Open Access Open Access  Restricted Access Subscription or Fee Access

A new Reed Frog (Hyperoliidae: Hyperolius) from coastal northeastern Mozambique

WERNER CONRADIE, LUKE VERBURGT, DANIEL M. PORTIK, ANNEMARIE OHLER, BERYL A. BWONG, LUCINDA P. LAWSON

Abstract


A new species of African reed frog (genus Hyperolius Rapp, 1842) is described from the Coastal Forests of the Eastern Africa Biodiversity Hotspot in northeastern Mozambique. It is currently only known from less than ten localities associated with the Mozambican coastal pans system, but may also occur in the southeastern corner of Tanzania. Phylogenetic reconstructions using the mitochondrial 16S marker revealed that it is the sister taxon of Hyperolius mitchelli (>5.6% 16S mtDNA sequence divergence) and forms part of a larger H. mitchelli complex with H. mitchelli and H. rubrovermiculatus. The new species is distinguished from other closely related Hyperolius species by genetic divergence, morphology, vocalisation, and dorsal colouration.

 


Keywords


Amphibia, Amphibian, endemic, coastal pans

Full Text:

PDF (13MB)

References


Aljanabi, S. & Martinez, I. (1997) Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques. Nucleic Acids Research, 25, 4692–4693.
https://doi.org/10.1093/nar/25.22.4692

Bayliss, J., Timberlake, J., Branch, W.R., Bruessow, C., Collins, S., Congdon, C., Curran, M., De Sousa, C., Dowsett, R., Dowsett-Lemaire, F., Fishpool, L., Harris, T., Herrmann, E., Georgiadis, S., Kopp, M., Liggitt, B., Monadjem, A., Patel, H., Ribeiro, D., Spottiswoode, C., Taylor, P., Willcock, S. & Smith, P. (2014) The discovery, biodiversity and conservation of Mabu forest—the largest mid-elevation rainforest in southern Africa. Oryx, 48, 177–185.
https://doi.org/10.1017/S0030605313000720

Bittencourt-Silva, G., Conradie, W., Siu-Ting, K., Tolley, K.A., Channing, A., Cunningham, M., Farooq, H.M., Menegon, M. & Loader, S.P. (2016) The phylogenetic position and diversity of the enigmatic mongrel frog Nothophryne Poynton, 1963 (Amphibia, Anura). Molecular Phylogenies and Evolution, 99, 89–102.
https://doi.org/10.1016/j.ympev.2016.03.021

Box, G. & Cox, D. (1964) An analysis of transformations. Journal of the Royal Statistical Society, Series B (Methodological), 26, 211–252.

Branch, W.R. & Bayliss, J. (2009) A new species of Atheris (Serpentes: Viperidae) from northern Mozambique. Zootaxa, 2113, 41–54.

Branch, W.R. & Tolley, K.A. (2010) A new species of chameleon (Sauria: Chamaeleonidae: Nadzikambia) from Mount Mabu, central Mozambique. African Journal of Herpetology, 59, 157–172.
https://doi.org/10.1080/21564574.2010.516275

Branch, W.R., Bayliss, J. & Tolley, K.A. (2014) Pygmy chameleons of the Rhampholeon platyceps complex (Squamata: Chamaeleonidae): Description of four new species from isolated ‘sky islands’ of northern Mozambique. Zootaxa, 3814 (1), 1–36.
https://doi.org/10.11646/zootaxa.3814.1.1

Branch, W.R., Rödel, M-O. & Marais, J. (2005) Herpetological survey of the Niassa Game Reserve, northern Mozambique—Part I: Reptiles. Salamandra, 41, 195–214.

Broadley, D.G. & Measey, G.J. (2016) A new species of Zygaspis (Reptilia: Squamata: Amphisbaenidae) from north-eastern Mozambique. African Journal of Herpetology, 65, 115–122.
https://doi.org/10.1080/21564574.2016.1183717

Burgess, N. & Clarke, G.P. (Eds.) (2000) Coastal Forests of Eastern Africa. IUCN Forest Conservation Programme, Gland & Cambridge, xiii + 443 pp.

