F., Suwannapoom, C., Chomdej, S. & Che, J. (2013) A new species of the genus Leptolalax (Anura: Megophryidae) from northern Thailand. Asian Herpetological Research, 4, 100–108.

    https://doi.org/10.3724/SP.J.1245.2013.00100

    Köhler, J., Jansen, M., Rodríguez, A., Kok, P.J.R., Toledo, L.F., Emmrich, M., Glaw, F., Haddad, C.F.B., Rödel, M.O. & Vences, M. (2017) The use of bioacoustics in anuran taxonomy: theory, terminology, methods and recommendations for best practice. Zootaxa, 4251 (1), 1–124.

    https://doi.org/10.11646/zootaxa.4251.1.1

    Lathrop, A., Murphy, R.W., Orlov, N. & Ho, C.T. (1998) Two new species of Leptolalax (Anura: Megophryidae) from northern Vietnam. Amphibia-Reptilia, 19, 253–267.

    https://doi.org/10.1163/156853898X00160

    Liu, C. (1950) Amphibians of western China. Fieldiana: Zoology Memoirs, 2, 1–400.

    https://doi.org/10.5962/bhl.part.4737

    Malkmus, R. (1992) Leptolalax pictus sp. n. (Anura: Pelobatidae) vom Mount Kinabalu/Nord-Borneo. Sauria, 14, 3–6.

    Malkmus, R., Manthey, U., Vogel, G., Hoffman, P. & Kosuch, J. (2002) Amphibians & Reptiles of Mount Kinabalu (North Borneo). ARG Ganter Verlag Kommanditgesellschaft, Ruggell, 424 pp.

    Mathew, R. & Sen, N. (2010 "2009") Description of a new species of Leptobrachium Tschudi, 1838 (Amphibia: Anura: Megophryidae) from Meghalaya, India. Records of the Zoological Survey of India, 109, 91–108.

    Matsui, M. (1997) Call characteristics of Malaysian Leptolalax with a description of two new species (Anura: Pelobatidae). Copeia, 1997, 158–165.

    https://doi.org/10.2307/1447851

    Matsui, M. (2006) Three new species of Leptolalax from Thailand (Amphibia, Anura, Megophryidae). Zoological Science, 23, 821–830.

    https://doi.org/10.2108/zsj.23.821

    Matsui, M., Belabut, D.M., Ahmad, N. & Yong, H. (2009) A new species of Leptolalax (Amphibia, Anura, Megophryidae) from Peninsular Malaysia. Zoological Science, 26, 243–247.

    https://doi.org/10.2108/zsj.26.243

    Matsui, M., Nishikawa, K. & Yambun, P. (2014a) A new Leptolalax from the mountains of Sabah, Borneo (Amphibia, Anura, Megophryidae). Zootaxa, 3753 (5), 440–452.

    https://doi.org/10.11646/Zootaxa.3753.5.3

    Matsui, M., Zainudin, R. & Nishikawa, K. (2014b) A new species of Leptolalax from Sarawak, western Borneo (Anura: Megophryidae). Zoological Science, 31, 773–779.

    https://doi.org/10.2108/zs140137

    Miller, M., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. Gateway Computing Environments Workshop, 2010, 1–8.

    https://doi.org/10.1109/gce.2010.5676129

    Nguyen, L.T., Poyarkov, N.A.Jr., Le, D.T., Vo, B.D., Phan H.T, Duong, T.V., Murphy, R.W. & Nguyen, S.N. (2018) A new species of Leptolalax (Anura: Megophryidae) from Son Tra Peninsula, central Vietnam. Zootaxa, 4388 (1), 1–21.

    http://doi.org/10.11646/zootaxa.4388.1.1

    Ohler, A., Marquis, O., Swan, S. & Grosjean, S. (2000) Amphibian biodiversity of Hoang Lien Nature Reserve (Lao Cai Province, northern Vietnam) with description of two new species. Herpetozoa, 13, 71–87.

    Ohler, A., Wollenberg, K.C., Grosjean, S., Hendrix, R., Vences, M., Ziegler, T. & Dubois, A. (2011) Sorting out Lalos: description of new species and additional taxonomic data on megophryid frogs from northern Indochina (genus Leptolalax, Megophryidae, Anura). Zootaxa, 3147, 1–83.

    Palumbi, S.R., Martin, A., Romano, S., McMillan, W.O., Stice, L. & Grabowski, G. (1991) The simple fool's guide to PCR. Department of Zoology, University of Hawaii, Honolulu, 47 pp.

    Poyarkov, N.A.Jr., Rowley, J.J.L., Gogoleva, S.I., Vassilieva, A.B., Galoyan, E.A. & Orlov, N.L. (2015) A new species of Leptolalax (Anura: Megophryidae) from the western Langbian Plateau, southern Vietnam. Zootaxa, 3931 (2), 221–252. https://doi.org/10.11646/Zootaxa.3931.2.3

    Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61, 539–542.

    https://doi.org/10.1093/sysbio/sys029

    Rowley, J.J.L. & Cao, T.T. (2009) A new species of Leptolalax (Anura: Megophryidae) from central Vietnam. Zootaxa, 2198, 51–60.

