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Type: Article
Published: 2008-12-03
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A new Gonatocerus (Hymenoptera: Mymaridae) from Argentina, with taxonomic notes and molecular data on the G. tuberculifemur species complex

Entomology Research Museum, Department of Entomology, University of California, Riverside, California, 92521, USA
USDA, ARS South American Biological Control Laboratory, 3130 Buenos Aires Place, Washington, D.C. 20521–3130, USA
Beneficial Insects Research Unit, Kika de la Garza Subtropical Agricultural Research Center, USDA-ARS, 2413 E. Highway 83, Weslaco, Texas, 78596, USA
CONICET-PROIMI, Avenida Belgrano y Pasaje Caseros, T4001MVB, San Miguel de Tucumán, Tucumán, Argentina
Hymenoptera Mymaridae Gonatocerus taxonomy Cicadellidae Proconiini egg parasitoid Argentina

Abstract

Gonatocerus deleoni Triapitsyn, Logarzo & Virla sp. n., reared from sentinel eggs of Tapajosa rubromarginata (Signoret) (Cicadellidae: Cicadellinae: Proconiini) on citrus plants, a new member of the ater species group of Gonatocerus Nees (Mymaridae), is described from the state of Mendoza, Argentina. Taxonomic notes and host association data are provided to help differentiate this new species from the morphologically similar but genetically distinct taxon, G. tuberculifemur (Ogloblin). The female of the latter is redescribed and the male is newly described. Gonatocerus deleoni, G. tuberculifemur, and three forms (different molecular clades) comprise the G. tuberculifemur complex. These forms are identified but not formally described because of lack of morphologically distinguishing features. The taxonomic conclusions are supported by molecular data, and by results of reciprocal cross-breeding experiments between most of them.

References

  1. Chang, S.-C., Hu, N.-T., Hsin, C.-Y. & Sun, C.-N. (2001) Characterization of differences between two Trichogramma wasps by molecular markers. Biological Control, 21, 75–78.

    de León, J.H., Jones, W.A. & Morgan, D.J.W. (2004) Molecular distinction between populations of Gonatocerus morrilli, egg parasitoids of the glassy-winged sharpshooter Homalodisca coagulata, from Texas and California: Do cryptic species exist? Journal of Insect Science, 4(39), 7 pp. Available online at http://insectscience.org/4.39.

    de León, J.H., Jones, W.A., Sétamou, M. & Morgan, D.J.W. (2006) Genetic and hybridization evidence confirms that a geographic population of Gonatocerus morrilli (Hymenoptera: Mymaridae) from California is a new species: Egg parasitoids of the glassy-winged sharpshooter Homalodisca coagulata (Homoptera: Cicadellidae). Biological Control, 38, 282–293.

    de León, J.H., Logarzo, G.A. & Triapitsyn, S.V. (2006a) Genetic characterization of Gonatocerus tuberculifemur from South America uncovers divergent clades: Prospective egg parasitoid candidate agent for the glassy-winged sharpshooter in California. In: Proceedings of the 2006 Pierce’s Disease Research Symposium, November 27–29, 2006, The Westin Horton Plaza Hotel, San Diego, California, organized by California Department of Food and Agriculture (Esser, T., Chief Ed., compiled by Tariq, M.A., Medeiros, R., Mochel, M. & Veling, S.). Copeland Printing, Sacramento, California, pp. 40–43. Available online at http://www.cdfa.ca.gov/pdcp/Research_Symposium_Index.html.

    de León, J.H., Logarzo, G.A. & Triapitsyn, S.V. (2006b) Preliminary evidence from reproductive compatibility studies suggests that Gonatocerus tuberculifemur exists as a cryptic species complex, or a new species is identified: Development and utility of molecular diagnostic markers. In: Proceedings of the 2006 Pierce’s Disease Research Symposium, November 27–29, 2006, The Westin Horton Plaza Hotel, San Diego, California, organized by California Department of Food and Agriculture (Esser, T., Chief Ed., compiled by Tariq, M.A., Medeiros, R., Mochel, M. & Veling, S.). Copeland Printing, Sacramento, California, pp. 44–47. Available online at http://www.cdfa.ca.gov/pdcp/Research_Symposium_Index.html.

