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Published: 2018-08-30

Retiboletus nigrogriseus and Tengioboletus fujianensis, two new boletes from the south of China

College of Materials and Chemistry Engineering, Hainan University, Haikou 570228, China College of Pharmacy-Transgenic Laboratory, Hainan Medical University, Haikou 571199, China
College of Pharmacy-Transgenic Laboratory, Hainan Medical University, Haikou 571199, China
Hainan Yinggeling National Nature Reserve, Baisha, 572800, China
College of Pharmacy-Transgenic Laboratory, Hainan Medical University, Haikou 571199, China
College of Pharmacy-Transgenic Laboratory, Hainan Medical University, Haikou 571199, China
College of Pharmacy-Transgenic Laboratory, Hainan Medical University, Haikou 571199, China
College of Materials and Chemistry Engineering, Hainan University, Haikou 570228, China
Fungi new taxa molecular phylogeny morphology taxonomy


Two new boletes, viz. Retiboletus nigrogriseus and Tengioboletus fujianensis are described based on morphological and molecular evidence. Retiboletus nigrogriseus is characterized by a black pileus when young, gray when old, context and hymenophore staining brownish to fuliginous when injured, black, reticulate stipe with a white basal mycelium, and a subcutis pileipellis composed of uninflated hyphae with cystidioid terminal cells; T. fujianensis is characterized by a large-sized basidioma, brown to reddish brown pileus, reticulate stipe, and a trichodermal pileipellis composed of uninflated hyphae with cystidioid terminal cells. A detailed description, color photos of fresh basidiomata and line drawings of microstructures of the two new boletes are presented.


  1. Binder, M. & Bresinsky, A. (2002) Retiboletus, a new genus for a species complex in the Boletaceae producing retipolides. Feddes Repertorium 113: 30–40.<30::AID-FEDR30>3.0.CO;2-D

    Chai, H., Liang, Z.Q., Jiang, S., Fu, X.L. & Zeng, N.K. (2018) Lanmaoa rubriceps, a new bolete from tropical China. Phytotaxa 347 (1): 71–80.

    Chen, Z.H., Yang, Z.L., Tolgor, B. & Li, T.H. (2016) Poisonous mushrooms: recognition and poisoning treatment. Science Press, Beijing, 308 pp.

    Corner, E.J.H. (1972) Boletus in Malaysia. Botanic Gardens, Singapore, 263 pp.

    Desjardin, D.E., Binder, M., Roekring, S. & Flegel, T. (2009) Spongiforma, a new genus of gasteroid boletes from Thailand. Fungal Diversity 37: 1–8.

    Desjardin, D.E., Peay, K.G. & Bruns, T.D. (2011) Spongiforma squarepantsii, a new species of gasteroid bolete from Borneo. Mycologia 103: 1119–1123.

    Endo, M. & Hongo, T. (1976) Materials for the fungus flora of Japan 24. Transactions of the Mycological Society of Japan 17: 345–348.

    Feng, B., Xu, J., Wu, G., Zeng, N.K., Li, Y.C., Tolgor, B., Kost, G.W. & Yang, Z.L. (2012) DNA sequence analyses reveal abundant diversity, endemism and evidence for Asian origin of the porcini mushrooms. PLoS One 7: e37567.

    Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.

    Halling, R.E., Nuhn, M., Osmundson, T.D., Fechner, N.A., Trappe, J.M., Soytong, K., Arora, D., Hibbett, D.S. & Binder, M. (2012) Affinities of the Boletus chromapes group to Royoungia and the description of two new genera, Harrya and Australopilus. Australian Systematic Botany 25: 418–431.

    Heim, R. (1963) Diagnoses latines des espèces de champignons, ou nonda, associés à la folie du komugl tai et dundaal. Revue de Mycologie 28: 277–283.

    Horak, E. (2011) Revision of Malaysian species of Boletales s.l. (Basidiomycota) described by E.J.H. Corner (1972, 1974). Malayan Forest Records 51: 1–283.

    Hosen, M.I., Feng, B., Wu, G., Zhu, X.T., Li, Y.C. & Yang, Z.L. (2013) Borofutus, a new genus of Boletaceae from tropical Asia: morphology, taxonomy and phylogeny. Fungal Diversity 58: 215–226.

    Huelsenbeck, J.P. & Ronquist, F. (2005) Bayesian analysis of molecular evolution using MrBayes. In: Nielsen, R. (Eds.) Statistical methods in molecular evolution. Springer, New York, pp. 183–226.

