Skip to main content Skip to main navigation menu Skip to site footer
Type: Article
Published: 2022-11-29
Page range: 149-157
Abstract views: 35
PDF downloaded: 2

A new species of Neoconidiobolus (Entomophthorales, Ancylistaceae) from China based on morphological, molecular and physiological evidences

Anhui Provincial Key Laboratory for Microbial Pest Control, Anhui Agricultural University, Hefei 230036, China; School of Civil Engineering and Architecture, Anhui University of Technology, Ma,anshan 243002, China.
School of Civil Engineering and Architecture, Anhui University of Technology, Ma,anshan 243002, China;
School of Civil Engineering and Architecture, Anhui University of Technology, Ma,anshan 243002, China;
Institute of Microbiology, School of Ecology and Nature Conservation, Beijing Forestry University, Beijing 100083, China;
College of Life Sciences, Shandong Normal University, Jinan 250358, China;
Anhui Provincial Key Laboratory for Microbial Pest Control, Anhui Agricultural University, Hefei 230036, China;
Entomophthoromycota Taxonomy Phylogeny New taxon Fungi

Abstract

Neoconidiobolus pseudothromboides is proposed as a species new on the basis of morphological characteristics, physiological feature and molecular phylogeny. This new species is morphologically allied to N. thromboides. However, the maximum growth temperature of this new species is lower than that of N. thromboides and it grows slower than N. thromboides. The phylogenetic analysis of mitochondrial small subunit (mtSSU), nuclear large subunit (nucLSU) and translation elongation-factor-like (EFL) reveals that Neoconidiobolus is divided into three clades, and N. pseudothromboides sp. nov. is closely related to N. lachnodes rather than N. thromboides. Morphological comparisons between N. pseudothromboides sp. nov. and its affinities are provided herein.