    de León, J.H., Logarzo, G.A. & Triapitsyn, S.V. (2006c) ISSR-PCR DNA fingerprinting uncovers distinct banding patterns in Gonatocerus species 3 (G. sp. 3) individuals emerging from different host tribes: A prospective egg parasitoid candidate agent for the glassy-winged sharpshooter in California. In: Proceedings of the 2006 Pierce’s Disease Research Symposium, November 27–29, 2006, The Westin Horton Plaza Hotel, San Diego, California, organized by California Department of Food and Agriculture (Esser, T., Chief Ed., compiled by Tariq, M.A., Medeiros, R., Mochel, M. & Veling, S.). Copeland Printing, Sacramento, California, pp. 48–51. Available online at http://www.cdfa.ca.gov/pdcp/Research_Symposium_Index.html.

    de León, J.H., Logarzo, G.A. & Triapitsyn, S.V. (2006d) The utility of the internal transcribed spacer region 2 (ITS2) in confirming species boundaries in the genus Gonatocerus: Comparison to the cytochrome oxidase subunit I (COI) gene and taxonomic data: Molecular key based on ITS2 sizes. In: Proceedings of the 2006 Pierce’s Disease Research Symposium, November 27–29, 2006, The Westin Horton Plaza Hotel, San Diego, California, organized by California Department of Food and Agriculture (Esser, T., Chief Ed., compiled by Tariq, M.A., Medeiros, R., Mochel, M. & Veling, S.). Copeland Printing, Sacramento, California, pp. 56–59. Available online at http://www.cdfa.ca.gov/pdcp/Research_Symposium_Index.html.

    de León, J.H., Logarzo, G.A. & Triapitsyn, S.V. (2007) Progress on resolving the Gonatocerus tuberculifemur complex: Neither COI nor ITS2 sequence data alone can discriminate all the species within the complex, whereas, ISSR-PCR DNA fingerprinting can. In: Proceedings of the 2007 Pierce’s Disease Research Symposium, December 12–14, 2007, The Westin Horton Plaza Hotel, San Diego, California, organized by California Department of Food and Agriculture (Esser, T., Chief Ed., compiled by Blincoe, P., West, D., Mochel, M. & Veling, S.). PIP Printing and Document Services, Sacramento, California, pp. 73–76. Available online at http://www.cdfa.ca.gov/pdcp/Research_Symposium_Index.html.

    de León, J.H., Logarzo, G.A. & Triapitsyn, S.V. (2008) Molecular characterization of Gonatocerus tuberculifemur (Ogloblin) (Hymenoptera: Mymaridae), a prospective Homalodisca vitripennis (Germar) (Hemiptera: Cicadellidae) biological control candidate agent from South America: divergent clades. Bulletin of Entomological Research, 98, 97–108.

    de León, J.H., Triapitsyn, S.V., Matteucig, G. & Viggiani, G. (2008) Molecular and morphometric analyses of Anagrus erythroneurae S. Trjapitzin & Chiappini and A. ustulatus Haliday (Hymenoptera: Mymaridae). Bollettino del Laboratorio di Entomologia agraria «Filippo Silvestri», Portici, submitted (in review).

    De Santis, L. (1967) Catálogo de los hymenópteros argentinos de la serie Parasítica, incluyendo Bethyloidea. Comisión de Investigación Científica, Gobernación de la Provincia de Buenos Aires, La Plata, 337 pp.

    Felsenstein, J. (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 39, 783–791.

    Gibson, G.A.P. (1997) Chapter 2. Morphology and terminology. In: Gibson, G.A.P., Huber, J.T. & Woolley, J.B. (Ed.), Annotated keys to the genera of Nearctic Chalcidoidea (Hymenoptera). NRC Research Press, Ottawa, Ontario, Canada, pp. 16–44.

