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Published: 2022-08-08

Colletotrichum juglandis sp. nov. (Ascomycota: Glomerellaceae) associated with walnut leaf spot in China

Department of Plant Protection, College of Agriculture, Yangtze University, Jingzhou 434025, China
Department of Plant Protection, College of Agriculture, Yangtze University, Jingzhou 434025, China
Department of Plant Protection, College of Agriculture, Yangtze University, Jingzhou 434025, China
Animal and Plant Quarantine and Testing Center, Jingzhou 434020, China
Department of Plant Protection, College of Agriculture, Yangtze University, Jingzhou 434025, China
Department of Horticulture, College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China
Walnut (Juglans regia) Colletotrichum Phylogenetic analysis New species Taxonomy Fungi


Colletotrichum species are destructive pathogens, saprobes and endophytes on various plant hosts. However, the species occurring on walnut remains largely unresolved. Walnut leaf spot occurred seriously in orchards of Xingshan County, Hubei Province in China. To determine Colletotrichum spp. associated with Juglans regia, 22 isolates were obtained from the diseased leaf samples collected during 2019 and 2020. Seven isolates were further selected as representatives based on morphology and phylogeny using ITS, GAPDH, CHS-1 and ACT gene regions. The results revealed that the isolates belonged to two phylogenetic species complexes, C. gloeosporioides species complex (CGSC) comprising C. gloeosporioides and C. kahawae, and C. acutatum species complex (CASC) including C. nymphaeae, C. godetiae, C. fioriniae and a new species, namely Colletotrichum juglandis sp. nov.. Among them, C. kahawae was firstly reported from walnut.


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