Abstract
Euphorbia motuogensis M. T. Li, X. Z. Lan, H. P. Deng & W. L. Zheng, sp. nov., a new species from Motuo, Tibet, China, is described and illustrated here. It is closely similar to Euphorbia sikkimensis in having terete root, alternate leaves, well-developed pseudoumbellate inflorescence, cyathium, smooth and glaborus capsule, but Euphorbia motuogensis is clealy distinguishable by its pilose stems, involucral leaves color, secondary involucral leaves absent, cyathophylls number and color, and five similar glands. Furthermore, molecular phylogenetic analyses of sequences from both nuclear ribosomal ITS confirm that this species is distinct from morphologically similar species in this subgenus.
References
<p>Doyle, J.J. & Doyle, J.L. (1987) A rapid DNA isolation procedure for small quantities of fresh leaf material. <em>Phytochem Bull</em> 19: 11–15.</p>
<p>Horn, J.W., Ee, B.W.V., Morawetz, J.J., Riina, R., Steinmann, V.W. & Berry, P.E. (2012) Phylogenetics and the evolution of major structural characters in the giant genus <em>Euphorbia</em> L. (Euphorbiaceae). <em>Molecular Phylogenetics & Evolution</em> 63 (2): 305–326. https://doi.org/10.1016/j.ympev.2011.12.022</p>
<p>Higgins, D.G., Thompson, J.D., Gibson, T.J. (1994) The Clustal W: inproving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. <em>Nucleic Acids Research</em> 22: 4673–4680. https://doi.org/10.1093/nar/22.22.4673</p>
<p>Ma, J.-S. & Gilbert, G.M. (2008) <em>Euphorbia</em> <em>In</em>: Wu, Z.Y., Raven, P.R. & Hong, D.Y. (eds.) <em>Flora of China</em> 11. Science Press, Beijing & Missouri Botanical Garden, St. Louis, pp. 288–313.</p>
<p>Ma, J.-S., Liu, Y & Wei, F.-N. (2013) A new species of <em>Euphorbia</em> (Euphorbiaceae) from Guangxi, China. <em>Phytotaxa</em> 87 (3): 45–49. https://doi.org/10.11646/phytotaxa.87.3.2</p>
<p>Posada, D. & Crandall, K.A. (1998) Modeltest: testing the model of DNA substitution. <em>Bioinformatics</em> 14: 817–818. https://doi.org/10.1093/bioinformatics/14.9.817</p>
<p>Peirson, J.A., Bruyns, P.V., Riina, R., Morawetz, J.J. & Berry, P.E. (2013) A molecular phylogeny and classification of the largely succulent and mainly African <em>Euphorbia</em> subg. <em>Athymalus</em>(Euphorbiaceae). <em>Taxon</em> 62 (6): 1178–1199. https://doi.org/10.12705/626.12</p>
<p>Riina, R., Peirson, J.A., Geltman, D.V., Molero, J., Frajman, B., Pahlevani, A., Barres, L., Morawetz, J., Salmaki, Y., Zarre, S., Kryukov, A., Bruyns, P. & Berry, P.E. (2013) A worldwide molecular phylogeny and classification of the leafy spurges, <em>Euphorbia</em> subgenus <em>Esula</em> (Euphorbiaceae). <em>Taxon</em> 62 (2): 316–342. https://doi.org/10.12705/622.3</p>
<p>Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. <em>Bioinformatics</em> 19: 1572–1574. https://doi.org/10.1093/bioinformatics/btg180</p>
<p>Steinmann, V.W. & Porter, J.M. (2002) Phylogenetic relationships in Euphorbieae (Euphorbiaceae) based on its and ndhf sequence data. <em>Annals of the Missouri Botanical Garden</em> 89 (4): 453–490. https://doi.org/10.2307/3298591</p>
<p>Tian, X.M., Wang, Q.Y. & Zhou, Y.F. (2018) <em>Euphorbia</em> section <em>hainanensis</em> (Euphorbiaceae), a new section endemic to the hainan island of china from biogeographical, karyological, and phenotypical evidence. <em>Frontiers Plant Science</em> 9 (2). https://doi.org/10.3389/fpls.2018.00660</p>
<p>White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics.<em> In: </em>Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) <em>PCR Protocols: A Guide to Methods and Applications. </em>Academic Press, New York, pp. 315–322.</p>
