Open Access Open Access  Restricted Access Subscription or Fee Access

A new species of Hyloscirtus (Anura, Hylidae) from the Colombian and Venezuelan slopes of Sierra de Perijá, and the phylogenetic position of Hyloscirtus jahni (Rivero, 1961)



A new species of Hyloscirtus, belonging to the H. bogotensis species Group, is described from the Venezuelan and Colombian slopes of the Sierra de Perijá. The new species can be readily distinguished from its congeners by the combination of the following characters: mental gland present, disc-shaped and small; ulnar, outer, and inner tarsal folds present; calcar tubercle absent; whitish stripes on external border of upper eyelids and supratympanic folds, longitudinally on the mid-dorsum, on supracloacal fold, outer ulnar folds, inner and outer tarsal folds, and also on dorsal internal surface of shanks. We estimate phylogenetic relationships based on mtDNA (spanning fragments of 12S rRNA, tRNA-Val and 16S rRNA), of all Hyloscirtus species available in Genbank, as well as the new species described herein, H. callipeza, H. jahni, and H. platydactylus, all of which have not been previously sequenced. Our molecular data support the hypothesis of the new species as sister species of H. callipeza and indicates that H. jahni does not belong to the H. bogotensis species Group, but rather is sister species of all other Hyloscirtus (sensu Faivovich et al. 2005). Based on this last result we propose a new species group for H. jahni and the synonymy of Colomascirtus in Hyloscirtus. We also provide the first description of the advertisement call of H. callipeza. With the new species described herein, the number of Hyloscirtus species increases to 37.



Amphibia, Advertisement call, Amphibia, Andes, Colomascirtus, Hylinae, Hyloscirtus bogotensis species Group, Hyloscirtus callipeza, integrative taxonomy, phylogeny

Full Text:

PDF (15MB)


Acosta-Galvis, A.R. (2000) Ranas, salamandras y caecilias (Tetrapoda: Amphibia) de Colombia. Biota Colombiana, 1, 289–319.

Almendáriz, A., Brito, J., Batallas, D. & Ron, S. (2014) Una especie nueva de rana arbórea del género Hyloscirtus (Amphibia: Anura: Hylidae) de la Cordillera del Cóndor. Papéis Avulsos de Zoologia, 54, 33–49.

Barrio A., C.L. (2004) Amphibians of Venezuela. Systematic list, distribution and references, an update. Revista de Ecología Latinoamericana, 9, 1–48.

Barrio-Amorós, C.L., Rojas-Runjaic, F. & Barros, T.R. (2010) Two new Pristimantis (Anura: Terrarana: Strabomantidae) from the Sierra de Perijá, Venezuela. Zootaxa, 2329, 1–21.

Bioacoustics Research Program (2008) Raven Pro: Interactive Sound Analysis Software (Version 1.3) [Computer software]. The Cornell Lab of Ornithology, Ithaca, NY, Available from: (accessed 20 December 2017)

Boulenger, G.A. (1882) Catalogue of the Batrachia Salientia s. Ecaudata in the Collection of the British Museum. 2nd Edition. Taylor and Francis, London, viii + 127 pp.

Boulenger, G A. (1905) Descriptions of new tailless batrachians in the collection of the British Museum. Annals and Magazine of Natural History, Series 7, 16, 180–184.

Boulenger, G.A. (1908) Descriptions of new batrachians and reptiles discovered by Mr. M.G. Palmer in south-western Colombia. Annals and Magazine of Natural History, Series 8, 2, 515–522.

Brunetti, A.E., Hermida, G.N., Luna, M.C., Barsotti, A.M.G., Jared, C., Antoniazzi, M.M., Rivera-Correa, M., Berneck, B.V.M. & Faivovich, J. (2015) Diversity and evolution of sexually dimorphic mental and lateral glands in Cophomantini treefrogs (Anura: Hylidae: Hylinae). Biological Journal of the Linnean Society, 114, 12–34.

