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A new species of the genus Microhyla (Anura: Microhylidae) from Eastern Nepal 

JANAK RAJ KHATIWADA, GUO CHENG SHU, SHOU HONG WANG, ARJUN THAPA, BIN WANG, JIANPING JIANG

Abstract


A new species of the genus Microhyla is described from Jamun Khadi, Jhapa district of eastern Nepal, based on molecular and morphological comparisons. This species is the sister taxon of Microhyla ornata and can be distinguished by a unique vocalization, morphology and molecular phylogeny. The uncorrected genetic divergences based on rRNA gene between the new species and its closest congeners, M. nilphamariensis, M. ornata and M. rubra were 5.34%, 6.67%, and 8.31%, respectively. The new species, Microhyla taraiensis sp. nov., is distinguished from each other of Microhyla by a combination of the following morphological characters: (1) relatively larger body size (SVL ranges 19.9–20.3 mm, n = 4 in the males and 22.1–24.9 mm, n = 3 in the females); (2) dorsal surface of head and body with light red dots; (3) toes webbing poorly developed or absent; (4) a large round inner metacarpal tubercle; and an (5) elongated outer metacarpal tubercle. In addition, our study also provides a new record of Microhyla nilphamariensis from Nepal.

 


Keywords


Amphibia, Microhyla taraiensis sp. nov., Range extension, Microhyla nilphamariensis, Molecular phylogeny, Taxonomy

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References


Al Haidar, I.K., Rahman, M., Ahsan, M.F., Islam, A., Tania, J., Banick, P.R. & Ahmed, S. (2014) Records of three new butterfly species from the Chittagong University Campus of Chittagong in Bangladesh. Entomology and Zoology Studies, 2, 178−181.

Andersson, L.G. (1942) A small collection of frogs from Annam collected in the years 1938–1939 by Bertil Björkegren. Arkiv för Zoologi, 34, 1−11.

Bain, R.H. & Nguyen, T.Q. (2004) Three new species of narrow-mouthed frogs (genus Microhyla) from Indochina, with comments on Microhyla annamensis and Microhyla palmipes. Copeia, 2004, 507–524.
https://doi.org/10.1643/CH-04-020R2

Biju, S.D., Senevirathne, G., Garg, S., Mahony, S., Kamei, R.G., Thomas, A., Shouche, Y. Raxworthy, C.J., Meegaskumbura, M. & van Bocxlaer, I. (2016) Frankixalus, a new rhacophorid genus of tree hole breeding frogs with oophagous tadpoles. PloS ONE, 11, e0145727.
https://doi.org/10.1371/journal.pone.0145727

Blyth, E. (1856) Report for October meeting, 1855. Journal of the Asiatic Society of Bengal, 24, 711−723.

Boulenger, G.A. (1884) Descriptions of new species of reptiles and batrachians in the British Museum - Part II. Annals and Magazine of Natural History, Series 5, 13 (77), 396−398.
https://doi.org/10.1080/00222938409459259

Boulenger, G.A. (1897) Descriptions of new Malay frogs. Annals and Magazine of Natural History, 6, 106–108.
https://doi.org/10.1080/00222932008632416

Boulenger, G.A. (1900) Descriptions of New Batrachians and Reptiles from Larut Hills, Perak. Annales Magazine of Natural History, 6, 186−193.
https://doi.org/10.1080/00222930008678356

Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature Methods, 9, 772−772.
https://doi.org/10.1038/nmeth.2109

Das, I. & Haas, A. (2010) New species of Microhyla from Sarawak: Old World’s smallest frogs crawl out of miniature pitcher plants on Borneo (Amphibia: Anura: Microhylidae). Zootaxa, 2571, 37−52.

Das, I., Yaakob, N. & Sukumaran, J. (2007) A new species of Microhyla (Anura: Microhylidae) from the Malay Peninsula. Hamadryad, 31, 304−314.

Dobremez, J.F. (1976) Le Népal: Écologie et Biogéographie. Centre National de la Recherche Scientifique, Paris, 355 pp.

Duméril, A.M.C. & Bibron, G. (1841) Erpétologie Genérale ou Histoire Naturelle Complète des Reptiles. Tome 8. Librarie Enclyclopedique de Roret, Paris, iii + 792 pp.

Dutta, A.K., Paloi, S., Pradhan, P. & Acharya, K. (2015) A new species of Russula (Russulaceae) from India based on morphological and molecular (ITS sequence) data. Turkish Journal of Botany, 39, 850−856.
https://doi.org/10.3906/bot-1407-1

Dutta, S. & Ray, P. (2000) Microhyla sholigari, a new species of microhylid frog (Anura: Microhylidae) from Karnataka, India. Hamadryad, 25, 38−44.