    James, T.Y., Kauff, F., Schoch, C., Matheny, P.B., Hofstetter, V., Cox, C., Celio, G., Gueidan, C., Fraker, E., Miadlikowska, J., Lumbsch, H.T., Rauhut, A., Reeb, V., Arnold, A.E., Amtoft, A., Stajich, J.E., Hosaka, K., Sung, G.H., Johnson, D., O’Rourke, B., Crockett, M., Binder, M., Curtis, J.M., Slot, J.C., Wang, Z., Wilson, A.W., Schüßler, A., Longcore, J.E., O’Donnell, K., Mozley-Standridge, S., Porter, D., Letcher, P.M., Powell, M.J., Taylor, J.W., White, M.M., Griffith, G.W., Davies, D.R., Humber, R.A., Morton, J.B., Sugiyama, J., Rossman, A.Y., Rogers, J.D., Pfister, D.H., Hewitt, D., Hansen, K., Hambleton, S., Shoemaker, R.A., Kohlmeyer, J., Volkmann-Kohlmeyer, B., Spotts, R.A., Serdani, M., Crous, P.W., Hughes, K.W., Matsuura, K., Langer, E., Langer, G., Untereiner, W.A., Lücking, R., Büdel, B., Geiser, D.M., Aptroot, A., Diederich, P., Schmitt, I., Schultz, M., Yahr, R., Hibbett, D., Lutzoni, F., McLaughlin, D., Spatafora, J. & Vilgalys, R. (2006) Reconstructing the early evolution of the fungi using a six gene phylogeny. Nature 443: 818–822.

    Katoh, K., Kuma, K., Toh, H. & Miyata, T. (2005) MAFFT version 5: improvement in accuracy of multiple sequence alignment. Nucleic Acids Research 33: 511–518.

    Kornerup, A. & Wanscher, J.H. (1981) Taschenlexikon der Farben. 3. Aufl. Muster-Schmidt Verlag, Göttingen, 242 pp.

    Liang, Z.Q., Chai, H., Jiang, S., Ye, Z.K. & Zeng, N.K. (2017) The genus Xanthoconium (Boletaceae, Boletales) in tropical China. Phytotaxa 295 (3): 246–254.

    Mao, X.L. (1998) Economic fungi of China. Science Press, Beijing, 762 pp.

    Mikheyev, A.S., Mueller, U.G. & Abbot, P. (2006) Cryptic sex and many-to-one coevolution in the fungus-growing ant symbiosis. Proceedings of the National Academy of Sciences of the United States of America 103: 10702–10706.

    Nylander, J.A.A. (2004) MrModeltest 2.3. Program distributed by the author. Evolutionary Biology Center, Uppsala University.

    Raspé, O., Vadthanarat, S., Kesel, A.D., Degreef, J., Hyde, K.D. & Lumyong, S. (2016) Pulveroboletus fragrans, a new Boletaceae species from northern Thailand, with a remarkable aromatic odor. Mycological Progress 15: 38.

    Roman, M.D., Claveria, V. & Miguel, A.M.D. (2005) A revision of the descriptions of ectomycorrhizas published since 1961. Mycological Research 109: 1063–1104.

    Smith, S.A. & Dunn, C.W. (2008) Phyutility: a phyloinformatics tool for trees, alignments and molecular data. Bioinformation 24: 715–716.

    Stamatakis, A. (2006) RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22: 2688–2690.

    Swofford, D.L. (2002) PAUP*: phylogenetic analysis using parsimony (*and other methods), version 4.0b10. Sinauer Associates, Sunderland, 2000 pp.

    Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4238–4246.

    White, T.J., Bruns, T., Lee, S. & Taylor, J.W. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenies. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR protocols: a guide to methods and applications. Academic Press, New York, pp. 315–322.

    Wu, G., Feng, B., Xu, J., Zhu, X.T., Li, Y.C., Zeng, N.K., Hosen, M.I. & Yang, Z.L. (2014) Molecular phylogenetic analyses redefine seven major clades and reveal 22 new generic clades in the fungal family Boletaceae. Fungal Diversity 69: 93–115.

    Wu, G., Li, Y.C., Zhu, X.T., Zhao, K., Han, L.H., Cui, Y.Y., Li, F., Xu, J.P. & Yang, Z.L. (2016) One hundred noteworthy boletes from China. Fungal Diversity 81: 25–188.

    Wu, X.L., Mao, X.L., Tolgor, B., Song, B., Li, T.H., Zhao, Y.X., Chen, S.L., Zeng, N.K., Huang, S.Z., Wen, T.C. & Deng, C.Y. (2013) Medicinal fungi of China. Science Press, Beijing, 923 pp.

    Zang, M. (2006) Flora fungorum sinicorum. Vol. 22. Boletaceae I. Science Press, Beijing, 215 pp.

    Zeng, N.K., Liang, Z.Q., Tang, L.P., Li, Y.C. & Yang, Z.L. (2017) The genus Pulveroboletus (Boletaceae, Boletales) in China. Mycologia 109: 422–442.

    Zeng, N.K., Liang, Z.Q., Wu, G., Li, Y.C. & Yang, Z.L. (2016) The genus Retiboletus in China. Mycologia 108: 363–380.

    Zeng, N.K., Tang, L.P., Li, Y.C., Tolgor, B., Zhu, X.T., Zhao, Q. & Yang, Z.L. (2013) The genus Phylloporus (Boletaceae, Boletales) from China: morphological and multilocus DNA sequence analyses. Fungal Diversity 58: 73–101.

    Zeng, N.K., Wu, G., Li, Y.C., Liang, Z.Q. & Yang, Z.L. (2014) Crocinoboletus, a new genus of Boletaceae (Boletales) with unusual boletocrocin polyene pigments. Phytotaxa 175 (3): 133–140.