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Article
Published: 2021-11-11

A revised infrageneric classification of Bellevalia Lapeyr. (Asparagaceae: Scilloideae) based on molecular analysis

Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
Department of Biology, Faculty of Sciences, Damghan Branch, Islamic Azad University, Damghan, Iran.
Jordell Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey, TW10 7NQ, UK.
Compton Herbarium, South African National Biodiversity Institute, Private Bag X7, Claremont 7735, South Africa; Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal, Pietermaritzburg, Private Bag X01, Scottsville 3209, South Africa.
Asparagaceae Bellevalia Hyacintheae Iran molecular phylogenetics systematics Monocots

Abstract

Within the genus Bellevalia (Asparagaceae: Scilloideae: Hyacintheae), flower shape and colour, the ratio of leaf to scape length, and the orientation of the pedicels were traditionally used to delimit sections, subsections and species. We conducted a phylogenetic analysis of representative species from sections Bellevalia, Conicae, Nutantes and Oxydontae, representing four out of the six sections that are currently recognised, using the four chloroplast regions rbcL, matK, trnL intron, and trnL-F spacer. Our results indicate that the sections are not monophyletic as currently circumscribed. Our analyses retrieve two major, well-supported clades. The first clade (sect. Conicae) includes only species with ciliate leaves and green-veined perianth lobes, and the second clade (sect. Bellevalia) includes the species lacking these characteristics. Within the second clade, the species with yellow anthers are separated from those with violet anthers. Our molecular analysis does not support the traditional subdivisions of the genus but establishes the value of cilia on the leaf margin, green veins in the perianths lobes, and the colour of the anthers as indicators of relationships among the species. We recommend that the current four sections and six subsections of Bellevalia be reduced to two sections without subsections and we provide an updated and corrected nomenclature for these sections and subsections, designating lectotypes where necessary.

References

  1. Akaika, H. (1974) A new look at the statistical model identification. IEEE Trans. Automat. Controi 19: 716–723. https://doi.org/10.1109/TAC.1974.1100705

  2. Altschul, S.F., Miller, G.W., Myers, E.W. & Lipman, D.J. (1990) Basic local alignment search tool. Journal of Molecular Biology 215 (3): 403–410. https://doi.org/10.1016/S0022-2836(05)80360-2

  3. Angiosperm phylogeny Group [APGIV] (2016) An update of the Angiosperm phylogeny Group classification for the orders and families of flowering plants: APG IV. Botanical Journal of the Linnean society 181: 1–20.  https://doi.org/10.1111/boj.12385

  4. Austuti, G., Brullo, S., Domina, G., El Mokni, R., Giordani, T. & Peruzzi, L. (2017) Phylogenetic relationships among tetraploid species of Bellevalia (Asparagaceae) endemic to south-central Mediterranean. Plant Biosystems 151 (6): 1120–1128.  https://doi.org/10.1080/11263504.2017.1320308

  5. Bareka, P., Turland, N.J. & Kamari, G. (2015) Bellevalia juliana (Asparagaceae), a new hexaploid species from E Kriti (Greece). Plant Biosystems 149 (4): 703–709. https://doi.org/10.1080/11263504.2015.1057258

  6. Boissier, E. (1854) Diagnoses Plantarum Orientalium novarum. B. Hermann, Lipsiae, 607 pp.

  7. Boissier, E. (1884) Diagnosis Plantarum Orientales novarum. Bulletin de l‘Herbier Boissier.ser. 1, 5: 300–308. [Geneva]

  8. Bornmüller, J.F.N. (1908) Beihefte zum Botanischen Centralblatt. Abt. 2, Systematik, Pflanzengeographie, angewandte Botanik 24 (2): 108. Kassel. Dresden.

  9. Borzatti von Loewenstern, A., Giordani, T., Astuti, G., Andreucci, A. & Peruzzi, L. (2013) Phylogenetic relationships of Italian Bellevalia species (Asparagaceae), inferred from morphology, karyology and molecular systematics. Plant Biosystems 147: 776–787. https://doi.org/10.1080/11263504.2013.829884

  10.  Cowley, J., Özhatay, N. & Mathew, B. (1994) New species of Alliaceae & Hyacinthaceae from Turkey. Kew Bulletin 49: 481–489.  https://doi.org/10.2307/4114472

  11. Czernik (1923) Hyacinthus litwinowii., Botanicheskie Materialy Gerbariya Glavnogo Botanicheskogo Sada R.S.F.S.R. Petrograd 4: 148.