References

<p>Cai, Y., Nie, Y., Zhao, H., Wang, Z.M., Zhou, Z.Y., Liu, X.Y. &amp; Huang, B. (2021) Azygosporus gen. nov., a synapmorphic clade in the family Ancylistaceae. Mycokeys 85 (2): 161–172. https://doi.org/10.3897/mycokeys.85.73405<br>Chen, C., Ye, S.D., Wang, D.Q., Hatting, J.L. &amp; Yu, X.P. (2014) Alginate embedding and subsequent sporulation of in vitro-produced Conidiobolus thromboides hyphae using a pressurised air-extrusion method. Biological Control 69: 52–58. https://doi.org/10.1016/j.biocontrol.2013.10.016<br>Darriba, D., Taboada, G.L., Doallo, R. &amp; Posada, D. (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature Methods 9 (8): 772. https://doi.org/10.1038/nmeth.2109<br>Drechsler, C. (1952) Widespread distribution of Delacroixia coronata and other saprophytic Entomophthoraceae in plant detritus. Science 115: 575–576. https://doi.org/10.1126/science.115.2995.575<br>Drechsler, C. (1953) Three new species of Conidiobolus isolated from leaf mold. Journal of the Washington Academy of Science 43 (2): 29–34.<br>Drechsler, C. (1954) Two species of Conidiobolus with minutely ridged zygospores. American Journal of Botany 41: 567–575. https://doi.org/10.1002/j.1537-2197.1954.tb14380.x<br>Drechsler, C. (1955) Three new species of Conidiobolus isolated from decaying plant detritus. American Journal of Botany 42 (5): 437–443. https://doi.org/10.1002/j.1537-2197.1955.tb11144.x<br>Drechsler, C. (1960) Two new species of Conidiobolus found in plantdetritus. American Journal of Botany 47: 368–377. https://doi.org/10.1002/j.1537-2197.1960.tb07138.x<br>Drechsler, C. (1965) A robust Conidiobolus with zygospores containing granular parietal protoplasm. Mycologia 57 (6): 913–926. https://doi.org/10.2307/3756891<br>Edgar, R.C. (2004) MUSCLE: Multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32: 1792–1797. https://doi.org/10.1093/nar/gkh340<br>Gryganskyi, A.P., Humber, R.A., Smith, M.E., Hodge, K., Huang, B., Voigt, K. &amp; Vilgalys, R. (2013) Phylogenetic lineages in Entomophthoromycota. Persoonia 30: 94–105. https://doi.org/10.3767/003158513X666330<br>Guindon, S. &amp; Gascuel, O. (2003) A simple, fast and accurate method to estimate large phylogenies by maximum-likelihood. Systematic Biology 52: 696–704. https://doi.org/10.1080/10635150390235520<br>Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98<br>Humber, R.A. (2012) Entomophthoromycota: a new phylum and reclassification for entomophthoroid fungi. Mycotaxon 120: 477–492. https://doi.org/10.5248/120.477<br>King, D.S. (1976) Systematics of Conidiobolus (Entomophthorales) usingnumerical taxonomy I. Taxonomic considerations. Canadian Journal of Botany 54: 45–65. https://doi.org/10.1139/b76-008<br>King, D.S. (1977) Systematics of Conidiobolus (Entomophthorales) using numerical taxonomy III. Descriptions of recognized species. Canadian Journal of Botany 55:718–729. https://doi.org/10.1139/b77-086<br>Michal, H., Ryszard, M. &amp; Jacek, P.T. (2014) The effect of Cry1AB insecticidal protein on the incidence of entomopathogenic fungi infecting aphids on Bt maize. Zemdirbyste-agriculture 101 (3): 279–284. https://doi.org/10.13080/z-a.2014.101.036<br>Nie, Y., Yu, C.Z., Liu, X.Y. &amp; Huang, B. (2012) A new species of Conidiobolus (Ancylistaceae) from Anhui, China. Mycotaxon 120: 427–435. https://doi.org/10.5248/120.427<br>Nie, Y., Tang, X.X., Liu, X.Y. &amp; Huang, B. (2016) Conidiobolus stilbeus, a new species with mycelial strand and two types of primary conidiophores. Mycosphere 7 (6): 801–809. https://doi.org/10.5943/mycosphere/7/6/11<br>Nie, Y., Tang, X.X., Liu, X.Y. &amp; Huang, B. (2017) A new species of Conidiobolus with chlamdosporus from Dabie Mountains, eastern China. Mycosphere 8 (7): 809–816. https://doi.org/10.5943/mycosphere/8/7/1<br>Nie, Y., Qin, L., Yu, D.S., Liu, X.Y. &amp; Huang, B. (2018) Two new species of Conidiobolus occurring in Anhui, China. Mycological Progress 17 (10): 1203–1211. https://doi.org/10.1007/s11557-018-1436-z<br>Nie, Y., Yu, D.S., Wang, C.F., Liu, X.Y. &amp; Huang, B. (2020a) A