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A new Pardosa species from northern Iran (Araneae, Lycosidae)



A study of Pardosa pontica (Thorell, 1875) in Iran indicates two distinct morphospecies. A detailed comparison of these morphospecies and types of P. pontica reveals that populations from Mazandaran, Golestan and North Khorasan Provinces of Iran and Balkan Welaýaty of Turkmenistan belong to a newly described species, P. mirzakhaniae sp. n., and populations in western and easternmost Iran belong to P. pontica. Although the two morphospecies differ in colour, size and spination, no molecular differences in 16S rRNA and COI were detected.



Araneae, wolf spiders, Aranei, Pardosini, monticola-group, Middle East, Mazandaran, redescription

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Astrin, J.J., Höfer, H., Spelda, J., Holstein, J., Bayer, S., Hendrich, L., Huber, B.A., Kielhorn, K.H., Krammer, H.J., Lemke, M., Monje, J.C., Morinière, J., Rulik, B., Petersen, M., Janssen, H. & Muster, C. (2016) Towards a DNA Barcode Reference Database for Spiders and Harvestmen of Germany. PLoS ONE, 11 (9), e0162624.

Ballarin, F., Marusik, Y.M., Omelko, M.M. & Koponen, S. (2012) On the Pardosa monticola species-group (Araneae: Lycosidae) from Middle Asia. Arthropoda Selecta, 21 (2), 161–182.

Blackledge, T.A., Coddington, J.A. & Agnarsson, I. (2009a) Fecundity increase supports adaptive radiation hypothesis in spider web evolution. Communicative & Integrative Biology, 2, 459–463.

Brewer, M.S., Carter, R.A., Croucher, P.J.P. & Gillespie, R.G. (2015) Shifting habitats, morphology, and selective pressures: developmental polyphenism in an adaptive radiation of Hawaiian spiders. Evolution, 69, 162–178.

Casiraghi, M., Labra, M., Ferri, E., Galimberti, A. & De Mattia, F. (2010) DNA barcoding: a six-question tour to improve users’ awareness about the method. Briefings in Bioinformatics, 11, 440‒453.

Chang, J., Song, D. & Zhou, K. (2007) Incongruous nuclear and mitochondrial phylogeographic patterns in two sympatric lineages of the wolf spider Pardosa astrigera (Araneae: Lycosidae) from China. Molecular Phylogenetics and Evolution, 42, 104–121.

Dai, L., Zheng, X., Kong, L. & Li, Q. (2012) DNA barcoding analysis of Coleoidea (Mollusca: Cephalopoda) from Chinese waters. Molecular Ecology Resources, 12, 437‒447.

Dasmahapatra, K.K., Elias, M., Hill, R.I., Hoffman, J.I. & Mallet, J. (2010) Mitochondrial DNA barcoding detects some species that are real, and some that are not. Molecular Ecology Resources, 10, 264–273.–0998.2009.02763.x

Dondale, C.D. & Redner, J.H. (1990) The wolf spiders, nursery web spiders, and lynx spiders of Canada and Alaska, Araneae: Lycosidae, Pisauridae, and Oxyopidae. Agriculture Canada, Ottawa, 383 pp.

Drensky, P. (1936) Katalog der echten Spinnen (Araneae) der Balkanhalbinsel. Opis na Paiatzite ot Balkanikia polouostrow. Spisanié na Beulgarskata Akademia na Naoukite, 32, 1–223.

Drensky, P. (1942) Die Spinnenfauna Bulgariens V. Mitteilungen aus den Königlichen Naturwissenschaftlichen Instituten in Sofia, 15, 33–60.

Fabricius, J.C. (1807) Die neueste Gattungs-Eintheilung der Schmetterlinge aus den Linnéischen Gattungen Papilio und Sphinx. Magazin für Insektenkunde, 6, 277–295.