  12. Doyle, J.J. & Doyle, J.L. (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11–15.

  13. Dunning, L.T. & Savolainen, V. (2010) Broad-scale amplification of matK for DNA barcoding plants, a technical note. Botanical Journal of the Linnean Society 164: 1–9. https://doi.org/10.1111/j.1095-8339.2010.01071.x

  14. Engler, A. (1887) Liliaceae. In: Die NaturlichenPflanzenfamilien, vol. 2 (5). Leipzig, pp. 10–91.

  15. Fay, M.F., Bayer, C., Alverson, W.S., de Bruijn, A.Y. & Chase, M.W. (1998) Plastid rbcL sequence data indicate a close affinity between Diegodendron and Bixa. Taxon 47: 43–50. https://doi.org/10.2307/1224017

  16. Feinbrun, N. (1940) A monographic study of Bellevalia Lapeyr. Palestine journal of botany Jerusalem, ser 1: 336–340

  17. Feinbrun, N. (1986) Flora of Palestine. The Israel academy of science & Humanity. Jerusalem 4: 84–104.

  18. Freitag, H. & Wendelbo, P. (1970) The genus Bellevalia in Afghanistan. Studies in the Flora of Afghanistan. Israel Journal of Botany 19: 220–224.

  19. Govaerts, R. (2018) World checklist of Asparagaceae. Available from: http://wcsp.science.kew.org (accessed 11 November 2021)

  20. Groossheim, A.A. (1928) Flora Kavkaza, vol. 1. Izdatel’stvo Akademii Nauk SSSR, Moskva & Leningrad, 234 pp.

  21. Gürdal, B., Özhatay, N., Koçyiğit, M. & Kaya, E. (2014) Two new species for Turkish geophyte flora in the genus Bellevalia. In: Kaya, E. (Ed.) Türkiye Geofitleri 3, Atatürk Bahçe Kültürleri Merkez Araştırma Enstitüsü, Yalova, pp. 525–531.

  22. Gussone, G. (1841) Muscari neglectum. Annali Civili del Regno delle due Sicilie. Naples 26: 48–49.

  23. Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analyses program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.

  24. Huelsenbeck, J.P. & Ronquist, F. (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17 (8): 754–755. https://doi.org/10.1093/bioinformatics/17.8.754

  25. Jafari, A. & Maassoumi, A.A. (2008) A new species of Bellevalia (Liliaceae/ Hyacinthaceae) from Iran. Edinburgh journal of Botany 65 (3): 469–473. https://doi.org/10.1017/S0960428608005027

  26. Karabacak, O., Yıldırım, H. & Martin, E. (2014) Bellevalia pseudolongipes sp. nov. (Asparagaceae): a new species from southeastern Anatolia, Turkey. Phytotaxa 188 (4): 209–217.  https://doi.org/10.11646/phytotaxa.188.4.3

  27. Karabacak, O., Yıldırım, H. & Martin, E. (2015) Bellevalia koyuncui sp. nova (Asparagaceae): a new species from South Eastern Anatolia, Turkey. Phytotaxa 203 (1): 81–84. https://doi.org/10.11646/phytotaxa.203.1.9

  28. Kunth, C.S. (1843) Bellevalia glauca. Enumeratio Plantarum 4: 309.

  29. Lapeyrouse, P.P. (1808) Bellevalia Lapeyr. Nouveau genre de plante de la famille des Liliacées. Journal de Physique, de Chimie, d’Historie Naturelle et des Arts 67 (12): 425–427.

  30. Linneì, C. (1771) Mantissa plantarum altera. Holmiae : Impensis Direct. Laurentii Salvii, 224 pp.

  31. Losinskaja, A.S.L. (1935) Bellevalia Lapeyr. In: Komarov, V.L. (Ed.) Flora of USSR 4. Akademiya Nauk SSSR., Leningrad, pp. 303–311.

  32. Manning, C., Forest, F., Devey, D.S., Fay, M. & Goldblatt, P. (2009) A molecular phylogeny and a revised classification of Ornithogaloideae (Hyacinthaceae) based on an analysis of four plastid DNA regions. Taxon 58 (1): 1–107.  https://doi.org/10.1002/tax.581011

  33. Müller, K. (2005) SeqState - primer design and sequence statistics for phylogenetic DNA data sets. Applied Bioinformatics 4: 65–69.  https://doi.org/10.2165/00822942-200504010-00008

  34. Nylander, J.A.A. (2004) MrModeltest, version 2. Program distributed by the author. Evolutionary Biology Centre, Uppsala University.

