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Issue: Vol. 3569 No. 1: 30 Nov. 2012
Type: Articles
Published: 2012-11-30
DOI: 10.11646/zootaxa.3569.1.2
Page range: 25–40
Abstract views: 51
PDF downloaded: 1

Heterorhabditis beicherriana n. sp. (Nematoda: Heterorhabditidae), a new entomopathogenic nematode from the Shunyi district of Beijing, China

Laboratory of Entomology and Nematology, College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, China
Laboratory of Entomology and Nematology, College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, China
Laboratory of Entomopathogenic Nematodes, Institute of Entomology, Biology Centre, Czech Academy of Sciences, Branišovská 31, 370 05, České Budějovice, The Czech Republic
Laboratory of Entomopathogenic Nematodes, Institute of Entomology, Biology Centre, Czech Academy of Sciences, Branišovská 31, 370 05, České Budějovice, The Czech Republic
Laboratory of Entomopathogenic Nematodes, Institute of Entomology, Biology Centre, Czech Academy of Sciences, Branišovská 31, 370 05, České Budějovice, The Czech Republic
Nematoda new species description morphology taxonomy systematics phylogeny SEM

Abstract

A new heterorhabditid nematode was recovered in orchards in the Beijing area. Morphological and molecular dataconfirmed this nematode as a new species, which belongs to the bacteriophora-group with sister taxa Heterorhabditisbacteriophora and H. georgiana. The nematode was named H. beicherriana n. sp. and is characterized by males,hermaphrodites, females and infective juveniles. In males the peloderan bursa is characterized by the formula 1 2 3 3,spicule length 45 (41–51)μm. Male body length is substantially higher than that of H. bacteriophora and H. georgiana(1028μm vs 820 and 838μm, respectively). In hermaphrodites there are six labial and six cephalic papillae; the vulvalpattern is smooth, rounded or slightly elliptical. Tail is conoid with a moderately prominent post-anal swelling slightlylonger than anal body width. In females vulval lips are non-protruding, tail conical without or with a slightly developedpost-anal swelling, about twice as long as anal body width. In infective juveniles, the body is elongate, slender and straightwhen heat-killed, on average 639μm long, which is longer than in H. bacteriophora (558μm) and H. georgiana (598μm),respectively. Heterorhabditis beicherriana n. sp. is further characterized by internal transcribed spacer (ITS) and D2D3rDNA sequences, the most similar species, H. georgiana, being separated by 21 bp across 994 bp of the ITS and 3 across890 bp of the D2D3 region. ITS and D2D3 regions of H. beicherriana evolved five and one autapomorphies, respectively. Phylogenetic analyses placed H. beicherriana n. sp. as a member of the bacteriophora-group.

References

  1. Adams, B.J. (1998) Species concept and the evolutionary paradigm in modern nematology. Journal of Nematology, 30, 1–21.

    Andaló, V., Nguyen, K.B. & Moino JR, A. (2006) Heterorhabditis amazonensis n. sp. (Rhabditida: Heterorhabditidae) from Amazonas, Brazil. Nematology, 8, 853–867.

    Bedding, R.A. & Akhurst, R.J. (1975) A simple technique for the detection of insect parasitic rhabditid in soil. Nematologica, 21, 109.

    Courtney, W.D., Polley, D. & Miller, V.L., (1955) TAF, an improved fixative in nematode technique. Plant Disease Reporter, 39, 570–571.

    Edgington, S., Buddie, A.G., Moore, D., France, A., Merino, L., & Hunt D.J. (2011) Heterorhabditis atacamensis n. sp. (Nematoda: Heterorhabditidae), a new entomopathogenic nematode from the Atacama Desert, Chile. Journal of Helminthology, 85, 381–394.

    Feng-Chia, H., Chiaw-Yen, T., Jui-Tang, T., Yeong-Sheng, T., Menghsiao, M. & Suey-Sheng, K. (2009) Isolation and characterization of the native entomopathogenic nematode Heterorhabditis brevicaudis, and its symbiotic bacteria from Taiwan. Current Microbiology, 58, 564–570.

    Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98.

    Han, R. (1994) Advances in the research of entomopathogenic nematodes Steinernema and Heterorhabditis in China. Entomologia Sinica, 1, 346–364.

    Huelsenbeck, J.P. & Ronquist, F. (2001) MR-BAYES: Bayesian inference of phylogeny. Bioinformatics, 17, 754–755.

    Liu, J. (1994) A new species of the genus Heterorhabditis from China (Rhabditida: Heterorhabditidae). Acta Zootaxonomica Sinica, 19, 268–272.

    Liu, J. & Berry, R.E. (1996) Heterorhabditis marelatus n. sp. (Rhabditida: Heterorhabditidae) from Oregon. Journal of Invertebrate Pathology, 67, 48–54.

    Maddison, W.P. & Maddison D.R. (2002) MacClade, version 4.05. Sunderland, Massachusetts, Sinauer Associates.

    Malan, A.P., Nguyen, K.B., De Waal, J.Y. & Tiedt, L. (2008) Heterorhabditis safricana n. sp. (Rhabditida: Heterorhabditidae), a new entomopathogenic nematode from South Africa. Nematology, 10, 381–396.

    Mráček, Z. & Bednarek, A. (1992) Cuticular structures of J2 and J3 in Heterorhabditidae. Nematologica, 38, 386–390.

    Mráček, Z., Nguyen, K.B., Tailliez, P., Boemare, N. & Shulong, C., 2006. Steinernema sichuanense n. sp. (Rhabditida, Steinernematidae), a new species of entomopathogenic nematode from the provice of Sichuan, east Tibetan Mts., China. Journal of Invertebrate Pathology, 93, 157–169.

