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Type: Article
Published: 2016-09-26
Page range: 401–450
Abstract views: 84
PDF downloaded: 2

Morphology and COI barcodes reveal four new species in the lycieus group of Calisto (Lepidoptera, Nymphalidae, Satyrinae)

Research Associate, National Museum of Natural History, Santo Domingo, Dominican Republic.: 7620 SW 142 Avenue, Miami, FL 33183.
Departamento de Colecciones Zoológicas y Sistemática, Instituto de Ecología y Sistemática, Carretera de Varona km 3. 5, Capdevila, Boyeros, CP 11900.La Habana, Cuba. Current address: Zoologische Staatssammlung Muenchen, Münchhausenstraße 21, 81247 München.
Research Associate. Florida State Collection of Arthropods, Division of Plant IndustryP.O. Box 147100/1911, SW 34 St., Gainesville, FL 32614-7100, U.S.A.
Lepidoptera Greater Antilles Hispaniola Jamaica COI ABGD barcoding Bayesian Inference Maximum Likelihood new species phylogeny monophyly diagnostic sites divergent morphology

Abstract

The predominantly Greater Antillean endemic genus Calisto Hübner, 1823 is highly diversified on several islands being more species rich on Hispaniola. We conducted expeditions during five years in the Dominican Republic resulting in new findings related with lyceius species group. Material belonging to this group was examined following the traditional morphological characters employed in genus taxonomy, and the COI barcode sequences obtained were analyzed through different approaches: Neighbor Joining clustering, ABGD, Maximum Likelihood (ML), and Bayesian Inference (BI). Analysis yielded 12 groups representing putative species: eight corresponding to previously named ones and four new species which are described in the present work: C. mariposa sp. nov., C. azua sp. nov., C. victori sp. nov., and C. samana sp. nov. The results also confirmed a single taxonomic entity within C. pulchella Lathy and the conspecific nature of C. franciscoi Gali and C. hendersoni. A dichotomic key for identification of species within the group is also given. Both phylogenetic reconstruction methods (ML and BI) employing molecular data achieved similar results with the relationships among the majority of taxa being supported by some ecological and morphological features. The exceptions were C. zangis Fabricius, C. raburni Gali, and C. pulchella, grouped together in a weakly supported clade. These species possess a highly differentiated adult and immature morphology which indicates an earlier divergence.

 

References

  1. Aliabadian, M., Nijman, V., Mahmoudi, A., Naderi, M., Vonk, R. & Vences, M. (2014) ExcaliBAR: a simple and fast software utility to calculate intra- and interspecific distances from DNA barcodes. Contributions to Zoology, 83 (1), 79–83.

    Bates, M. (1935) The satyrid genus Calisto. Occasional Papers Boston Society of Natural History, 8, 229–248.

    Benson, D.A., Clark, K., Karsch-Mizrachi, I., Lipman, D.J., Ostell, J. & Sayers, E.W. (2014) GenBank. Nucleic Acids Research, 42, 32–37.
    http://dx.doi.org/10.1093/nar/gkt1030

    Brower, A.V.Z. (2010) Alleviating the taxonomic impediment of DNA barcoding and setting a bad precedent: names for ten species of ‘Astraptes fulgerator’ (Lepidoptera: Hesperiidae: Eudaminae) with DNA–based diagnoses. Systematics and Biodiversity, 8 (4), 485–491.
    http://dx.doi.org/10.1080/14772000.2010.534512

    Brown, F.M. & Heineman, B. (1972) Jamaica and its butterflies. London, E. W. Classey Ltd, xv + 478 pp.

    Clench, H.K. (1943) Some new Calisto from Hispaniola and Cuba (Lepidoptera: Satyridae). Psyche, 50 (1/2), 23–29.
    http://dx.doi.org/10.1155/1943/45897

    Gali, F. (1985) Five new species of Calisto (Lepidoptera: Satyridae) from Hispaniola. Milwaukee Public Museum Contributions to Biology and Geology, 63, 1–16.

    Gonzalez, F.L., Schwartz, A. & Wetherbee, D.K. (1991) A new species of Calisto (Lepidoptera: Satyridae) of the lyceia complex on Hispaniola. Milwaukee Public Museum Contributions to Biology and Geology, 80, 1–8.

    Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98.

