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Published: 2021-07-27

Nuclear ITS/ETS sequence data indicate the membership of Senecio racemulifer, but not S. acutipinnus and S. graciliflorus, within the genus Jacobaea (Asteraceae, Senecioneae)

Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, Guangdong, China, Center of Conservation Biology, Core Botanical Gardens, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, Guangdong, China, University of Chinese Academy of Sciences, Beijing 100049, China
Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing 210014, China
Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, Guangdong, China, Center of Conservation Biology, Core Botanical Gardens, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, Guangdong, China, Key Laboratory of Digital Botanical Garden of Guangdong Province, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong 510650, China
Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, Guangdong, China, Center of Conservation Biology, Core Botanical Gardens, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, Guangdong, China
Eudicots karyology molecular systematics new record new synonymy taxonomy


The sectional placement of Senecio racemulifer (Asteraceae, Senecioneae) and the generic affiliation of S. acutipinnus and S. graciliflorus have been in dispute. Senecio racemulifer was tentatively considered a member of S. sect. Doria, and S. acutipinnus and S. graciliflorus were transferred to the genus Jacobaea as J. acutipinna and J. graciliflora, respectively. Our phylogenetic analyses based on nuclear ITS/ETS sequences indicate that S. racemulifer is deeply nested with Jacobaea species in a well-supported clade while S. acutipinnus and S. graciliflorus are nested with other Senecio species in a well-supported clade. We therefore transfer S. racemulifer to Jacobaea as J. racemulifera but retain S. acutipinnus and S. graciliflorus within Senecio. The names Jacobaea acutipinna, J. graciliflora, J. sect. Graciliflorae and S. ser. Racemuliferae are all synonymized. Karyological characters are reported for the three species. Jacobaea racemulifera, earlier known from the Western Tian-Shan (Kazakhstan, Kyrgyzstan, Uzbekistan), is reported here as new to China.


  1. Baldwin, B.G. & Markos, S. (1998) Phylogenetic utility of the external transcribed spacer (ETS) of 18S-26S rDNA: congruence of ETS and ITS trees of Calycadenia (Compositae). Molecular Phylogenetics and Evolution 10: 449–463.
    Boissier, E. (1844) Diagnoses Plantarum Orientalium Novarum, vol. 1(4). B. Hermann, Lipsiae, 86 pp.
    Calvo, J. & Aedo, C. (2015) A taxonomic revision of the Eurasian/northwestern African Senecio doria group (Compositae). Systematic Botany 40: 900–913.
    Calvo, J., Álvarez, I. & Aedo, C. (2015) Systematics of Senecio section Crociseris (Compositae, Senecioneae). Phytotaxa 211 (1): 1–105.
    Calvo, J., Álvarez, I., Aedo, C. & Pelser, P.B. (2013) A phylogenetic analysis and new delimitation of Senecio sect. Crociseris (Compositae, Senecioneae), with evidence of intergeneric hybridization. Taxon 62: 127–140.
    Candolle, A.P. de (1838) Prodromus Systematis Naturalis Regni Vegetabilis, vol. 6. Treuttel & Würtz, Paris, 687 pp.
    Cassini, A.H.G. de (1825) Abrotanella emarginata H. Cass. In: Cuvier, F. (Ed.) Dictionnaire des Sciences Naturelles, ed. 2, vol. 29. F.G. Levrault, Strasbourg, Paris & Le Normant, Paris, pp. 493–494.
    Chen, Y.L. (1999) Senecio L. In: Chen, Y.L. (Ed.) Flora Reipublicae Popularis Sinicae, vol. 77 (1). Science Press, Beijing, pp. 225–304.
    Chen, Y.L., Nordenstam, B. & Jeffrey, C. (2011) Senecio Linnaeus. In: Wu, Z.Y., Raven, P.H. & Hong, D.Y., (Eds.) Flora of China, vol. 20–21. Science Press, Beijing & Missouri Botanical Garden Press, St. Louis, pp. 508–536.
    Doyle, J.J. & Doyle, J.L. (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11–15.
    Dumorttier, B.C.J. (1827) Florula Belgica. J. Casterman, Tornaci Nerviorum, 172 pp.
    Fabricius, P.C. (1759) Enumeratio Methodica Plantarum. Litteris Ioannis Drimbornii, Helmstadii, 239 pp.
