Abstract
Salamandrina is a monotypic genus of the family Salamandridae endemic to Italy. Forty five individuals of the spectacled salamander, Salamandrina terdigitata, representing 11 populations throughout the whole distribution range were examined for sequence variation of three mitochondrial DNA genes encoding the 12S and 16S ribosomal RNA’s and cytochrome b (1324 bp). The results indicate the existence of two genetically distinct and geographically non-overlapping mtDNA lineages. The first lineage includes the southern populations and the second one comprises the central-northern populations. The degree of genetic divergence between the two groups is high and comparable to distances calculated from homologous sequences available in GenBank between other Salamandrid species. As a result, the genus Salamandrina probably requires splitting into two species. We also compare substitution rates associated with the mitochondrial genes employed across all Salamandrids studied so far, and discuss two possible palaeogeographic scenarios which could have shaped the splitting of the two Salamandrina lineages. These findings have also important implications for management and conservations of the spectacled salamander, which is protected under several international and regional conventions and directives.References
Andreone, F. & Luiselli, L. (2000) The Italian batrachofauna and its conservation status: a statistical assessment. Biological Conservation, 96, 197–208.
Alvarez,. W. (1974) Sardinia and Corsica, One Microplate or Two? Rendiconti del Seminario della Facoltà di Scienze dell’Università di Cagliari. Libreria Cocco, Cagliari.
Angelini, C., Antonelli, D. & Utzeri, C. (2001) Aspetti di fenologia riproduttiva di Salamandrina terdigitata (Lacépède, 1788) in Italia centrale. Pianura, 13, 105–108.
Angelini, C. & Cari, B. (2002) Gli anfibi dei Colli Albani, Latium. In: 4° Congresso Nazionale Societas Herpetologica Italica, Ercolano (Napoli), 18-22 giugno 2002. Abstract, p. 55.
Arntzen, J.W., Smithson, A. & Oldham, R.S. (1999) Marking and tissue sampling effects on body condition and survival in the newt Triturus cristatus. Journal of Herpetology, 33, 567–576.
Barbieri, F. & Pellegrini, M. (in press). Salamandrina terdigitata. In: Atlante degli Anfibi e dei Rettili d'Italia - Atlas of Italian Amphibians and Reptiles, Edizioni Polistampa, Firenze.
Barbieri, F., Zuffi, M.A.L. & Tiso, E. (1989) Salamandrina terdigitata nel versante padano dell'Appennino settentrionale. Congresso Nazionale Unione Zoologica Italiana, Camerino, 12-16.09.1988. Bollettino di Zoologia, 55 (suppl.), 1988, 41, Abstract, 53.
Barbieri, F. (2001) La salamandrina dagli occhiali (Salamandrina terdigitata) nel versante padano dell’Appennino centro-settentrionale (Amphibia: Salamandridae). Pianura, 13, 101–104.
Boccaletti, M., Ciaranfi, N., Casentino, D., Deiana, G., Gelati, R., Lentini, F., Massari, F., Moratti, G., Pescatore, T., Ricci Lucchi, F. & Tortorici L., (1990) Palinspatic restoration and palaeogeographic reconstruction of the peri-Tyrrhenian area during the Neogene. Palaeogeography, Palaeoclimatology, Palaeoecology, 77, 41–50.
Bonin, B., Chotin, P., Giret, A. & Orsini, J. B. (1979) Etude du bloc corsosarde sur documents satellites: le problème des mouvements differentiels entre les deux iles. Revue de Geographie Physique et de Geologie Dynamique, 21, 147–154.
Caccone, A., Milinkovitch, M.C., Sbordoni, V. & Powell, J.R. (1994) Molecular biogeography: using the Corsica-Sardinia microplate disjunction to calibrate mitochondrial rDNA evolutionary rates in mountain newts (Euproctus). Journal of Evolutionary Biology, 7, 227–245.
Caccone, A., Milinkovitch, M.C., Sbordoni, V. & Powell, J.R. (1997) Mitochondrial DNA rates and biogeography in European newts (genus Euproctus). Systematic Biology, 46 (1), 126–144.
Carranza, S. & Arnold, E.N. (2004). History of West Mediterranean newts, Pleurodeles (Amphibia: Salamandridae), inferred from old and recent DNA sequences. Systematics and Biodiversity, 1 (3), 327–337.
Clement, M., Posada, D. & Crandall, K.A. (2000) TCS: a computer program to estimate gene genealogies. Molecular Ecology, 9, 1657–1669.