  35. Pallas, P.S. (1927) Hyacinthus sarmaticus Pall. ex Miscz. Trudy Kubanskii Sel’skokhoz. Inst. 5: 155.

  36. Parlatore, F. (1854) Bellevalia webbiana. Nouv. Gen. Sp. Monocot, 19 pp.

  37. Persson, K. & Wendelbo, P. (1979) The taxonomic position of Bellevalia tabriziana (Liliaceae). Botaniska Notiser 132: 197–200.

  38. Pfosser, M. & Speta, F. (1999) Phylogenetic of Hyacinthaceae based on plastid DNA sequences. Annals of the Missouri Botanical Garden 86 (4): 852–875. https://doi.org/10.2307/2666172

  39. Pinar, S.M. & Eroğlu, H. & Fidan, M. (2016) Bellevalia behcetii sp. nov. (Asparagaceae): a new species from South Eastern Anatolia, Turkey. Phytotaxa 270 (2): 127–136.  https://doi.org/10.11646/phytotaxa.270.2.5

  40. Rambaut, A. & Drummond, A. (2009) Tracer. version 1.5. [http://tree.bio.ed.ac.uk/software/ tracer]

  41. Reeves, G., Chase, M.W. Goldblatt, P., Rudall, P., Fay, M.F., Cox, A.V., Lejeune, B. & Souza-Chies, T. (2001) Molecular systematic of Iridaceae: Evidence from four plastid DNA regions. American Journal of Botany 88 (11): 2074–2087.  https://doi.org/10.2307/3558433

  42. Roemer, J.J., Schultes, J.A. & Schultes, J.H. (1830) Systema Vegetabilium ed. 15 bis 7. pp. 586.

  43. Simmons, M.P. & Ochoterena, H. (2000) Gaps as characters in sequence-based phylogenetic analyses. Systematic Biology 49: 369–381. https://doi.org/10.1093/sysbio/49.2.369

  44. Speta, F. (1998) Hyacinthaceae. In: Kubitzki, K. (Ed.) The families and genera of vascular plants. III. Flowering plants. Monocotyledons. (except Orchidaceae). Springer. Berlin, 261–285.  https://doi.org/10.1007/978-3-662-03533-7_35

  45. Taberlet, P., Gielly, L., Pautou, G. & Bouvet, J. (1991) Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Molecular Biology 17: 1105–1109. https://doi.org/10.1007/BF00037152

  46. Thiers, B. (2016 [continuously updated]) Index Herbariorum: a global directory of public herbaria and associated staff. New York Botanical Garden’s Virtual Herbarium. Available from: http://sweetgum.nybg.org/ih/ (accessed 7 May 2016)

  47. Thompson, P.G., Hong, L.L., Ukoskit, K. & Zhu, S. (1994) Genetic linkage of randomly amplified polymorphic DNA (RAPD) markers in sweet potato. Journal of the American Society for Horticultural Science 122: 79–82. https://doi.org/10.21273/JASHS.122.1.79

  48. Townsend, C. & Guest, G. (1985) Flora of Iraq, 8. Ministry of Agriculture & Agrarian Reform, Baghdad, pp. 128.

  49. Trifonova, A.A., Filyushin, M.A., Kochieva, E.Z. & Kudryavtsev, A.M. (2016) Analysis of the ITS1/ITS2 Nuclear pacers and the secondary structure of 5.8S rRNA gene in endemic species Bellevalia sarmatica (Pall. ex Georgi) Woronow and Related Species of the Subfamily Scilloideae. Russian Journal of Genetics 52 (5): 530–534.  https://doi.org/10.1134/S1022795416050112

  50. Turrill, W.B. (1929) Bellevalia tabriziana. Bulletin of Miscellaneous Information (Royal Botanic Gardens, Kew). 234 pp. https://doi.org/10.2307/4113537

  51. Wendelbo, P. (1980) Notes on Hyacinthus and Bellevalia (Liliaceae) in Turkey and Iran. Notes of the Royal Botanical Garden of Edinburgh 38 (3): 423–434

  52. Wendelbo, P. (1990) Bellevalia Lapeyr. In: Rechinger, K.H., Browicz, K., Persson, K. & Wendelbo, P. (Eds.) Flora Iranica 165. Akademische Druck-und Verlagsanstalt, Graz, pp. 149–165.

  53. Woronow, Y.N. (1925) Bellevalia speciosa. Travaux Du Museum Georgie 3: 113.

  54. Woronow, Y.N. (1927) Bellevalia sarmatica. Izvestiya Glavnogo Botaniceskago Sada S.S.S.R. Leningrad 26: 615–617.