Fuhn, I.E. & Niculescu-Burlacu, F. (1971) Fauna Republicii Socialiste România. Vol. V. Fascicula 3. Arachnida: Familia Lycosidae. Academia Republicii Socialiste România, Bucuresti, 253 pp.

Funk, D.J. & Omland, K.E. (2003) Species-level paraphyly and polyphyly: frequency, causes, and consequences, with insights from animal mitochondrial DNA. Annual Review of Ecology, Evolution, and Systematics, 34, 397‒423.

Gene Codes Corporation (2011) Sequencher® version 5.0 DNA sequence analysis software, Ann Arbor, Michigan, USA. Available from: (accessed 23 January 2018)

Gillespie, R.G., Howarth, F.G. & Roderick, G.K. (2001) Adaptive radiation. In: Levin, S.A. (Ed.), Encyclopedia of Biodiversity. Vol. 1. Academic Press, San Diego, pp. 25–44.

Glor, R.E. (2010) Phylogenetic insights on adaptive radiation. The Annual Review of Ecology, Evolution, and Systematics, 41, 251–270.

Hough, G.De.N. (1899) Some North American genera of the dipterous group, Calliphorinae Girschner. Entomological News, 10, 62–66.

Kimura, M. (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16, 111‒120.

Koch, C.L. (1847) Die Arachniden. Vierzehnter Band, Funfzehnter Band & Sechszehnter Band. In der C. H. Zeh'schen Buchhandlung, Nürnberg, 122 pp. 136 pp. & 80 pp. [pp. 89–210, pp. 1–136 & pp. 1–80]

Kronestedt, T. & Marusik, Y.M. (2011) Studies on species of Holarctic Pardosa groups (Araneae, Lycosidae). VII. The Pardosa tesquorum-group. Zootaxa, 3131 (1), 1–34.

Lasut, L. (2015) Testing the suitability of DNA barcoding in spiders (Araneae). PhD Thesis. Universität Bern, Bern, 141 pp.

Marusik, Y.M. & Fritzén, N.R. (2009) A new wolf spider species in the Pardosa monticola-group (Araneae: Lycosidae) from Xinjiang, China. Journal of Natural History, 43, 411–422.

Marusik, Y.M. & Ballarin, F. (2011) Redescription of the Himalaian Pardosa flavisterna Caporiacco, 1935 (Aranei: Lycosidae) with notes of the Pardosa nebulosa species-group. Proceedings of the Zoological Institute of the Russian Academy of Sciences, 315 (1), 63–69.

Marusik, Y.M., Ballarin, F. & Omelko, M.M. (2012) On the Pardosa monticola-species group from Iran (Araneae: Lycosidae). Zoology in the Middle East, 56, 111–123.

Mcheidze, T.S. (1946) New species of spiders in Georgia [Nov'e vid' paukov v Gruzii]. Bulletin du Museum de Georgie, 13 (A), 285–302. [in Georgian and Russian]

Mcheidze, T.S. (1997) Spiders of Georgia: Systematics, Ecology, Zoogeographic Review. Tbilisi University Publishing House, Tbilisi, 390 pp. [in Georgian]

Mutanen, M., Hausmann, A., Hebert, P.D.N., Landry, J.-F., deWaard, J.R. & Huemer, P. (2012) Allopatry as a Gordian knot for taxonomists: patterns of DNA barcode divergence in Arctic-Alpine Lepidoptera. PLoS ONE, 7, e47214.

Nadolny, A.A., Omelko, M.M., Marusik, Y.M. & Blagoev, G. (2016) A new species of spider belonging to the Pardosa lugubris-group (Araneae: Lycosidae) from Far East Asia. Zootaxa, 4072 (2), 263–281.

Neige, P. (2015) The Phenomenon of Evolutionary Radiation. In: Neige, P. (Ed.), Events of Increased Biodiversity. ISTE Press, Elsevier, Oxford, pp. 47–64.

Nussey, D.H., Postma, E., Gienapp, P. & Visser, M.E. (2005) Selection on heritable phenotypic plasticity in a wild bird population. Science, 310, 304–306.