Clarke, G.P. (2011) Observations on the vegetation and ecology of Palma and Nangade Districts, Cabo Delgado Province, Mozambique. Available from: http://coastalforests.org/ (accessed 1 August 2016)

Conradie, W., Bittencourt-Silva, G. Engelbrecht, H.M., Loader, S. P., Menegon, M., Nanvonamuquitxo, C., Scott, M. & Tolley, K.A. (2016) Exploration into the hidden world of Mozambique’s sky island forests: new discoveries of reptiles and amphibians. Zoosystematics and Evolution, 92, 163–180.
https://doi.org/10.3897/zse.92.9948

Conradie, W., Bittencourt-Silva, G., Farooq, H.M., Loader, S.P., Menegon, M. & Tolley, K.A. (2017) New species of Mongrel Frogs (Pyxicephalidae: Nothophryne) for northern Mozambique inselbergs. African Journal of Herpetology. [in press]

Daniels, S.R. & Bayliss, J. (2012) Neglected refugia of biodiversity: Mountainous regions in Mozambique and Malawi yield two novel freshwater crab species (Potamonautidae: Potamonautes). Zoological Journal of the Linnean Society, 164, 498–509.
https://doi.org/10.1111/j.1096-3642.2011.00773.x

Dehling, J.M. (2012) An African glass frog: A new Hyperolius species (Anura: Hyperoliidae) from Nyungwe National Park, southern Rwanda. Zootaxa, 3391, 52–64.

Drummond, A.J., Ashton, B., Buxton, S., Cheung, M., Cooper, A., Duran, C., Field, M., Heled, J., Kearse, M., Markowitz, S., Moir, R., Stones-Havas, S., Sturrock, S., Thierer, T. & Wilson, A. (2011) Geneious v5.4. Available from: http://www.geneious.com (accessed 19 December 2017)

Farooq, H.O.M. & Conradie, W. (2015) A second record of Scolecomorphus kirkii Boulenger, 1883 (Gymnophiona: Scolecomorphidae) for Mozambique. Herpetology Notes, 8, 59–62.

Farooq, H., Liedtke, H.C., Bittencourt-Silva, G., Conradie, W. & Loader, S. (2015) The distribution of Mertensophryne anotis with a new record in Northern Mozambique. Herpetology Notes, 8, 305–307.

Gerhardt, H.C. & Huber, F. (2002) Acoustic communication in insects and anurans: common problems and diverse solutions. University of Chicago Press, Chicago, 544 pp.

Heyer, W.R., Rand, A.S., Dacruz, C.A.G., Peixoto, O.L. & Nelson, C.E. (1990) Frogs of Boracéia. Arquivos de Zoología, 31, 237–410.

IUCN (2012) IUCN Red List Categories and Criteria: Version 3.1. 2nd Edition. IUCN, Gland & Cambridge, 32 pp.

Katoh, K. & Standley, D.M. (2013) MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability. Molecular Biology and Evolution, 30, 722–780.
https://doi.org/10.1093/molbev/mst010

Köhler, J., Scheelke, K., Schick, S., Veith, M. & Lötters, S. (2005) Contribution to the taxonomy of hyperoliid frogs (Amphibia: Anura: Hyperoliidae): advertisement calls of twelve species from East and Central Africa. African Zoology, 40 (1), 127–142.

