Skip to main content Skip to main navigation menu Skip to site footer
Type: Article
Published: 2017-12-12
Page range: 553–560
Abstract views: 80
PDF downloaded: 2

Identification of Neoceratitis asiatica (Becker) (Diptera: Tephritidae) based on morphological characteristics and DNA barcode

Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China
Ningxia Academy of Agriculture and Forestry Science, Yinchuan 750002, China
Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China
Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China
Ningxia Academy of Agriculture and Forestry Science, Yinchuan 750002, China
Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China Ningxia Academy of Agriculture and Forestry Science, Yinchuan 750002, China
Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China
Ningxia Academy of Agriculture and Forestry Science, Yinchuan 750002, China
Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China
Diptera Neoceratitis asiatica (Becker) identification morphological characteristics DNA barcode COI

Abstract

Neoceratitis asiatica (Becker), which especially infests wolfberry (Lycium barbarum L.), could cause serious economic losses every year in China, especially to organic wolfberry production. In some important wolfberry plantings, it is difficult and time-consuming to rear the larvae or pupae to adults for morphological identification. Molecular identification based on DNA barcode is a solution to the problem. In this study, 15 samples were collected from Ningxia, China. Among them, five adults were identified according to their morphological characteristics. The utility of mitochondrial DNA (mtDNA) cytochrome c oxidase I (COI) gene sequence as DNA barcode in distinguishing N. asiatica was evaluated by analysing Kimura 2-parameter distances and phylogenetic trees. There were significant differences between intra-specific and inter-specific genetic distances according to the barcoding gap analysis. The uncertain larval and pupal samples were within the same cluster as N. asiatica adults and formed sister cluster to N. cyanescens. A combination of morphological and molecular methods enabled accurate identification of N. asiatica. This is the first study using DNA barcode to identify N. asiatica and the obtained DNA sequences will be added to the DNA barcode database.

 

References

  1. Armstrong, K.F. & Ball, S.L. (2005) DNA barcodes for biosecurity: invasive species identification. Philosophical Transactions of the Royal Society B, 360, 1813–1823.
    https://doi.org/10.1098/rstb.2005.1713

    Bai, H., Wei, X.T., Liang, W., Ni, X.M., Zhang, J.X. & Song, T. (2011) Rapid identification of Cydia pomonella L by Real-Time PCR. Plant Quarantine, 25, 48–51.

    Ball, S.L., Hebert, P.D.N., Burian, S.K. & Webb, J.M. (2005) Biological identifications of mayflies (Ephemeroptera) using DNA barcodes. Journal of the North American Benthological Society, 24, 508–524.
    https://doi.org/10.1899/04-142.1

    Bertin, S., Picciau, L., Acs, Z., Alma, A. & Bosco, D. (2010) Molecular differentiation of four Reptalus species (Hemiptera: Cixiidae). Bulletin of Entomological Research, 100, 551–558.
    https://doi.org/10.1017/S0007485309990605

    Boge, A., Gerstmeier, R. & Einspanier, R. (1994) Molecular polymorphism as a tool for differentiating ground beetles (Carabus species): Application of ubiquitin PCR/SSCP analysis. Insect Molecular Biology, 3, 267–271.
    https://doi.org/10.1111/j.1365-2583.1994.tb00176.x

    Botstein, D., White, R.L., Skolnick, M. & Davis, R.W. (1980) Construction of a genetic-linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics, 32, 314–331.

    Cywinska, A., Hunter, F.F. & Hebert, P.D.N. (2006) Identifying Canadian mosquito species through DNA barcodes. Medical and Veterinary Entomology, 20, 413–424.
    https://doi.org/10.1111/j.1365-2915.2006.00653.x

    Cywinska, A., Hannan, M.A., Kevan, P.G., Roughley, R.E., Iranpour, M. & Hunter, F.F. (2010) Evaluation of DNA barcoding and identification of new haplomorphs in Canadian deerflies and horseflies. Medical and Veterinary Entomology, 24, 382–410.
    https://doi.org/10.1111/j.1365-2915.2010.00896.x

    Felsenstein, J. (1985) Confidence-limits on phylogenies- an approach using the bootstrap. Evolution, 39, 783–791.
    https://doi.org/10.2307/2408678

    Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3, 294–299.