Fei, L., Ye, C.Y. & Jiang, J.P. (2012) Colored Atlas of Chinese Amphibians and Their Distributions. Sichuan Publishing House of Science and Technology, Chengdu, 510 pp. [in Chinese]

Fei, L., Ye, C.Y., Jiang, J.P., Xie, F. & Huang, Y.Z. (2005) An Illustrated Key to Chinese Amphibians. Sichuan Publishing Group and Sichuan Publishing House of Science and Technology, Chengdu, 340 pp. [in Chinese]

Fernando, P. & Siriwardhane, M. (1996) Microhyla karunaratnei (Anura: Microhylidae), a new species of frog endemic to Sri Lanka. Journal of South Asian Natural History, 2, 135−142.

Frost, D.R. (2017) Amphibian Species of the World 6.0. An Online Reference. American Museum of Natural History, New York. Available from http://research.amnh.org/vz/herpetology/amphibia/index.php (accessed 23 February 2016)

Hajibabaei, M., Singer, G.A., Hebert, P.D. & Hickey, D.A. (2007) DNA barcoding: how it complements taxonomy, molecular phylogenetics and population genetics. TRENDS in Genetics, 23, 167–172.
https://doi.org/10.1016/j.tig.2007.02.001

Hallowell, E. (1861) Report upon the Reptilia of the North Pacific Exploring Expedition, under command of Capt. John Rogers, U.S. N 1860. Proceedings of the Academy of Natural Sciences of Philadelphia, 12, 480−510.

Hasan, M., Islam, M.M., Kuramoto, M., Kurabayashi, A. & Sumida, M. (2014) Description of two new species of Microhyla (Anura: Microhylidae) from Bangladesh. Zootaxa, 3755 (5), 401−418.
https://doi.org/10.11646/zootaxa.3755.5.1

Hasan, M., Razzaque, M.A., Sarker, A.K., Kuramoto, M. & Sumida, M. (2015) Genetic variation, advertisement call, and morphometry of Microhyla nilphamariensis from Bangladesh. Philippine Journal of Systematic Biology, IX, 63–80.

Howlader, M.S.A., Nair, A., Gopalan, S.V. & Merilä, J. (2015a) A new species of Microhyla (Anura: Microhylidae) from Nilphamari, Bangladesh. PloS ONE, 10, e0119825.
https://doi.org/10.1371/journal.pone.0119825

Howlader, M.S.A., Nair, A., Gopalan, S.V. & Merilä, J. (2015b) A new species of Euphlyctis (Anura: Dicroglossidae) from Barisal, Bangladesh. PloS ONE, 10, e0116666.
https://doi.org/10.1371/journal.pone.0116666

Hu, S., Zhao, E. & Liu, C. (1966) A herpetological survey of the Tsinling and Ta-Pa Shan region. Acta Zoologica Sinica, 18, 57−89.

Huson, D.H. & Bryant, D. (2006) Application of phylogenetic networks in evolutionary studies. Molecular Biology and Evolution, 23, 254−267.
https://doi.org/10.1093/molbev/msj030

Inger, R.F. & Frogner, K.J. (1979) New species of narrow-mouth frogs (genus Microhyla) from Borneo. Sarawak Museum Journal, 27, 311−322.

Inger, R.F. (1989) Four new species of frogs from Borneo. Malayan Nature Journal, 229−243.

IUCN (2001) Guidelines for Using the IUCN Red List Categories and Criteria: Version 3.1. IUCN Species Survival Commission, Gland & Cambridge, iv + 32 pp.

Jerdon, T.C. (1853) Catalogue of reptiles inhabiting the peninsula of India. Journal of Asiatic Society Bengal, 22, 462−479.

Khatiwada, J.R., Wang, B., Ghimire, S., Vasudevan, K., Paudel, S. & Jiang, J. (2015) A New Species of the Genus Tylototriton (Amphibia: Urodela: Salamandridae) from Eastern Himalaya. Asian Herpetological Research, 6, 245−256.