    Hamilton, C.A., Hendrixson, B.E., Brewer, M.S. & Bond, J.E. (2013) An evaluation of sampling effects on multiple DNA barcoding methods leads to an integrative approach for delimiting species: A case study of the North American tarantula genus Aphonopelma (Araneae, Mygalomorphae, Theraphosidae). Molecular Phylogenetics and Evolution, 71, 79–93.
    http://dx.doi.org/10.1016/j.ympev.2013.11.007

    Hebert, P.D.N., Cywinska, A., Ball, S.L. & deWaard, J.R. (2003) Biological identifications through DNA barcodes. Proceedings of the Royal Society of London. Series B, Biological Sciences, 270, 313–321.
    http://dx.doi.org/10.1098/rspb.2002.2218

    Hebert, P.D.N., Penton, E.H., Burns, J.M., Janzen, D.H. & Hallwachs, W. (2004) Ten Species in One: DNA Barcoding Reveals Cryptic Species in the Neotropical Skipper Butterfly Astraptes fulgerator. Proceedings of the National Academy of Sciences of the United States of America, 101 (41), 14812–14817.
    http://dx.doi.org/10.1073/pnas.0406166101

    Hedges, S. & Johnson, B.K. (1994) Calisto tasajera in the Hispaniolan Cordillera Central (Lepidoptera: Nymphalidae: Satyrinae). Tropical Lepidoptera, 5 (2), 93–94.

    Johnson, K. & Hedges, S.B. (1998) Three new species of Calisto from Southwestern Haiti (Lepidoptera: Nymphalidae: Satyrinae). Tropical Lepidoptera, 9 (2), 45–53.

    Johnson, K., Quinter, E.L. & Matusik, D. (1987) A new species of Calisto from Hispaniola with a review of the female genitalia of Hispaniolan congeners (Satyridae). Journal of Research on the Lepidoptera, 25 (2), 73–82.

    Kimura, M. (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16, 111–120.
    http://dx.doi.org/10.1007/BF01731581

    Lamas, G. (Ed.) (2004) Checklist: Part 4A. Hesperioidea-Papilionoidea. Atlas of Neotropical Lepidoptera. Association for Tropical Lepidoptera/Scientific Publishers, Gainesville, 439 pp.

    Lanfear, R., Calcott, B., Ho, S.Y.W. & Guindon, S. (2012) Partition Finder: Combined Selection of Partitioning Schemes and Substitution Models for Phylogenetic Analyses. Molecular Biology and Evolution, 29 (6), 1695–1701.
    http://dx.doi.org/10.1093/molbev/mss020

    Librado, P. & Rozas, J. (2009) DnaSP v5: A software for comprehensive analysis of DNA polymorphism data. Bioimformatics, 25, 1451–1452.
    http://dx.doi.org/10.1093/bioinformatics/btp187

    Matos-Maraví, P.F., Núñez, R., Peña, C., Miller, J.Y., Sourakov, A. & Wahlberg, N. (2014) Causes of endemic radiation in the Caribbean: evidence from the historical biogeography and diversification of the butterfly genus Calisto (Nymphalidae: Satyrinae: Satyrini). BMC Evolutionary Biology 14 (199), 1–18.
    http://dx.doi.org/10.1186/s12862-014-0199-7

    Michener, C.D. (1943) A review of the genus Calisto (Lepidoptera, Satyrinae). American Museum Novitates, 1236, 1–6.

    Munroe, E.G. (1951) The systematics of Calisto (Lepidoptera, Satyrinae), with remarks on the evolutionary and zoogeographic significance of the genus. Journal of the New York Entomological Society, 58 (4), 211–241.

    Núñez, R., Matos-Maraví, P.F. & Wahlberg, N. (2013) New Calisto species from Cuba, with insights on the relationships of Cuban and Bahamian taxa (Lepidoptera, Nymphalidae, Satyrinae). Zootaxa, 3669 (4), 503–521.
    http://dx.doi.org/10.11646/zootaxa.3669.4.5

    Núñez, R., Oliva, E., Matos-Maraví, P.F. & Wahlberg, N. (2012) Cuban Calisto (Lepidoptera, Nymphalidae, Satyrinae), a review based on morphological and DNA data. ZooKeys, 165, 57–105.
    http://dx.doi.org/10.3897/zookeys.165.2206

    Paz, A. & Crawford, A.J. (2012) Molecular–based rapid inventories of sympatric diversity: A comparison of DNA barcode clustering methods applied to geography–based vs clade–based sampling of amphibians. Journal of Biosciences, 37 (5), 887–896.
    http://dx.doi.org/10.1007/s12038-012-9255-x

    Puillandre, N., Lambert, A., Brouillet, S. & Achaz, G. (2011) ABGD, Automatic Barcode Gap Discovery for primary species delimitation. Molecular Ecology, 21 (8), 1864–1877.
    http://dx.doi.org/10.1111/j.1365-294X.2011.05239.x

