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Type: Article
Published: 2020-11-17
Page range: 234–246
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Notholirion campanulatum is co-specific with N. bulbuliferum (Liliaceae) based on morphology and molecular data

College of Life Science, Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, Sichuan University, Chengdu 610064, PR China
College of Life Science, Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, Sichuan University, Chengdu 610064, PR China
College of Life Science, Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, Sichuan University, Chengdu 610064, PR China
College of Life Science, Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, Sichuan University, Chengdu 610064, PR China
College of Life Science, Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, Sichuan University, Chengdu 610064, PR China
Monocots cpDNA sequences Liliaceae morphology synonym taxonomy

Abstract

Notholirion campanulatum and N. bulbuliferum are parapatrically distributed in the Qinghai-Tibetan Plateau and adjacent regions, however, their relationship has been controversial for a long time. In this study, six morphological characters including colour of corolla/ovary/bulblet, corolla shape, tepal size, and pollen form, together with nuclear ITS and four cpDNA markers (matK, ndhA, ndhG-I, and petL-G) were selected to perform a comprehensive analysis on 23 populations. Our results indicate that morphological characters of N. campanulatum and N. bulbuliferum are congruent with each other, and the phylogenetic analysis revealed that N. campanulatum closely nested with N. bulbuliferum. Therefore, we propose that these two species should be regarded as synonyms.