    Nguyen, K.B. & Smart, Jr, G.C. (1995) Scanning electron microscope studies of Steinernema glaseri (Nematoda: Steinernematidae). Nematologica, 41, 183–190.

    Nguyen, K.B. & Smart, G.C. (1997) Scanning Electron Microscope Studies of Spicules and Gubernacula of Steinernema Spp. (Nemata: Steinernematidae). Nematologica, 43, 465–480.

    Nguyen, K.B., Shapiro-Ilan, D.I., Stuart, R.J., McCoy, C.W., James, R.R. & Adams, B.J. (2004) Heterorhabditis mexicana n. sp. (Rhabditida: Heterorhabditidae) from Tamaulipas, Mexico, and morphological studies of the bursa of Heterorhabditis spp. Nematology, 6, 231–244.

    Nguyen, K.B., Gozel, U., Koppenhöfer, H.S. & Adams, B.J. (2006) Heterorhabditis floridensis n. sp. (Rhabditida: Heterorhabditidae) from Florida. Zootaxa, 1177, 1–19.

    Nguyen, K.B. & Hunt, D. (2007) Heterorhabditidae: species description. In: Nguyen, K.B. & Hunt, D.J. (Eds), Entomopathogenic nematodes: systematics, phylogeny and bacterial symbionts. Nematology Monographs and Perspectives 5 (Series eds: Hunt, D.J. & Perry, R.N.). Brill, Leiden, The Netherlands, pp. 59–119.

    Nguyen, K.B., Shapiro-Ilan, D. & Mbata, G.N. (2008) Heterorhabditis georgiana n. sp. (Rhabditida: Heterorhabditidae) from Georgia, USA. Nematology, 10, 433–448.

    Nylander, J. A. A. (2004) MrModeltest 2.0. Program distributed by the author. Evolutionary Biology Centre, Uppsala University, Sweden.

    Phan, K.L., Subbotin, S.A., Nguyen, N.C. & Moens, M. (2003) Heterorhabditis baujardi sp. n. (Rhabditida: Heterorhabditidae) from Vietnam and morphometric data for H. indica populations. Nematology, 5, 367–382.

    Plichta, K.L, Joyce S.A., Clarke, D., Waterfield, N. & Stock, S.P. (2009) Heterorhabditis gerrardi n. sp. (Nematoda: Heterorhabditidae): the hidden host of Photorhabdus asymbiotica (Enterobacteriaceae: γ-Proteobacteria). Journal of Helminthology, 83, 309–320.

    Poinar, G.O.Jr. (1976) Description and biology of a new insect parasitic rhabditoid Heterorhabditis bacteriophora n. gen., n. sp. (Rhabditida: Heterorhabditidae n. fam.). Nematologica, 21, 463–470.

    Poinar, G.O. Jr, Jackson, T. & Klein, M. (1987) Heterorhabditis megidis sp. n. (Heterorhabditidae: Rhabditida), parasitic in the Japanese Beetle, Popillia japonica (Scarabaeidae: Coleoptera), in Ohio. Proceedings of the Helminthological Society of Washington, 54, 53–59.

    Poinar, G.O. Jr, (1990) Taxonomy and biology of Steinernematidae and Heterorhabditidae. In Gaugler, R. & Kaya, H.K. (Eds) Entomopathogenic nematodes in biological control. Boca Raton, CRC Press, pp. 23–60.

    Poinar, G.O. Jr, Karunakar, G.K. & David, H. (1992) Heterorhabditis indicus n. sp. (Rhabditida: Nematoda) from India: separation of Heterorhabditis spp. by infective juveniles. Fundamental and Applied Nematology, 15, 467–472.

    Seinhorst, J.W. (1959) A rapid method for the transfer of nematodes from fixative to anhydrous glycerin. Nematologica, 4, 67–69.

    Shamseldean, M.M., Abou El-Sooud, A.B., Abd-Elgawad, M.M. & Saleh, M.M. (1996) Identification of a new Heterorhabditis species from Egypt, Heterorhabditis taysearae n. sp. (Rhabditida: Heterorhabditidae). Egyptian Journal of Biological Control, 6, 129–138.

    Stock, S.P., Griffin, C.T. & Burnell, A.M. (2002) Morphological characterisation of three isolates of Heterorhabditis Poinar, 1976 from the ‘Irish group’ (Nematoda: Rhabditida: Heterorhabditidae) and additional evidence supporting their recognition as a distinct species, H. downesi n. sp. Systematic Parasitology, 51, 95–106.

    Stock, S.P., Rivera-Orduno, B. & Flores-Lara, Y. (2009) Heterorhabditis sonorensis n. sp. (Nematoda: Heterorhabditidae), a natural pathogen of the seasonal cicada Diceroprocta ornea (Walker) (Homoptera: Cicadidae) in the Sonoran desert. Journal of Invertebrate Pathology, 100, 175–184.

    Swofford, D.L. (2002) PAUP* Phylogenetic Analysis Using Parsimony *(and other methods). Sunderland, Massachusetts, Sinauer Associates.

    Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. & Kumar, S. (2011) MEGA5: Molecular evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution, 28, 2731–2739.

    White, G. F. (1929) A method for obtaining infective nematode larvae from cultures. Science, 66, 302–303.

    Vrain, T.C., Wakarchuk, D.A., Levesque, A.C. & Hamilton, R.I. (1992) Intraspecific rDNA restriction fragment length polymorphism in the Xiphinema americanum group. Fundamental and Applied Nematology, 15, 563–73.