    Puillandre, N., Modica, M.V., Zhang, Y., Sirovich, L., Boisselier, M.C., Cruaud, C., Holford, M. & Samadi, S. (2012) Large-scale species delimitation method for hyperdiverse groups. Molecular Ecology, 21 (11), 2671–2691.
    http://dx.doi.org/10.1111/j.1365-294X.2012.05559.x

    Pyrcz, T.W. (2010) Wybrane zagadnienia z taksonomii, zoogeografii i ewolucji faun gĂłrskich na przykĹ ‚adzie grupy modelowej motyli z plemienia Pronophilini (Nymphalidae). Wydawnictwo Mantis, 2010, 1–245.

    Rach, R., DeSalle, R., Sarkar, I.N., Schierwater, B. & Hadrys, H. (2008) Character–based DNA barcoding allows discrimination of genera, species and populations in Odonata. Proceedings of the Royal Society of London, Series B (Biological Sciences), 275, 237–247.
    http://dx.doi.org/10.1098/rspb.2007.1290

    Ratnasingham, S. & Hebert, P.D.N. (2013) A DNA–Based Registry for All Animal Species: The Barcode Index Number (BIN) System. PLoS ONE, 8 (8), 1–16.
    http://dx.doi.org/10.1371/journal.pone.0066213

    Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61 (3), 539–542.
    http://dx.doi.org/10.1093/sysbio/sys029

    Schwartz, A. (1989) The butterflies of Hispaniola. University of Florida Press, Gainesville, Florida, xiv + 580 pp.

    Smith, D.S., Miller, L.D. & McKenzie, F. (1991) The butterflies of Anegada, British Virgin Islands, with descriptions of a new Calisto (Satyridae) and a new Copaeodes (Hesperiidae) endemic to the island. Bulletin of the Allyn Museum, 133, 1–25.

    Smith, D.S., Miller, L.D. & Miller, J.Y. (1994) The Butterflies of the West Indies and South Florida. Oxford University Press, New York, 284 pp.

    Sourakov, A. (1996) Notes on the genus Calisto, with descriptions of the immature stages (part 1) (Lepidoptera: Nymphalidae: Satyrinae). Tropical Lepidoptera, 7 (1), 91–111.

    Sourakov, A. (1997) Morphology of immature stages in satyrinae (Lepidoptera: Nymphalidae) and its application to systematics. Ph.D. Dissertation. University of Florida, Gainesville, Florida, 135 pp.

    Sourakov, A. (2000) Notes on the genus Calisto, with descriptions of the immature stages (part 2) (Lepidoptera: Nymphalidae: Satyrinae). Tropical Lepidoptera, 10 (2), 73–79.

    Sourakov, A. (2007) Dominican Republic—notes on evolution of butterflies and of our knowledge about them. News of Lepidopterists’ Society, 49 (2), 46–55.

    Sourakov, A. & Zakharov, E.V. (2011) “Darwin’s butterflies”? DNA barcoding and the radiation of the endemic Caribbean butterfly genus Calisto (Lepidoptera, Nymphalidae, Satyrinae). Comparative Cytogenetics, 5 (3), 191–210.

    http://dx.doi.org/10.3897/CompCytogen.v5i3.1730

    Srivathsan, A. & Meier, R. (2011) On the inappropriate use of Kimura–2–parameter (K2P) divergences in the DNA–barcoding literature. Cladistics, 27, 1–5.

    Stamatakis, A., Hoover, P. & Rougemont, J. (2008) A rapid bootstrap algorithm for the RAxML web-servers. Systematic Biology, 75, 758–771.
    http://dx.doi.org/10.1080/10635150802429642

    Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. & Kumar, S. (2011) MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution, 28 (10), 2731–2739.
    http://dx.doi.org/10.1093/molbev/msr121

    Tavares, E.S. & Baker, A.J. (2008) Single mitochondrial gene barcodes reliably identify sister–species in diverse clades of birds. BMC Evolutionary Biology, 8, 81.
    http://dx.doi.org/10.1186/1471-2148-8-81

    The Canadian Centre for DNA Barcoding (CCDB) (2013) Available: from: http://www.ccdb.ca/resources.php (accessed 13 August 2016)

    Wisor, R.W. & Schwartz, A. (1985) Status of Calisto pulchella darlingtoni Clench (Lepidoptera: Satyridae). Florida Scientist, 48 (1), 7–13.