Abstract
Two new species of the Liolaemus donosobarrosi clade are described. Liolaemus tirantii sp. nov. and Liolaemus calliston sp. nov. differ from other members of their clade by a combination of coloration characters, morphometric and molecular traits. Liolaemus tirantii sp. nov. is known from three localities separated only a few kilometers from each other and Liolaemus calliston sp. nov. is known only from the type locality. Both species inhabit a region strongly impacted by oil and gas extraction but their conservation status is unknown.
References
Abdala, C.S. (2002) Nuevo Liolaemus (Iguania: Liolaemidae) perteneciente al grupo boulengeri de la provincia de Neuquén, Argentina. Cuadernos de Herpetología, 16, 3–13.
Abdala, C.S. (2007) Phylogeny of the boulengeri group (Iguania: Liolaemidae, Liolaemus) based on morphological and molecular characters. Zootaxa, 1538, 1–84.
Abdala, C.S. & Juarez, R. (2006) Geographic distribution notes: Liolaemus donosobarrosi. Herpetological Review, 37, 107.
Avila, L.J. (1996) Liolaemus donosobarrosi: Ampliación de su distribución geográfica y primera cita para la provincia de Neuquén. Cuadernos de Herpetología, 9, 109–110.
Avila, L.J., Morando, M. & Sites, J.W. Jr. (2006) Congeneric phylogeography: hypothesizing species limits and evolutionary processes in Patagonian lizards of the Liolaemus boulengeri group (Squamata: Liolaemini). Biological Journal of the Linnean Society, 89, 241–275.
https://doi.org/10.1111/j.1095-8312.2006.00666.x
Avila, L.J., Pérez, C.H.F., Medina, C.D., Sites, J.W. Jr. & Morando, M. (2012) A new species of lizard of the Liolaemus elongatus clade (Reptilia: Iguania: Liolaemini) from Curi Leuvu River Valley, northern Patagonia, Neuquén, Argentina. Zootaxa, 3325, 37–52.
Avila, L.J., Medina, C.D., Pérez, C.H.F., Sites, J.W.,Jr. & Morando, M. (2015) Molecular phylogenetic relationships of the Liolaemus elongatus clade (Iguania: Liolaemini) and a new species of lizard from an isolated volcanic peak in northern Patagonia. Zootaxa, 3947 (1), 67–84.
https://doi.org/10.11646/zootaxa.3947.1.4
Blum, R. (2012) Morphometric analyses and intraspecific comparison of two geographically separated populations of Liolaemus cuyanus (Iguania: Liolaemidae) from Northwestern Argentina. Unpublished Master Thesis Georg-August-Universität Göttingen. Germany, 101 pp.
Breitman, M.F., Avila, L.J., Sites, J.W. Jr. & Morando, M. (2011) Lizards from the end of the world: phylogenetic relationships of the Liolaemus lineomaculatus section (Squamata: Iguania: Liolaemini). Molecular Phylogenetics and Evolution, 59, 365–376.
https://doi.org/10.1016/j.ympev.2011.02.008
Breitman, M.F., Avila, L.J., Sites, J.W. Jr. & Morando, M. (2011) How lizards survived blizzards: phylogeography of the Liolaemus lineomaculatus group (Liolaemidae) reveals multiple breaks and refugia in southern Patagonia, and their concordance with other co-distributed taxa. Molecular Ecology, 21, 6068–6085.
https://doi.org/10.1111/mec.12075
Breitman, M.F., Morando, M. & Avila, L.J. (2013) Past and present taxonomy of the Liolaemus lineomaculatus section (Liolaemidae): is the morphological arrangement hypothesis valid? Zoological Journal of the Linnean Society, 168, 612–668.
https://doi.org/10.1111/zoj.12037
Cei, J.M. (1986) Reptiles del centro, centro-oeste y sur de la Argentina. Museo Regionale di Scienze Naturali di Torino Monografie, 4, 1–527.
Cei, J.M. (1993) Reptiles del noroeste, nordeste y este de la Argentina. Museo Regionale di Scienze Naturali di Torino, Monografie 14, 1–949.
Cei, J.M. (1974) Two new species of Ctenoblepharis (Reptilia, Iguanidae) from the arid environments of the Central Argentina (Mendoza Province). Journal of Herpetology, 8, 71–75.
https://doi.org/10.2307/1563078
Cei, J.M. (1979) A reassessment of the genus Ctenoblepharis (Reptilia, Sauria, Iguanidae) with the description of a new spubspecies of Liolaemus multimaculatus from Western Argentina. Journal of Herpetology, 13, 297–302.
https://doi.org/10.2307/1563323
Cei, J.M. & Roig, V. (1976) Fauna y ecosistemas del oeste árido argentino. I.-Reptiles de la provincia de Mendoza. Deserta, 4, 69–91.