taxonomic revision of the genus Conidiobolus (Ancylistaceae, Entomophthorales): four clades including three new genera. Mycokeys 66: 55–81. https://doi.org/10.3897/mycokeys.66.46575<br>Nie, Y., Cai, Y., Gao, Y., Yu, D.S., Wang, Z.M., Liu, X.Y. &amp; Huang, B. (2020b) Three new species of Conidiobolus sensu stricto from plant debris in eastern China. MycoKeys 73: 133–149. https://doi.org/10.3897/mycokeys.73.56905<br>Nie, Y., Wang, Z.M., Liu, X.Y. &amp; Huang, B. (2021) A morphological and molecular survey of Neoconidiobolus reveals a new species and two new combinations. Mycological Porgress 20 (10): 1233–1241. https://doi.org/10.1007/s11557-021-01720-w <br>Nie, Y., Zhao, H., Wang, Z.M., Zhou, Z.Y., Liu, X.Y. &amp; Huang, B. (2022) Two new species in Capillidium (Ancylistaceae, Entomophthorales) from China, with a proposal for a new combination. Mycokeys 89: 139–153. https://doi.org/10.3897/mycokeys.89.79537<br>Rambaut, A. (2012) FigTree version 1.4.0. Available at: http://tree.bio.ed.ac.uk/software/figtree/ (accessed 28 November 2022)<br>Ronquist, F. &amp; Huelsenbeck, J.P. (2003) MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572–1574. https://doi.org/10.1093/bioinformatics/btg180<br>Srinivasan, M.C. &amp; Thirumalachar, M.J. (1962) Studies on species of Conidiobolus from India-II. Sydowia, Annales Mycologici 16: 60–66<br>Srinivasan, M.C. &amp; Thirumalachar, M.J. (1968) Two new species of Conidiobolus from India. Journal of the Mitchell Society 84: 211–212<br>Stamatakis, A. (2014) RAxML version 8: A tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30 (9): 1312–1313. https://doi.org/10.1093/bioinformatics/btu033<br>Swofford, D.L. (2002) PAUP*: Phylogenetic analysis using parsimony (*and other methods), Version 4.0b10. Sinauer Associates, Sunderland<br>Vaidya, G., Lohman, D.J. &amp; Meier, R. (2011) SequenceMatrix: concatenation software for the fast assembly of multi-gene datasets with character set and codon information. Cladistics 27 (2): 171–180. https://doi.org/10.1111/j.1096-0031.2010.00329.x<br>Vilela, R., Silva, S.M.S., Correa, F.R., Dominguez, E. &amp; Mendoza, L. (2010) Morphologic and phylogenetic characterization of Conidiobolus lamprauges recovered from infected sheep. Journal of Clinical Microbiology 48: 427–432. https://doi.org/10.1128/JCM.01589-09<br>Vilgalys, R. &amp; Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990<br>Waingankar, V.M., Singh, S.K. &amp; Srinivasan, M.C. (2008) A new thermophilic species of Conidiobolus from India. Mycopathologia 165: 173–177. https://doi.org/10.1007/s11046-007-9088-6<br>Watanabe, M., Lee, K., Goto, K., Kumagai, S., Sugita-Konishi, Y. &amp; Hara-Kudo, Y. (2010) Rapid and effective DNA extraction method with bead grinding for a large amount of fungal DNA. Journal of Food Protection 73 (6): 1077–1084. https://doi.org/10.4315/0362-028X-73.6.1077<br>Waters, S.D. &amp; Callaghan, A.A. (1989) Conidiobolus iuxtagenitus, a new species with discharge delongate repetitional conidia and conjugation tubes. Mycological Research 93: 223–226. https://doi.org/10.1016/S0953-7562(89)80121-2<br>Zhao, H., Nie, Y., Zong, T.K., Dai, Y.C. &amp; Liu, X.Y. (2022) Three New Species of Absidia (Mucoromycota) from China Based on Phylogeny, Morphology and Physiology. Diversity 14 (2): 132. https://doi.org/10.3390/d14020132<br>Zheng, R.Y., Chen, G.Q., Huang, H. &amp; Liu, X.Y. (2007) A monograph of Rhizopus. Sydowia 59: 273.<br>Zheng, R.Y. &amp; Liu, X.Y. (2009) Taxa of Pilaira (Mucorales, Zygomycota) from China. Nova Hedwig 88: 255–267. https://doi.org/10.1127/0029-5035/2009/0088-0255<br>Zheng, R.Y., Liu, X.Y. &amp; Li, R.Y. (2009) More Rhizomucor causing human mucormycosis from China: R. chlamydosporus sp. nov. Sydowia 61: 135–147.<br>Zoller, S., Scheideggera, C. &amp; Sperisena, C. (1999) PCR primers for the amplification of mitochondrial small subunit ribosomal DNA of lichen-forming ascomycetes. Lichenologist 31 (5): 511–516. https://doi.org/10.1006/lich.1999.0220</p>