Corsetti, L. (1999) Habitat characteristics of the spectacled salamander Salamandrina terdigitata (Lacépède, 1788) in southern Latium (Central Italy). Amphibia-Reptilia, 20, 77–82.
Doyle, J.J., & Doyle, J.L. (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemistry Bulletin, 19, 11–15.
Duermeijer, C.E., van Vugt, N., Langeris, C.G., Meulenkamp, J.E. & Zachariasse, N.J. (1998) A major late Tortonian rotation phase in the opening of the Tyrrhenian basin. Tectonophysics, 287, 233–249.
Estes, R. (1981) Gymnophiona, Caudata. In: Handbuch der Paläoherpetologie (Encyclopedia of Paleoherpetology), 2 (2), Gustav Fischer Verlag, Stuttgart, pp. 1–115.
Farris, J.S. (1970) Methods for computing Wagner trees. Systematic Zoology, 18, 374–385.
Farris, J.S., Kallersjo, M., Kluge, A.G., & Bult, C. (1994) Testing significance of congruence. Cladistics, 10, 315–320.
Felsenstein, J. (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. Journal of Molecular Evolution, 17, 368–376.
Felsenstein, J. (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 39, 783–791.
Fromhage, L., Vences, M. & Veith M. (2004) Testing alternative vicariance scenarios in Western Mediterranean discoglossid frogs. Molecular Phylogenetics and Evolution, 31, 308–322.
Goldman, N. (1993) Statistical tests of models of DNA substitution. Journal of Molecular Evolution, 36, 182–198.
Huelsenbeck, J.P. (2000) MrBAYES: Bayesian inference of phylogeny. Distributed by the author. New York: Department of Biology, Univ. of Rochester.
Kocher, T.D., Thomas, W.K., Meyer, A., Edwards, S.V., Paabo, S., Villablanca, F.X. & Wilson, A.C. (1989) Dynamics of mitochondrial DNA evolution in mammals: amplification and sequencing with conserved primers. Proceedings of National Academy of Sciences, 86, 6196–6200.
La Greca, M. (1984) L’origine della fauna italiana. Le Scienze, 187, 66–79.
Lacépède, B.G.E. de La Ville, Comte de, (1788) Histoire naturelle de quadrupèdes ovipares, et des serpentes. Vol. I. Paris: Imprimerie du Roi (Hotel de Thou).
Larget, B. & Simon, D. (1999) Markov chain Monte Carlo algorithm for Bayesian analysis of phylogenetic trees. Molecular Biology and Evolution, 16, 750–759.
Macgregor, H.C., Sessions, S.K. & Amtzen, J.W. (1990) An integrative analysis of phylogenetic relationships among newts of the genus Triturus (family Salamandridae), using comparative biochemistry, cytogenetics and reproductive interactions. Journal of Evolutionary Biology, 3, 329–373.
Mau, B. & Newton, M. (1997) Phylogenetic inference for binary data on dendrograms using Markov chain Monte Carlo. Journal of Computational and Graphical Statistics, 6, 122–131.
Mau, B., Newton, M. & Larget, B. (1999). Bayesian phylogenetic inference via Markov chain Monte Carlo methods. Biometrics, 55, 1–12.
Orszag-Sperber, F., Butterlin, J., Clermonte, J., Colchen, M., Guiraud, R., Poisson, A. & Ricou, L.E. (1993) Tortonian Palaeoenvironments (11.5-6 Ma) and map. In: Dercourt, J., Ricou, L.E., Vrielynck, B. (Eds.), Atlas Tethys, Paleoenvironmental Maps. Gauthier-Villars, Paris, pp. 243–258.
Pinna, G. (1989) Il periodo Neogenico. In: Pinna, G., Il grande libro dei fossili, Biblioteca Universale Rizzoli, pp. 253–257.
Posada, D. & Crandall, K.A. (1998) MODELTEST: testing the model of DNA substitution. Bioinformatics, 14, 817–818.
Rannala, B., & Yang, Z. (1996) Probability distribution of molecular evolutionary trees: a new method of phylogenetic inference. Journal of Molecular Evolution, 43, 304–311.
Rowe, G., Beebee, T.J.C. & Burke T. (1999) Microsatellite heterozygosity, fitness and demography in natter jack toads, Bufo calamita, metapopulations. Oikos, 88, 85–92.
Saitou, N. & Nei, M. (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4, 406–425.
Sanchiz, B. (1977) Nuevos anfibios del Neogeno y Cuaternario de Europa. Origen, desarrollo y relaciones de la batracofauna espanola. Universidad Complutense, Madrid, tesis doctoral, 3 vols. 863 pp.