    Hoddle, M. & Irvin, N. (2007) Should neoclassical biological control agents from Argentina be released in California for control of the glassy-winged sharpshooter? In: Proceedings of the 2007 Pierce’s Disease Research Symposium, December 12–14, 2007, The Westin Horton Plaza Hotel, San Diego, California, organized by California Department of Food and Agriculture (Esser, T., Chief Ed., compiled by Blincoe, P., West, D., Mochel, M. & Veling, S.). PIP

    Printing and Document Services, Sacramento, California, pp. 89–92. Available online at http://www.cdfa.ca.gov/pdcp/Research_Symposium_Index.html.

    Hoddle, M.S. & Triapitsyn, S.V. (2005) Maintaining and evaluating quarantine cultures of Gonatocerus spp., promising egg parasitoids from Argentina and Mexico, for the classical biological control of the glassy-winged sharpshooter in California. In: Proceedings of the 2005 Pierce’s Disease Research Symposium, December 5–7, 2005, San Diego Marriott Hotel & Marina, San Diego, California, organized by California Department of Food and Agriculture (compiled by Tariq, M.A., Blincoe, P., Mochel, M., Oswalt, S. & Esser, T.). Copeland Printing, Sacramento, California, pp. 341–342. Available online at http://www.cdfa.ca.gov/pdcp/Research_Symposium_Index.html.

    Hoy, M.A., Ayyamperumal, J., Morakete, R., Lo, M.K.C. & Nguyen, R. (2000) Genomic analyses of two populations of Ageniaspis citricola (Hymenoptera: Encyrtidae) suggest that a cryptic species may exist. Biological Control, 17, 1–10.

    Huber, J. T. (1988) The species groups of Gonatocerus Nees in North America with a revision of the sulphuripes and ater groups (Hymenoptera: Mymaridae). Memoirs of the Entomological Society of Canada, 141, 1–109.

    Jones, W.A. (2001) Classical biological control of the glassy-winged sharpshooter. In: Proceedings of the Pierce’s Disease Research Symposium, December 5–7, 2001, Coronado Island Marriott Resort, San Diego, California (compiled by Tariq, M.A., Oswalt, S. & Esser, T.). California Department of Food and Agriculture, Copeland Printing, Sacramento, California, pp. 50–51. Available online at http://www.cdfa.ca.gov/pdcp/Research_Symposium_Index.html.

    Jones, W.A., Logarzo, G.A., Triapitsyn, S.V., Casas, M., Virla, E.G. & Purcell, A.H. (2005) Biology and host range of 2 South American egg parasitoids (Hymenoptera: Mymaridae), possible biocontrol agents for Glassy-Winged Sharpshooter, Homalodisca coagulata (Say) (Hemiptera: Cicadellidae; Proconiini). In: Submitted Papers T1–T21, 12th International Auchenorrhyncha Congress and 6th International Workshop on Leafhoppers and Planthoppers of Economic Significance. University of California, Berkeley, 8–12 August 2005, Submitted Papers T7–T8. Available online at http://nature.berkeley.edu/hoppercongress/.

    Jones, W.A., Logarzo, G.A., Virla, E.G. & Luft, E. (2005) Environmental risk assessment of egg parasitoids from South America: Nontarget field and laboratory host range in Argentina and the United States. In: Proceedings of the 2005 Pierce’s Disease Research Symposium, December 5–7, 2005, San Diego Marriott Hotel & Marina, San Diego, California, organized by California Department of Food and Agriculture (compiled by Tariq, M.A., Blincoe, P.,

    Mochel, M., Oswalt, S. & Esser, T.). Copeland Printing, Sacramento, California, pp. 343–344. Available online at http://www.cdfa.ca.gov/pdcp/Research_Symposium_Index.html.

