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A new lizard species of the Phymaturus patagonicus group (Squamata: Liolaemini) from northern Patagonia, Neuquén, Argentina

ANDREA GoNZÁLEZ MARÍN, CRISTIAN hERNÁN FULVIO PÉREZ, IGNACIO MINOLI, Mariana Morando, Luciano Javier Avila

Abstract


The integrative taxonomy framework allows developing robust hypotheses of species limits based on the integration of results from different data sets and analytical methods. In this work, we test a candidate species hypothesis previously suggested based on molecular data, with geometric and traditional morphometrics analyses (multivariate and univariate). This new lizard species is part of the Phymaturus patagonicus group (payuniae clade) that is distributed in Neuquén and Mendoza provinces (Argentina). Our results showed that Phymaturus rahuensis sp. nov. differs from the other species of the payuniae clade by a higher number of midbody scales, and fewer supralabials scales, finger lamellae and toe lamellae. Also, its multidimensional spaces, both based on continuous lineal variables and geometric morphometrics (shape) characters, do not overlap with those of the other species in this clade. The results of the morphometric and geometric morphometric analyses presented here, coupled with previously published molecular data, represent three independent lines of evidence that support the diagnosis of this new taxon.

 


Keywords


Reptilia, Argentina, Iguanidae sensu lato, Liolaemini, patagonicus group, Phymaturus rahuensis sp. nov., Neuquén

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References


Abdala, C.S. & Lobo, F. (2006) Nueva especie para el grupo de Liolaemus darwinii (Iguania: Liolaemidae) del noroeste de Argentina. Cuadernos de Herpetología, 19 (2), 3–18.

Abdi, H., Williams, L.J. & Valentin, D. (2013) Multiple factor analysis: principal component analysis for multitable and multiblock data sets. Wiley Interdisciplinary Reviews: Computational Statistics, 5 (2), 149–179.
http://dx.doi.org/10.1002/wics.1246

Adams, D.C., Rohlf, F.J. & Slice, D.E. (2004) Geometric morphometrics: ten years of progress following the ‘revolution’. Italian Journal Zoology, 71, 5–16.
http://dx.doi.org/10.1080/11250000409356545

Aguilar, C., Wood, P. Jr., Cusi, J., Guzmán, A., Huari, F., Lundberg, M., Mortensen, E., Ramírez, C., Robles, D., Suárez, J., Ticona, A., Vargas, V., Venegas, P. & Sites, J. (2013) Integrative taxonomy and preliminary assessment of species limits in the Liolaemus walkeri complex (Squamata, Liolaemidae) with descriptions of three new species from Peru. ZooKeys, 364, 47–91.
http://dx.doi.org/10.3897/zookeys.364.6109

Avila, L.J. (2003) A new species of Liolaemus (Squamata: Liolaemidae) from northeastern Argentina and southern Paraguay. Herpetologica, 59 (2), 283–292.
http://dx.doi.org/10.1655/0018-0831(2003)059[0283:ANSOLS]2.0.CO;2

Avila, L.J., Pérez, C.H.F., Perez, D.R. & Morando, M. (2011) Two new mountain lizard species of the Phymaturus genus (Squamata: Iguania) from northwestern Patagonia, Argentina. Zootaxa, 2924, 1–21.

Avila, L.J., Pérez, C.H.F., Minoli, I. & Morando, M. (2014) A new lizard of the Phymaturus genus (Squamata: Liolaemidae) from Sierra Grande, northeastern Patagonia, Argentina. Zootaxa, 3793 (1), 99–118.
http://dx.doi.org/10.11646/zootaxa.3793.1.4

Breitman, M.F., Parra, M. & Pérez, C.H.F. (2011a) Two new species of lizards from the Liolaemus lineomaculatus section (Squamata: Iguania: Liolaemidae) from southern Patagonia. Zootaxa, 3120, 1–28.

Breitman, M.F., Pérez, C.H.F., Parra, M., Morando, M., Sites, J. & Avila, L.J. (2011b) New species of lizard from the magellanicus clade of the Liolaemus lineomaculatus section (Squamata: Iguania: Liolaemidae) from southern Patagonia. Zootaxa, 3123, 32–48.