    Rowley, J.J.L., Dau, V.Q. & Cao, T.T. (2017b) A new species of Leptolalax (Anura: Megophryidae) from Vietnam. Zootaxa, 4273 (1), 61–79.

    http://dx.doi.org/10.11646/zootaxa.4273.1.5

    Rowley, J.J.L., Dau, V.Q., Hoang, H.D., Le, D.T.T., Cutajar, T.P. & Nguyen, T.T. (2017a) A new species of Leptolalax (Anura: Megophryidae) from northern Vietnam. Zootaxa, 4243 (3), 544–564.

    https://doi.org/10.11646/Zootaxa.4243.3.7

    Rowley, J.J.L., Dau, V.Q. & Nguyen, T.T. (2013) A new species of Leptolalax (Anura: Megophryidae) from the highest mountain in Indochina. Zootaxa, 3737 (4), 415–428.

    https://doi.org/10.11646/Zootaxa.3737.4.5

    Rowley, J.J.L., Hoang, D.H., Dau, V.Q. & Le, T.T.D. (2012) A new species of Leptolalax (Anura: Megophryidae) from central Vietnam. Zootaxa, 3321, 56–68.

    Rowley, J.J.L., Hoang, D.H., Le, T.T.D., Dau, V.Q. & Cao, T.T. (2010b) A new species of Leptolalax (Anura: Megophryidae) from Vietnam and further information on Leptolalax tuberosus. Zootaxa, 2660, 33–45.

    Rowley, J.J.L., Le, D.T.T., Tran, D.T.A. & Hoang, H.D. (2011) A new species of Leptolalax (Anura: Megophryidae) from southern Vietnam. Zootaxa, 2796, 15–28.

    Rowley, J.J.L., Stuart, B.L., Neang, T. & Emmett, D.A. (2010c) A new species of Leptolalax (Anura: Megophryidae) from northeastern Cambodia. Zootaxa, 2567, 57–68.

    Rowley, J.J.L., Stuart, B.L., Neang, T., Hoang, H.D., Dau, V.Q., Nguyen, T.T. & Emmett, D.A. (2015) A new species of Leptolalax (Anura: Megophryidae) from Vietnam and Cambodia. Zootaxa, 4039 (3), 401–417.

    https://doi.org/10.11646/Zootaxa.4039.3.1

    Rowley, J.J.L., Stuart, B.L., Richards, S.J., Phimmachak, S. & Sivongxay, N. (2010a) A new species of Leptolalax (Anura: Megophryidae) from Laos. Zootaxa, 2681, 35–46

    Rowley, J.J.L., Tran, D.T.A., Le, D.T.T., Dau, V.Q., Peloso, P.L.V., Nguyen, T.Q., Hoang, H.D., Nguyen, T.T. & Ziegler, T. (2016) Five new, microendemic Asian leaf-litter frogs (Leptolalax) from the southern Annamite Mountains, Vietnam. Zootaxa, 4085 (1), 63–102.

    https://doi.org/10.11646/Zootaxa.4085.1.3

    Sengupta, S., Sailo, S., Lalremsanga, H.T., Das, A. & Das, I. (2010) A new species of Leptolalax (Anura: Megophryidae) from Mizoram, North-eastern India. Zootaxa, 2406, 57–68.

    Smith, M.A. (1925) Contributions to the herpetology of Borneo. Sarawak Museum Journal, 3, 15–34.

    Smith, M.A. (1931) The herpetology of Mt. Kinabalu, North Borneo, 13,455 ft. Bulletin of the Raffles Museum, 5, 3–32.

    Sukumaran, J., Das, I. & Haas, A. (2010) Descriptions of the advertisement calls of some Bornean frogs. Russian Journal of Herpetology, 17, 189–194.

    Sung, Y., Yang, J. & Wang, Y. (2014) A new species of Leptolalax (Anura: Megophryidae) from southern China. Asian Herpetological Research, 5, 80–90.
    https://doi.org/10.3724/SP.J.1245.2014.00080

    Taylor, E.H. (1962) The amphibian fauna of Thailand. University of Kansas Science Bulletin, 43, 265–599.

    https://doi.org/10.5962/bhl.part.13347

    Vences, M., Thomas, M., Van der Meijden, A., Chiari, Y. & Vieites, D.R. (2005) Comparative performance of the 16S rRNA gene in DNA barcoding of amphibians. Frontiers in Zoology, 2, 1.

    https://doi.org/10.1186/1742-9994-2-5

    Wang, J., Yang, J.H., Li, Y., Lyu, Z.T., Zeng, Z.C., Liu Z.Y., Ye, Y.H. & Wang, Y.Y. (2018) Morphology and molecular genetics reveal two new Leptobrachella species in southern China (Anura, Megophryidae). ZooKeys, 776, 105–137.

    https://doi.org/10.3897/zookeys.776.22925

    Xu, J., Xie, F., Jiang, J., Mo, Y. & Zheng, Z. (2005) The acoustic features of the mating call of 12 anuran species. Chinese Journal of Zoology, 40, 12–19.

    Yang, J., Wang, Y., Chen, G. & Rao, D. (2016) A new species of the genus Leptolalax (Anura: Mehophryidae) from Mt. Gaoligongshan of Western Yunnan Province, China. Zootaxa, 4088 (3), 379–394.

    https://doi.org/10.11646/Zootaxa.4088.3.4

    Yang, J., Zeng, Z. & Wang, Y. (2018) Description of two new sympatric species of the genus Leptolalax (Anura: Megophryidae) from western Yunnan of China. PeerJ, 6, e4586.

    https://doi.org/10.7717/peerj.4586

    Yuan, Z., Sun, R., Chen, J., Rowley, J.J.L., Wu, Z., Hou, S., Wang, S. & Che, J. (2017) A new species of the genus Leptolalax (Anura: Megophryidae) from Guangxi, China. Zootaxa, 4300 (4), 551–570.

    https://doi.org/10.11646/zootaxa.4300.4.5