We describe a new species of Tuber from Yunnan Province, China, based on molecular and morphological data. This species is characterized by its snow-white ascomata, pubescent peridia and subglobose or globose ascospores. Tuber sinoniveum sp. nov. is phylogenetically closely related to T. sinosphaerosporum but differs in having ascospores with more meshes (3–9 along) and glabrous peridia. Phylogenetic placement of this novel Tuber species is in the Puberulum group.