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Issue: Vol. 4365 No. 2: 18 Dec. 2017
Type: Article
Published: 2017-12-17
DOI: 10.11646/zootaxa.4365.2.1
Page range: 101–131
Abstract views: 127
PDF downloaded: 93

Leaf insects from Luzon, Philippines, with descriptions of four new species, the new genus Pseudomicrophyllium, and redescription of
Phyllium (Phyllium) geryon Gray, 1843, (Phasmida: Phylliidae)

Entomology Department, San Diego Natural History Museum, POB 121390, Balboa Park, San Diego, California, 92112-1390 USA Associate researcher with the Montreal Insectarium (Quebec-Canada)
Biology Department, City College of New York, City University of New York, 160 Convent Ave. New York, New York, United States. 10031
Biology Department, City College of New York, City University of New York, 160 Convent Ave. New York, New York, United States. 10031 Biology Ph.D. Program, Graduate Center, City University of New York, 365 Fifth Ave. New York, New York, 10016, United States Entomology Section, National Museum of the Philippines, Manila, 1000, Philippines
Phasmatodea DNA barcodes Philippines Luzon new species Phylliinae holotype Phyllium Mountain Province leaf insect

Abstract

Examination of unidentified Phylliidae specimens revealed a number of undescribed species from the island of Luzon, Philippines. Morphological and molecular study of specimens from the obscure phasmid genus Microphyllium Zompro, 2001, revealed a new species, which we describe as Microphyllium haskelli Cumming sp. nov.. It is here described and differentiated from the two other species in the genus, both currently only known from adults of a single sex. Pseudomicrophyllium Cumming gen. nov. is described as a new genus within Phylliidae with the type species Pseudomicrophyllium faulkneri Cumming gen. et sp. nov. as the sole known species in the genus. As is unfortunately often the case in the leaf-mimicking family Phylliidae, this new genus and species is only known from a single specimen. In addition to the new genus, two new Phyllium (Phyllium) species from the siccifolium species-group are named and described as Ph. (Ph.) antonkozlovi Cumming sp. nov. and Ph. (Ph.) bourquei Cumming & Le Tirant sp. nov.. In addition to the newly described species, Phyllium (Phyllium) geryon Gray, 1843 is redescribed from a nearly perfect specimen, completing some of the morphological knowledge gaps currently missing because of the severely damaged holotype specimen. A key to all known species of Phylliidae from Luzon is included. Holotype specimens for all four new species will be deposited in the National Museum of the Philippines type collection and paratype specimens will be deposited into the San Diego Natural History Museum collection or retained within the first author’s collection.

 

References

  1. Brock, P.D., Büscher, T. & Baker, E. (2017) Phasmida Species File Online. Version 5.0/5.0. Available from: http://Phasmida.SpeciesFile.org (accessed 20 September 2017)

    Collins, M. & Morris, M. (1985) Threatened Swallowtail Butterflies of the World, The IUCN Red Data Book. IUCN, Gland, 401 pp.

    Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature Methods, 9 (8), 772.

    https://doi.org/10.1038/nmeth.2109

    Edgar, R.C. (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research, 32 (5), 1792–1797.

    https://doi.org/10.1093/nar/gkh340

    Folmer, O., Black, M., Hoch, W., Lutz, R. & Vrijenhock, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3 (5), 294–299.

    Goloboff, P., Farris, J.S., Källersjö, M., Oxelmann, B., Ramirez, M. & Szumik, C. (2003) Improvements to resampling measures of group support. Cladistics, 17, S26–S34.

    https://doi.org/10.1111/j.1096-0031.2003.tb00376.x

    Goloboff, P.A., Farris, J.S. & Nixon, K.C. (2008) TNT, a free program for phylogenetic analysis. Cladistics, 24 (5), 774–786.

    https://doi.org/10.1111/j.1096-0031.2008.00217.x

    Gouy, M., Guindon, S. & Gascuel, O. (2010) SeaView version 4: a multiplatform graphical user interface for sequence alignment and phylogenetic tree building. Molecular Biology and Evolution, 27 (2), 221–224.

    https://doi.org/10.1093/molbev/msp259

    Gray, G.R. (1843) Description of several species of the Genus Phyllium. Zoologist, 1 (1), 117–123.

    Grösser, D. (2011) New Insights and Critical Remarks on certain species of Walking Leaves. ARTHROPODA Generalis, 3, 1–17.

    Haan, W. de (1842) Bijdragen tot de Kennis der Orthoptera. Verhandelingen over de natuurlijke Geschiedenis der Nederlandsche overzeesche Bezittingen. In: Temminck, C.J. (Ed.), Verhandelingen Zoologie, 2, pp. 95–138.

    Hennemann, F.H., Conle, O.V., Gottardo, M. & Bresseel, J. (2009) On certain species of the genus Phyllium Illiger, 1798, with proposals for an intra-generic systematization and the descriptions of five new species from the Philippines and Palawan (Phasmatodea: Phylliidae: Phylliinae: Phylliini). Zootaxa, 2322, 1–83.

