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Published: 2018-08-30

Polyphasic characterization of newly isolated Anabaenopsis (Cyanobacteria) strains from tropical Brazil and Mexico

Centro Universitário Leonardo da Vinci-Uniasselvi, Rua Doutor Pedrinho, 77, 89082-262, Indaial, SC, Brazil
Universidade Luterana do Brasil, Unidade Universitária de Cachoeira do Sul, Rua Martinho Lutero 301, 96501-595, Cachoeira do Sul, RS, Brazil
University of São Paulo, Center of Nuclear Energy in Agriculture, Avenida Centenário 303, 13416-000, Piracicaba, SP, Brazil
Fort Lauderdale Research and Education Center, University of Florida-IFAS, 3205 College Avenue, Davie, FL 33314, USA
Norwegian Institute of Water Research (NIVA) Gaustadalléen 21 0349 Oslo, Norway
Facultad de Ciencias, Universidad Nacional Autónoma de México. Circuito exterior s/n, Coyoacán, México D.F. 04510, México


University of São Paulo, Center of Nuclear Energy in Agriculture, Avenida Centenário 303, 13416-000, Piracicaba, SP, Brazil


Institute of Botany, Nucleus of Phycology, Avenida Miguel Estéfano 3687, 04301-012, São Paulo, SP, Brazil
Algae Biodiversity Cyanobacterial bloom Extremophiles Shallow lakes Taxonomy


The taxonomy of four isolated strains of Anabaenopis elenkinii and A. arnoldii from North and South America were investigated using a polyphasic approach. The studied populations were collected from seven alkaline lakes of the Brazilian Pantanal and Nabor Carrillo Lake, Texcoco region (Mexico), between 2004 and 2012, where they frequently formed blooms during the dry season. The morphological plasticity of the populations, from natural and culture conditions, was described based on 200 individuals of each species. The 16S rRNA and the cpc-BA-IGS phylogenetic analyses show Anabaenopsis as a sister-taxon of Cyanospira. The Anabaenopsis clade has three strongly supported subclusters related to the origin of strains: Pantanal+Mexico, Kenya+Australia and one European clade. Although these data indicate that the currently known Anabaenopsis strains belong to more than one species, we are being conservative and not describing them as new species due to the lack of more molecular data. We highlight the need of a revision of the genus, considering also that Anabaenopsis sp. PCC 9215, the current reference strain used for the genus, is not identified as A. elenkinii and it is phylogenetically grouped with Anabaenopsis strains identified as A. circularis, A. arnoldii, A. nadsonii and A. elenkinii.


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