References

<p class="Reference">Altýnordu, F., Peruzzi, L., Yu, Y. &amp; He, X.J. (2016) A tool for the analysis of chromosomes: KaryoType. <em>Taxon </em>65: 586–592.</p><p class="Reference">         <a href="https://doi.org/10.12705/653.9">https://doi.org/10.12705/653.9</a></p><p class="Reference">Cotton, A.D. &amp; Stearn, W.T. (1934) <em>The Liliy Year Book </em>3. Royal Horticultural Society, London, 19 pp.</p><p class="Reference">Coyne, J.A. &amp; Orr, A. (1998) The evolutionary genetics of speciation.<em> Philosophical Transactions of the Royal Society. Biological sciences</em> 353: 287–305.</p><p class="Reference">         <a href="https://doi.org/10.1098/rstb.1998.0210">https://doi.org/10.1098/rstb.1998.0210</a></p><p class="Reference">Darwin, C. (1859) <em>On the origin of species by means of natural selection or the presentation of favoured races in the struggle for life.</em> John Murray, London, 462 pp.</p><p class="Reference">Doyle, J.J. &amp; Doyle, J.L. (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. <em>Phytochemical Bulletin</em> 19: 11–15.</p><p class="Reference">Fennessy, J., Bidon, T., Reuss, F., Kumar, V., Elkan, P., Maria, A.N., Vamberger, M., Fritz, W. &amp; Janke, A. (2016) Multi-locus analyses reveal four giraffe species instead of one.<em> Current Biology </em>26: 1–7.</p><p class="Reference">         <a href="http://dx.doi.org/10.1016/j.cub.2016.07.036">http://dx.doi.org/10.1016/j.cub.2016.07.036</a></p><p class="Reference">Frazão, A. &amp; Lohmann, L.G. (2020) Taxonomic placement of <em>Tanaecium mutabile</em> (Bignonieae, Bignoniaceae) based on new morphological data and phylogenetic analyses. <em>Phytotaxa</em> 438 (5): 289–300.</p><p class="Reference">         <a href="https://doi.org/10.11646/phytotaxa.438.5.2">https://doi.org/10.11646/phytotaxa.438.5.2</a></p><p class="Reference">Gao, Y.D. (2015) Continuous variation supports accommodating <em>Lilium habaense</em> and <em>L. xanthellum</em> within <em>L. stewartianum</em> (Liliaceae). <em>Phytotaxa</em> 226 (2): 196–198.</p><p class="Reference">         <a href="https://doi.org/10.11646/phytotaxa.226.2.10">https://doi.org/10.11646/phytotaxa.226.2.10</a></p><p class="Reference">Gao, Y.D., Harris, A.J., Zhou, S.D. &amp; He, X.J. (2013) Evolutionary events in <em>Lilium</em> (including <em>Nomocharis</em>, Liliaceae) are temporally correlated with orogenies of the Q-T plateau and the Hengduan Mountains. <em>Molecular Phylogenetics and Evolution</em> 68: 443–460.</p><p class="Reference">         <a href="http://dx.doi.org/10.1016/j.ympev.2013.04.026">http://dx.doi.org/10.1016/j.ympev.2013.04.026</a></p><p class="Reference">Grant, V. (1981) <em>Plant Speciation</em>. Columbia University Press, New York.</p><p class="Reference">Huang, J., Yang, L.Q., Yu, Y., Liu, Y.M., Xie, D.F., Li, J., Zhou, S.D. &amp; He, X.J. (2018) Molecular phylogenetics and historical biogeography of the tribe Lilieae (Liliaceae): bi-directional dispersal between biodiversity hotspots in Eurasia. <em>Annals of Botany</em> 122: 1245–1262.</p><p class="Reference">         <a href="https://doi.org/10.1093/aob/mcy138">https://doi.org/10.1093/aob/mcy138</a></p><p class="Reference">Lee, S.Y., Choi, K.S., Choi, K.H., Yoon, T.M., &amp; Jung, H.Y. (2013) Morphological differences between larvae of the oriental fruit moth (<em>Grapholita molesta</em> Busck) and the peach fruit moth (<em>Carposina sasakii</em> Matsumura) in korea. <em>Advanced Synthesis &amp; Catalysis</em> 43: 21–26.</p><p class="Reference">         <a href="https://doi.org/10.9729/AM.2013.43.1.21">https://doi.org/10.9729/AM.2013.43.1.21</a></p><p class="Reference">Leigh, J. &amp; Bryant, D. (2015) POPART: full-feature software for haplotype network construction. <em>Methods in Ecology and Evolution</em> 6: 11.</p><p class="Reference">         <a href="https://doi.org/10.1111/2041-210X.12410">https://doi.org/10.1111/2041-210X.12410</a></p><p class="Reference">Li, J., Liu, H.Y., Xie, D.F., Zhou, S.D. &amp; He, X.J. (2018) The complete chloroplast genome of <em>Notholition macrophyllum</em>. <em>Mitochondrial DNA Part B Resources</em> 3: 1102–1103.</p><p class="Reference">         <a href="https://doi.org/10.1080/23802359.2018.1463825">https://doi.org/10.1080/23802359.2018.1463825</a></p><p class="Reference">Li, S.F., Li, B. &amp; Qi, H. (2003) Study on cytology of some species in Liliaceae from Qinling mountain. <em>Acta Botanica Boreali-Occidentalia Sinica</em> 23: 1949–1955.</p><p class="Reference">Liang, S.Y. (1995) Chorology of Liliaceae (s.str.) and its bearing on the Chinese flora. <em>Acta Phytotaxonomica Sinica</em> 33: 27–51.</p><p class="Reference">Liang, S.Y. &amp; Tamura, M.N. (2000)<em> Notholirion</em> Wallich ex Boissier.<em> In: </em>Wu, Z.Y. &amp; Raven, P.H. (Eds.) <em>Flora of China </em>24. Science Press, Beijing &amp; Missouri Botanical garden Press, Saint Louis, pp. 133–134.</p><p class="Reference">Lingelsheim, A. (1922) <em>Repertorium Specierum Novarum Regni Vegetabilis </em>12. Selbstverlag des Herausgebers, Berlin, 316 pp.</p><p class="Reference">Peruzzi, L. (2016) A new infrafamilial taxonomic setting for Liliaceae, with a key to genera and tribes. <em>Plant Biosystems </em>150: 1341–1347.</p><p class="Reference">         <a href="https://doi.org/10.1080/11263504.2015.1115435">https://doi.org/10.1080/11263504.2015.1115435</a></p><p class="Reference">Mallet, J. (1995) A species definition for the modern synthesis.<em> Trends in Ecology &amp; Evolution</em> 10: 294–299.</p><p class="Reference">         <a href="https://doi.org/10.1016/0169-5347(95)90031-4">https://doi.org/10.1016/0169-5347(95)90031-4</a></p><p class="Reference">Marousky, F. J. (1967) <em>Effects of temperature, light and nutrition on color and anthocyanin content of poinsettia bracts.