Coloma, L.A., Carvajal-Endara, S., Dueñas, J.F., Paredes-Recalde, A., Morales-Mite, M., Almeida-Reinoso, D., Tapia, E.E., Hutter, C.R., Toral, E. & Guayasamin, J.M. (2012) Molecular phylogenetics of stream treefrogs of the Hyloscirtus larinopygion group (Anura: Hylidae), and description of two new species from Ecuador. Zootaxa, 3364, 1–78.

De Villamañán, A. & De Santelos, P. (1957) Los “Japreria”, quienes son y donde viven II. Venezuela Misionera, 19, 101–2.

Duellman, W.E. (1970) The hylid frogs of Middle America. Vol. 1. Monograph of the Museum of Natural History, University of Kansas, No. 1, 1–427.

Duellman, W.E. (1972) A review of the Neotropical frogs of the Hyla bogotensis group. Occasional Papers of the Museum of Natural History. University of Kansas, 11, 1–31.

Duellman, W.E. (1989) New species of hylid frogs from the Andes of Columbia and Venezuela. Occasional Papers of the Museum of Natural History. University of Kansas, 131, 1–12. Available from: (Accessed 19 Feb. 2018)

Duellman, W.E. & Altig, R.I. (1978) New species of tree frogs (family Hylidae) from the Andes of Colombia and Ecuador. Herpetologica, 34, 177–185.

Duellman, W.E. & Hillis, D.M. (1990) Systematics of frogs of the Hyla larinopygion group. Occasional Papers of the Museum of Natural History. University of Kansas, 134, 1–23.

Duellman, W.E. Marion, A.B. & Hedges, S.B. (2016) Phylogenetics, classification, and biogeography of the treefrogs (Amphibia: Anura: Arboranae). Zootaxa, 4104 (1), 001–109.

Dunn, E.R. (1931) New frogs from Panama and Costa Rica. Occasional Papers of the Boston Society of Natural History, 5, 385–401.

Faivovich, J., Haddad, C.F.B., De Garcia, P.C., Frost, D.R., Campbell, J.A. & Wheeler, W.C. (2005) Systematic review of the frog family Hylidae, with special reference to Hylinae: a phylogenetic analysis and taxonomic revision. Bulletin of the American Museum of Natural History, 294, 1–240.[0001:SROTFF]2.0.CO;2

Faivovich, J., Moravec, J., Cisneros-Heredia, D.F. & Köhler, J. (2006) A new species of the Hypsiboas benitezi group from the western Amazon basin (Amphibia: Anura: Hylidae). Herpetologica, 62, 96–108.

Faivovich, J., McDiarmid, R.W. & Myers, C.W. (2013) Two new species of Myersiohyla (Anura: Hylidae) from Cerro de la Neblina, Venezuela, with comments on other species of the genus. American Museum Novitates, 3792, 1–63.

Guayasamin, J.M., Rivera-Correa, M., Arteaga, A., Culebras, J., Bustamante, L., Pyron, R.A., Peñafiel, N., Morochz, C. & Hutter, C.R. (2015) Molecular phylogeny of stream treefrogs (Hylidae: Hyloscirtus bogotensis Group), with a new species from the Andes of Ecuador. Neotropical Biodiversity, 1, 2–21.

Halbmayer, E. (2013) Contemporary Carib-speaking Amerindians. A bibliography of social anthropological and linguistic resources. Curupira Workshop, Marburg, 269 pp.

Holdridge, L.R. (1967) Life zone ecology. Tropical Science Center, San Jose, 206 pp.

Huber, O. & Alarcón, C. (1988) Mapa de vegetación de Venezuela. 1:2.000.000. Ministerio del Ambiente y de los Recursos Naturales Renovables, Caracas. [map]

IDEAM (2016) Instituto de Hidrología, Meteorología y Estudios Ambientales de Colombia, version 2016. Available from: (accessed 9 April 2016)

Jerdon, T.C. (1870) Notes on Indian herpetology. Proceedings of the Asiatic Society of Bengal, 1870, 66–85.