    Farris, J.S., Källersjö, M., Kluge, A.G. & Bult, C. (1995a) Constructing a significance test for incongruence. Systematic Biology 44: 570–572.
    Farris, J.S., Källersjö, M., Kluge, A.G. & Bult, C. (1995b) Testing significance of incongruence. Cladistics 10: 315–319.
    Felsenstein, J. (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791.
    Gagnidze, R.I. (1974) Botany and Geographical Analysis of the Floral Coenotic Complex of Subalpine Tall Herbaceous Vegetation of the Caucasus. Metsniereba, Tbilisi, 206 pp. [in Russian]
    Gaudichaud, M. (1825) Rapport sur la Flore des îles Malouines. Annales des Sciences Naturelles 5: 89–110.
    Godron, D.A. (1850) Senecio L. In: Grenier, J.C.M. & Godron, D.A. (Eds.) Flore de France, vol. 2. Chez J.-B. Baillière, Paris; chez H. Baillière, London; chez de Sainte-Agathe Aîné, Besançon, pp. 110–124.
    Gaertner, J. (1791) De Fructibus et Seminibus Plantarum, vol. 2. Typis Guilielmi Henrici Schrammii, Tubingae, 520 pp.
    Gaertner, G., Meyer, B. & Scherbius, J. (1801) Oekonomisch-technische Flora der Wetterau, vol. 3 (1). Philipp Heinreich Guilhauman, Frankfurt, pp. 1–438.
    Gray, S.M. (1821) A Natural Arrangement of British Plants, vol. 2. Baldwin, Cradock and Joy, London, 824 pp.
    Hall, T.A. (1999) BioEdit: a user friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 45: 95–98.
    Hallier, E. & Wohlfarth, R. (1897) Senecio L. In: Koch, W.D.J. (Ed.) Synopsis der Deutschen und Schweizer Flora, 3rd Ed., vol. 2. O.R. Reisland, Leipzig, pp. 1466–1499.
    Handel-Mazzetti, H. (1936) Symbolae Sinicae, vol. 7(4). Julius Springer, Wien, pp. 731–1186.
    Hillis, D.M. & Bull, J.J. (1993) An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Systematic Biology 42: 182–192.
    Hooker, J.D. (1881) Senecio Linn. In: Hooker, J.D. (Ed.) Flora of British India, vol. 3. L. Reeve & Co., London, pp. 338–356.
    Huelsenbeck, J.P., Larget, B. & Alfaro, M.E. (2004) Bayesian phylogenetic model selection using reversible jump Markov chain Monte Carlo. Molecular Biology and Evolution 21: 1123–1133.
    Jeffrey, C. & Chen, Y.L. (1984) Taxonomic studies on the tribe Senecioneae (Compositae) of eastern Asia. Kew Bulletin 39: 205–446.
    Larkin, M.A., Blackshields, G., Brown, N.P., Chenna, R., McGettigan, P.A., McWilliam, H., Valentin, F., Wallace, I.M., Wilm, A., Lopez, R., Thompson, J.D., Gibson, T.J. & Higgins, D.G. (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23: 2947–2948.
    Lessing, C.F. (1831) De plantis in expedition speculatoria Romanzoffiana observatis disserer pergunt. Synanthereae. Linnaea 6: 209–260.
    Levan, A., Fredga, K. & Sandberg, A.A. (1964) Nomenclature for centromeric position on chromosomes. Hereditas 52: 201–220.
    Liew, C.S., Memory, A.E., Barrientos, D.O., de Lange, P.J. & Pelser, P.B. (2018) The delimitation and evolutionary history of the Australasian Lautusoid group of Senecio (Asteraceae: Senecioneae). Taxon 67: 130–148.
    Linnaeus, C. (1753) Species Plantarum. Laurentius Salvius, Holmiae, 1200 pp.
    Markos, S. & Baldwin, B.G. (2001) Higher-level relationships and major lineages of Lessingia (Compositae, Astereae) based on nuclear rDNA internal and external transcribed spacer (ITS and ETS) sequences. Systematic Botany 26: 168–183.
    Mathur, R. (1995) Senecio L. In: Hajra, P.K., Rao, R.R., Singh, D.K. & Uniyal, B.P. (Eds.) Flora of India, vol. 13. Botanical Survey of India, Calcutta, pp. 238–284.
    Miller, P. (1754) The Gardeners Dictionary, abridged ed. 4. John and James Rivington, London, without pagination.