Khatiwada, J.R., Ghimire, S., Khatiwada, S.P., Paudel, B., Bischof, R., Jiang, J. & Haugaasen, T. (2016) Frogs as potential biological control agents in the rice fields of Chitwan, Nepal. Agriculture, Ecosystems & Environment, 230, 307–314.
https://doi.org/10.1016/j.agee.2016.06.025

Kumar, S., Stecher, G. & Tamura, K. (2016) MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33, 1870–1874.
https://doi.org/10.1093/molbev/msw054

Kuramoto, M. & Joshy, S.H. (2006) Morphological and acoustic comparisons of Microhyla ornata, M. fissipes, and M. okinavensis (Anura: Microhylidae). Current Herpetology, 25, 15–27.
https://doi.org/10.3105/1345-5834(2006)25[15:MAACOM]2.0.CO;2

Leviton, A.E., Myers, G.S. & Swan, L.W. (1956) Zoological results of the California Himalayan Expedition to Makalu Eastern Nepal. In: Occasional papers of the Natural History Museum of Stanford University. No.1. Vol. 1. Amphibians and reptiles. Natural History Museum of Stanford University, Stanford, California, pp. 1–18.

Mahony, S., Teeling, E.C. & Biju, S. (2013) Three new species of horned frogs, Megophrys (Amphibia: Megophryidae), from northeast India, with a resolution to the identity of Megophrys boettgeri populations reported from the region. Zootaxa, 3722 (2), 143−169.
https://doi.org/10.11646/zootaxa.3722.2.2

Malkmus, R. (2002) Amphibians & Reptiles of Mount Kinabalu (North Borneo). Koeltz Scientific Books, Königstein, 424 pp.

Matsui, M. (2011) Taxonomic revision of one of the Old World's smallest frogs, with description of a new Bornean Microhyla (Amphibia, Microhylidae). Zootaxa, 2814 (1), 33−49.
https://doi.org/10.11646/zootaxa.2814.1.3

Matsui, M., Hamidy, A. & Eto, K. (2013) Description of a new species of Microhyla from Bali, Indonesia (Amphibia, Anura). Zootaxa, 3670 (4), 579−590.
https://doi.org/10.11646/zootaxa.3670.4.9

Matsui, M., Hamidy, A., Belabut, D.M., Ahmad, N., Panha, S., Sudin, A., Khonsue, W., Oh, H.-S., Yong, H.-S. & Jiang, J.P. (2011) Systematic relationships of Oriental tiny frogs of the family Microhylidae (Amphibia, Anura) as revealed by mtDNA genealogy. Molecular Phylogenetics and Evolution, 61, 167–176.
https://doi.org/10.1016/j.ympev.2011.05.015

Matsui, M., Ito, H., Shimada, T., Ota, H., Saidapur, S.K., Khonsue, W., Tanaka-Ueno, T. & Wu, G.F. (2005) Taxonomic relationships within the Pan-Oriental narrow-mouth toad Microhyla ornata as revealed by mtDNA analysis (Amphibia, Anura, Microhylidae). Zoological Science, 22, 489–495.
https://doi.org/10.2108/zsj.22.489

Mitchell, J. & Zug, G. (1995) Keys to the known amphibians and reptiles of the Royal Chitwan National Park, Nepal. Smithsonian Herpetological Information Service, 106, 1–15.
https://doi.org/10.5479/si.23317515.106.1

Myers, N., Mittermeier, R.A., Mittermeier, C.G., da Fonseca, G.A.B. & Kent, J. (2000) Biodiversity hotspots for conservation priorities. Nature, 403, 853–858.
https://doi.org/10.1038/35002501

Nanhoe, L.M. & Ouboter, P.E. (1987) The distribution of reptiles and amphibians in the Annapurna-Dhaulagiri region (Nepal). Rijksmuseum van Natuurlijke Historie, Leiden, 105 pp.

Olson, D.M., Dinerstein, E., Wikramanayake, E.D., Burgess, N.D., Powell, G.V., Underwood, E. C., D'amico, J.A., Itoua, I., Strand, H.E. & Morrison, J.C. (2001) Terrestrial ecoregions of the world: A new map of life on earth. BioScience, 51, 933−938.
https://doi.org/10.1641/0006-3568(2001)051[0933:teotwa]2.0.co;2

Parker, H. & Osman-Hill, W. (1948) Frogs of the genus Microhyla from Ceylon. Journal of Natural History, 1, 759−764.Parker, H. (1928) The brevicipitid frogs of the genus: Microhyla. Annals and Magazine of Natural History, 10, 473–499.

Peloso, P.L., Frost, D.R., Richards, S.J., Rodrigues, M.T., Donnellan, S., Matsui, M., Raxworthy, C.J., Biju, S., Lemmon, E.M. & Lemmon, A.R. (2015) The impact of anchored phylogenomics and taxon sampling on phylogenetic inference in narrow‐mouthed frogs (Anura, Microhylidae). Cladistics, 32, 113–140.
https://doi.org/10.1111/cla.12118

Pillai, R. (1977) On two frogs of the family Microhylidae from Andamans including a new species. Proceedings of the Indian Academy of Sciences, Section-B, 86, 135−138.