Kaplan, M. (1997) A new species of Colostethus from the Sierra Nevada de Santa Marta (Colombia) with comments on intergeneric relationships within the Dendrobatidae. Journal of Herpetology, 31, 369–375.

Kok, P.J.R. & Kalamandeen, M. (2008) Introduction to the taxonomy of the amphibians of Kaieteur National Park, Guyana. Abc Taxa, 5, 1–278.

Kumar, S., Stecher, G. & Tamura, K. (2016) MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33, 1870–1874.

La Marca, E. (1985) Systematics and ecological observations on the Neotropical frogs Hyla jahni and Hyla platydactyla. Journal of Herpetology, 19, 227–237.

La Marca, E. (1994) Geographic Distribution. Hyla platydactyla (NCN). Herpetological Review, 25, 160–161.

La Marca, E. & García-Pérez, J.E. (2004) Hyloscirtus jahni. The IUCN Red List of Threatened Species 2004: e.T55518A11323517.

Lanfear, R., Frandsen, P.B., Wright, A.M., Senfeld, T. & Calcott, B. (2016) PartitionFinder 2: new methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses. Molecular Biology and Evolution, 34, 772–773.

Larsson, A. (2014). AliView: a fast and lightweight alignment viewer and editor for large data sets. Bioinformatics, 30 (22), 3276–3278.

Lewis M., Simons, P.G.F. & Fennig, C.D. (Eds.) (2016) Ethnologue: Languages of the World. Nineteenth edition. Dallas, Texas: SIL International. Online version. Available from: (accessed 8 March 2016)

Masciangioli, P. & Febres, G. (2000) Climatología de la cuenca de Maracaibo. In: Rodríguez, G. (Ed.), El sistema de Maracaibo, Biología y Ambiente. 2nd Edition. Instituto Venezolano de Investigaciones Científicas, Caracas, pp. 21–32.

Melin, D.E. (1941) Contributions to the knowledge of the Amphibia of South America. Göteborgs Kungl. Vetenskaps-och Vitterhets-samhälles. Handlingar. Serien B, Matematiska och Naturvetenskapliga Skrifter, 1, 1–71.

Myers, C.W. & Duellman, W.E. (1982) A new species of Hyla from Cerro Colorado, and other tree frog records and geographical notes from western Panama. American Museum Novitates, 2752, 1–32. Available from: (Accessed 19 Feb. 2018)

Oquendo, L. (2004) Is Japreria a Yukpa dialect? Lingua Americana, 8, 87–98.

Peters, W.C.H. (1882) Den Namen der batrachiergattung Hylonomus in Hyloscirtus zu ändern und legte zwei neue Arten von Schlangen, Microsoma notatum und Liophis ygraecum. Sitzungsberichte der Gesellschaft Naturforschender Freunde zu Berlin, 1882, 127–129.

Pyron, R.A. & Wiens, J.J. (2011) A large-scale phylogeny of Amphibia including over 2800 species, and a revised classification of extant frogs, salamanders, and caecilians. Molecular Phylogenetics and Evolution, 61, 543–583.

Rivera-Correa, M. & Faivovich, J. (2014) Hyloscirtus palmeri (Boulenger 1908). Catálogo de los Anfibios y Reptiles de Colombia, 2, 1–6.

Rivera-Correa, M., García-Burneo, K. & Grant, T. (2016) A new red-eyed of stream treefrog of Hyloscirtus (Anura: Hylidae) from Peru, with comments on the taxonomy of the genus. Zootaxa, 4061 (1), 029–040.

Rivero, J.A. (1961) Salientia of Venezuela. Bulletin of the Museum of Comparative Zoology, 126, 1–207.

Rivero, J.A. (1968) Los centrolenidos de Venezuela (Amphibia, Salientia). Memoria de la Sociedad de Ciencias Naturales La Salle, 28, 301–334.