    Greenstone, M.H., Rowley, D.L., Heimbach, U., Lundgren, J.G., Pfannenstiel, R.S. & Rehner, S.A. (2005) Barcoding generalist predators by polymerase chain reaction: carabids and spiders. Molecular Ecology, 14, 3247–3266.
    https://doi.org/10.1111/j.1365-294X.2005.02628.x

    Hajibabaei, M., Janzen, D.H., Burns, J.M., Hallwachs, W. & Hebert, P.D.N. (2006) DNA barcodes distinguish species of tropical Lepidoptera. Proceedings of the National Academy of Sciences of the United States of America, 103, 968–971.
    https://doi.org/10.1073/pnas.0510466103

    Hebert, P.D.N, Cywinska, A., Ball, S.L. & deWaard, J.R. (2003a) Biological identifications through DNA barcodes. Proceedings of the Royal Society B-Biological Sciences, 270, 313–321.

    https://doi.org/10.1098/rspb.2002.2218

    Hebert, P.D.N., Ratnasingham, S. & deWaard, J.R. (2003b) Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proceedings of the Royal Society B-Biological Sciences, 270, S96–S99.
    https://doi.org/10.1098/rsbl.2003.0025

    Janzen, D.H., Hajibabaei, M., Burns, J.M., Hallwachs, W., Remigio, E. & Hebert, P.D.N. (2005) Wedding biodiversity inventory of a large and complex Lepidoptera fauna with DNA barcoding. Proceedings of the Royal Society B-Biological Sciences, 360, 1835–1845.
    https://doi.org/10.1098/rstb.2005.1715

    Jiang, F., Jin, Q., Liang, L., Zhang, A.B. & Li, Z.H. (2014a) Existence of species complex largely reduced barcoding success for invasive species of Tephritidae: a case study in Bactrocera spp. Molecular Ecology Resources, 14, 1114–1128.
    https://doi.org/10.1111/1755-0998.12259

    Jiang, F., Li, Z.H., Deng, Y.L., Wu, J.J., Liu, R.S. & Buahom, N. (2013) Rapid diagnosis of the economically important fruit fly, Bactrocera correcta (Diptera: Tephritidae) based on a species-specific barcoding cytochrome oxidase I marker. Bulletin of Entomological Research, 103, 363–371.

    https://doi.org/10.1017/S0007485312000806

    Jiang, F., Li, Z.H., Wu, J.J., Wang, F.X. & Xiong, H.L. (2014b) A rapid diagnostic tool for two species of Tetradacus (Diptera: Tephritidae: Bactrocera) based on species-specific PCR. Journal of Applied Entomology, 138, 418–422.

    https://doi.org/10.1111/jen.12041

    Kimura, M. (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide-sequences. Journal of Molecular Evolution, 16, 111–120.

    https://doi.org/10.1007/BF01731581

    Kumar, S., Stecher, G. & Tamura, K. (2016) MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Molecular Biology and Evolution, 33, 1870–1874.

    https://doi.org/10.1093/molbev/msw054

    Li, S.Z., Ma, Y.J. & Zheng, Z.M. (2003) Molecular Identification of Anopheles maculatus Complex (Diptera: Culicidae) from China: Single-strand Conformation Polymorphism (SSCP) Assay. Entomotaxonomia, 25, 125–130.

    Liu, L.J., Liu, J.Q., Wang, Q.L., Ndayiragije, P., Ntahimpera, A., Nkubaye, E., Yang, Q.Q. & Li, Z.H. (2011) Identification of Bactrocera invadens (Diptera: Tephritidae) from Burundi, based on morphological characteristics and DNA barcode. African Journal of Biotechnology, 10, 13623–13630.

