Abstract
The frog genus Atelognathus is currently represented by nine species distributed in Argentinean and Chilean Patagonia. It is mainly distributed in Argentina, and there are only three species in Chile (A. ceii, A. grandisonae and A. jeinimenensis). Regarding the morphological relationships among Atelognathus species, Meriggio et al. (2004) suggest that A. jeinimenensis is more related to A. salai than other species. A. salai was described from Laguna Los Gendarmes (Argentina), 90 km air line from the type locality of A. jeinimenensis. This paper presents a morphological analysis and a study of population genetics using mtDNA nucleotide data from Argentinean and Chilean localities to assess the genetic distance between A. salai and A. jenimenensis. We obtained 477 bp-long d-loop sequences from 51 Atelognathus specimens collected in four localities. According to our results, the morphological differences between A. salai and A. jeinimenensis populations are limited to size, and there are no distinctive characteristics that would separate two species, in addition to which a simple geographic pattern of genetic diversity suggests a single species of Atelognathus. Also, the populations from Chile (Cerro Castillo, RN Lago Jeinimeni and Chile Chico) and Argentina (Laguna de Los Gendarmes) have low levels of genetic divergence that may be consistent with glaciations during the Late Pleistocene. We propose Atelognathus jeinimenensis as a junior synonym of A. salai and that the Chilean populations should be assigned to A. salai.References
Aljanabi, S.M. & Martínez, I. (1997) Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques. Nucleic Acids Research, 25, 4692–4693.
Ashworth, A.C., Markgraft, V. & Villagrán, C. (1991) Late Quaternary climatic history of the Chilean Channels based on fossil pollen and beetle analyses, with an analysis of the modern vegetation and pollen rain. Journal of Quaternary Science, 6, 279–291.
Atkinson, D. (1996) Ectotherm life-history responses to developmental temperature. In: Johnston IA & AF. Bennet (Eds.), Animals and temperature phenotypic and evolutionary adaptation. Cambridge University Press, Cambridge, United Kingdom, pp. 183–204.
Bandelt, H.J., Forster, P. & Rohl, A. (1999) Median-joining networks for inferring intraspecific phylogenies. Molecular Biology and Evolution, 16, 37–48.
Basso, N.G. (1998) A new telmatobiine leptodactylid frog of the genus Atelognathus from Patagonia. Herpetologica, 54, 44–52.
Brieva, L.M. & Formas, J.R. (2001) Allozyme variation and geographic differentiation in the Chilean leptodactylid frog Batrachyla taeniata (Girard, 1854). Amphibia-Reptilia, 22, 413–420.
Cei, J.M. (1962) Batracios de Chile. Ediciones Universidad de Chile, Santiago. cviii + 128 pp.
Cei, J.M. (1980) Amphibians of Argentina. Monitore zoologico italiano N.S. Monogr. 2, xii + 609 pp.
Cei, J.M. (1984) A new leptodactylid frog, genus Atelognathus, from southern Patagonia, Argentina. Herpetologica, 40, 47–51.
Cei, J.M. & Roig, V.G. (1968) Telmatobiinos de las lagunas basálticas de Neuquén (Anura, Leptodactylidae). Physis (Buenos Aires), 27, 265–284.
Crawford, A.J. (2003) Relative rates of nucleotide substitution in frogs. Journal of Molecular Evolution, 57, 636–641.
Crump, M.L. (1989) Effect of habitat drying on development time and size at metamorphosis in Hyla pseudopuma. Copeia, 1989, 794–797.
Díaz-Páez, H., Núñez, J., Núñez, H. & Ortiz, J.C. (2008) Estado de conservación de los herpetozoos en Chile. In: Vidal, M. & Labra, M.A. (Eds), Herpetología de Chile. Science Verlag Ediciones, Santiago, Chile, pp 233–268.
Denton, G.H., Heusser, C.J., Lowell, T.V., Moreno, P.I., Andersen, B.G., Heusser, L.E., Schuchter, C. & Marchant, D.R. (1999) Interhemispheric linkage of paleoclimate during the last glaciation. Geografiska Annaler, 81A, 107–153.
Donoso-Barros, R. (1966) Reptiles de Chile. Ediciones Universidad de Chile, Santiago de Chile. cxxxvi + 458 pp.
Dupanloup, I., Schneider, S. & Excoffier, L. (2002) A simulated annealing approach to define the genetic structure of populations. Molecular Ecology, 11, 2571–2581.
Excoffier, L., Smouse, P. & Quattro, J. (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics, 131, 479–491.
Filatov, D.A. (2002) ProSeq: A software for preparation and evolutionary analysis of DNA sequence data sets. Molecular Ecology Notes, 2, 621–624.
Fu, Y.X. (1997) Statistical neutrality of mutations against population growth, hitchhiking and background selection. Genetics, 147, 915–925.
Goebel, A.M., Donnelly, J.M. & Atz, M.E. (1999) PCR primers and amplification methods for 12S ribosomal DNA, the Control Region, Cytochrome Oxidase I, and Cytochrome b in Bufonids and others frogs, and a overview of PCR primers which have amplified DNA in Amphibians successfully. Molecular Phylogenetics and Evolution, 11, 163–199.
Heusser, C.J., Heusser, L.E. & Lowell, T.V. (1999) Paleoecology of the southern Chilean Lake District-Isla Grande de Chiloé during middle-Late Llanquihue glaciation and deglaciation. Geografiska Annaler, 81A, 231–284.