Poyarkov Jr., N.A., Vassilieva, A.B., Orlov, N.L., Galoyan, E.A., Tran, D., Le, D.T.T., Kretova, V.D. & Geissler, P. (2014) Taxonomy and distribution of narrow-mouth frogs of the genus Microhyla Tschudi, 1838 (Anura: Microhylidae) from Vietnam with descriptions of five new species. Russian. Journal of Herpetology, 21, 89−148.

R Core Team (2013) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna. Available from: https://www.r-project.org/ (accessed 20 March 2017)

Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572−1574.
https://doi.org/10.1093/bioinformatics/btg180

Schenkel, E. (1901) Achter Nachtrag zum Katalog der herpetologischen Sammlung des Basler Museums. Verhandlungen der Naturforschenden Gesellschaft in Basel, 13, 142–199.

Schleich, H.H. & Kästle, W. (2002) Amphibians and Reptiles of Nepal. Koeltz Scientific Books, Koenigstein, 1295 pp.

Seshadri, K., Singal, R., Priti, H., Ravikanth, G., Vidisha, M., Saurabh, S., Pratik, M. & Gururaja, K.V. (2016) Microhyla laterite sp. nov., a new species of Microhyla Tschudi, 1838 (Amphibia: Anura: Microhylidae) from a laterite rock formation in South West India. PloS ONE, 11, e0149727.
https://doi.org/10.1371/journal.pone.0149727

Shah, K.B. & Tiwari, S. (2004) Herpetofauna of Nepal: A conservation companion. IUCN Nepal, Kathmandu, Nepal, 237 pp.

Smith, M. & Batersby, J.C. (1953) On a collection of amphibians and reptiles rom Nepal. Annals and Magazine of Natural History, Series 12, 6, 702–704.
https://doi.org/10.1080/00222935308654472

Smith, M. (1923) Notes on reptiles and batrachians from Siam and Indo-China (No. 2). Journal of the Natural History Society of Siam, 6, 47−53.

Smith, M. & Batersby, J.C. (1953) On a collection of amphibians and reptiles rom Nepal. Annals and Magazine of Natural History, Series 12, 6, 702–704.

Smith, M. (1951) On a collection of amphibians and reptiles from Nepal. The Annals & Magazine of Natural History, 4, 726–728.
https://doi.org/10.1080/00222935108654199

Stejneger, L. (1901) Diagnoses of eight new batrachians and reptiles from the Riu Kiu Archipelago, Japan. Proceedings of the Biological Society of Washington, 14, 189−191.

Sumida, M., Kotaki, M., Islam, M.M., Djong, T.H., Igawa, T., Kondo, Y., Matsui, M., Khonsue, W. & Nishioka, M. (2007) Evolutionary relationships and reproductive isolating mechanisms in the rice frog (Fejervarya limnocharis) species complex from Sri Lanka, Thailand, Taiwan and Japan, inferred from mtDNA gene sequences, allozymes, and crossing experiments. Zoological Science, 24, 547–562.
https://doi.org/10.2108/zsj.24.547

Thompson, J.D., Higgins, D.G. & Gibson, T.J. (1994) ClustalW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice Thompson, Julie D.; Higgins, Desmond G.; Gibson, Toby Journal of Nucleic Acids Research, 22, 4673−4680.
https://doi.org/10.1093/nar/22.22.4673

Tschudi, J.J. von (1838) Classification der Batrachier mit Berücksichtigung der fossilen Thiere dieser Abtheilung der Reptilien. Petitpierre, Neuchâtel, 99 pp.
https://doi.org/10.5962/bhl.title.59545

Vogt, T. (1911) Beitrag zur Amphibien-fauna der Insel Formosa. Sitzungsberichte der Gesellschaft Naturforschender Freunde zu Berlin, 1911, 179–184.

Wijayathilaka, N. & Meegaskumbura, M. (2016) An acoustic analysis of the genus Microhyla (Anura: Microhylidae) of Sri Lanka. PloS ONE, 11, e0159003.
https://doi.org/10.1371/journal.pone.0159003

Wijayathilaka, N., Garg, S., Senevirathne, G., Karunarathna, N., Biju, S.D. & Meegaskumbura, M. (2016) A new species of Microhyla (Anura: Microhylidae) from Sri Lanka: an integrative taxonomic approach. Zootaxa, 4066 (3), 331–342.
https://doi.org/10.11646/zootaxa.4066.3.9

Zug, G. & Mitchell, J. (1995) Amphibians and reptiles of the Royal Chitwan National Park, Nepal. Asiatic Herpetological Research, 6, 172–180.




DOI: http://dx.doi.org/10.11646/zootaxa.4254.2.4

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