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Type: Articles
Published: 2011-05-19
Page range: 52–64
Abstract views: 38
PDF downloaded: 1

"Same-same, but different": an unusual new species of the Limnonectes kuhlii Complex from West Sumatra (Anura: Dicroglossidae)

University of Kansas Biodiversity Institute, 1345 Jayhawk Boulevard, Lawrence, Kansas 66045-7561, USA
University of Kansas Biodiversity Institute, 1345 Jayhawk Boulevard, Lawrence, Kansas 66045-7561, USA
University of Kansas Biodiversity Institute, 1345 Jayhawk Boulevard, Lawrence, Kansas 66045-7561, USA
University of Kansas Biodiversity Institute, 1345 Jayhawk Boulevard, Lawrence, Kansas 66045-7561, USA
Department of Biology, FMIPA Institut Teknologi Bandung, 10 Jalan Ganesa, Bandung 40132, Indonesia
Amphibia dicroglossine Limnonectes sisikdagu sp. nov. mitochondrial DNA morphology species complex

Abstract

A new species in the dicroglossine genus Limnonectes from West Sumatra, Indonesia, is described. Analysis of DNA sequence data from the mitochondrial 12S and 16S gene regions places the species within the Limnonectes kuhlii Complex and demonstrates it to be the sister taxon of Limnonectes kuhlii sensu stricto from Java. Both molecular and morphological data support the recognition of this lineage as a new species. Notably, the presence of a spinule-covered mental plate distinguishes Limnonectes sisikdagu sp. nov. from all other members of the L. kuhlii Complex. Additionally, pair-wise sequence divergence greater than 10% separate the new species from its sister taxon, L. kuhlii from Java.

References

  1. Alberch, P. & Gale, E. (1985) A Developmental Analysis of an Evolutionary Trend: Digital Reduction in Amphibians. Evolution, 39, 8–23.

    Bain, R. & Truong, N. (2004) Herpetofaunal diversity of Ha Giang Province in northeastern Vietnam, with descriptions of two new species. American Museum Novitates, 3453, 1–42.

    Berry, P.Y. (1975) The Amphibian Fauna of Peninsular Malaysia. Kuala Lumpur: Tropical Press, 130 pp.

    Boulenger, G.A. (1920) A monograph of the South Asian, Papuan, Melanesian and Australian frogs of the genus Rana. Records of the Indian Museum, 20, 1–223.

    Che, J., Pang, J., Zhao, H., Wu, G., Zhao, E. & Zhang, Y. (2007) Molecular phylogeny of the Chinese ranids inferred from nuclear and mitochondrial DNA sequences. Biochemical Systematics and Ecology, 35, 29–39.

    de Queiroz, K. (1998) The general lineage concept of species, species criteria, and the process of speciation: a conceptual unification and terminological recommendations. In: D. J. Howard & S. H. Berlocher (Eds), Endless forms: species and speciation. Oxford University Press, Oxford, pp. 57–75.

    de Queiroz, K. (1999) The General Lineage Concept of Species and the Defining Properties of the Species Category. In: R. A. Wilson (Ed.), Species: New Interdisciplinary Essays, MIT Press, Cambridge, Massachusetts, Pp. 4989.

    Doan, T. & Castoe, T. (2003) Using Morphological and Molecular Evidence to Infer Species Boundaries within Proctoporus bolivianus Werner (Squamata: Gymnopthalmidae). Herpetologica, 59, 432–449.

    Duméril, A.M.C. & Bibron, G. (1841) Erpetétology Général ou Histoire Naturelle complète des Reptiles. Paris: Libraire Encyclopédique de Roret, 792 pp.

    Emerson, S., Inger, R. & Iskandar, D. (2000) Molecular systematics and biogeography of the fanged frogs of Southeast Asia. Molecular Phylogenetics and Evolution, 16, 131–142.

    Evans, B.J., Brown, R.M., Mcguire, J.A., Supriatna, J., Andayani, N., Diesmos, A., Iskandar, D., Melnick, D. & Canatella, D. (2003) Phylogenetics of Fanged Frogs: Testing Biogeographical Hypotheses at the Interface of the Asian and Australian Faunal Zones. Systematic Biology, 52, 794–819.

    Fouquet, A., Vences, M., Salducci, M. & Meyer, A. (2007) Revealing cryptic diversity using molecular phylogenetics and phylogeography in frogs of the Scinax ruber and Rhinella margaritifera species groups. Molecular Phylogenetics and Evolution, 43, 567-–582.

    Frost, D.R., Grant, T., Faivovich, J., Bain, R.H., Haas, A., Haddad, C.F.B., De Sa, R.O., Channing, A., Wilkinson, M., Donnellan, S.C., Raxworthy, C.J., Campbell, J.A., Blotto, B.L., Moler, P., Drewes, R.C., Nussbaum, R.A., Lynch, J.D., Green, D., & Wheeler, W.C. (2006) The amphibian tree of life. Bulletin of the American Museum of Natural History, 297, 1–291.

