Abstract
Sinocrassula jiaozishanensis, a new species from China, is described and illustrated. Molecular analysis and morphological comparisons were carried out. The new species can be easily distinguished from other Sinocrassula species by its basal leaves opposite, not rosulate, turquoise with apex red, flowering stems terminal, and nectar scales oblong to kidney-shaped.
References
<p>Arriba, D.D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: more models, new heuristics and parallel computing. <em>Nature Methods </em>9: 772. https://doi.org/10.1038/nmeth.2109</p>
<p>Averyanov, L.V., Byalt, V.V., Vinh, N.T., Loc, P.K. & Hieu, N.Q. (2014) Sinocrassula vietnamenis (Crassulaceae), new species and new generic record in the flora of Vietnam. <em>Taiwania </em>59: 348–352. https://doi.org/10.6165/tai.2014.59.4.348</p>
<p>Chen, S.L., Yao, H., Han, J.P, Liu, C., Song, J.Y., Shi, L.C., Zhu, Y.J., Ma, X.Y., Gao, T., Pang, X.H., Luo, K., Li, Y., Li, X.W., Jia, X.C. & Leon, C. (2010) Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species. <em>Plos One</em> 5 (1): e8613. https://doi.org/10.1371/journal.pone.0008613</p>
<p>Group, C. (2009) A DNA barcode for land plants. <em>Proceedings of the National Academy of Sciences of the United States of America </em>106: 12794–12797. https://doi.org/10.1073/pnas.0905845106</p>
<p>Fu, K.J. & Ohba Hideaki. (2013) Crassulaceae. <em>In:</em> Wu, Z.Y. & Raven, P.H. (Eds.) <em>Flora of China</em> Vol. 8. Science Press, Beijing and Missouri Botanical Garden Press, St. Louis, pp. 202–268.</p>
<p>Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/nt. <em>Nuclc Acids Symposium Series</em> 41: 95–98. https://doi.org/10.1021/bk-1999-0734.ch008</p>
<p>IUCN (2017) The IUCN red list of threatened species, version 2017. 1. IUCN Red List Unit. Available from: http://www.iucnredlist.org/ (accessed 10 April 2017)</p>
<p>Katoh, K. & Standley, D.M. (2013) MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability. <em>Molecular Biology and Evolution </em>30: 772–780. https://doi.org/10.1093/molbev/mst010</p>
<p>Kress, W.J. & Erickson, D.L. (2007) A Two-Locus Global DNA Barcode for Land Plants: The Coding <em>rbcL</em> Gene Complements the Non-Coding <em>trnH-psbA</em> Spacer Region. <em>Plos One </em>2. https://doi.org/10.1371/journal.pone.0000508</p>
<p>Mayuzumi, S. & Ohba, H. (2004) The phylogenetic position of eastern Asian Sedoideae (Crassulaceae) inferred from chloroplast and nuclear DNA sequences. <em>Systematic Botany </em>29: 587–598. https://doi.org/10.1600/0363644041744329</p>
<p>Messerschmid, T.F.E., Klein, J.T., Kadereit, G. & Kadereit, J.W. (2020) Linnaeus’s folly phylogeny, evolution and classification ofSedum(Crassulaceae) and Crassulaceae subfamily Sempervivoideae. <em>Taxon </em>69: 892–926. https://doi.org/10.1002/tax.12316</p>
<p>Miller, M.A., Pfeiffer, W.T. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for Inference of Large Phylogenetic Trees. <em>In: Proceedings of the Gateway Computing Environments Workshop (GCE)</em>. IEEE Computer Society. https://doi.org/10.1109/GCE.2010.5676129</p>
<p>Rambaut A, Drummond AJ (2007) Tracer v1.4. Tracer Release website. Available: [http://beast.bio.ed.ac.uk/Tracer]</p>
<p>Ronquist, F. & Huelsenbeck, J.P. (2003) MRBAYES3: Bayesian phylogenetic inference under mixed models. <em>Bioinformatics</em> 19: 1572–1574. https://doi.org/10.1371/journal.pone.0049090</p>
<p>Sang, T., Crawford, D.J. & Stuessy, T.F. (1997) Chloroplast DNA phylogeny, reticulate evolution, and biogeography of Paeonia (Paeoniaceae). <em>American Journal of Botany </em>84: 1120–1136. https://doi.org/10.2307/2446155</p>
<p>Stamatakis, A., Hoover, P. & Rougemont, J. (2008) A Rapid Bootstrap Algorithm for the RAxML Web Servers. <em>Systematic Biology </em>57: 758–771. https://doi.org/10.1080/10635150802429642</p>
<p>Taberlet, P., Gielly, L., Pautou, G. & Bouvet, J. (1991) Universal primers for amplification of 3 noncoding regions of chloroplast DNA. <em>Plant Mol Biol</em> 17: 1105–1109. https://doi.org/10.1007/BF00037152</p>
<p>Thiers, B. (2018) Index Herbariorum: A global directory of public herbaria and associated staff. New York Botanical Garden’s Virtual. Herbarium. Available from: http://sweetgum.nybg.org/science/docs/The_Worlds_Herbaria_2018.pdf (accessed 21 March 2022)</p>
<p>Wang, J., Du, F. & Liu, Y. (2012) Sinocrassula stenosquamata (Crassulaceae), A New Species from Yunnan, China. <em>Acta Botanica Boreali-Occidentalia Sinica </em>32: 2563–2565. https://doi.org/10.3969/j.issn.1000-4025.2012.12.030</p>