</em> Virginia Polytechnic Institute, Virginia, 114 pp.</p><p class="Reference">Noltie, H.J. (1994) <em>Flora of Bhutan </em>3(1). Royal Botanic Gardens, Edinburgh, 456 pp.</p><p class="Reference">Nooteboom, H.P. (1992) A point of view on the species concept. <em>Taxon</em> 41: 318–320.</p><p class="Reference">         <a href="https://doi.org/10.2307/1222338">https://doi.org/10.2307/1222338</a></p><p class="Reference">Nylander, J.A.A. (2004) <em>MrModelTest 2.2. Program Distributed by the Author</em>. Evolutionary Biology Centre, Uppsala University. Available from: <a href="http://www.abc.se/~nylander/">http://www.abc.se/~nylander/</a> (accessed 5 October 2020)</p><p class="Reference">Rambaut, A., Suchard, M.A., Xie, D. &amp; Drummond, A.J. (2013) <em>Tracer v1.6.0</em>. Available from: <a href="http://beast.bio.ed.ac.uk">http://beast.bio.ed.ac.uk</a> (accessed 5 October 2020)</p><p class="Reference">Ronquist, F., Teslenko, M., Mark, P., Ayres, D.L., Darling, A., Hohna, S., Larget, B., Liu, L., Suchard, M.A. &amp; Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. <em>Systematic Biology</em> 61: 539–542.</p><p class="Reference">         <a href="https://doi.org/10.1093/sysbio/sys029">https://doi.org/10.1093/sysbio/sys029</a></p><p class="Reference">Ronsted, N., Law, S., Thornton, H., Fay, M.F. &amp; Chase, M.W. (2005) Molecular phylogenetic evidence for the monophyly of <em>Fritillaria</em> and <em>Lilium</em> (Liliaceae; Liliales) and the infrageneric classification of <em>Fritillaria</em>. <em>Molecular Phylogenetics and Evolution</em> 35: 509–527.</p><p class="Reference">         <a href="https://doi.org/10.1016/j.ympev.2004.12.023">https://doi.org/10.1016/j.ympev.2004.12.023</a></p><p class="Reference">Shipunov, A. (2015) <em>Plantago schrenkii</em> is <em>P. maritima</em>: morphological and molecular evidence. <em>Annales Botanici Fennici</em> 52: 33–37.</p><p class="Reference">         <a href="https://doi.org/10.5735/085.052.0205">https://doi.org/10.5735/085.052.0205</a></p><p class="Reference">Singh, N.P. &amp; Sanjappa, M. (2006) Alliaceae, Liliaceae, Trilliaceae &amp; Uvulariaceae. <em>Fascicles of Flora of India</em> 23. Botanical Survey of India, New Delhi, 134 pp.<em></em></p><p class="Reference">Stapf, O. (1934) <em>Bulletin of Miscellaneous Information </em>2. H.M. Stationery Office, London, 96 pp.</p><p class="Reference">         <a href="https://doi.org/10.2307/4118229">https://doi.org/10.2307/4118229</a></p><p class="Reference">Stearn (1950)<em> Kew Bulletin </em>5(3). Royal Botanic Gardens Kew Press, London, 421 pp.</p><p class="Reference">Sudhir, K., Glen, S., Michael, L., Christina, K. &amp; Koichiro, T. (2018) MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. <em>Molecular Biology and Evolution</em> 35: 1547–1549.</p><p class="Reference">         <a href="https://doi.org/10.1093/molbev/msy096">https://doi.org/10.1093/molbev/msy096</a></p><p class="Reference">Tan, Y.H., Chen, Y.J. &amp; Li, B. (2016) New synonyms for <em>Premna yunnanensis</em> (Lamiaceae) in China. <em>Phytotaxa</em> 283 (2): 196–200.</p><p class="Reference">         <a href="https://doi.org/10.11646/phytotaxa.283.2.10">https://doi.org/10.11646/phytotaxa.283.2.10</a></p><p class="Reference">Wallich, N. &amp; Boissier, P.E. (1882) <em>Flora Orientalis </em>5. NCSU Libraries, Basileae, 190 pp.</p><p class="Reference">White, T.J., Bruns, T., Lee, S. &amp; Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. <em>In:</em> Innis, M.A., Gelfand, D.H., Sninsky, J.J. &amp; White T.J. (Ed.) <em>PCR protocols: A guide to methods and applications</em>. San Diego, Academic, pp. 315–322.</p><p class="Reference">         <a href="https://doi.org/10.1016/B978-0-12-372180-8.50042-1">https://doi.org/10.1016/B978-0-12-372180-8.50042-1</a></p><p class="Reference">Wilson, E.H. (1925) The lilies of eastern Asia: a monograph. <em>Nature</em> 116: 1–355.</p><p class="Reference">         <a href="https://doi.org/10.1038/116355a0">https://doi.org/10.1038/116355a0</a></p><p class="Reference">Xiao, Q.Y., Hu, H.Y., Tong, F., Li, M.J. &amp; He, X.J. (2017) <em>Semenovia torilifolia</em> is conspecific with <em>S. malcolmii</em> (Apiaceae) based on morphology and molecular data. <em>Phytotaxa</em> 321 (3): 225–237.</p><p class="Reference">         <a href="https://doi.org/10.11646/phytotaxa.321.3.1">https://doi.org/10.11646/phytotaxa.321.3.1</a></p><p class="Reference">Xu L.F., Xu H., Cao Y.W., Yang, P.P., Feng, Y.Y., Tang, Y.C., Yuan, S.X. &amp; Ming, J. (2017) Validation of reference genes for quantitative real-time PCR during bicolor tepal development in Asiatic hybrid Lilies (<em>Lilium</em> spp.). <em>Frontiers in Plant Science</em> 8: 669.</p><p class="Reference">         <a href="https://doi.org/10.3389/fpls.2017.00669">https://doi.org/10.3389/fpls.2017.00669</a></p><p class="Reference">Yang, M., Zhou, S.D., He, X.J. &amp; Peng, C. (2016) Genetic relationships of <em>Notholirion</em> and genetic diversity of <em>N. bulbuliferum</em> in China revealed by ISSR markers. <em>Acta Botanica Boreali-Occidentalia Sinica</em> 36: 449–457.</p><p class="Reference">Yassin, A., Capy, P., Madi, R.L., Ogereau, D. &amp; David, J.R. (2008) DNA barcode discovers two cryptic species and two geographical radiations in the invasive drosophilid <em>Zaprionus indianus</em>.<em> Molecular Ecology Resources</em> 8: 491–501.</p><p class="Reference">         <a href="https://doi.org/10.1111/j.1471-8286.2007.02020.x">https://doi.org/10.1111/j.1471-8286.2007.02020.x</a></p><p>Yu, H., Huang, R.F. &amp; Zang, Y.J. (1996) karyotypical study of <em>Notholirion bulbuliferum</em> in Yunnan. <em>Acta Botanica Yunnanica Supplement</em> 8: 59–77.</p>