Claude, J. (2008) Morphometrics with R. XVIII. Springer, New York, U.S.A. 316 pp.
Conover, W.J. (1999) Practical Nonparameteric Statistics. Third Edition, Wiley, U.S.A. 592 pp.
Crawley, M.J. (2007) The R Book. John Wiley & Sons Ltd, England.
http://dx.doi.org/10.1007/s00362-008-0118-3
Das, I. & Grismer, L.L. (2003) Two new species of Cnemaspis strauch, 1887 (Squamata: Gekkonidae) from the Seribuat archipelago, Pahang and Johor states, west Malaysia. Herpetologica, 59, 544–552.
https://doi.org/10.1655/02-22
De Queiroz, K. (1998) The general lineage concept of species, species criteria, and the process of speciation. In: Howard, D.J. & Berlocher, S.H. (Eds.), Endless Forms: Species and Speciation. Oxford University Press, Oxford, USA, pp. 57–75.
De Mendiburu, F. (2017) agricolae: statistical procedures for agricultural research. R package version 1.2-4. Available from: http://CRAN.R-project.org/package=agricolae (accessed 16 June 2017)
Drummond A.J. & Rambaut, A. (2007) BEAST: Bayesian evolutionary analysis by sampling trees. BMC Evolutionary Biology, 7, 214.
https://doi.org/10.1186/1471-2148-7-214
Etheridge, R.E. & Christie, M. (2003) Two new species of the lizard genus Liolaemus (Squamata: Liolaemidae) from northern Patagonia, with comments on Liolaemus rothi. Journal of Herpetology, 37, 325–341.
https://doi.org/10.1670/0022-1511(2003)037[0325:TNSOTL]2.0.CO;2
Fox, J. & Weisberg, S. (2011) An R Companion to Applied Regression. Second. Sage, Thousand Oaks CA. Available from: http://socserv.socsci.mcmaster.ca/jfox/Books/Companion (accessed 13 February 2017)
Frost, D.R. (1992) Phylogenetic analysis and taxonomy of the Tropidurus group of lizards (Iguania, Tropiduridae). American Museum Novitates, 3033, 1–68.
Gastwirth, J.L., Gel, Y.R., Hui, W.L.W., Lyubchich, V., Miao, W. & Noguchi, K. (2017) CRAN - Package lawstat. Available from: https://cran.r-project.org/web/packages/lawstat/index.html (accessed June 16 2017)
Guindon, S. & Gascuel, O. (2003) A simple, fast and accurate method to estimate large phylogenies by maximum-likelihood. Systematic Biology, 52, 696–704.
https://doi.org/10.1080/10635150390235520
Harlow, L. (2005) The essence of multivariate thinking: Basic themes and methods. Lawrence Erlbaum Associates, Mahwah, New Jersey.
http://dx.doi.org/10.1177/1094428107310086.Heath, L., Van der Walt, E., Varsani, A. & Martin D (2006) Recombination patterns in aphthoviruses mirror those found in other picornaviruses. Journal of Virology, 80, 11827–11832.
https://doi.org/10.1128/JVI.01100-06
Hothorn, T., Bretz, F., Westfall, P., Heiberger, R.M., Schuetzenmeister, A. & Scheibe, S. (2016) CRAN - Package multcomp. Available from: https://cran.r-project.org/web/packages/multcomp/index.html (accessed 16 June 2017)
Huelsenbeck, J.P. & Ronquist, F. (2001) MrBayes: Bayesian inference of phylogeny. Bioinformatics, 17, 754–755.
https://doi.org/10.1093/bioinformatics/17.8.754
Husson, F., Josse, J., Le, S. & Mazet, J. (2017) FactoMineR: Multivariate exploratory data analysis and data mining. R package version 1.36. Available from: https://CRAN.R-project.org/package=FactoMineR (accessed 4 June 2017)
Kassambara, A. & Mundt, F. (2017) factoextra: Extract and visualize the results of multivariate data analyses. R package version 1.0.4.999. Available from: http://www.sthda.com/english/rpkgs/factoextra (accessed 20 February 2017)
Kiviat, E. (2013) Risks to biodiversity from hydraulic fracturing for natural gas in the Marcellus and Utica shales. Annals of the New York Academy of Sciences, 1286, 1–14.
https://doi.org/10.1111/nyas.12146
Köhler, G. (2012) Color catalogue for field biologists. Herpeton Eds., Offenbach, Germany, 49 pp.