Köhler, J., Jansen, M., Rodriguez, A., Kok, P.J., Toledo, L.F., Emmrich, M., Glaw, F., Haddad, C.F., Rödel, M.O. & Vences, M. (2017) The use of bioacoustics in anuran taxonomy: theory, terminology, methods and recommendations for best practice. Zootaxa, 4251 (1), 1–124.
https://doi.org/10.11646/zootaxa.4251.1.1

Kumar, S., Stecher, G. & Tamura, K. ( 2016) MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets . Molecular Biology and Evolution, 33, 1870–1874.
https://doi.org/10.1093/molbev/msw054

Ohler, A. & Frétey, T. (2014) Going back to Rovuma: The frog fauna of a coastal dry forest, and a checklist of the Amphibians of Mozambique. Journal of East African Natural History, 103 (2), 73–124.
https://doi.org/10.2982/028.103.0203

Palumbi, S., Martin, A., Romano, S., McMillan, W.O., Stice, L. & Grabowski, G. (1991) The Simple Fool’s Guide to PCR. Version 2. University of Hawaii, Honolulu, 45 pp.

Pascal, O. (2011) The Coastal Forests of Northern Mozambique, 2008 - 2009 expeditions. Our Planet Reviewed. Programme report n°1. Pro-Natura international / Muséum national d’Histoire naturelle, Paris. Available from: http://www.laplaneterevisitee.org/ressources/pdfs/Rapport_de_Mission_Mozambique_23.pdf (accessed 20 July 2016)

Portik, D.M. (2015) Diversification of Afrobatrachian frogs and the herpetofauna of the Arabian Peninsula. Ph.D. Thesis. Department of Integrative Biology, University of California, Berkeley, 209 pp.

Portik, D.M., Mulungu, E.A., Sequeira, D. & McEntee, J.P. (2013a) Herpetological surveys of the Serra Jeci and Namuli massifs, Mozambique, and an annotated checklist of the southern Afromontane archipelago. Herpetological Review, 44, 394–406.

Portik, D.M., Travers, S.L., Bauer, A.A. & Branch, W.R. (2013b) A new species of Lygodactylus (Squamata: Gekkonidae) endemic to Mount Namuli, an isolated ‘sky island’ of northern Mozambique. Zootaxa, 3710 (5), 415–435.
https://doi.org/10.11646/zootaxa.3710.5.2

Poynton, J.C. & Broadley, D.G. (1987) Amphibia Zambesiaca 3. Rhacophoridae and Hyperoliidae. Annals of the Natal Museum, 28 (1), 161–229.

R Development Core Team (2014) R: A language and environment for statistical computing. R Foundation for Statistical Computing Vienna, Austria. Available from: http://www.R-project.org (accessed 19 December 2017)

Rödel, M.-O. (2000) Herpetofauna of West Africa, Vol. I: Amphibians of the West African savanna. Edition Chimaira, Frankfurt/M., 322 pp.

Schiøtz, A. (1999) Treefrogs of Africa. Edition Chimaira, Frankfurt/M., 350 pp.

Spieler, M. & Linsenmair, K.E. (1998) Migration patterns and diurnal use of shelter in a ranid frog of a West African savannah: a telemetric study. Amphibia-Reptilia, 19, 43–64.
https://doi.org/10.1163/156853898X00322

Stamatakis, A. (2014) RAxML version 8: a tool for phylogenetic analysis and postanalysis of large phylogenies. Bioinformatics, 30, 1312–1313.
https://doi.org/10.1093/bioinformatics/btu033

Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. & Kumar, S. (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28, 2731–2739.
https://doi.org/10.1093/molbev/msr121

Taylor, P.J., Stoffberg, S., Monadjem, A., Schoeman, M.C., Bayliss, J. & Cotterill, F.P.D. (2012) Four new bat species (Rhinolophus hildebrandtii complex) reflect Plio-Pleistocene divergence of dwarfs and giants across an afromontane archipelago. PloS ONE, 7, e41744.
https://doi.org/10.1371/journal.pone.0041744