    Hertwig, S., De Sa, R. & Haas, A. (2004) Phylogenetic signal and the utility of 12S and 16S mtDNA in frog phylogeny. Journal of Zoological Systematics and Evolutionary Research, 42, 2–18.

    Inger, R. (1966) The systematics and zoogeography of the amphibia of Borneo. Fieldiana: Zoology, 52, 1-–402.

    Inger, R, Stuart, B. & Iskandar, D.T. (2009) Systematics of a widespread Southeast Asian frog, Rana chalconota (Amphibia: Anura: Ranidae). Zoological Journal of the Linnean Society, 155, 123–147.

    Iskandar, D.T. (1998) The amphibians of Java and Bali. Research and Development Center for Biology–LIPI, 117 pp.

    Jiang, J. & Zhou, K. (2005) Phylogenetic relationships among Chinese ranids inferred from sequence data set of 12 S and 16 S rDNA. Herpetological Journal, 15, 1–8.

    Katoh, K., Misawa, K., Kuma, K. & Miyata, T. (2002) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Research, 30, 3059–3066.

    Malkmus, R., Manthey, U., Vogel, G., Hoffmann, P. & Kosuch, J. (2002) Amphibians and Reptiles of Mount Kinabalu (North Borneo). Ruggel: A.R.G. Ganter Verlag Kommanditgesellschaft, 424 pp.

    Matsui, M., Kuraishi, N., Jiang, J.-P., Ota, H., Hamidy, A., Orlov, N.L. & Nishikawa, K. (2010) Systematic reassessments of fanged frogs from China and adjacent regions (Anura: Dicroglossidae). Zootaxa, 2345, 33–42.

    Mayr, E. (1943) Criteria of subspecies, species and genera in Ornithology. Annals of the New York Academy of Sciences, 44, 133–139.

    McLeod, D.S. (2008) A new species of big-headed, fanged dicroglossine frog (Genus Limnonectes) from Thailand. Zootaxa, 1807, 26–46.

    McLeod, D.S. (2010) Of Least Concern? Systematics of a cryptic species complex: Limnonectes kuhlii (Amphibia; Anura: Dicroglossidae). Molecular Phylogenetics and Evolution, 56, 991–1000.

    Miller, M., Holder, M., Vos, R., Midford, P., Liebowitz, T., Chan, L., Hoover, P. & Warnow, T. (2009) The CIPRES Portals. CIPRES. Available from: http://www.phylo.org/sub_sections/portal (12 October 2010).

    Pope, C. (1931) Notes on amphibians from Fukien, Hainan, and other parts of China. Bulletin of the American Museum of Natural History, 61, 397–611.

    Posada, D. & Crandall, K.A. (1998) Modeltest: testing the model of DNA substitution. Bioinformatics, 14, 817–818.

    Rambaut, A. (2002) Se-Al v2.0a11. Available from http://tree.bio.ed.ac.uk/software/seal/.

    Simpson, G.G. (1961) Principles of animal taxonomy. New York: Columbia University Press.

    Stamatakis, A. (2006) RAxML-VI-HPC: Maximum Likelihood-based Phylogenetic Analyses with Thousands of Taxa and Mixed Models. Bioinformatics, 22, 2688–2690.

    Taylor, E.H. (1962) The Amphibian Fauna of Thailand. The University of Kansas Science Bulletin, 43, 1–599.

    Tschudi, J.J.V. (1838) Classification der Batrachier, mit Berücksichtigung der fossilen Thiere dieser Abtheilung der Reptilien. Mem. Soc. Sci. Nat. Neuchâtel, 2.

    Vences, M., Thomas, M., Bonett, R. & Vieites, D. (2005) Deciphering amphibian diversity through DNA barcoding: chances and challenges. Philosophical Transactions B, 360, 1859–1868.

    Vences, M., Thomas, M. & Van der Meijden, A. (2005) Comparative performance of the 16 S rRNA gene in DNA barcoding of amphibians. Frontiers in Zoology, 2, 5.

    Vieites, D.R., Wollenberg, K.C., Andreone, F., Köhler, J., Glaw, F. & Vences, M. (2009) Vast underestimation of Madagascar's biodiversity evidenced by an integrative amphibian inventory. Proceedings of the National Academy of Sciences, USA, 106, 8267–8272.

    Wiley, E.O. (1978) The Evolutionary Species Concept Reconsidered. Systematic Zoology, 27, 17–26.

    Zhang, J., Nie, L., Peng, Q., Ge, Y., Wang, Y., Xu, J. & Tang, X. (2005) Relationships among the Chinese group of Limnonectes based on mitochondrial 12S and 16S rRNA sequences. Acta Zoologica Sinica, 51, 3–54.

    Zhang, J., Nie, L., Wang, Y. & Hu, L. (2009) The complete mitochondrial genome of the large-headed frog, Limnonectes bannaensis (Amphibia: Anura), and a novel gene organization in the vertebrate mtDNA. Gene, 442, 119–127.