Bookstein, F.L. (1991) Morphometric tools for landmark data. Geometry and biology. New York: Cambridge University Press.

Cei, J.M. (1986) Reptiles del centro, centro-oeste y sur de la Argentina. Herpetofauna de las zonas áridas y semiáridas. Monograph IV. Museo Regionale di Scienze Naturali Torino, 1–527.

Cei, J.M. (1993) Reptiles del Noroeste, Nordeste y Este de la Argentina. Herpetofauna de las Selvas subtropicales, Puna y Pampas. Monograph XIV. Museo Regionale di Scienze Naturali Torino, 1–949.

Cei, J.M. & Castro, L.P. (1973) Taxonomic and serological researches on the Phymaturus patagonicus complex. Journal of Herpetology, 7, 237–247.
http://dx.doi.org/10.2307/1563009

Cei, J.M. & Roig, V.G. (1975) A new lizard from the Sierra del Nevado Mountains, Central Argentina. Journal of Herpetology, 9, 256.
http://dx.doi.org/10.2307/1563055

Claude, J. (2008) Morphometrics with R. Morphometrics with R XVIII. Springer, New York, 318 pp.

Conover, W.J. (1999) Practical Nonparameteric Statistics. Practical Nonparameteric Statistics 3 rd Edition. Wiley, New York, 592 pp.

Dayrat, B. (2005) Towards integrative taxonomy. Biological Journal of the Linnean Society, 85 (3), 407–415.
http://dx.doi.org/10.1111/j.1095-8312.2005.00503.x

De Queiroz, K. (1998) The general lineage concept of species, species criteria, and the process of speciation. In: Howard, D. J. & Berlocher, S.H. (Eds.), Endless Forms: Species and Speciation Oxford University Press, Oxford, USA, pp. 57–75.

De Queiroz, K. (2007) Species concepts and species delimitation. Systematic Biology, 56 (6), 879–886.
http://dx.doi.org/10.1080/10635150701701083

Etheridge, R. (1995) Redescription of Ctenoblepharys adspersa Tschudi, 1845, and the taxonomy of Liolaeminae (Reptilia: Squamata: Tropiduridae). American Museum of Natural History Novitates, 3142, 1–34.

Florio, A.M., Ingram, C.M., Rakotondravony, H.A., Louis, E.E. & Raxworthy, C.J. (2012) Detecting cryptic speciation in the widespread and morphologically conservative carpet chameleon (Furcifer lateralis) of Madagascar. Journal of Evolutionary Biology 25 (7), 1399–1414.
http://dx.doi.org/10.1111/j.1420-9101.2012.02528.x

Frost, D. (1992) Phylogenetic analysis and taxonomy of the Tropidurus group of lizards (Iguania, Tropiduridae). American Museum Novitates, 3033, 1–68.

García Morabito, E. (2010) Tectónica y estructura del retroarco andino entre los 38°15' y los 40°S. Tesis Doctoral Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires. Avaliable from: http://digital.bl.fcen.uba.ar/Download/Tesis/Tesis_4699_GarciaMorabito.pdf (Accessed 8 Jun. 2016)

Husson, F., Josse, J., Lê, J. & Mazet, J. (2013) FactoMineR: Multivariate Exploratory Data Analysis and Data Mining with R. R package version 1.21.

Kaliontzopoulou, A. (2011) Geometric morphometrics in herpetology: modern tools for enhancing the study of morphological variation in amphibians and reptiles. Basic y Applied Herpetology, 25, 5–32.
http://dx.doi.org/10.11160/bah.11016

Kaliontzopoulou, A., Carretero, M.A. & Llorente, G.A. (2012) Morphology of the Podarcis wall lizards (Squamata: Lacertidae) from the Iberian Peninsula and North Africa: patterns of variation in a putative cryptic species complex. Zoological Journal of the Linnean Society, 164, 173–193.
http://dx.doi.org/10.1111/j.1096-3642.2011.00760.x

Klingenberg, C.P. (2011) MorphoJ: an integrated software package for geometric morphometrics. Molecular Ecology Resources, 11, 353–357.
http://dx.doi.org/10.1111/j.1755-0998.2010.02924.x

Köhler, G. (2012) Color Catalogue for Field Biologists. Herpeton Eds., Offenbach, Germany.