Hudson, R.R., Slatkin, M. & Maddison, W.P. (1992) Estimation of levels of gene flow from DNA sequence data. Genetics, 132, 583–589.
Lamy, F., Kaiser, J., Arz, H.W., Hebbeln, D., Ninnemann, U., Timm, O., Timmermann, A. & Toggweiler, J.R. (2007) Modulation of the bipolar seesaw in the Southeast Pacific during Termination 1. Earth and Planetary Science Letters, 259, 400–413.
Liebers, D. & Helbig, J. (2002) Phylogeography and colonization history of Lesser Black-backed gulls (Larus fuscus) as revealed by mtDNA sequences. Journal of Evolutionary Biology, 15, 1021–1033.
Lynch, J.D. (1975) A new chilean frog of the extra-Andean assemblage of Telmatobius (Amphibia: Leptodactylidae). Southern California Academy of Sciences Bulletin, 74, 160–161.
Lynch, J.D. (1978) A re-assessment of the Telmatobiine leptodactylid frogs of Patagonia. Occasional Papers of the Museum of Natural History. The University of Kansas. Lawrence, Kansas, 72, 1–57.
Lumley, S.H. & Switsur, R. (1993) Late Quaternary chronology of the Taitao Peninsula, southern Chile. Journal of Quaternary Science, 8, 161–165.
Markgraf, V., Romero, E. & Villagrán, C. (1996) History and paleoecology of South American Nothofagus Forests. In: Veblen, T.T., Hill, R.S. & Read, J. (Eds), The ecology and biogeography of Nothofagus forests. Yale University Press, New Haven., pp 354–386.
Mc Culloch, R.D., Bentley, M.J., Purves, R.S., Hulton, N.R.J., Sugden, D.E. & Clapperton, C.M. (2000) Climatic inferences from glacial and palaeoecological evidence at the last glacial termination, southern South America. Journal of quaternary Science, 15, 409–417.
Menéndez, C.A. (1969) Die fossilen floren Sudamerikas. In: Fittkau, E.J., Illies, H., Klinge, H., Schwabe, G.H. & Sioli, H. (Eds). Biogeography and Ecology in South America, 2. Dr. W. Junk, The Hague, The Netherlands, pp 519–561.
Meriggio, V., Veloso, A., Young, S. & Nuñez, H. (2004) Atelognathus jeinimenensis n.sp. de Leptodactylidae para el Sur de Chile. Boletín del Museo Nacional de Historia Natural (Chile), 53, 99–123.
Moreno, P.I. & Leon, A.L. (2003) Abrupt vegetation changes during the last glacial to Holocene transition in mid-latitude South America. Journal of Quaternary Science, 18, 787–800.
Moreno, P.I., Jacobson, G.L., Andersen, B., Lowell, T.V. & Denton, G.H. (1999) Abrupt vegetation and climate changes during the last glacial maximum and the last Termination in the Chilean Lake District: A case study from Canal de la Puntilla (41ºS). Geografiska Annaler, 81 A, 285–311.
Newman, R.A. (1989) development plasticity of Scaphiopus couchii tadpoles in an unpredictable environment. Ecology, 70, 1775–1787.
Premoli, A.C., Kitzberger, T. & Veblen, T.T. (2000) Isozyme variation and recent biogeographical history of the long-lived conifer Fitzroya cupressoides. Journal of Biogeography, 27, 251–260.
Ramos-Onsins, S.E. & Rozas, J. (2002) Statistical properties of new neutrality tests against population growth. Molecular Biology and Evolution, 19, 2092–2100.
Rivas, A. (2009) Taller estadística descriptiva multivariada. Análisis de componentes principales. Dirección de Docencia. Universidad de Concepción, 33pp.
Rozas, J., Sanchez-DelBarrio, J.C., Messeguer, X. & Rozas, R. (2003) DnaSP, DNA polymorphism analyses by the coalescent and other methods. Bioinformatics, 19, 2496–2497.
Schneider, S., Roessli, D. & Excoffier, L. (2000) Arlequin: A software for population genetic data. Genetics and Biometry Laboratory, University of Geneva, Switzerland.
Tajima, F. (1989) Statistical method for testing the neutral mutation hypothesis by DNA polymorphism. Genetics, 123, 585–595.
Veloso, A. & Navarro, J. (1988) Lista sistemática y distribución geográfica de anfibios y reptiles de Chile. Bollettino del Museo Regionale di Scienze Naturali, Torino, 6, 481–539.
Victoriano, P., Ortiz, J.C., Benavides, E., Adams, B.J. & Sites, J.W. (2008) Comparative phylogeography of codistributed species of Chilean Liolaemus (Squamata: Tropiduridae) from the central-southern Andean range. Molecular Ecology, 17, 2397–2416.
Vidal, M.A. (2008) Biogeografía de Anfibios y Reptiles. In Vidal, M.A. & Labra, M.A. (Eds), Herpetología de Chile. Science Verlag Ediciones, pp 195–231.
Villagrán, C., Armesto, J.J. & Leiva, R. (1986) Recolonización postglacial de Chiloé insular: evidencias basadas en la distribución geográfica y los modos de dispersión de la flora. Revista Chilena de Historia Natural, 59, 19–39.