    Logarzo, G.A., de León, J.H., Triapitsyn, S.V., González, R.H. & Virla, E.G. (2006) First report of a proconiine sharpshooter, Anacuerna centrolinea (Hemiptera: Cicadellidae), in Chile, with notes on its biology, host plants, and egg parasitoids. Annals of the Entomological Society of America, 99(5), 879–883.

    Logarzo, G.A., Virla, E. & Jones, W.A. (2005) Egg parasitoids from Argentina, potential candidates for the biological control of glassy-winged sharpshooter in the United States. In: Second International Symposium on Biological Control of Arthropods, Davos, Switzerland, September 12–16, 2005 (Hoddle, M.S., Compiler). USDA Forest Service Publication FHTET–2005–08, Volume III, pp. 115–116. Available online at http://www.bugwood.org/arthropod2005/vol3/index.html.

    Logarzo, G.A., Virla, E.G., Luft Albarracin, E., Triapitsyn, S.V., Jones, W.A. & de León, J.H. (2008) Native host range of Gonatocerus sp. near tuberculifemur “Clade 1” (Hymenoptera: Mymaridae) in Argentina, a candidate egg parasitoid agent for the neoclassical biological control of the glassy-winged sharpshooter, Homalodisca vitripennis (Hemiptera: Cicadellidae) in California, USA. Biological Control, submitted (in review).

    Ogloblin, A.A. (1957 [1956]) Las especies argentinas del género Lymaenon (Halid.) Walk. (Mymaridae, Hymenoptera). Revista de la Sociedad Entomológica Argentina, 19(3–4), 33–43.

    O'Neill, S.L., Giordano, R., Colbert, A.M.E., Karr, T.L. & Robertson, H.M. (1992) 16S rRNA phylogenetic analysis of the bacterial endosymbionts associated with cytoplasmic incompatibility in insects. Proceedings of the National Academy of Sciences of the United States of America, 89, 2699-2702.

    Pilkington, L.J., Irvin, N.A., Boyd, E.A., Hoddle, M.S., Triapitsyn, S.V., Carey, B.G., Jones, W.A. & Morgan, D.J.W. (2005) Introduced parasitic wasps could control glassy-winged sharpshooter. California Agriculture, 59(4), 223–228.

    Porter, C.H. & Collins, F.H. (1991) Species-diagnostic difference in a ribosomal DNA internal transcribed spacer from the sibling species Anopheles freeborni and Anopheles hermsi (Diptera: Culicidae). American Journal of Tropical Medicine and Hygiene, 45, 271–279.

    Saitou, N. & Nei, M. (1987) The neighbour-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4, 406–425.

    Simon, C., Frati, F., Bechenbach, A., Crespi, B., Liu, H. & Flook, P. (1994) Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Annals of the Entomological Society of America, 87, 651–701.

    Swofford, D.L. (2002) PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods). Version 4. Sinauer Associates, Sunderland, Massachusetts.

    Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. & Higgins, D.G. (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25, 4876–4882.

    Triapitsyn, S. V. (2002a) Species-level taxonomy of Mymaridae (Hymenoptera): current status and implications for biological control of leafhoppers of economic importance. In: Melika, G. & Thuróczy, C. (Ed.), Parasitic wasps: Evolution, systematics, biodiversity and biological control. Agroinform, Kiadó & Nyomda Kft., Budapest, pp. 89–94.

    Triapitsyn, S. V. (2002b) Taxonomy and host associations of Gonatocerus spp. (Mymaridae) – egg parasitoids of proconiine leafhoppers. Egg Parasitoid News, 14, 10.

    Triapitsyn, S.V. (2006a) A key to the Mymaridae (Hymenoptera) egg parasitoids of proconiine sharpshooters (Hemiptera: Cicadellidae) in the Nearctic region, with description of two new species of Gonatocerus. Zootaxa, 1203, 1–38.