Rivero, J.A. (1970 “1969”) A new species of Hyla (Amphibia, Salientia) from the region of Paramo de Tama, Venezuela. Caribbean Journal of Science, 9, 145–150.

Rojas-Runjaic, F.J.M., Infante-Rivero, E.E. & Barrio-Amorós, C.L. (2011) A new frog of the genus Aromobates (Anura, Dendrobatidae) from Sierra de Perijá, Venezuela. Zootaxa, 2919, 37–50.

Rojas-Runjaic, F.J.M., Infante-Rivero, E.E. & Barrio-Amorós, C.L. (2016) New records, range extension and call description for the stream-breeding frog Hyloscirtus lascinius (Rivero, 1970) in Venezuela. Amphibian & Reptile Conservation, 10, 34–39. [e130]

Romero, J.H. & J.D. Lynch (2012) Anfibios de la Región Caribe. In: Rangel-Ch., J.O. (Ed.), Colombia Diversidad Biótica. XII. La región Caribe de Colombia. Universidad Nacional de Colombia, Instituto de Ciencias Naturales, Bogotá, D.C., pp. 677–701.

Ron, S.R. & Read, M. (2011) Hyloscirtus palmeri. In: Ron, S.R., Guayasamin, J.M., Yanez-Muñoz, M.H., Merino-Viteri, A., Ortiz, D.A. & Nicolalde, D.A. (2016) AmphibiaWebEcuador. Version 2016.0. Museo de Zoología, Pontificia Universidad Católica del Ecuador. Available from: (accessed 4 March 2016)

Ruiz-Carranza, P.M. & Lynch, J.D. (1982) Dos nuevas especies de Hyla (Amphibia: Anura) de Colombia, con aportes al conocimiento de Hyla bogotensis. Caldasia, 13, 647–671.

Ruiz-Carranza, P.M. & Ardila-Robayo, M.C. (1991) Una nueva especie de Hyla del grupo bogotensis (Amphibia: Anura: Hylidae) de la Cordillera Oriental de Colombia. Caldasia, 16, 337–342.

Salerno, P.E., Ron, S.R., Señaris, J.C., Rojas-Runjaic, F.J.M., Noonan, B.P. & Cannatella, D.C. (2012) Ancient tepui summits harbor young rather than old lineages of endemic frogs. Evolution, 66, 3000–3013.

Sánchez, D.A. (2010) Larval development and synapomorphies for species groups of Hyloscirtus Peters, 1882 (Anura: Hylidae: Cophomantini). Copeia, 2010, 351–363.

Savage, J.M. & Heyer, W.R. (1967) Variation and distribution in the tree-frog genus Phyllomedusa in Costa Rica, Central America. Beitrage zur Neotropischen Fauna, 5, 111–131.

Sievers, F., Wilm, A., Dineen, D., Gibson, T.J., Karplus, K., Li, W., Lopez, R., McWilliam, H., Remmert, M., Söding, J., Thompson, J.D. & Higgins, D.G. (2011) Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega. Molecular Systems Biology, 7, 539.

Sukumaran, J. & Holder, M.T. (2010) DendroPy: a Python library for phylogenetic computing. Bioinformatics 26, 1569–1571.

Sukumaran, J. & Holder, M.T. (2017) SumTrees: Phylogenetic Tree Summaritation. 4.3.0. Available from: (accessed 6 July 2017)

Vaidya, G., Lohman, D. J. & Meier, R. (2011) SequenceMatrix: concatenation software for the fast assembly of multigene datasets with character set and codon information. Cladistics, 27, 171–180.

Wiens, J.J., Kuczynski, C.A., Hua, X. & Moen, D.S. (2010) An expanded phylogeny of treefrogs (Hylidae) based on nuclear and mitochondrial sequence data. Molecular Phylogenetics and Evolution, 55, 871–882.



  • There are currently no refbacks.

ISSN 1175-5326 (Print Edition) & ISSN 1175-5334 (Online Edition)
Published by Magnolia Press, Auckland, New Zealand