    Pavlov, N.V. (1950) New plants of Kazakhstan flora, III. Vestnik Akademii Nauk Kazakhskoi SSR. Alma Ata, Kazakh S.S.R. 8: 16–32.
    Pelser, P., Nordenstam, B., Kadereit, J. & Watson, L. (2007) An ITS phylogeny of tribe Senecioneae (Asteraceae) and a new delimitation of Senecio L. Taxon 56: 1077–1104.
    Pelser, P.B., Gravendeel, B. & van der Meijden, R. (2002) Tackling speciose genera: Species composition and phylogenetic position of Senecio sect. Jacobaea (Asteraceae) based on plastid and nrDNA sequences. American Journal of Botany 89: 929–939.
    Pelser, P.B., Gravendeel, B. & van der Meijden, R. (2003) Phylogeny reconstruction in the gap between too little and too much divergence: The closest relatives of Senecio jacobaea (Asteraceae) according to DNA sequences and AFLPs. Molecular Phylogenetics and Evolution 29: 613–628.
    Pelser, P.B., Kennedy, A.H., Tepe, E.J., Shidler, J.B., Nordenstam, B., Kadereit, J.W. & Watson, L.E. (2010) Patterns and causes of incongruence between plastid and nuclear Senecioneae (Asteraceae) phylogenies. American Journal of Botany 97: 856–873.
    Pelser, P.B., Veldkamp, J.F. & Meijden, R.V.D. (2006) New combinations in Jacobaea Mill. (Asteraceae-Senecioneae). Compositae Newsletter 44: 1–11.
    Rambaut, A., Suchard, M.A., Xie, D. & Drummond, A.J. (2014) Tracer v1.6. []
    Reichenbach, H.G.L. (1831–1832) Flora Germanica Excursoria, vol. 1(3). Carolum Cnobloch, Leipzig, pp. 185–434.
    Ren, C., Hong, Y., Wang, L. & Yang, Q.E. (2017) Generic recircumscription of Parasenecio (Asteraceae: Senecioneae) based on nuclear ribosomal and plastid DNA sequences, with descriptions of two new genera. Botanical Journal of the Linnean Society 184: 418–443.
    Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61: 539–542.
    Schischkin, B. (1961) Senecio L. Cass. In: Schischkin, B. & Bobrov, E.G. (Eds.) Flora SSSR, vol. 26. Akademiya Nauk SSSR Publisher, Moscow-Leningrad, pp. 699–788. [in Russian]
    Sennikov, A.N. & Averyanov, L.V. (2008) Senecio acutipinnus, a rare creeping Chinese plant now discovered in Vietnam, and its taxonomic position in Senecioneae (Asteraceae). Komarovia 6: 92–99.
    Stamatakis, A. (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30: 1312–1313.
    Stamatakis, A., Hoover, P. & Rougemont, J. (2008) A rapid bootstrap algorithm for the RAxML Web servers. Systematic Biology 57: 758–771.
    Swofford, D.L. (2003) PAUP*: phylogenetic analysis using parsimony (*and other methods), version 4.0b10. Sinauer, Sunderland.
    Tojibaev, K., Jang, C.G., Lazkov, G., Chang, K.S., Sitpayeva, G.T., Safarov, N., Beshko, N.Y., Muktubaeyeva, S.K, Vesselova, P.V., Turakulov, I., Abdurakhmanova, Y.Y., Na, N., Park, M.-S., Choi, K., Choi, H.-J., Oh, B.-U. & Oh, S.-H. (2020) An annotated checklist of endemic vascular plants of the Tian-Shan Mountains in central Asian countries. Phytotaxa 464: 117–158.
    Turland, N.J., Wiersema, J.H., Barrie, F.R., Greuter, W., Hawksworth, D.L., Herendeen, P.S., Knapp, S., Kusber, W.-H., Li, D.-Z., Marhold, K., May, T.W., McNeill, J., Monro, A.M., Prado, J., Price, M.J. & Smith, G.F. (2018) International Code of Nomenclature for algae, fungi, and plants (Shenzhen Code) adopted by the Nineteenth International Botanical Congress Shenzhen, China, July 2017. Regnum Vegetabile 159. Koeltz Botanical Books, Glashütten, 254 pp.
    Wiebe, E.I. (2000) The annotated check-list of the tribe Senecioneae Cass. (Asteraceae) in Siberia. Turczaninowia 3: 58–63.
    White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, pp. 315–322.