Lê, S., Josse, J. & Husson, F. (2008) FactoMineR: An R package for multivariate analysis. Journal of statistical software, 25, 1–18.
https://doi.org/10.18637/jss.v025.i01
Luo, R., Misra, M. & Himmelblau, D.M. (1999) Sensor fault detection via multiscale analysis and dynamic PCA. Industrial & Engineering Chemistry Research, 38, 1489–1495.
https://doi.org/10.1021/ie980557b
Martin, D. & Rybicki, E. (2000) RDP: detection of recombination amongst aligned sequences. Bioinformatics, 16, 562–563.
https://doi.org/10.1093/bioinformatics/16.6.562
Medina, C.D., Avila, L.J., Sites, J.W. Jr & Morando, M. (2017) Phylogeographic study of the Patagonian lizard complex Liolaemus elongatus (Iguania: Liolaemini) based on mitochondrial and nuclear evidence. Journal of Zoological Systematics and Evolutionary Research, 55, 238–249.
https://doi.org/10.1111/jzs.12163
Miller, J. & Haden, P. (2006) Statistical analysis with the General Linear Model. Creative Commons Attribution, USA. Available from: http://www.uv.es/~friasnav/librofactorial.pdf (accessed 29 April 2013)
Minoli, I., Morando, M. & Avila, L.J. (2014) Integrative taxonomy in the Liolaemus fitzingerii complex (Squamata: Liolaemini) based on morphological analyses and niche modeling. Zootaxa, 3856 (4), 501–528.
https://doi.org/10.11646/zootaxa.3856.4.3
Morando, M., Avila, L.J. & Sites, J.W. Jr. (2003) Sampling strategies for delimiting species: genes, individuals, and populations in the Liolaemus elongatus-kriegi complex (Squamata: Liolaemidae) in Andean-Patagonian South America. Systematic Biology, 52, 159–185.
https://doi.org/10.1080/10635150390192717
Olave, M., Martínez, L.E., Avila, L.J., Sites, J.W. Jr. & Morando, M. (2011) Evidence of hybridization in the Argentinean lizards Liolaemus gracilis and L. bibronii (Iguania: Liolaemini): an integrative approach based on genes and morphology. Molecular Phylogenetic and Evolution, 61, 381–391.
https://doi.org/10.1016/j.ympev.2011.07.006
Olave, M., Martínez, L.E., Avila, L.J., Sites, J.W. Jr. & Morando, M. (2014) Multilocus phylogeny of the widely distributed South American lizard clade Eulaemus (Liolaemini, Liolaemus). Zoologica Scripta, 43, 323–337.
https://doi.org/10.1111/zsc.12053
Olave M., Avila, L.J., Sites, J.W. Jr. & Morando, M. (2017) Hidden diversity within the lizard genus Liolaemus: genetic vs morphological divergence in the rothi complex (Squamata:Liolaeminae). Molecular Phylogenetics and Evolution, 107, 56–63.
https://doi.org/10.1016/j.ympev.2016.09.009
O’Rourke, N. & Hatcher, L. (2013) A step-by-step approach to using SAS for Factor Analysis and Structural Equation Modeling. Second Edition. SAS Institute, Cary, North Carolina, USA.
https://doi.org/10.2307/1270628
R Core Team (2017) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. Available from: http://www.R-project.org/ (accessed 20 March 2017)
Rambaut, A. & Drummond, A.J. (2009) Tracer v1.4, Available from http://beast.bio.ed.ac.uk/Tracer (Accessed 6 Dec. 2017)
Roig, A.F. (1998) La vegetación de la Patagonia. In: Correa, M.N. (Ed.), Flora Patagónica. INTA, Buenos Aires, Argentina. pp. 48–174.
Ronquist, F. & Huelsenbeck, J.P. (2003) Mr Bayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572–1574.
https://doi.org/10.1093/bioinformatics/btg180
Scrocchi, G.J., Abdala, C.S., Nori, J. & Zaher, H. (2010) Reptiles de la provincial de Rio Negro, Argentina. Fondo Editorial Rionegrino, Viedma. 252 pp.
Shepard, D.B., Irwin, K.J. & Burbrink, F.T. (2011) Morphological Differentiation in Ouachita Mountain Endemic Salamanders. Herpetologica, 67, 355–368.
https://doi.org/10.1655/HERPETOLOGICA-D-11-00023.1
Simmons, J.E. (2015) Herpetological collecting and collections managements. Herpetological Circular Society for the Study of Amphibians and Reptiles, 42, 191 pp.
Smith, H.M. (1946) Handbook of lizards. Comstock Publishing Company, Ithaca, New York, U.S.A, 557 pp.
Wickham, H. (2009) ggplot2: Elegant Graphics for Data Analysis. Springer-Verlag, New York. Available from: http://ggplot2.org (Accessed 6 Dec. 2017)
Yandell, B.S. (1997) Practical data analysis for designed experiments. Chapman & Hall.
https://doi.org/10.1007/978-1-4899-3035-4Zar, J.H. (2010) Biostatistical analysis. Prentice-Hall/Pearson, Upper Saddle River, N.J., 944 pp.