Timberlake J., Bayliss, J., Alves, T., Baena, S., Francisco, J., Harris, T. & da Sousa, C. (2007) Biodiversity and conservation of Mount Chiperone, Mozambique. Technical Report: Darwin Initiative Award 15/036: Monitoring and Managing Biodiversity Loss in South-East Africa’s Montane Ecosystems. Available from: http://www.kew.org/sites/default/files/kppcont_046092_0.pdf (accessed 20 July 2016)

Timberlake, J., Dowsett-Lemaire, F., Bayliss, J., Alves, T., Baena, S., Bento, C., Cook, K., Francisco, J., Harris, T., Smith, P. & de Sousa, C. (2009) Mt. Namuli. Mozambique: Biodiversity and conservation. Technical Report: Darwin Initiative Award 15/036: Monitoring and Managing Biodiversity Loss in South-East Africa’s Montane Ecosystems. Available from: http://www.kew.org/science/directory/projects/annex/Namuli_report_FINAL.pdf (accessed 20 July 2016)

Timberlake, J., Goyder, D., Crawford, F. & Pascal, O. (2010) Coastal dry forests in Cabo Delgado Province, Northern Mozambique—botany & vegetation. Report for "Our Planet Reviewed" a joint initiative Pro- Natura international and the French Museum of Natural History, Royal Botanic Gardens, Kew, London. Available from: http://www.kew.org/sites/default/files/kppcont_046087-1_0.pdf (accessed 1 August 2016).

Timberlake, J., Goyder, D., Crawford, F., Burrows, J., Clarke, G.P., Luke, Q., Matimele, H., Muller, T. Pascal, O., De Sousae & Alves, T. (2011) Coastal dry forests in northern Mozambique. Plant Ecology and Evolution, 144, 126–137.
https://doi.org/10.5091/plecevo.2011.539

Timberlake, J.R., Bayliss, J., Dowsett-Lemaire, F., Conghan, C., Branch, W.R., Collins, S., Curran, M., Dowsett, R.J., Fishpool, L., Francisco, J., Harris, T., Kopp, M. & de Sousa, C. (2012) Mt. Mabu, Mozambique: Biodiversity and conservation. Report produced under the Darwin Initiative Award 15/036. Royal Botanic Gardens, Kew, London. Available from: http://www.kew.org/sites/default/files/kppcont_067897.pdf (accessed 26 January 2015)

Verburgt, L. (2013) Herpetofauna baseline of the Afungi Peninsula. In: Environmental Impact Assessment - I) Introduction, project description and baseline. Anadarko Petroleum Corporation, Texas, USA. Available from: http://www.mzlng.com/content/documents/MZLNG/EIA/Volume_I/English/Chapter_8-_LNG_Final_EIA_Feb_2014_Eng.pdf (accessed 19 July 2016)

Verburgt, L., Ferreira, M. & Ferguson, J.W.H. (2011) Male field cricket song reflects age, allowing females to prefer young males. Animal Behavior, 81, 19–29.
https://doi.org/10.1016/j.anbehav.2010.09.010

Verburgt, L., Verburgt, U.K. & Branch, W.R. (2017) A new species of Scolecoseps (Reptilia: Scincidae) from coastal north-eastern Mozambique. African Journal of Herpetology. [in Press]

Watters, J.L., Cummings, S.T., Flanagan, R.L. & Siler, C.D. (2016) Review of morphometric measurements used in anuran species descriptions and recommendations for a standardized approaches. Zootaxa, 4072 (4), 477–495.
https://doi.org/10.11646/zootaxa.4072.4.6

Wickham, H. (2009) Ggplot2: Elegant Graphics for Data Analysis. Springer-Verlag, New York. 213 pp.
https://doi.org/10.1007/978-0-387-98141-3




DOI: http://dx.doi.org/10.11646/zootaxa.4379.2.2

Refbacks

  • There are currently no refbacks.


 
ISSN 1175-5326 (Print Edition) & ISSN 1175-5334 (Online Edition)
Published by Magnolia Press, Auckland, New Zealand