    Peng, X.Z., Sun, L., Guo, Y., Yang, X.H. & Shen, X.R. (2000) The occurrence and prevention of three pests of Chinese wolfberry. Inner Mongolia Agricultural Science and Technology, 6, 13–14.

    Qin, L., Wang, J., Bing, X.L. & Liu, S.S. (2013) Identification of nine cryptic species of Bemisia tabaci (Hemiptera: Aleyrodidae) from China by using the mtCOI PCR-RFLP technique. Acta Entomologica Sinica, 56, 186–194.

    Ratnasingham, S. & Hebert, P.D.N. (2007) The Barcode of Life Data System. Molecular Ecology Notes, 7, 335–364. [http://www.barcodinglife.org]

    Smith, M.A., Fisher, B.L. & Hebert, P.D.N. (2005) DNA barcoding for effective biodiversity assessment of a hyperdiverse arthropod group: the ants of Madagascar. Philosophical Transactions of the Royal Society B-Biological Sciences, 360, 1825–1834.

    https://doi.org/10.1098/rstb.2005.1714

    Wang, C.F. (2015) The identification and prevention of main pests and diseases of Chinese wolfberry. Agriculture and Technology, 35, 132–133.

    Wang, X.J. (1996) The fruit flies (Diptera: Tephritidae) of the East Asian region. Acta Zootaxonomica Sinica, 21 (Supplement), 1–338.

    Wilkerson, R.C., Parsons, T.J., Klein, T.A., Gaffigan, T.V., Bergo, E. & Consolim, J. (1995) Diagnosis by random amplified polymorphic DNA-polymerase chain reaction of four cryptic species-related to anopheles (Nyssorhynchus) albitarsis (Diptera, Culicidae) from Paraguay, Argentina, and Brazil. Journal of Medical Entomology, 32, 697–704.
    https://doi.org/10.1093/jmedent/32.5.697

    Wu, F.Z. & Meng, Q.X. (1963) Distribution, damage, life habit and prevention and control method of Neoceratitis asiatica (Becker). Ningxia agricultural science communication, 6, 35–37.

    Wu, F.Z., Huang, R.X. Meng, Q.X. & Liang, Z.Q. (1963) Studies on the life history and the control of lycium fruit fly Neoceratitis asiatica (Becker) (Diptera, Trypetidae). Journal of Plant Protection, 2, 387–398.

    Xu, L., Yu, D.J., Jiao, Y., Wang, Y.Z. & Chen, Z.L. (2010) TaqMan Real-time qualitative PCR for the inspection and identification of Planococcus minor and P. lilacius (Homoptera: Pseudococcidae). Plant Quarantine, 24, 24–28.

    Xue, F.X. (2009) The identification and prevention of main pests and diseases of Chinese wolfberry. Gansu science and technology, 38, 52–53, 135.

    Xue, F.X. & Lin, H.M. (2009) Problems in prevention strategy and control of diseases and pests of Chinese wolfberry. Gansu science and technology, 38, 57–58.

    Yang, Q.Q., Zhao, S., Kucerova, Z., Opit, G., Cao, Y., Stejskal, V. & Li, Z.H. (2013) Rapid molecular diagnosis of the stored-product psocid Liposcelis corrodens (Psocodea: Liposcelididae): Species-specific PCR primers of 16S rDNA and COI. Journal of Stored Products Research, 54, 1–7.
    https://doi.org/10.1016/j.jspr.2013.03.005

    Zhang, H.M. (2004) Molecular identification in four species of fruit flies (Diptera: Tephritidae) distributed commonly in Shaanxi province. Thesis, Northwest Sci-Tech University of Agriculture and Forestry, Yangling, 48 pp.

    Zhang, X. (2016) Identification and prevention of common diseases and insect pests of Chinese wolfberry. China Forestry Industry, 11, 276.

    Zheng, Q.W. (2015) Preliminary progress has been made in the development of the control technology of Chinese wolfberry in Ningxia. Pesticide Market News, 26, 66.