<p> ttps://doi.org/10.1016/B978-0-12-372180-8.50042-1</p>
<p>Yang, Y., Riina, R., Morawetz, J.J., Haevermans, T., Aubriot, X. & Berry, P.E. (2012) Molecular phylogenetics and classification of <em>Euphorbia</em> subgenus <em>Chamaesyce</em> (Euphorbiaceae). <em>Taxon</em> 61 (4): 764–789. https://doi.org/10.3732/ajb.1000496</p>
<p>Horn, J.W., Ee, B.W.V., Morawetz, J.J., Riina, R., Steinmann, V.W. & Berry, P.E. (2012) Phylogenetics and the evolution of major structural characters in the giant genus <em>Euphorbia</em> L. (Euphorbiaceae). <em>Molecular Phylogenetics & Evolution</em> 63 (2): 305–326. https://doi.org/10.1016/j.ympev.2011.12.022</p>
<p>Higgins, D.G., Thompson, J.D., Gibson, T.J. (1994) The Clustal W: inproving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. <em>Nucleic Acids Research</em> 22: 4673–4680. https://doi.org/10.1093/nar/22.22.4673</p>
<p>Ma, J.-S. & Gilbert, G.M. (2008) <em>Euphorbia</em> <em>In</em>: Wu, Z.Y., Raven, P.R. & Hong, D.Y. (eds.) <em>Flora of China</em> 11. Science Press, Beijing & Missouri Botanical Garden, St. Louis, pp. 288–313.</p>
<p>Ma, J.-S., Liu, Y & Wei, F.-N. (2013) A new species of <em>Euphorbia</em> (Euphorbiaceae) from Guangxi, China. <em>Phytotaxa</em> 87 (3): 45–49. https://doi.org/10.11646/phytotaxa.87.3.2</p>
<p>Posada, D. & Crandall, K.A. (1998) Modeltest: testing the model of DNA substitution. <em>Bioinformatics</em> 14: 817–818. https://doi.org/10.1093/bioinformatics/14.9.817</p>
<p>Peirson, J.A., Bruyns, P.V., Riina, R., Morawetz, J.J. & Berry, P.E. (2013) A molecular phylogeny and classification of the largely succulent and mainly African <em>Euphorbia</em> subg. <em>Athymalus</em>(Euphorbiaceae). <em>Taxon</em> 62 (6): 1178–1199. https://doi.org/10.12705/626.12</p>
<p>Riina, R., Peirson, J.A., Geltman, D.V., Molero, J., Frajman, B., Pahlevani, A., Barres, L., Morawetz, J., Salmaki, Y., Zarre, S., Kryukov, A., Bruyns, P. & Berry, P.E. (2013) A worldwide molecular phylogeny and classification of the leafy spurges, <em>Euphorbia</em> subgenus <em>Esula</em> (Euphorbiaceae). <em>Taxon</em> 62 (2): 316–342. https://doi.org/10.12705/622.3</p>
<p>Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. <em>Bioinformatics</em> 19: 1572–1574. https://doi.org/10.1093/bioinformatics/btg180</p>
<p>Steinmann, V.W. & Porter, J.M. (2002) Phylogenetic relationships in Euphorbieae (Euphorbiaceae) based on its and ndhf sequence data. <em>Annals of the Missouri Botanical Garden</em> 89 (4): 453–490. https://doi.org/10.2307/3298591</p>
<p>Tian, X.M., Wang, Q.Y. & Zhou, Y.F. (2018) <em>Euphorbia</em> section <em>hainanensis</em> (Euphorbiaceae), a new section endemic to the hainan island of china from biogeographical, karyological, and phenotypical evidence. <em>Frontiers Plant Science</em> 9 (2). https://doi.org/10.3389/fpls.2018.00660</p>
<p>White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics.<em> In: </em>Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) <em>PCR Protocols: A Guide to Methods and Applications. </em>Academic Press, New York, pp. 315–322.</p>
<p> ttps://doi.org/10.1016/B978-0-12-372180-8.50042-1</p>
<p>Yang, Y., Riina, R., Morawetz, J.J., Haevermans, T., Aubriot, X. & Berry, P.E. (2012) Molecular phylogenetics and classification of <em>Euphorbia</em> subgenus <em>Chamaesyce</em> (Euphorbiaceae). <em>Taxon</em> 61 (4): 764–789. https://doi.org/10.3732/ajb.1000496</p>