Abstract
A new species of crocidurine shrew, Crocidura hikmiya, is described from the Sinharaja World Heritage Site, Sri Lanka. The species is diagnosed on the basis of both morphology and mitochondrial DNA sequence data. Morphologically C. hikmiya is distinguished from C. miya, among other characters, by having a shorter tail, condyles protruding beyond the margin of the braincase, a posterior edge of maxillary bone rounded (dorsal view), an occipital bone triangularly shaped with an obtuse angle (dorsal view) and slightly flattened on the back; a foramen magnum less deep (ventral view); a dorsal posterior brain case not smooth; and an angular process of dentary short and stout. Phylogenetic analysis suggests that C. hikmiya is the sister-species of C. miya. The uncorrected genetic distance between the two species for the mitochondrial cytochrome-b gene fragment is 9.7–10.1%, suggesting species-level divergence. Crocidura hikmiya is confined to the mid-montane forests and lowland rainforests in the southwestern Sri Lanka, while C. miya is confined to montane forests of the central hills.References
Asher, R.J. (1999) A morphological basis for assessing phylogeny of the “Tenrecoidea” (Mammalia, Lipotyphla). Cladistics, 15, 231–251.
Bahir, M.M. & Surasinghe, T. (2005) A conservation assessment of the Sri Lankan Agamidae (Reptilia: Sauria). In: Yeo, C.J., Ng, P.K.L. & Pethiyagoda, R. (Ed.), Contributions to biodiversity exploration and research in Sri Lanka. The Raffles Bulletin of Zoology, Supplement 12, 407–412.
Bahir, M.M., Ng, P.K.L., Crandall, K. & Pethiyagoda, R. (2005) A conservation assessment of the freshwater crabs of Sri Lanka. In: Yeo, C. J., Ng, P. K. L. & Pethiyagoda, R. (Ed.), Contributions to biodiversity exploration and research in Sri Lanka. The Raffles Bulletin of Zoology, Supplement 12, 121–126.
Bahir, M.M. & Maduwage, K.P. (2005) Calotes desilvai, a new species of agamid lizard from Morningside Forest, Sri Lanka, In: Yeo, C. J., Ng, P. K. L. & Pethiyagoda, R. (Ed.), Contributions to biodiversity exploration and research in Sri Lanka. The Raffles Bulletin of Zoology, Supplement 12, 381–392.
Bahir, M.M. & Ng, P.K.L. (2005) Descriptions of ten new species of freshwater crabs (Crustacea: Brachyura: Parathelphusidae: Ceylonthelphusa, Mahatha, Perbrinckia) from Sri Lanka. In: Yeo, C. J., Ng, P. K. L. & Pethiyagoda, R. (Ed.), Contributions to biodiversity exploration and research in Sri Lanka. The Raffles Bulletin of Zoology, Supplement 12, 47–76.
Batuwita, S. & Bahir, M.M. (2005) Description of five new species of Cyrtodactylus (Reptilia: Gekkonidae) from Sri Lanka. In: Yeo, C. J., Ng, P. K. L. & Pethiyagoda, R. (Ed.), Contributions to biodiversity exploration and research in Sri Lanka. The Raffles Bulletin of Zoology, Supplement 12, 351–380.
Bossuyt F., Meegaskumbura, M., Beenaerts, N., Gower, D. J., Pethiyagoda, R., Roelants, K., Mannaert, A., Wilkinson, M., Bahir, M. M., Manamendra-Arachchi, K., Ng, P.K.L., Schneider, C.J., Oommen, O.V. & Milinkovitch, M.C. (2004) Local endemism within the western Ghats-Sri Lanka Biodiversity hotspot. Science, 306, 479–481.
Bradley, R.D. & Baker, R.J. (2001) A test of the genetic species concept: cytochrome b sequences and mammals. Journal of Mammalogy, 82, 960–973.
Corbet, G.B. & Hill, J.E. (1992) The mammals of the Indomalayan Region: a systematic review. Oxford University Press, Oxford. viii+488 pp.
Fernando, P., Dayawansa, N. & Siriwardena, M. (1994) Microhyla karunaratnei (Anura: Microhylidae), a new species of frog endemic to Sri Lanka. Journal of South Asian Natural History, 2, 135–142.