Lê, S., Josse, J. & Husson, F. (2008) FactoMineR: An R package for multivariate analysis. Journal of statistical software, 25, 1–18.
http://dx.doi.org/10.18637/jss.v025.i01

Lobo, F. & Quinteros, S. (2005a) A morphology-based phylogeny of Phymaturus (Iguania: Liolaemidae) with the description of four new species from Argentina. Papeis Avulsos de Zoología, 45, 143–177.
http://dx.doi.org/10.1590/S0031-10492005001300001

Lobo, F. & Quinteros, S. (2005b) Taxonomic studies of the genus Phymaturus (Iguania: Liolaemidae): Redescription of Phymaturus patagonicus Koslowsky 1898, and revalidation and redescription of Phymaturus spurcus Barbour 1921. Journal of Herpetology, 39, 535–540.
http://dx.doi.org/10.1670/170-04A.1

Lobo, F., Abdala, C.S. & Valdecantos, S. (2010) Taxonomic studies of the genus Phymaturus (Iguania: Liolaemidae): Description of four new species. South American Journal of Herpetology, 5, 102–126.
http://dx.doi.org/10.2994/057.005.0205

Lobo, F., Cruz, F.B. & Abdala, C.S. (2012a) Multiple lines of evidence show that Phymaturus agilis Scolaro, Ibargüengoytía & Pincheira-Donoso, 2008 is a junior synonym of Phymaturus spectabilis Lobo & Quinteros, 2005. Cuadernos de herpetología, 26, 21–27.

Lobo, F., Abdala, C.S. & Valdecantos, S. (2012b) Morphological diversity and phylogenetic relationships within a South-American clade of iguanian lizards (Liolaemidae: Phymaturus). Zootaxa, 3315, 1–41.

Lobo, F. & Nenda, S.J. (2015) Discovery of two new species of Phymaturus (Iguania: Liolaemidae) from Patagonia, Argentina, and occurrence of melanism in the patagonicus group. Cuadernos de Herpetología, 29 (1), 5–25.

Marshall, J.C. & Sites, J.W. Jr. (2003) A summary of some contemporary methods used to delimit species boundaries. Boletín de la Sociedad Herpetológica Mexicana, 11 (1), 1–8.

Minoli, I., Morando, M. & Avila, L.J. (2014) Integrative taxonomy in the Liolaemus fitzingerii complex (Squamata: Liolaemini) based on morphological analyses and niche modeling. Zootaxa, 3856 (4), 501–528.
http://dx.doi.org/10.11646/zootaxa.3856.4.3

Morando, M., Avila, L.J. Pérez, C.H.F. Hawkins, M. & Sites, J.W. (2013) Molecular phylogeny of Phymaturus (Squamata, Liolaemini): too many lineages and few names. Molecular Phylogenetics and Evolution, 66, 694–714.
http://dx.doi.org/10.1016/j.ympev.2012.10.019

Ocampo, M., Aguilar-Kirigin, Á. & Quinteros, S. (2012) A New Species of Liolaemus (Iguania: Liolaemidae) of the alticolor group from La Paz, Bolivia. Herpetologica, 68 (3), 410–417.
http://dx.doi.org/10.1655/HERPETOLOGICA-D-12-00001.1

Padial, J.M. & De La Riva, I. (2007) Integrative taxonomists should use and produce DNA barcodes. Philosophical Transactions of the Royal Society of London, 68, 67–68.