Sanchiz, B. (1983) The fossil record of living European Amphibians. Second Ordinary General Meeting of the Societas Europea Herpetologica. León 12-16 September 1983, Abstracts,16–17.
Sanchiz, B. & Mlynarski, M. (1979) Pliocene salamandrids (Amphibia, Caudata) from Poland. Acta zoologica cracoviense, 24, 175–188.
Savi, P. (1821) Descrizione (inedita) di una nuova specie di Salamandra terrestre, Salamandra perspicillata, Nob. del dottore Paolo Savi, ajuto del professore di botanica dell'Università di Pisa. In: Biblioteca italiana, (Giornale di Lettura, Scienze ed Arti), Milano, 22 (anno VI), 2, pp. 228–230.
Scalera, R. (1998) Salamandre. In: Bulgarini, F., Calvari, E., Fraticelli, F., Petretti, F., Sarrocco, S. (Eds.) Libro Rosso degli Animali d’Italia - Vertebrati. WWF Italia, Roma, 40 pp.
Shimodaira, H. & Hasegawa, M. (1999) Multiple comparisons of log-likelihoods with applications to phylogenetic inference. Molecular Biology and Evolution, 16, 1114–1116.
Simon, C., Frati, F., Beckenbach, A., Crespi, B., Liu, H. & Flook, P. (1994) Evolution, weighting and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Annals of the Entomological Society of America, 87, 651–701.
Spolsky, C., Phillips, C.A. & Uzzell, T. (1992) Antiquity of clonal salamander lineages revealed by mtDNA. Nature, 356, 706–708.
Steinfartz, S., Hwang, U.W., Tautz, D., Öz, M. & Veith, M. (2002). Molecular phylogeny of the salamandrid genus Neurergus: evidence for an intrageneric switch of reproductive biology. Amphibia-Reptilia, 23, 419–431.
Swofford, D.L. (2002) PAUP*: Phylogenetic Analysis Using Parsimony (*and other methods). Version 4.0β10. Sinauer Associates, Sunderland, MA
Taberlet, P. & Luikart, G. (1999) Non-invasive genetic sampling and individuals identification. Biological Journal of the Linnean Society, 68, 41–45.
Tamura, K. & Nei, M. (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzee. Molecular Biology and Evolution. 10, 512–526.
Tan, A.M. & Wake, D.B. (1995) MtDNA phylogeography of the California newt, Taricha torosa (Caudata, Salamandridae). Molecular Phylogenetics and Evolution, 4, 383–394.
Templeton, A. (1983) Phylogenetic inference from restriction endonuclease cleavage site maps with particular reference to the evolution of humans and the apes. Evolution, 37, 221–244.
Templeton, A.R., Crandall, K.A. & Sing, C.F. (1992) A cladistic analysis of phenotypic associations with haplotypes inferred from restriction endonuclease mapping and DNA sequence data. III. Cladogram estimation. Genetics, 132, 619–633.
Titus, T.A. & Larson, A. (1995) A molecular phylogenetic perspective on the evolutionary radiation of the salamander family Salamandridae. Systemathic Biology, 44(2), 125–151.
Thomson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. & Higgins, D.G. (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25, 4876–4882
Thorn, R. (1969) Les Salamandres d'Europe, d'Asie et d'Afrique du Nord. Description et moeurs de toutes les espèces et sous-espèces d'Urodèles de la Région Paléartique d' après l'état de 1967. Lechevalier, Paris 1968, 376 pp., 56 figures, 16 plates, 11 charts.
Vanni, S. (1980) Note sulla Salamandrina dagli occhiali (Salamandrina terdigitata (Lacépède, 1788)) in Toscana (Amphibia: Salamandridae). Atti della Società Toscana di Scienze Naturali, Mem., ser.B, 87, 135–159.
Veith, M., Mayer, C., Samraoui, B., Barroso, D.D. & Bogaerts, S. (2004) From Europe to Africa and vice versa: evidence for multiple intercontinental dispersal in ribbed salamanders (Genus Pleurodeles). Journal of Biogeography, 31, 159–171.
Zuffi, M.A.L. (1999) Salamandrina terdigitata (Lacépède, 1788) — Brillensalamander. In: Grossenbacher, K. & Thiesmeir (eds.). Handbuch der Reptilien und Amphibien Europas. Band 4/1 Schwanzlurche (Urodela) I, Aula-Verlag, Wiebelsheim, pp. 229–246.