    Hurst, G.D.D. & Jiggins, F.M. (2005) Problems with mitochondrial DNA as a marker in population, phylogeographic and phylogenetic studies: the effects of inherited symbionts. Proceedings of the Royal Society B: Biological Sciences, 272 (1572), 1525–1534.

    https://doi.org/10.1098/rspb.2005.3056

    Illiger, J.K.W. (1798) Verzeichnis der Käfer Preussens. Johann Jacob Gebauer, Halle, 510 pp.

    Kodandaramaiah, U., Simonsen, T.J., Bromilow, S., Wahlberg, N. & Sperling, F. (2013) Deceptive single-locus taxonomy and phylogeography: Wolbachia-associated divergence in mitochondrial DNA is not reflected in morphology and nuclear markers in a butterfly species. Ecology and Evolution, 3 (16), 5167–5176.

    https://doi.org/10.1002/ece3.886

    Kômoto, N., Yukuhiro, K., Ueda, K. & Tomita, S. (2011) Exploring the molecular phylogeny of phasmids with whole mitochondrial genome sequences. Molecular Phylogenetics and Evolution, 58 (1), 43–52.

    https://doi.org/10.1016/j.ympev.2010.10.013

    Linnæus, C. (1758) Systema naturae per regna tria naturae, secundum classes, ordines, genera, species, cum characteribus, differentiis, synonymis, locis. Editio decima, reformata. Laurentii Salvii, Stockholm, 824 pp.

    Lit, I. & Eusebio, O. (2014) A new species of leaf insect (Phasmatodea: Phylliidae) from Northern Luzon, Philippines. Arthropoda Generalis, 5, 1–11.

    Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. Gateway Computing Environments Workshop (GCE). California, La Jolla. Available from: http://www.phylo.org/sub_sections/portal/sc2010_paper.pdf (accessed 17 September 2017)

    Pérez-Ruiz, M., Martínez-Rodríguez, P., Herranz, J. & Bella, J.L. (2015) A survey of Wolbachia, Spiroplasma and other bacteria in parthenogenetic and non-parthenogenetic phasmid (Phasmatodea) species. European Journal of Entomology, 112 (3), 409–418.

    https://doi.org/10.14411/eje.2015.061

    Regier, J.C. & Shi, D. (2005) Increased yield of PCR product from degenerate primers with nondegenerate, nonhomologous 5’ tails. BioTechniques, 38 (1), 34–38.
    https://doi.org/10.2144/05381BM02

    Rehn, J.A.G. & Rehn, J.W.H. (1934[1933]) On certain species of the genus Phyllium (Orthoptera; Phasmidae). Proceedings of the Academy of Natural Sciences of Philadelphia, 85, 411–427.

    Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61 (3), 539–542.

    https://doi.org/10.1093/sysbio/sys029

    Rubinoff, D., Cameron, S. & Will, K. (2006) A genomic perspective on the shortcomings of mitochondrial DNA for “barcoding” identification. Journal of Heredity, 97 (6), 581–594.

    https://doi.org/10.1093/jhered/esl036

    Song, H., Buhay, J.E., Whiting, M.F. & Crandall, K.A. (2008) Many species in one: DNA barcoding overestimates the number of species when nuclear mitochondrial pseudogenes are coamplified. Proceedings of the National Academy of Sciences of the United States of America, 105 (36), 13486–13491.

    https://doi.org/10.1073/pnas.0803076105

    Swofford, D.L. (2002) PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods). Version 4. Sinauer Associates, Sunderland, Massachusetts. [software]

    Wahlberg, N. & Wheat, C. (2008) Genomic Outposts Serve the Phylogenomic Pioneers: Designing Novel Nuclear Markers for Genomic DNA Extractions of Lepidoptera. Systematic Biology, 57 (2), 231–242.

    https://doi.org/10.1080/10635150802033006

    Wedmann, S., Bradler, S. & Rust, J. (2007) The first fossil leaf insect: 47 million years of specialized cryptic morphology and behavior. The National Academy of Sciences of the USA, 104 (2), 565–569.

    https://doi.org/10.1073/pnas.0606937104

    Wells, J.D., Pape, T. & Sperling, F.A. (2001) DNA-based identification and molecular systematics of forensically important Sarcophagidae (Diptera). Journal of Forensic Science, 46 (5), 1098–1102.
    https://doi.org/10.1520/JFS15105J

    Westwood, J.O. (1859) Catalogue of Orthopterous insects in the collection of the British Museum. Part 1, Phasmidae. Order of the Trustees, British Museum, London, 195 pp.

    Zompro, O. (2001) Philippine phasmids from the collection of the Staatliches Museums fur Tierkunde, Dresden (Insecta: Phasmatodea). Reichenbachia, 34 (5), 49–56.