    Triapitsyn, S.V. (2006b) Identify the species of Mymaridae reared in Argentina and Mexico for potential introduction to California against the glassy-winged sharpshooter and prepare and submit for publication a pictorial, annotated key to the ater-group species of Gonatocerus – egg parasitoids of the proconiine sharpshooters (Hemiptera: Cicadellidae: Proconiini) in the Neotropical region. In: Proceedings of the 2006 Pierce’s Disease Research Symposium, November 27–29, 2006, The Westin Horton Plaza Hotel, San Diego, California, organized by California Department of Food and Agriculture (Esser, T., Chief Ed., compiled by Tariq, M.A., Medeiros, R., Mochel, M. & Veling, S.). Copeland Printing, Sacramento, California, pp. 111–113. Available online at http://www.cdfa.ca.gov/pdcp/Research_Symposium_Index.html.

    Triapitsyn, S.V. (2007) Identifying the species of Mymaridae reared in Argentina and Mexico for potential introduction to California against the glassy-winged sharpshooter and prepare and submitting for publication a pictorial, annotated key to the ater-group species of Gonatocerus – egg parasitoids of the proconiine sharpshooters (Hemiptera: Cicadellidae: Proconiini) in the Neotropical region. In: Proceedings of the 2007 Pierce’s Disease Research Symposium, December 12–14, 2007, The Westin Horton Plaza Hotel, San Diego, California, organized by California Department of Food and Agriculture (Esser, T., Chief Ed., compiled by Blincoe, P., West, D., Mochel, M. & Veling, S.). PIP Printing and Document Services, Sacramento, California, pp. 58–61. Available online at http://www.cdfa.ca.gov/pdcp/Research_Symposium_Index.html.

    Triapitsyn, S.V., Logarzo, G.A., Virla, E.G. & de León, J.H. (2007) A new species of Gonatocerus (Hymenoptera: Mymaridae) from Argentina, an egg parasitoid of Tapajosa rubromarginata (Hemiptera: Cicadellidae). Zootaxa, 1619, 61–68.

    Triapitsyn, S.V., Vickerman, D.B., Heraty, J.M. & Logarzo, G.A. (2006) A new species of Gonatocerus (Hymenoptera: Mymaridae) parasitic on proconiine sharpshooters (Hemiptera: Cicadellidae) in the New World. Zootaxa, 1158, 55–67.

    Virla, E.G., Logarzo, G.A., Jones, W.A. & Triapitsyn, S. (2005) Biology of Gonatocerus tuberculifemur (Hymenoptera: Mymaridae), an egg parasitoid of the sharpshooter, Tapajosa rubromarginata (Hemiptera: Cicadellidae). Florida Entomologist, 88(1), 67–71.

    Virla, E.G., Logarzo, G.A., Paradell, S.L. & Triapitsyn, S.V. (2008) Bionomics of Oncometopia tucumana (Hemiptera: Cicadellidae), a sharpshooter from Argentina, with notes on its distribution, host plants, and egg parasitoids. Florida Entomologist, 91(1), 55-62.

    Virla, E., Logarzo, G., Triapitsyn, S. & Jones, W. (2003) Biología de Gonatocerus tuberculifemur (Hymenoptera:

    Mymaridae), candidato para el control de Homalodisca coagulata (Hemiptera: Cicadellidae) en EEUU. In: Agroecología. VIII Jornadas de Ciencias Naturales del Litoral. I Jornada de Ciencias Naturales del NOA. Argentina, 22–26 September 2003, p. 102.

    Werren, J.H. (1997) Biology of Wolbachia. Annual Review of Entomology, 42, 587–609.

    White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Shinsky, J.J. & White, T.J. (Ed.), PCR protocols: a guide to methods and applications. Academic Press, San Diego, pp. 315–322.

    Yoshimoto, C.M. (1990) A review of the genera of New World Mymaridae (Hymenoptera: Chalcidoidea). Flora & Fauna Handbook No. 7, Sandhill Crane Press, Inc., Gainesville, Florida, v–ix + 166 pp.

    Zietkiewicz, E., Rafalski, A. & Labuda, D. (1994) Genomic fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics, 20, 176–183.