Padial, J.M., Miralles, A., De la Riva, I. & Vences, M. (2010) The integrative future of taxonomy. Frontiers in Zoology, 7 (16), 1–14.
http://dx.doi.org/10.1186/1742-9994-7-16

Rabassa, J., Coronato, A., Ponce, J.F., Schlieder, G. & Martinez, O. (2011) Depósitos glacigenicos (Cenozoico Tardio – Cuaternario) y geoformas asociadas. Relatorio XVIII Congreso Geológico Argentino, Neuquén.

Riccardi, A.C. (1993) Formación Los Molles. In: Riccardi, A.C. & Damborenea, S.E. (Eds.), Léxico estratigráfico de la Argentina. Volumen 9, Jurásico. Asociación Geológica Argentina, Serie B, Didáctica y Complementaria 21, Buenos Aires, Argentina, pp. 239–243.

Roig, A.F. (1998) La vegetación de la Patagonia. In: Correa, M.N. (Ed.), Flora Patagónica. INTA, Buenos Aires, Argentina, pp. 48–174.

R Core Team (2014) R: A language and environment for statistical computing. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. Available from: http://www.R-project.org/ (Accessed 8 Jun. 2016)

Smith, H.M. (1946) Handbook of lizards. Comstock Publishing Company, Ithaca, New York, U.S.A, 557 pp.

Rohlf, F.J. & Slice, D.E. (1990) Extensions of the Procrustes method for the optimal superimposition of landmarks. Systematic Zoology, 39, 40–59.
http://dx.doi.org/10.2307/2992207

Rohlf, F.J. (2010a) tpsDig version 2.16. Department of Ecology and Evolution, State University of New York at Stony Brook.

Rohlf, F.J. (2010b) Relative warps version 1.49. Department of Ecology and Evolution, State University of New York at Stony Brook.

Rohlf, F.J. (2012) tpsUtility program version 1.52. Department of Ecology and Evolution, State University of New York at Stony Brook.

Scolaro, J.A. & Tapari, F.O. (2009) Una nueva especie del género Phymaturus del ‘grupo patagonicus’ en los afloramientos rocosos del sudoeste de la provincia de Río Negro, Patagonia Argentina (Reptilia: Iguania: Liolaemidae). Naturalia patagónica, 5 (1), 80–93.

Scolaro, J.A., Jara, M. & Pincheira-Donoso, D. (2013) The sexual signals of speciation? A new sexually dimorphic Phymaturus species of the patagonicus clade from Patagonia Argentina. Zootaxa, 3722 (3), 317–332.
http://dx.doi.org/10.11646/zootaxa.3722.3.2

Sheets, H.D. (2005) MakeFan, a tool for drawing alignment “fans” at equal angular spacing. Available from: http://www3.canisius.edu/~sheets/morphsoft.html (Accessed 8 Jun. 2016)

Simmons, J.E. (2002) Herpetological Collecting and Collections Managements. Society for the Study of Amphibians and Reptiles, Herpetological Circular, USA, 159 pp.

Sites, J.W. & Marshall, J.C. (2003) Delimiting species: a Renaissance issue in systematic biology. TRENDS in Ecology and Evolution, 18, 462–70.
http://dx.doi.org/10.1016/S0169-5347(03)00184-8

Sites, J.W. & Marshall, J.C. (2004) Operational criteria for delimiting species. Annual Review of Ecology, Evolution and Systematics, 35, 199–227.
http://dx.doi.org/10.1146/annurev.ecolsys.35.112202.130128

Wiens, J.J. & Servedio, M.R. (2000) Species delimitation in systematics: inferring diagnostic differences between species. Proceedings Royal Society of London, B, 267, 631–636.
http://dx.doi.org/10.1098/rspb.2000.1049

Zapata, F. & Jiménez, I. (2012) Species delimitation: inferring gaps in morphology across geography. Systematic Biology, 61, 179–194.
http://dx.doi.org/10.1093/sysbio/syr084

Zelditch, M.L., Swiderski, D.L., Sheets, H.D. & Fink, W.L. (2004) Geometric Morphometrics for biologists: a primer. Elsevier Academic Press, London, 443 pp.




DOI: http://dx.doi.org/10.11646/zootaxa.4121.4.3

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