Huelsenbeck, J.P. & Ronquist, F. (2001) MRBAYES: Bayesian inference of phylogeny. Bioinformatics, 17, 754–755.
Hutterer, R. (2005) Order Soricomorpha. In: Wilson D. E. & Reeder, D. M. (Ed.), Mammal species of the world: a taxonomic and geographic reference. 3 ed. The Johns Hopkins University Press, Baltimore, pp. 220–311.
Jeanmougin F., Thompson, J.D., Gouy, M., Higgins, D.G. & Gibson, T.J. (1998) Multiple sequence alignment with Clustal X. Trends in Biochemical Sciences, 23, 403–405
Johns, G.C. & Avise, J.C. (1998) A comparative summary of genetic distances in the vertebrates from the mitochondrial cytochrome b gene. Molecular Biology and Evolution, 15, 1481–1490.
Manamendra-Arachchi, K. & Pethiyagoda, R. (2001) Polypedates fastigo, a new tree frog (Ranidae: Rhacophorinae) from Sri Lanka. Journal of South Asian Natural History, 5, 191–199.
Manamendra-Arachchi, K. & Pethiyagoda, R. (2005) The Sri Lankan shrub frogs of the genus Philautus Gistel, 1848 (Ranidae: Rhacophorinae), with description of 27 new species. In: Yeo, C. J., Ng, P. K. L. & Pethiyagoda, R. (Ed.), Contributions to biodiversity exploration and research in Sri Lanka. The Raffles Bulletin of Zoology, Supplement 12, 163–303.
Meegaskumbura, M. & Manamendra-Arachchi, K. (2005) Description of eight new species of shrub-frogs (Ranidae: Rhacophorinae: Philautus) from Sri Lanka. In: Yeo, C. J., Ng, P. K. L. & Pethiyagoda, R. (Ed.), Contributions to biodiversity exploration and research in Sri Lanka. The Raffles Bulletin of Zoology, Supplement 12, 305–338.
Meegaskumbura, M., Bossuyt, F., Pethiyagoda, R., Manamendra-Arachchi, K., Bahir, M., Milinkovitch, M.C. & Schneider, C.J. (2002) Sri Lanka: an amphibian hotspot. Science, 298, 379.
Pethiyagoda, R. & Manamendra-Arachchi, K. (1998) A revision of the endemic Sri Lankan Agamid Lizard genus Ceratophora Gray, 1835, with description of two new species. Journal of South Asian Natural History, 3, 1–50.
Phillips, W.W.A. (1929) A new and rare Ceylon shrew. Spolia Zelanica, 15, 113.
Phillips, W.W.A. (1980) A manual of the mammals of Sri Lanka. Wildlife and Nature Protection Society of Sri Lanka, Colombo. 389+xxxv pp.
Posada, D. & Crandall, K.A. (1998) Modeltest: testing the model of DNA substitution. Bioinformatics, 14, 817–818.
Queiroz, K. de (1998) The general lineage concept of species. In: Howard, D. J. & Berlocher, S. H. (Ed.), Endless forms: species and speciation. Oxford University Press, Oxford. Pp. 57–75.
Rambaut, A. (1996) Se-Al: Sequence Alignment Editor. Available from http://evolve.zoo.ox.ac.uk/ (accessed 31 January 2007).
Sites, J.W. Jr. & Crandall, K.A. (1997) Testing species boundaries in biodiversity studies. Conservation Biology. 11, 1289–1297.
Stanhope, M.J., Waddell, V.G., Madsen, O., de Jong, W., Blair Hedges, S., Cleven, G.C., Kao, D. & Springer, M. (1998) Molecular evidence for multiple origins of Insectivora and for a new order of endemic African insectivore mammals. Proceedings of the National Academy of Sciences, U.S.A., 95, 9967–9972.
Swofford, D.L. (2000) PAUP* 4.0 Phylogenetic analysis using parsimony (and other methods). Sinauer Associates, Sunderland. CD-ROM.
Wijesinghe, M.R. & Brooke, M.deL. (2005) Impact of habitat disturbance on the distribution of endemic species of small mammals and birds in a tropical rain forest in Sri Lanka. Journal of Tropical Ecology, 21, 661–668.
Whitemore, T.C. (1984) Tropical rain forests of the Far East. Clarendon Press, Oxford. 352 pp.