<p>Zhang, J.-Q., Meng, S.-Y., Wen, J. & Rao, G.-Y. (2015) DNA Barcoding of Rhodiola (Crassulaceae): A Case Study on a Group of Recently Diversified Medicinal Plants from the Qinghai-Tibetan Plateau. <em>PLoS ONE</em> 10 (3): e0119921. https://doi.org/10.1371/journal.pone.0119921</p>
<p>Averyanov, L.V., Byalt, V.V., Vinh, N.T., Loc, P.K. & Hieu, N.Q. (2014) Sinocrassula vietnamenis (Crassulaceae), new species and new generic record in the flora of Vietnam. <em>Taiwania </em>59: 348–352. https://doi.org/10.6165/tai.2014.59.4.348</p>
<p>Chen, S.L., Yao, H., Han, J.P, Liu, C., Song, J.Y., Shi, L.C., Zhu, Y.J., Ma, X.Y., Gao, T., Pang, X.H., Luo, K., Li, Y., Li, X.W., Jia, X.C. & Leon, C. (2010) Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species. <em>Plos One</em> 5 (1): e8613. https://doi.org/10.1371/journal.pone.0008613</p>
<p>Group, C. (2009) A DNA barcode for land plants. <em>Proceedings of the National Academy of Sciences of the United States of America </em>106: 12794–12797. https://doi.org/10.1073/pnas.0905845106</p>
<p>Fu, K.J. & Ohba Hideaki. (2013) Crassulaceae. <em>In:</em> Wu, Z.Y. & Raven, P.H. (Eds.) <em>Flora of China</em> Vol. 8. Science Press, Beijing and Missouri Botanical Garden Press, St. Louis, pp. 202–268.</p>
<p>Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/nt. <em>Nuclc Acids Symposium Series</em> 41: 95–98. https://doi.org/10.1021/bk-1999-0734.ch008</p>
<p>IUCN (2017) The IUCN red list of threatened species, version 2017. 1. IUCN Red List Unit. Available from: http://www.iucnredlist.org/ (accessed 10 April 2017)</p>
<p>Katoh, K. & Standley, D.M. (2013) MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability. <em>Molecular Biology and Evolution </em>30: 772–780. https://doi.org/10.1093/molbev/mst010</p>
<p>Kress, W.J. & Erickson, D.L. (2007) A Two-Locus Global DNA Barcode for Land Plants: The Coding <em>rbcL</em> Gene Complements the Non-Coding <em>trnH-psbA</em> Spacer Region. <em>Plos One </em>2. https://doi.org/10.1371/journal.pone.0000508</p>
<p>Mayuzumi, S. & Ohba, H. (2004) The phylogenetic position of eastern Asian Sedoideae (Crassulaceae) inferred from chloroplast and nuclear DNA sequences. <em>Systematic Botany </em>29: 587–598. https://doi.org/10.1600/0363644041744329</p>
<p>Messerschmid, T.F.E., Klein, J.T., Kadereit, G. & Kadereit, J.W. (2020) Linnaeus’s folly phylogeny, evolution and classification ofSedum(Crassulaceae) and Crassulaceae subfamily Sempervivoideae. <em>Taxon </em>69: 892–926. https://doi.org/10.1002/tax.12316</p>
<p>Miller, M.A., Pfeiffer, W.T. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for Inference of Large Phylogenetic Trees. <em>In: Proceedings of the Gateway Computing Environments Workshop (GCE)</em>. IEEE Computer Society. https://doi.org/10.1109/GCE.2010.5676129</p>
<p>Rambaut A, Drummond AJ (2007) Tracer v1.4. Tracer Release website. Available: [http://beast.bio.ed.ac.uk/Tracer]</p>
<p>Ronquist, F. & Huelsenbeck, J.P. (2003) MRBAYES3: Bayesian phylogenetic inference under mixed models. <em>Bioinformatics</em> 19: 1572–1574. https://doi.org/10.1371/journal.pone.0049090</p>
<p>Sang, T., Crawford, D.J. & Stuessy, T.F. (1997) Chloroplast DNA phylogeny, reticulate evolution, and biogeography of Paeonia (Paeoniaceae). <em>American Journal of Botany </em>84: 1120–1136. https://doi.org/10.2307/2446155</p>
<p>Stamatakis, A., Hoover, P. & Rougemont, J. (2008) A Rapid Bootstrap Algorithm for the RAxML Web Servers. <em>Systematic Biology </em>57: 758–771. https://doi.org/10.1080/10635150802429642</p>
<p>Taberlet, P., Gielly, L., Pautou, G. & Bouvet, J. (1991) Universal primers for amplification of 3 noncoding regions of chloroplast DNA. <em>Plant Mol Biol</em> 17: 1105–1109. https://doi.org/10.1007/BF00037152</p>
<p>Thiers, B. (2018) Index Herbariorum: A global directory of public herbaria and associated staff. New York Botanical Garden’s Virtual. Herbarium. Available from: http://sweetgum.nybg.org/science/docs/The_Worlds_Herbaria_2018.pdf (accessed 21 March 2022)</p>
<p>Wang, J., Du, F. & Liu, Y. (2012) Sinocrassula stenosquamata (Crassulaceae), A New Species from Yunnan, China. <em>Acta Botanica Boreali-Occidentalia Sinica </em>32: 2563–2565. https://doi.org/10.3969/j.issn.1000-4025.2012.12.030</p>
<p>Zhang, J.-Q., Meng, S.-Y., Wen, J. & Rao, G.-Y. (2015) DNA Barcoding of Rhodiola (Crassulaceae): A Case Study on a Group of Recently Diversified Medicinal Plants from the Qinghai-Tibetan Plateau. <em>PLoS ONE</em> 10 (3): e0119921. https://doi.org/10.1371/journal.pone.0119921</p>