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A new species of Leptolalax (Anura: Megophryidae) from Son Tra Peninsula, central Vietnam



Morphological, acoustic and molecular analyses result in the description of Leptolalax rowleyae sp. nov., a new species of frog in the Megophryidae, belonging to the L. applebyi Rowley & Cao species group from central Vietnam. It differs from its congeners by a combination of the following morphological attributes: (1) adult SVL 23.4–25.4 mm in males and 27–27.8 mm in females; (2) presence of distinct dark/brown dorsolateral markings, including black spots on flanks; (3) pinkish milk-white to light brown chest and belly with numerous white speckles; (4) tympanum distinct; (5) absence of webbing or lateral dermal fringes on fingers and toes; (6) pectoral glands comparatively small (3.3–4.7% of SVL); (7) ventrolateral glands indistinct; and (8) iris bicolored with copper tint in upper half fading to golden in lower third of iris. The male advertisement call of the new species consists of 4–6 notes, lacking a distinct introductory note, with an average dominant frequency of 3.2–3.5 kHz. The description of the tadpole constitutes the first description of larval morphology for a member of the L. appleybi species group. Genetically, an uncorrected sequence divergence of 7.4% for 16S rRNA separates the new species from its two closest relatives, L. ardens and L. melicus in the L. applebyi species group.



Amphibia, Acoustics, Leptolalax applebyi species group, tadpole morphology, Son Tra Nature Reserve

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Altig, R. (2007) A primer for the morphology of anuran tadpoles. Herpetological Conservation and Biology, 2 (1), 71–74.

Altig, R. & McDiarmid, R.W. (1999) Body plan: development and morphology. In: McDiarmid, R.W. & Altig, R. (Eds.), Tadpoles: The Biology of Anuran Larvae. University of Chicago Press, Chicago, pp. 24–51.

Altig, R., Lathrop, A. & Murphy, R.W. (2009) Morphology of Southeast Asian tadpoles: Hoplobatrachus chinensis (Dicroglossidae), Leptolalax pelodytoides (Megophryidae), and other megophryids. Russian Journal of Herpetology, 16 (2), 126–130.

Boulenger, G.A. (1893) Concluding report on the reptiles and batrachians obtained in Burma by Signor L. Fea dealing with the collection made in Pegu and the Karin Hills in 1887–88. In: Annali del Museo Civico di Storia Naturale di Genova, 1893, pp. 304–347.

Boulenger, G.A. (1900) Descriptions of new batrachians and reptiles from the Larut Hills, Perak. In: Hornell, J. (Ed.), The Annals and magazine of natural history; zoology, botany, and geology being a continuation of the Annals combined with Loudon and Charlesworth's Magazine of Natural History. Taylor and Francis, Ltd., London, pp. 186–193.

Dehling, J.M. (2012) Eine neue Art der Gattung Leptolalax (Anura: Megophryidae) vom Gunung Benom, Westmalaysia/A new species of the genus Leptolalax (Anura: Megophryidae) from Gunung Benom, Peninsular Malaysia. Sauria, 34, 9–21.

Delorme, M., Dubois, A., Grosjean, S. & Ohler, A. (2006) Une nouvelle ergotaxinomie des Megophryidae (Amphibia, Anura). Alytes, 24, 6–21.

Dubois, A. (1980) Notes sur la systematique et la repartition des amphibiens anoures de Chine et des regions avoisinantes IV. Classification generique et subgenerique des Pelobatidae Megophryinae. Bulletin mensuel de la Société linnéenne de Lyon, 49 (8), 469−482.

Dubois, A. (1983) Note préliminaire sur le genre Leptolalax Dubois, 1980 (Amphibiens, Anoures), avec diagnose d'une espèce nouvelle du Vietnam. Alytes, 2, 147–153.

Felsenstein, J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 39 (4), 783–791.

Felsenstein, J. (2004) Inferring phylogenies. Vol. 2. Sinauer Associates, Inc. Publishers, Sunderland, Massachusetts, 465 pp.

Frost, D.R. (2017) Amphibian Species of the World: an Online Reference. Version 6.0. Electronic Database accessible. Available from: (acessed 13 July 2017)

Goloboff, P.A., Farris, J.S. & Nixon, K.C. (2008) TNT, a free program for phylogenetic analysis. Cladistics, 24 (5), 774–786.

Gosner, K.L. (1960) A simplified table for staging anuran embryos and larvae with notes on identification. Herpetologica, 16, 183–190.

Grismer, L.L., Grismer, J.L. & Youmans, T.M. (2004) A new species of Leptolalax (Anura: Megophryidae) from Pulau Tioman, West Malaysia. Asiatic Herpetological Research, 10, 08–11.

Grosjean, S. (2006) The choice of external morphological characters and developmental stages for tadpole–based anuran taxonomy: a case study in Rana (Sylvirana) nigrovittata (Blyth, 1855) (Amphibia, Anura, Ranidae). Contributions to Zoology, 74, 61–76.

Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41 (1999), 95–98.

Hedges, S.B. (1994) Molecular evidence for the origin of birds. Proceedings of the National Academy of Sciences, 91 (7), 2621–2624.

Hillis, D.M. & Bull, J.J. (1993) An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Systematic Biology, 42 (2), 182–192.

Huelsenbeck, J.P. & Hillis, D.M. (1993) Success of phylogenetic methods in the four-taxon case. Systematical Biology, 42, 247–264.

Huelsenbeck, J.P. & Ronquist, F. (2001) MRBAYES: Bayesian inference of phylogeny. Bioinformatics, 17, 754–755.

Inger, R.F., Darevsky, I.S. & Orlov, N.L. (1999) Frogs of Vietnam : a report on new collections. Fieldiana Zoology, 92, 1–46.

Jiang, K., Yan, F., Suwannapoom, C., Chomdej, S. & Che, J. (2013) A new species of the genus Leptolalax (Anura: Megophryidae) from northern Thailand. Asian Herpetological Research, 4 (2), 100–108.

Köhler, J., Jansen, M., Rodríguez, A., Kok, P., Toledo, L.F., Emmrich, M., Glaw, F., Haddad, C.F.B., Rödel, M.O. & Vences, M. (2017) The use of bioacoustics in anuran taxonomy: theory, terminology, methods and recommendations for best practice. Zootaxa, 4251 (1), 1–124.

Kumar, S., Stecher, G. & Tamura, K. (2016) MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33 (7), 1870–1874.

Lathrop, A., Murphy, R.W., Orlov, N. & Ho, C.T. (1998) Two new species of Leptolalax (Anura: Megophryidae) from northern Vietnam. Amphibia-Reptilia, 19 (3), 253–267.

Li, C., Guo, X.G. & Wang, Y.Z. (2011) Tadpole types of Chinese megophryid frogs (Anura: Megophryidae) and implications for larval evolution. Current Zoology, 57 (1), 93−100.

Matsui, M. (1997) Call characteristics of Malaysian Leptolalax with a description of two new species (Anura: Pelobatidae). Copeia, 1997 (1), 158–165.

Matsui, M. (2006) Three new species of Leptolalax from Thailand (Amphibia, Anura, Megophryidae). Zoological Science, 23 (9), 821–830.

Matsui, M., Belabut, D.M., Ahmad, N. & Yong, H.S. (2009) A new species of Leptolalax (Amphibia, Anura, Megophryidae) from Peninsular Malaysia. Zoological Science, 26 (3), 243–247.

Miller, K.G., Kominz, M.A., Browning, J.V., Wright, J.D., Mountain, G.S., Katz, M.E., Sugarman, P.J., Cramer, B.S., Christie-Blick, N. & Pekar, S.F. (2005) The Phanerozoic record of global sea-level change. Science, 310 (5752), 1293–1298.

Oberhummer, E., Barten, C., Schweizer, M., Das, I., Haas, A. & Hertwig, S.T. (2014) Description of the tadpoles of three rare species of megophryid frogs (Amphibia: Anura: Megophryidae) from Gunung Mulu, Sarawak, Malaysia. Zootaxa, 3835 (1), 59–79.

Ohler, A., Marquis, O., Grosjean, S. & Swan, S. (2000) Amphibian biodiversity of Hoang Lien Nature Reserve (Lao Cai Province, northern Vietnam) with description of two new species. Herpetozoa, 13 (1/2), 71–87.

Ohler, A., Wollenberg, K.C., Grosjean, S., Hendrix, R., Vences, M., Ziegler, T. & Dubois, A. (2011) Sorting out Lalos: description of new species and additional taxonomic data on megophryid frogs from northern Indochina (genus Leptolalax, Megophryidae, Anura). Zootaxa, 3147, 1–83.

Posada, D. & Crandall, K.A. (1998) Modeltest: testing the model of DNA substitution. Bioinformatics, 14 (9), 817–818.

Poyarkov, J.N.A., Orlov, N.L., Moiseeva, A.V., Pawangkhanant, P., Ruangsuwan, T., Vassilieva, A.B., Galoyan, E.A., Nguyen, T.T. & Gogoleva, S.S. (2015b) Sorting out moss frogs: mtDNA data on taxonomic diversity and phylogenetic relationships of the Indochinese species of the genus Theloderma (Anura, Rhacophoridae). Russian Journal of Herpetology, 22 (4), 241–280.

Poyarkov, J.N.A., Rowley, J.J., Gogoleva, S.I., Vassilieva, A.B., Galoyan, E.A. & Orlov, N.L. (2015a) A new species of Leptolalax (Anura: Megophryidae) from the western Langbian Plateau, southern Vietnam. Zootaxa, 3931 (2), 221–252.

Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19 (12), 1572–1574.

Rowley, J.J. & Cao, T.T. (2009) A new species of Leptolalax (Anura: Megophryidae) from central Vietnam. Zootaxa, 2198, 51–60.

Rowley, J.J., Dau, Q.V. & Nguyen, T.T. (2013) A new species of Leptolalax (Anura: Megophryidae) from the highest mountain in Indochina. Zootaxa, 3737 (4), 415–428.

Rowley, J.J., Hoang, H.D., Dau, V.Q., Le, D.T.T. & Cao, T.T. (2012) A new species of Leptolalax (Anura: Megophryidae) from central Vietnam. Zootaxa, 3321, 56–68.

Rowley, J.J., Hoang, H.D., Le, D.T.T., Dau, V.Q. & Cao, T.T. (2010c) A new species of Leptolalax (Anura: Megophryidae) from Vietnam and further information on Leptolalax tuberosus. Zootaxa, 2660, 33–45.

Rowley, J.J., Le, D.T.T., Tran, D.T.A. & Hoang, H.D. (2011) A new species of Leptolalax (Anura: Megophryidae) from southern Vietnam. Zootaxa, 2796, 15–28.

Rowley, J.J., Stuart, B.L., Neang, T. & Emmett, D.A. (2010a) A new species of Leptolalax (Anura: Megophryidae) from northeastern Cambodia. Zootaxa, 2567, 57–68.

Rowley, J.J., Stuart, B.L., Neang, T., Hoang, H.D., Dau, V.Q. & Nguyen, T.T. (2015b) A new species of Leptolalax (Anura: Megpphryidae) from Vietnam and Cambodia. Zootaxa, 4039 (3), 401–417.

Rowley, J.J., Stuart, B.L., Richards, S.J., Phimmachak, S. & Sivongxay, N. (2010b) A new species of Leptolalax (Anura: Megophryidae) from Laos. Zootaxa, 2681, 35–46.

Rowley, J.J., Tran, D.T.A., Frankham, G.J., Dekker, A.H., Le, D.T.T., Nguyen, T.Q., Dau, V.Q. & Hoang, H.D. (2015a) Undiagnosed cryptic diversity in small, microendemic frogs Leptolalax from the Central Highlands of Vietnam. PLoS ONE, 10 (5), e0128382.

Rowley, J.J., Tran, D.T.A., Le, D.T.T., Dau, V.Q., Peloso, P.L.V., Nguyen, T.Q., Hoang, H.D., Nguyen, T.T. & Ziegler, T. (2016) Five new, microendemic Asian Leaf-litter Frogs (Leptolalax) from the southern Annamite mountains, Vietnam. Zootaxa, 4085 (1), 063−102.

Rowley, J.J., Dau, V.Q., Hoang, H., Le, D.T., Cutajar, T.P. & Nguyen, T. (2017a) A new species of Leptolalax (Anura: Megophryidae) from northern Vietnam. Zootaxa, 4243 (3), 544–564.

Rowley, J.J., Dau, V.Q. & Cao, T.T. (2017b) A new species of Leptolalax (Anura: Megophryidae) from Vietnam. Zootaxa, 4273 (1), 061–079.

Stamatakis, A. (2014) RAxML Version 8: A tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics, 30 (9), 1312–3.

Taylor, E.H. (1962) The amphibian fauna of Thailand. The University of Kansas science bulletin, 43, 265−599.

Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. & Higgins, D.G. (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic acids research, 25 (24), 4876−4882.

Tran, V.T. (1995) Geological and minerals resources map of Vietnam: Huong Hoa-Hue-Da Nang part. In: Nguyen, T.V., Cat, N.H., Dang, V.B. & Do, H.N. (Eds.), Geological and minerals resources map of Vietnam on 1:200,000. Vietnam Derpartment of of Geology and Minerals, Hanoi, pp. 169.

Vences, M., Thomas, M., Bonett, R.M. & Vieites, D.R. (2005a) Deciphering amphibian diversity through DNA barcoding: chances and challenges. Philosophical Transactions of the Royal Society of London B: Biological Sciences, 360 (1462), 1859−1868.

Vences, M., Thomas, M., Van der Meijden, A., Chiari, Y. & Vieites, D.R. (2005b) Comparative performance of the 16S rRNA gene in DNA barcoding of amphibians. Frontiers in Zoology, 2 (1), 5.

Vieites, D.R., Wollenberg, K.C., Andreone, F., Köhler, J., Glaw, F. & Vences, M. (2009) Vast underestimation of Madagascar's biodiversity evidenced by an integrative amphibian inventory. Proceedings of the National Academy of Sciences, 106 (20), 8267−8272.

Walker, J.D., Geissman, J.W., Bowring, S. & Babcock, L. (2013) The Geological Society of America geologic time scale. Geological Society of America Bulletin, 125 (3–4), 259–272.



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