Pfennig, D.W., Wund, M.A., Snell-Rood, E.C., Cruickshank, T., Schlichting, C.D. & Moczek, A.P. (2010) Phenotypic plasticity’s impacts on diversification and speciation. Trends in Ecology and Evolution, 25, 459–467.

Polchaninova, N.Y. & Prokopenko, E.V. (2013) Catalogue of the spiders (Arachnida, Aranei) of Left–Bank Ukraine. Arthropoda Selecta, 1–268.

Rambaut, A. (1999) Se-Al v2.0a11: sequence alignment editor. Available from: (accessed 19 July 2017)

Roewer, C.F. (1955a) Die Araneen der Österreichischen Iran-Expedition 1949/50. Sitzungsberichte der Österreichischen Akademie der Wissenschaften, 164, 751–782.

Roewer, C.F. (1955b) Katalog der Araneae von 1758 bis 1940. Natura, Bremen, Bruxelles, 1751 pp.

Shafaie, S., Mirshamsi, O., Aliabadian, M., Moradmand, M. & Marusik, Y.M. (2017) A discovery of hidden species related to Pardosa pontica (Thorell, 1875) in northern Iran. 30th European Congress of Arachnology, Nottingham, 162 pp. [August 2017, 112]

Sim, K.A. (2013) Genetic analysis of Pardosa wolf spiders (Araneae: Lycosidae) across the
northern Nearctic
. Msc Thesis. McGill University, Montreal, 98 pp.

Sim, K.A., Buddle, C.M. & Wheeler, T.A. (2014) Species boundaries of Pardosa concinna and P. lapponica (Araneae: Lycosidae) in the northern Nearctic: morphology and DNA barcodes. Zootaxa, 3884 (2), 169–178.

Sundevall, C.J. (1833) Conspectus Arachnidum. C.F. Berling, Londini Gothorum, 39 pp.

Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. & Kumar, S. (2011) MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution, 28, 2731–2739.

Thorell, T. (1856) Recensio critica aranearum suecicarum quas descripserunt Clerckius, Linnaeus, de Geerus. Nova Acta Regiae Societatis Scientiarum Upsaliensis, 2 (1), 61–176.

Thorell, T. (1875a) Verzeichniss südrussischer Spinnen. Horae Societatis Entomologicae Rossicae, 11, 39–122.

Thorell, T. (1875b) Descriptions of several European and North African spiders. Bihang till Kongliga Svenska Vetenskaps-Akademiens Handlingar, 13 (5), 1–203.

Tongiorgi, P. (1966) Wolf spiders of the Pardosa monticola-group (Araneae: Lycosidae). Bulletin of the Museum of Comparative Zoology at Harvard College, 134, 335–359.

Whitworth, T.L., Dawson, R.D., Magalon, H. & Baudry, E. (2007) DNA barcoding cannot reliably identify species of the blowfly genus Protocalliphora (Diptera: Calliphoridae). Proceedings of the Royal Society, Biological Science, 274, 1731–1739.

World Spider Catalog (2017) World Spider Catalog. Version 18.5. Natural History Museum of Bern. Available from: (accessed 20 July 2017)

Zamani, A. (2015) The spider collection (Arachnida: Araneae) of the Zoological Museum of the Iranian Research Institute of Plant Protection, with new species records for Iran. Arachnologische Mitteilungen, 50, 11–18.

Zyuzin, A.A. (1979) A taxonomic study of Palaearctic spiders of the genus Pardosa (Aranei, Lycosidae). Part 1. The taxonomic structure of the genus. Entomologicheskoe Obozrenie, 58 (2), 431–447. [in Russian]

Zyuzin, A.A. & Logunov, D.V. (2000) New and little-known species of the Lycosidae from Azerbaijan, the Caucasus (Araneae, Lycosidae). Bulletin of the British Arachnological Society, 11, 305–319.



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